Table S3 Pathology Notes Describing Histological Assessment of Ciz1-/- Mice from Supplemental
CIZ1 at the inactive X
Table S3 Pathology notes describing histological assessment of Ciz1-/- mice from Supplemental Table 2.
Animal / Genotype / Organ / Pathology1 / -/- / Lungs / Prominent infiltrate of small to medium sized lymphocytes around bronchi, bronchioles and vessels, extending from the hilum to the periphery of the lung. The lung parenchyma, bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains two large, nodular aggregates of small to medium sized lymphocytes adjacent to a large hepatic vein. Occasional, small parenchymal foci of lymphocytes and neutrophils are present. The background liver architecture, hepatocytes and bile ducts appear normal.
Spleen / The spleen shows expansion of the white pulp by small to medium sized lymphocytes.
Lymph nodes / Very large lymph nodes with effaced architecture and a nodular infiltrate of small to medium sized lymphocytes with extranodal extension.
Thymus / The thymus contains a nodular infiltrate of small to medium sized lymphocytes.
Reproductive tract / Normal uterus, ovary and oviduct.
Kidney / Aggregate of small to medium sized lymphocytes with scanty plasma cells in renal pelvis, focal lymphoid aggregates in parenchyma. Glomeruli, tubules, and vessels normal.
Pancreas / The pancreas contains a small lymphoid aggregate by a vessel and islet but is otherwise normal.
Salivary gland / Not identified
Bladder / Not identified
2 / -/- / Lungs / The lungs contain a very prominent infiltrate of small to medium sized lymphocytes with larger blast-like cells in some areas. The infiltrate is present around bronchi, bronchioles and vessels, from hilum to lung periphery. Occasional granulomas are likely to be reactive. The lung parenchyma, bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains numerous nodular infiltrates of small to medium sized lymphocytes with occasional larger blast-like cells. The infiltrates are mostly in periportal locations. There are also many foci of granulomatous inflammation at the periphery of the nodules and within the liver parenchyma which may be reactive. The background liver architecture, hepatocytes and bile ducts appear normal.
Spleen / The spleen shows expansion of the white pulp by small to medium sized lymphocytes.
Lymph nodes / One of the lymph nodes shows effaced architecture with an infiltrate of small to medium sized lymphocytes. Occasional larger lymphocytes. Two other nodes show a nodular infiltrate including small to medium sized lymphoid cells with larger blast-like cells. Occasional granulomas are present.
Thymus / Not included
Reproductive tract / Normal uterus
Kidney / Hilar and cortical aggregates of medium sized lymphocytes, most adjacent to vessels. Glomeruli, tubules, and vessels normal.
Pancreas / The pancreas contains a small lymphoid aggregate by a vessel but is otherwise normal.
Salivary gland / Not identified
Bladder / Not identified
3 / -/- / Lungs / The lungs contain a moderate infiltrate of mostly small lymphocytes with occasional plasma cells around bronchi, bronchioles, vessels. The lung parenchyma, bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains occasional focal aggregates of lymphocytes with pigmented macrophages and neutrophils in periportal, parenchymal and perivenular areas. Some of these foci are granulomatous. No large nodular infiltrates are present. A single tiny focus of extramedullary haematopoiesis is present. The background liver architecture, hepatocytes and bile ducts appear normal.
Spleen / The white pulp appears normal but the red pulp is expanded by extensive extramedullary haematopoiesis.
Lymph nodes / Normal
Thymus / Normal
Reproductive tract / Not identified
Kidney / The kidney contains a single tiny aggregate of lymphocytes and plasma cells by vessels. Glomeruli, tubules, and vessels normal.
Pancreas / The pancreas contains a periductal infiltrate of lymphocytes and plasma cells with a small focus of destruction of periductal acini, although the majority of the gland is normal.
Salivary gland / Normal parotid, submandibular and sublingual glands.
Bladder / The bladder contains a moderate number of mucosal and serosal aggregates of lymphocytes and plasma cells. The wall and epithelium are normal.
4 / -/- / Lungs / The lungs contain an increased amount of lymphoid tissue around vessels and airways, consisting of small and medium sized lymphocytes with plasma cells. The lung parenchyma, bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains large and sometimes conflent nodular infiltrates of small to medium sized, and occasionally large lymphoid cells with scanty plasma cells. Multiple small foci of neutrophils and eosinophils are present within the parenchyma. The background liver architecture, hepatocytes and bile ducts appear normal.
Spleen / The spleen contains a nodular infiltrate of small to medium sized lymphoid cells with some larger lymphoid cells.
Lymph nodes / An enlarged parotid lymph node shows effaced architecture and is infiltrated by small lymphoid cells.
Thymus / Normal
Reproductive tract / Normal uterus
Kidney / Normal
Pancreas / Normal
Salivary gland / Sublingual and parotid glands normal. Submandibular gland not present.
Bladder / There are 2 aggregates of lymphocytes and plasma cells in the mucosa. The wall and epithelium are normal.
5 / -/- / Lungs / The lungs contain a slightly increased amount of lymphoid tissue around vessels and airways, consisting of small and medium sized lymphocytes with plasma cells. The lung parenchyma, bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains a single nodular infiltrate of small lymphoid cells. Occasional small foci of inflammatory cells are present within the parenchyma. The background liver architecture, hepatocytes and bile ducts appear normal.
Spleen / Normal
Lymph nodes / Not identified
Thymus / Normal
Reproductive tract / Normal uterus. One ovary contains a multiloculated cyst lined by serous type epithelium.
Kidney / Normal renal parenchyma with dilated renal pelvis, possibly hydronephrosis.
Pancreas / Normal
Salivary gland / Normal submandibular gland. Sublingual and parotid not identified.
Bladder / There are 3 aggregates of lymphocytes and plasma cells in the mucosa. The wall and epithelium are normal.
8 / -/- / Lungs / Prominent infiltrate of mostly small lymphocytes around vessels, less prominent around airways. In places medium sized and larger lymphoid cells, as seen in spleen, are also present. In some areas there are foamy macrophages in alveoli and an infiltrate of small to medium-sized lymphoid cells in the pleura. The bronchi and vessels are normal and there is no dysplasia or malignancy of the respiratory epithelium.
Liver / The liver contains small to large, nodular and confluent infiltrates of medium to large lymphoid cells in perivenular, periportal, and subcapsular areas with cells also filling sinusoids.
Spleen / The splenic architecture is effaced by an infiltrate of large lymphoid cells with frequent mitoses.
Lymph nodes / There are two or more lymph nodes with an infiltrate of mixed small to medium sized lymphoid cells. Two parotid nodes with a similar infiltrate also including plasma cells are present. There are several very large nodes containing an infiltrate of small to medium sized lymphoid cells with extranodal extension.
Thymus / Not identified
Reproductive tract / Not identified
Kidney / Normal glomeruli and tubules. There is a patchy interstitial infiltrate of medium to large lymphoid cells in the cortex and around vessels. The perinephric fat contains an infiltrate of small to medium sized lymphoid cells.
Pancreas / Normal
Salivary gland / There are occasional small lymphoid aggregates in the submandibular gland. Normal parotid and sublingual glands.
Bladder / Not identified