Supplemental Table 1 Heterozygous Deletion Strains with Secondary Mutations

Supplemental Table 1 Heterozygous deletion strains with secondary mutations

ORF / GENE / CRITERIA
YAL005C / ssa1 / mates as a
YBL021C / HAP3 / mates as an alpha
YBL011W / SCT1 / no sporulation
YBL003C / HTA2 / meiotic inviability
YBL022C / PIM1 / no sporulation
YBL005W / pdr3 / no sporulation
YBL002W / HTB2 / no sporulation
YBL091C / MAP2 / mates as an a
YBL069W / AST1 / no sporulation
YBL001C / ECM15 / no sporulation
YJL166W / QCR8 / mates as an a
YDR077W / SED1 / mates as an a
YDR516C / YDR516C / meiotic inviability
YBR129C / OPY1 / meiotic inviability
YDL144C / YDL144C / meiotic inviability
YER162C / rad4 / meiotic inviability
YGL006W / PMC1 / no sporulation
YDR033W / MRH1 / no sporulation
YGL232W / YGL232W / no sporulation
YER186C / YER186C / no sporulation
YOR202W / his3 / no sporulation
YNL202W / SPS19 / meiotic inviability

Supplemental Table 2 Yeast genes haploinsufficient in YPD

ORF / GENE / HET_AV
YKL054C / VID31 / 0.975
YDR394W / RPT3 / 0.972
YFR004W / RPN11 / 0.971
YGR255C / COQ6 / 0.960
YEL034W / HYP2 / 0.975
YDR429C / TIF35 / 0.944
YPL237W / SUI3 / 0.942
YJR007W / sui2 / 0.910
YMR309C / nip1 / 0.866
YLR249W / yef3 / 0.977
YJL102W / MEF2 / 0.938
YPL202C / YPL202C / 0.972
YDR145W / TAF61 / 0.971
YBR198C / TAF90 / 0.964
YBR154C / rpb5 / 0.917
YPR187W / RPO26 / 0.893
YOR224C / rpb8 / 0.877
YHR143W-A / RPC10 / 0.844
YDR308C / SRB7 / 0.969
YJL140W / RPB4 / 0.950
YDR404C / rpb7 / 0.940
YIL021W / RPB3 / 0.917
YIR005W / IST3 / 0.964
YDL043C / prp11 / 0.952
YBR265W / TSC10 / 0.946
YHR062C / RPP1 / 0.977
YKL009W / MRT4 / 0.958
YNL061W / NOP2 / 0.933
YNL075W / IMP4 / 0.966
YLR197W / SIK1 / 0.961
YHR148W / IMP3 / 0.960
YPR137W / RRP9 / 0.943
YBR279W / PAF1 / 0.965
YPR133C / YPR133C / 0.952
YOR206W / YOR206W / 0.960
YPL093W / NOG1 / 0.953
YNR053C / YNR053C / 0.975
YPR016C / TIF6 / 0.945
YFR001W / LOC1 / 0.962
YNL110C / YNL110C / 0.944
YLR009W / YLR009W / 0.942
YHR170W / NMD3 / 0.938
YDR184C / ATC1 / 0.976
YER165W / PAB1 / 0.964
YPL235W / RVB2 / 0.926
YLR403W / SFP1 / 0.965
YNR016C / ACC1 / 0.978
YML031W / ndc1 / 0.947
YMR091C / NPL6 / 0.939
YDR212W / tcp1 / 0.964
YJR064W / CCT5 / 0.957
YDR188W / CCT6 / 0.951
YDL143W / CCT4 / 0.942
YJL014W / CCT3 / 0.930
YIL142W / CCT2 / 0.887
YJL111W / CCT7 / 0.881
YBR173C / UMP1 / 0.958
YBR048W / RPS11B / 0.973
YLR167W / RPS31 / 0.973
YGR214W / RPS0A / 0.969
YML024W / RPS17A / 0.962
YGR118W / RPS23A / 0.959
YBR189W / RPS9B / 0.959
YJR145C / RPS4A / 0.958
YEL054C / RPL12A / 0.950
YLR448W / RPL6B / 0.950
YNL178W / RPS3 / 0.950
YKR094C / RPL40B / 0.943
YGL123W / RPS2 / 0.942
YDL130W / RPP1B / 0.941
YDR025W / RPS11A / 0.939
YDR382W / RPP2B / 0.936
YML073C / RPL6A / 0.921
YPR132W / RPS23B / 0.920
YGL030W / RPL30 / 0.907
YIL148W / RPL40A / 0.904
YPL131W / RPL5 / 0.892
YDL081C / RPP1A / 0.892
YDR418W / RPL12B / 0.848
YOL127W / RPL25 / 0.818
YGR034W / RPL26B / 0.977
YLR441C / RPS1A / 0.976
YDR405W / MRP20 / 0.975
YGR148C / RPL24B / 0.971
YNL096C / RPS7B / 0.970
YGL031C / RPL24A / 0.969
YPR043W / RPL43A / 0.967
YDL136W / RPL35B / 0.966
YLR388W / RPS29A / 0.965
YLR185W / RPL37A / 0.965
YMR230W / RPS10B / 0.960
YLR287C-A / RPS30A / 0.959
YBR084C-A / RPL19A / 0.958
YDL082W / RPL13A / 0.957
YDR471W / RPL27B / 0.955
YPL090C / RPS6A / 0.952
YOL121C / RPS19A / 0.951
YHR203C / RPS4B / 0.950
YER056C-A / RPL34A / 0.948
YBL027W / RPL19B / 0.947
YDR450W / RPS18A / 0.944
YIL018W / RPL2B / 0.943
YML063W / RPS1B / 0.937
YJL136C / RPS21B / 0.934
YDL061C / RPS29B / 0.926
YMR143W / RPS16A / 0.925
YER117W / RPL23B / 0.923
YOR312C / RPL20B / 0.923
YOR293W / RPS10A / 0.917
YMR242C / RPL20A / 0.912
YBR191W / RPL21A / 0.909
YHL015W / RPS20 / 0.907
YHR021C / RPS27B / 0.905
YER074W / RPS24A / 0.904
YLR061W / RPL22A / 0.897
YCL037C / SRO9 / 0.893
YDR064W / RPS13 / 0.893
YNL069C / RPL16B / 0.887
YDL191W / RPL35A / 0.886
YKL006W / RPL14A / 0.883
YOR096W / RPS7A / 0.872
YKL180W / RPL17A / 0.866
YIL052C / RPL34B / 0.865
YOL120C / RPL18A / 0.865
YDL075W / RPL31A / 0.861
YHR010W / RPL27A / 0.850
YMR142C / RPL13B / 0.847
YJR123W / RPS5 / 0.846
YOL040C / RPS15 / 0.843
YJL189W / RPL39 / 0.840
YPL143W / RPL33A / 0.812
YBR156C / SLI15 / 0.959
YGR274C / TAF145 / 0.943
YBR247C / ENP1 / 0.965
YML093W / YML093W / 0.970
YMR093W / YMR093W / 0.961
YLR222C / YLR222C / 0.958
YDR324C / YDR324C / 0.955
YDR449C / YDR449C / 0.944
YHR196W / YHR196W / 0.940
YAR002W / NUP60 / 0.896
YIR009W / MSL1 / 0.969
YLR275W / SMD2 / 0.954
YPR131C / NAT3 / 0.932
YGL092W / NUP145 / 0.976
YFR002W / NIC96 / 0.959
YKL057C / NUP120 / 0.862
YPL169C / MEX67 / 0.955
YLR115W / CFT2 / 0.962
YMR061W / RNA14 / 0.935
YNL118C / DCP2 / 0.973
YBR236C / ABD1 / 0.978
YML085C / TUB1 / 0.921
YFL008W / smc1 / 0.917
YNL126W / SPC98 / 0.961
YHR172W / SPC97 / 0.941
YER133W / GLC7 / 0.977
YIL126W / STH1 / 0.960
YBR133C / HSL7 / 0.925
YCR057C / PWP2 / 0.972
YER157W / SEC34 / 0.928
YPR181C / SEC23 / 0.864
YIR006C / PAN1 / 0.957
YHR081W / YHR081W / 0.977
YBL023C / mcm2 / 0.975
YBR202W / CDC47 / 0.955
YNL216W / rap1 / 0.977
YCR052W / RSC6 / 0.975
YPR034W / ARP7 / 0.964
YFL039C / act1 / 0.866
YBR078W / ECM33 / 0.949
YDR359C / VID21 / 0.974
YEL035C / UTR5 / 0.971
YPL146C / YPL146C / 0.971
YDR367W / YDR367W / 0.963
YBR267W / YBR267W / 0.960
YHR168W / YHR168W / 0.928
YDL193W / YDL193W / 0.898
YHR011W / DIA4 / 0.969
YGR095C / RRP46 / 0.967
YGR158C / MTR3 / 0.960
YCR035C / RRP43 / 0.957
YOL021C / DIS3 / 0.950
YHR069C / RRP4 / 0.902

Supplemental Table 3 Yeast genes with reduced fitness as homozygous deletions in YPD

Orf / gene / HOM_AV
YLR069C / MEF1 / 0.679
YDR347W / mrp1 / 0.699
YNL081C / YNL081C / 0.723
YHR168W / YHR168W / 0.723
YER050C / RSM18 / 0.727
YDR194C / MSS116 / 0.730
YLR061W / RPL22A / 0.732
YLR260W / LCB5 / 0.735
YMR188C / YMR188C / 0.735
YLR091W / YLR091W / 0.736
YMR193W / MRPL24 / 0.744
YIR026C / yvh1 / 0.746
YMR228W / mtf1 / 0.750
YGL246C / RAI1 / 0.752
YHR011W / DIA4 / 0.753
YKL170W / MRPL38 / 0.754
YMR293C / YMR293C / 0.755
YHL011C / PRS3 / 0.757
YPL040C / ISM1 / 0.757
YDR507C / GIN4 / 0.759
YMR282C / AEP2 / 0.759
YLL033W / YLL033W / 0.760
YDR529C / QCR7 / 0.761
YBR251W / MRPS5 / 0.762
YHR147C / MRPL6 / 0.764
YMR287C / MSU1 / 0.765
YDR065W / YDR065W / 0.766
YLR068W / YLR068W / 0.766
YML061C / pif1 / 0.768
YPR100W / YPR100W / 0.768
YGL076C / RPL7A / 0.769
YDR175C / RSM24 / 0.771
YMR267W / ppa2 / 0.774
YER056C-A / RPL34A / 0.775
YGL129C / RSM23 / 0.776
YHR021C / RPS27B / 0.777
YGR215W / RSM27 / 0.778
YLR322W / YLR322W / 0.779
YPL005W / YPL005W / 0.779
YOR211C / mgm1 / 0.781
YDR140W / YDR140W / 0.783
YDR405W / MRP20 / 0.783
YMR269W / YMR269W / 0.784
YER061C / CEM1 / 0.785
YOL023W / IFM1 / 0.785
YLR370C / ARC18 / 0.787
YER017C / AFG3 / 0.787
YLR067C / PET309 / 0.787
YMR097C / YMR097C / 0.788
YLR149C / YLR149C / 0.789
YOR295W / YOR295W / 0.789
YMR060C / TOM37 / 0.790
YLR439W / MRPL4 / 0.791
YOR035C / SHE4 / 0.791
YNL059C / ARP5 / 0.792
YBR267W / YBR267W / 0.792
YCR046C / IMG1 / 0.792
YHR120W / msh1 / 0.793
YMR125W / sto1 / 0.793
YGL218W / YGL218W / 0.793
YCL058C / YCL058C / 0.793
YHR010W / RPL27A / 0.794
YGR101W / YGR101W / 0.794
YMR116C / ASC1 / 0.794
YPL118W / MRP51 / 0.795
YKL006W / RPL14A / 0.796
YBR268W / MRPL37 / 0.796
YPL271W / ATP15 / 0.796
YGR162W / TIF4631 / 0.796
YDR268W / MSW1 / 0.796
YER077C / YER077C / 0.798
YDR241W / BUD26 / 0.798
YPL097W / MSY1 / 0.798
YCR071C / IMG2 / 0.798
YKL134C / 37165 / 0.799
YNL227C / YNL227C / 0.799
YER122C / GLO3 / 0.800
YLR270W / YLR270W / 0.802
YLR185W / RPL37A / 0.802
YPR132W / RPS23B / 0.802
YNL284C / MRPL10 / 0.803
YKL003C / mrp17 / 0.803
YHR041C / srb2 / 0.803
YMR142C / RPL13B / 0.804
YER028C / YER028C / 0.804
YJL188C / BUD19 / 0.805
YPL173W / MRPL40 / 0.805
YNL246W / YNL246W / 0.806
YLR139C / SLS1 / 0.806
YEL029C / BUD16 / 0.807
YDR332W / YDR332W / 0.807
YNL248C / RPA49 / 0.808
YEL050C / RML2 / 0.808
YJL102W / MEF2 / 0.808
YCR063W / YCR063W / 0.809
YDL061C / RPS29B / 0.809
YPL193W / RSA1 / 0.810
YHR081W / YHR081W / 0.810
YER014W / HEM14 / 0.810
YNL177C / YNL177C / 0.811
YMR089C / YTA12 / 0.811
YOL051W / gal11 / 0.811
YJL189W / RPL39 / 0.812
YPR101W / SNT309 / 0.812
YBR181C / RPS6B / 0.813
YLR403W / SFP1 / 0.814
YLR295C / ATP14 / 0.814
YLR435W / YLR435W / 0.815
YHR038W / FIL1 / 0.815
YNL073W / MSK1 / 0.815
YCR009C / RVS161 / 0.815
YDL160C / DHH1 / 0.815
YOL033W / MSE1 / 0.816
YLR192C / HCR1 / 0.816
YLR382C / nam2 / 0.816
YPL239W / YAR1 / 0.816
YDL081C / RPP1A / 0.817
YPR116W / YPR116W / 0.817
YNR036C / YNR036C / 0.818
YLL006W / MMM1 / 0.818
YEL044W / YEL044W / 0.819
YNL250W / rad50 / 0.819
YGL223C / YGL223C / 0.819
YNR052C / POP2 / 0.819
YGR104C / SRB5 / 0.819
YAR002W / NUP60 / 0.819
YKL057C / NUP120 / 0.819
YLR312W-A / MRPL15 / 0.820
YDR161W / TCI1 / 0.820
YPL125W / KAP120 / 0.820
YDR298C / ATP5 / 0.820
YOR205C / YOR205C / 0.821
YGL240W / DOC1 / 0.821
YPL106C / SSE1 / 0.821
YBL007C / SLA1 / 0.822
YDL191W / RPL35A / 0.822
YKL009W / MRT4 / 0.823
YER044C / BUD18 / 0.825
YKL138C / MRPL31 / 0.825
YOL009C / MDM12 / 0.826
YOR001W / RRP6 / 0.827
YDR115W / YDR115W / 0.827
YOR182C / RPS30B / 0.827
YML063W / RPS1B / 0.827
YPR131C / NAT3 / 0.828
YBR106W / PHO88 / 0.828
YGR285C / ZUO1 / 0.829
YBR191W / RPL21A / 0.829
YNL280C / ERG24 / 0.829
YKR085C / MRPL20 / 0.829
YBR200W / BEM1 / 0.830
YIR005W / IST3 / 0.831
YOR183W / YOR183W / 0.831
YDL044C / mtf2 / 0.831
YBR168W / YBR168W / 0.831
YIL093C / RSM25 / 0.832
YDR083W / YDR083W / 0.832
YDR126W / YDR126W / 0.832
YPL029W / suv3 / 0.832
YLR052W / YLR052W / 0.832
YDR388W / rvs167 / 0.833
YDR320C / SWA2 / 0.833
YGR171C / MSM1 / 0.833
YPR133W-A / TOM5 / 0.834
YFR001W / LOC1 / 0.834
YJR145C / RPS4A / 0.834
YPL101W / YPL101W / 0.835
YCR020W-B / HTL1 / 0.835
YOL095C / HMI1 / 0.836
YLR448W / RPL6B / 0.836
YHR034C / YHR034C / 0.836
YNL005C / MRP7 / 0.836
YMR219W / ESC1 / 0.837
YKL055C / OAR1 / 0.837
YDR519W / fkb2 / 0.838
YGL219C / YGL219C / 0.838
YDL056W / MBP1 / 0.838
YOR201C / pet56 / 0.839
YHR026W / ppa1 / 0.839
YJL136C / RPS21B / 0.839
YPL148C / PPT2 / 0.840
YNL069C / RPL16B / 0.840
YDR378C / LSM6 / 0.840
YKR020W / YKR020W / 0.840
YBL038W / MRPL16 / 0.840
YGR085C / RPL11B / 0.840
YHR059W / YHR059W / 0.841
YBL093C / ROX3 / 0.841
YJR055W / hit1 / 0.841
YHL031C / GOS1 / 0.841
YKL143W / LTV1 / 0.841
YPL183W-A / YPL183W-A / 0.842
YCR028C / FEN2 / 0.842
YML073C / RPL6A / 0.842
YOR008C / SLG1 / 0.842
YLR371W / ROM2 / 0.842
YER116C / YER116C / 0.842
YBR026C / MRF1' / 0.842
YHR194W / YHR194W / 0.843
YPL161C / BEM4 / 0.843
YHR067W / YHR067W / 0.844
YPR047W / MSF1 / 0.844
YJR063W / RPA12 / 0.844
YEL046C / GLY1 / 0.844
YLR423C / APG17 / 0.844
YMR224C / MRE11 / 0.844
YFL036W / RPO41 / 0.845
YLR193C / YLR193C / 0.845
YPL078C / ATP4 / 0.845
YJL096W / MRPL49 / 0.845
YPL188W / POS5 / 0.846
YJL046W / YJL046W / 0.846
YMR064W / AEP1 / 0.846
YDR174W / HMO1 / 0.846
YDL202W / MRPL11 / 0.846
YGR165W / YGR165W / 0.846
YEL054C / RPL12A / 0.846
YNL297C / MON2 / 0.847
YKR057W / RPS21A / 0.847
YER154W / oxa1 / 0.847
YJR118C / ILM1 / 0.847
YNL054W / VAC7 / 0.847
YFL001W / deg1 / 0.848
YGR167W / CLC1 / 0.848
YDR463W / STP1 / 0.848
YDL185W / TFP1 / 0.848
YMR091C / NPL6 / 0.848
YEL036C / anp1 / 0.849
YOR078W / BUD21 / 0.849
YLR240W / vps34 / 0.850
YJR032W / CPR7 / 0.851
YPL234C / TFP3 / 0.851
YNL079C / TPM1 / 0.851
YER014C-A / BUD25 / 0.851
YNL225C / CNM67 / 0.851
YOR141C / ARP8 / 0.852
YKR099W / bas1 / 0.852
YLR384C / IKI3 / 0.852
YDL082W / RPL13A / 0.852
YLL027W / ISA1 / 0.852
YPL178W / CBC2 / 0.852
YMR143W / RPS16A / 0.853
YOR096W / RPS7A / 0.853
YMR158W / YMR158W / 0.853
YBR163W / DEM1 / 0.854
YLR261C / YLR261C / 0.854
YDL106C / pho2 / 0.854
YKL114C / APN1 / 0.854
YDR418W / RPL12B / 0.854
YBR194W / YBR194W / 0.854
YKL016C / ATP7 / 0.855
YDR450W / RPS18A / 0.855
YJR104C / sod1 / 0.855
YGR150C / YGR150C / 0.856
YPL254W / HFI1 / 0.856
YKL054C / VID31 / 0.856
YMR072W / ABF2 / 0.857
YER095W / rad51 / 0.857
YHR064C / PDR13 / 0.857
YPL086C / ELP3 / 0.858
YML094W / GIM5 / 0.858
YLR287C-A / RPS30A / 0.858
YBL090W / MRP21 / 0.858
YPR043W / RPL43A / 0.858
YJR113C / RSM7 / 0.858
YLR200W / YKE2 / 0.859
YLR373C / VID22 / 0.859
YFL016C / MDJ1 / 0.859
YDR448W / ADA2 / 0.859
YDR377W / ATP17 / 0.859
YPR036W / vma13 / 0.859
YNL055C / POR1 / 0.860
YDR137W / rgp1 / 0.860
YOL076W / MDM20 / 0.860
YDR322W / MRPL35 / 0.860
YGL064C / YGL064C / 0.860
YFL018C / LPD1 / 0.860
YMR257C / pet111 / 0.860
YDR382W / RPP2B / 0.861
YML026C / rps18B / 0.861
YAR015W / ade1 / 0.861
YER110C / KAP123 / 0.861
YPR057W / BRR1 / 0.861
YNL229C / ure2 / 0.862
YMR179W / spt21 / 0.862
YML036W / YML036W / 0.862
YCR028C-A / rim1 / 0.862
YIL070C / MAM33 / 0.862
YMR312W / YMR312W / 0.862
YCL007C / CWH36 / 0.862
YBR048W / RPS11B / 0.863
YGR252W / GCN5 / 0.863
YDR237W / MRPL7 / 0.863
YHR100C / YHR100C / 0.863