Doug Hardesty
Earlham College
Drawer 936, 801 National Road W., Richmond, IN, 47374
Phone: (765) 620-9070
· EDUCATION
2003-present: Earlham College, Richmond, IN.
Bachelor of Arts with College Honors (expected 5/2007). Major: Chemistry; Minor: Biology.
Major GPA: 4.0; Cumulative GPA: 3.96.
2002-2003: Austin Community College, Austin, TX.
“Early College Start” program (dual-enrolled at college and high school).
Cumulative GPA: 4.0.
1999-2003: Austin Academy of the Arts (a secular homeschool), Austin, TX.
Largely self-designed, self-paced high school education.
G.P.A.: 4.14, Units: 36.5. SAT I: 1400 (750 Verbal, 650 Math, May 2002).
· RESEARCH EXPERIENCE
Correlations between feline follicular development and post-gonadectomy growth rates.
Anatomy/Physiology: Endocrinology and Metabolism independent project. Laboratory of Dr. David Matlack, Earlham College, Richmond, IN. Fall 2005.
Attack and counter-attack between viruses and the innate immune response: characterization of
the potential therapeutic target E3L. Laboratory of Dr. David Levy, New York University School of Medicine, New York, NY. June-August 2005.
Synthesis of phytosiderophore Avenic acid. Laboratory of Dr. Mark Stocksdale, Earlham College,
Richmond, IN. Fall 2004 and Spring 2005.
Quantification of trace elements in coffee beans. Equilibrium and Analysis independent project.
Laboratory of Dr. Paul Ogren, Earlham College, Richmond, IN. Spring 2005.
Organic synthesis of caffeine. Organic Chemistry II independent project. Laboratory of Dr. Mark
Stocksdale, Earlham College, Richmond, IN. Fall 2004.
K-ras modulates the Neurofibromatosis Type 1 phenotype in Mast Cells. Laboratory of Dr. D. Wade
Clapp, Indiana University School of Medicine, Indianapolis, IN. June-August 2004.
· WORK EXPERIENCE
Webmaster. Earlham College Chemistry Department website (http://www.earlham.edu/~chem).
Independently administer and update entire website. Spring 2005-present.
Teaching Assistant. Organic II Laboratory. Earlham College Chemistry Department. Prepared lab
equipment and materials; provided in-lab supervision and guidance to 14 students. Fall 2005.
Research Associate. Laboratory of Dr. David Levy. New York University School of Medicine. This work
was supported by the NYU Summer Undergraduate Research Program. June-August 2005.
Teaching Assistant. Organic I Laboratory. Earlham College Chemistry Department. Prepared lab
equipment and materials; graded quizzes; provided in-lab supervision and guidance to 16 students. Spring 2005.
Research Associate. Laboratory of Dr. D. Wade Clapp. Indiana University School of Medicine. This
work was supported by the Earlham College Howard Hughes Medical Institute grant. June-August 2004.
· PUBLISHED ABSTRACTS
Stocksdale, M.G.; Lindsay, A.M.; Hardesty, D.A.; Silver, M.K. (2005). Synthetic efforts toward novel phytosiderophore conjugates. Abstract # CHED-549. 229th National Meeting, American Chemical Society.
Khalaf, W.F.; Hardesty, D.A.; Chen, S.; Yang, F.C.; White, H.A.; Wenning, M.J.; Ingram, D.A.; Clapp
D.W. (2004). The Role of Neurofibromin as a GTPase Activating Protein for K-ras in Mast Cells. Abstract # 2560. 46th Annual Meeting, American Society of Hematology.
· PRESENTATIONS
Hardesty, D.A.; Marie, I.J.; Levy, D.E. Attack and counter-attack between viruses and the innate immune response: characterization of the potential therapeutic target E3L. Earlham College Biology Colloquium, Richmond, IN, March 2006.
Peoples, M. A.; Kinne, R. J.; Hardesty, D. A.; Stocksdale, M. G. Progress toward labeled phytosiderophore analogs (Poster). 6th Annual Indiana Local Section of the American Chemical Society Poster Session, October 2005, Indianapolis, IN; 3rd Annual Earlham Undergraduate Research Conference, Richmond, IN, November 2005.
Hardesty, D.A.; Marie, I.J.; Levy, D.E. Attack and counter-attack between viruses and the innate immune response: characterization of the potential therapeutic target E3L (Poster). NYU Undergraduate Research Presentation Conference, August 2005, New York, NY; 3rd Annual Earlham Undergraduate Research Conference, Richmond, IN October 2005.
Hardesty, D.A.; Marie, I.J.; Levy, D.E. Attack and counter-attack between viruses and the innate immune response: characterization of a potential therapeutic target. National Leadership Alliance Meeting, Danvers, MA, July 2005.
Stocksdale, M. G.; Lindsay, A. M.; Hardesty, D. A.; Silver, M. K. Synthetic efforts toward novel phytosiderophore conjugates (Poster). 229th National Meeting of the American Chemical Society, San Diego, CA, March 2005.
Hardesty, D.A.; Khalaf, W.F.; Clapp, D.W. K-ras Heterozygosity Modulates the Neurofibromatosis Type 1 Phenotype in Mast Cells (Poster). 2nd Annual Earlham College Undergraduate Research Conference, Richmond, IN, November 2004.
Hardesty, D.A.; Khalaf, W.F.; Clapp, D.W. K-ras Heterozygosity Modulates the Neurofibromatosis Type 1 Phenotype in Mast Cells. IU Summer Biomedical Research Program Meeting, August 2004, Indianapolis, IN; Earlham College Biology Colloquium, Richmond, IN, November 2004.
· SCIENCE COURSES
Chemistry:Principles of Chemistry (Fall 03, Lecture/Lab, A+)
Organic Chemistry I (Spring 04, Lecture/Lab, A+)
Organic Chemistry II (Fall 04, Lecture/Lab, A+)
Student Research (Fall 04, Lab, A)
Student Research (Spring 05, Lab, A)
Equilibrium / Analysis (Spring 05, Lecture/Lab, A)
Inorganic Chemistry (Fall 05, Lecture/Lab, A)
Advanced Organic Chemistry (Spring 06, Lecture/Lab, A) / Biology:
Ecological Biology (Fall 03, Lecture/Lab, A)
Humanity in Medicine (Fall 03, Seminar, A-)
Cells, Genes, and Inheritance (Spring 04, Lecture/Lab, A)
Cell Physiology (Fall 04, Lecture/Lab, A)
Anatomy and Physiology: Excitable Cells and Systems (Spring 05, Lecture/Lab, A+)
Anatomy and Physiology: Endocrinology and Metabolism (Fall 05, Lecture/Lab, A+)
Immunology (Fall 05, Lecture, A)
Parasitology (Spring 06, Lecture/Lab, A)
Mathematics:
Calculus A (Fall 04, A)
Calculus B (Spring 05, A) / Physics:
General Physics I (Fall 05, Lecture/Lab, A+)
General Physics II (Spring 06, Lecture/Lab, A+)
· VOLUNTEER SERVICE AND MEDICAL EXPERIENCES
Student Representative. Earlham College Curricular Policy Committee (CPC), Richmond, IN. The
academic faculty and student representatives of CPC oversee all general education requirements, changes to majors or minors, new majors or minors, and new course proposals. CPC is composed of the Associate Academic Dean, the Registrar, two faculty representatives from each division of the college, and three students. Spring 2005-present.
Veterinary Tech Volunteer. HELP Animal Shelter, Richmond, IN. Assisted in spaying and neutering
cats as part of an independent research project. Assisted in preparation, surgery, and post-operative care. Fall 2005.
Student Member. Earlham College Chemistry Department Faculty Search Committee, Richmond, IN.
Reviewed applications, attended candidate lectures, interviewed candidates, and provided recommendations for hiring. Fall 2005.
NSO Staff Volunteer. Earlham College New Student Orientation, Richmond, IN. Organized activities and
registration materials, greeted new students, and assisted students in moving into dorms. Also served as academic advisor aide to Lori Watson of the Chemistry Department; primary responsibility as advisor aide was to help first-year students register for classes. August 2005.
Student Observer. Office of the Chief Medical Examiner, New York, NY. Attended and assisted with
autopsies for a day. Moved/positioned cadavers for photographs, massed organs and recorded
weights, examined x-rays. June 2005.
Student Member. Earlham College Biology Department Faculty Search Committee, Richmond, IN.
Attended candidate lectures, interviewed candidates, and provided recommendations for hiring. Spring 2005.
Volunteer Science Fair Judge. Westview Elementary School, Richmond, IN. Judged 4th through 6th grade science projects on skill in applying the scientific method and lucidity of presentation and presentation materials. Spring 2004.
· AWARDS AND HONORS
Phi Beta Kappa Honor Society. Earlham College, 2006.
Russell M. Lawall Award in Biomedical Research. Earlham College, 2006.
Gertrude Pardieck Hubbard Distinguished Student in Chemistry Award. Earlham College, 2005.
Presidential Honor Roll. Austin Community College, 2002, 2003.
· ORGANIZATIONS
American Chemical Society
Earlham College Student Government
· RESEARCH SKILLS
Chemistry:Flash Chromatography
High Pressure Liquid Chromatography (HPLC)
Atomic Absorption/Graphite Furnace
Gas Chromatography/Mass Spectrometry/Electron Capture (GC/MS/EC)
Inductively Coupled Plasma Spectroscopy (ICP)
Infrared Spectroscopy
Nuclear Magnetic Resonance Spectroscopy (1H, 13C, DEPT, COSY, HETCOR, HMBC)
High-Pressure Synthesis
Inert-atmosphere Glovebox Synthesis
Analytical Titration
Biology:
Mammalian Tissue Culture
Cell Line Culture
Bacterial/Yeast Culture
Tissue Sectioning, Fixing, Staining
Gel Electrophoresis
Western Blotting
Green Fluorescent Protein Microscopy / Light Microscopy
Polymerase Chain Reaction (PCR)
Reverse Transcriptase PCR (RT-PCR)
DNA/RNA extraction
Gene Cloning / Vector Creation
Cell Line Transfection
RNAi Gene Silencing / Yeast and Bacterial Transformation
Yeast Two-Hybrid Assays
HIS3 Reporter Assays
B-Gal Reporter Assays
Flow Cytometry (FACS)
Reverse Transcriptase Activity Assay
Biochemical Activation Assays (Ras, JNK, ERK1/2)
Murine Surgery (microosmotic pump insertion)
Murine Fetal Liver Isolation
Murine Bone Marrow Extraction
Hemocytometry
3H-labeled Proliferation Assays
Histamine Degranulation Assays
Cell Migration Assays
Cadaver Dissection
· COMPUTER SKILLS
ChemDraw Ultra, Delta, SciFinder Scholar, Adobe Photoshop, Microsoft Excel/Word/PowerPoint, Macromedia Dreamweaver, SPSS, Maple 9.
· PERSONAL REFERENCES
Dr. Mark Stocksdale, Ph.D.
Associate Professor of Chemistry
Chemistry Department
Earlham College
801 National Rd. W., Richmond, IN, 47374.
Dr. Lori Watson, Ph.D.
Assistant Professor of Chemistry and Academic Advisor
Chemistry Department
Earlham College
801 National Rd. W., Richmond, IN, 47374.
Dr. Peter Blair, Ph.D.
Assistant Professor of Biology
Biology Department
Earlham College
801 National Rd. W., Richmond, IN, 47374.