Supplementary Data

Table S1: Accesion numbers and primer sequences of studied genes.

Table S2: Correlation matrix for antioxidant enzyme transcripts in leaves. Spearman coefficient, statistically significant differences are indicated in bold (p < 0.001).

ERD1 / cAPX / sAPX / tAPX / CAT1 / CAT2 / CAT3 / MnSOD / FeSOD / CuZnSOD / variable
-0.27 / 0.05 / 0.25 / 0.09 / 0.48 / 0.09 / -0.06 / 0.52 / 0.28 / 0.02 / AtCKX1
0.03 / -0.35 / -0.35 / -0.57 / -0.03 / 0.1 / -0.48 / -0.52 / -0.16 / ERD1
-0.05 / -0.3 / 0.22 / -0.54 / 0.31 / -0.1 / -0.01 / 0.62 / cAPX
0.87 / 0.41 / 0.64 / 0.66 / 0.25 / 0.57 / 0.17 / sAPX
0.29 / 0.69 / 0.47 / 0.13 / 0.56 / -0.05 / tAPX
-0.09 / 0.1 / 0.38 / 0.57 / 0.16 / CAT1
0.33 / 0.18 / 0.14 / -0.34 / CAT2
-0.28 / 0.14 / 0.38 / CAT3
0.43 / 0.07 / MnSOD
0.29 / FeSOD

Table S3: Correlation matrix for antioxidant enzyme transcripts in roots. Spearman coefficient, statistically significant differences are indicated in bold (p < 0.001).

ERD1 / cAPX / sAPX / tAPX / CAT1 / CAT2 / CAT3 / MnSOD / FeSOD / CuZnSOD / variable
-0.35 / -0.09 / -0.32 / -0.16 / 0.09 / -0.14 / 0.05 / 0.05 / -0.21 / 0.22 / AtCKX1
0.05 / -0.3 / -0.05 / 0.14 / 0.01 / -0.27 / 0.16 / -0.24 / -0.23 / ERD1
0.19 / -0.1 / -0.25 / -0.26 / 0.08 / 0.14 / 0.58 / 0.64 / cAPX
0.75 / 0 / 0.34 / 0.65 / -0.17 / 0.61 / 0.13 / sAPX
0.24 / 0.42 / 0.39 / 0.09 / 0.22 / -0.13 / tAPX
-0.18 / 0.02 / 0.06 / -0.2 / -0.2 / CAT1
0.37 / 0.12 / 0.1 / -0.17 / CAT2
-0.04 / 0.35 / 0.31 / CAT3
-0.03 / -0.19 / MnSOD
0.28 / FeSOD

Supplementary figure legend

Figure S1. Transcript levels of AtCKX1 in leaves and roots of CKX1 transgenic tobacco plants. C - control (hydrated) conditions; D - drought stress (8-d dehydration); H - heat (40 °C for 2 h); D + H – combined stress (8-d dehydration + 40 °C for 2 h). Data represent mean values + SD from three biological repetitions. Symbol * indicates significant difference (p ≤ 0.05) between particular stress treatment and control conditions in the corresponding part and genotype.

Figure S2. The activity of antioxidant enzymes in upper, middle and lower leaves and roots of WT, W6:CKX1 and 35S:CKX1. (A) Ascorbate peroxidase (APX) was detected spectrophotometrically, (B) catalase (CAT) was measured polarographically, (C) superoxide dismutase (SOD) was determined spectrophotometrically. Other designation as described in Fig. S1.

Figure S3: Grouping of functionally specialized enzymes by Principal component analysis.