Supplementary material - Results of the NCA computed on Calobiusquadricollis (a) and C. urbanelliae (b, c, d)
-step: 3-1 23-1 23-5 22-1 4-5 4-1 4-3 7-1 7-2 7-6 20-2 20-1 22-3 22-5 26-5 5-3 5-4 5-2 26-4 5-5 5-1 26-1 26-3 1-3 1-5 1-2 1-1

Dc: 331 0 0 0 0 248 0 249 298 0

Dn: 372 477 463 431 293 317 0 214 214 299

(I-T)c: 289 236

(I-T)n: - 108S - 75

1-step: 1.1 1.2 1.3 1.5 1.6 1.7 1.8 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20

Dc: 386 248 92 0 0 269 0 0 0

Dn: 374 301 89 100 84 267 309 265 265

(I-T)c: 138 92 269

(I-T)n: 73 0 - 12

2-step: 2.1 2.2 2.3 2.6 2.7 2.8

Dc: 374L 0S 0 0

Dn: 349L 233S 454 2 49

(I-T)c: 374L 0

(I-T)n: 115L 204

3-step: 3.1 3.2 3.3 3.4

Dc: 328 94S 290S 321

Dn: 359L 319 309 330L

(I-T)c: -233S - 31

(I-T)n: - 39S - 21S

4-step: 4.1 4.2

Dc: 346S 320S

Dn: 537S 750L

(I-T)c:

(I-T)n:

a – Results of the NCA computed on Calobiusquadricollis. Boxes reflect the nesting structure. Dc and Dn are respectively clade and nested clade distances, (Templeton et al, 1995). Interior vs tip contrasts for Dc and Dn are indicated with ‘I–T’ in the corresponding clade. Superscript S and superscript L indicate that distance measures are significantly smaller and larger, respectively, than expected under random distribution of haplotypes.

0-step: 14-3 14-5 16-1 14-4 16-4

Dc: 0.0 22.7

Dn: 25.5 23.5

(I-T)c: 22.7

(I-T)n: -2

1-step: 1.1 1.2 1.3 1.4

Dc: 25.5 0.0 0.0 0.0

Dn: 22.7 13.6 34.0 34.0

(I-T)c: -25.5 0.0

(I-T)n: -9.0 0.0

2-step: 2.1 2.2

Dc: 23.6 34.0

Dn: 21.4 19.4

(I-T)c: _

(I-T)n: _

b – Results of the NCA computed on Calobiusurbanelliae-Adriatic.Boxes reflect the nesting structure. Dc and Dn are respectively clade and nested clade distances (Templeton et al, 1995). Interior vs tip contrasts for Dc and Dn are indicated with ‘I–T’ in the corresponding clade. Superscript S and superscript L indicate that distance measures are significantly smaller and larger, respectively, than expected under random distribution of haplotypes.

0-step: 24-1 24-2 24-3 24-5 25-1 25-5 25-3 25-2 25-4 28-3 28-5 28-1 28-2 28-4

1-step: 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.10 1.11

2-step: 2.1 2.2 2.3 2.5

Dc: 0.0S 0.0S 0.0 0.0

Dn: 30.7L 35.4L 367.0 122.3

(I-T)c: 0.0 0.0

(I-T)n: 4.7L 244.7

3-step: 3.1 3.2

Dc: 32.8S 183.5

Dn: 179.4S 291.4L

(I-T)c: _

(I-T)n: _

c – Results of the NCA computed on Calobiusurbanelliae Ionian.Boxes reflect the nesting structure. Dc and Dn are respectively clade and nested clade distances (Templeton et al, 1995). Interior vs tip contrasts for Dc and Dn are indicated with ‘I–T’ in the corresponding clade. Superscript S and superscript L indicate that distance measures are significantly smaller and larger, respectively, than expected under random distribution of haplotypes.

0-step: 2.1 2.4 2.5 10.3 8.7 9.1 19-1 17-2 17-4 18-3 18-1 18-5 18-2 17-1 10-4 19-2

1-step: 1.1 1.2 1.3 1.4 1.6 1.12 1.13 1.14 1.16 1.23 1.31

2-step: 2.1 2.2 2.3 2.6 2.7 2.10 2.14

Dc: 10.9S 76.5S 40.0 44.0

Dn: 179.1 251.4L 35.6 31.7

(I-T)c: 65.6 -4.0

(I-T)n: 72.3 4.0

3-step: 3.1 3.2 3.3 3.4 3.6

Dc: 210.2 0.0S 39.3 0.0

Dn: 212.7 200.0 106.0 569.5

(I-T)c: -210.2S 39.3

(I-T)n: -13 -464.0

4-step: 4.1 4.2 4.3

Dc: 210.0S 157.1S 0.0

Dn: 316.2S 392.4L 344.0

(I-T)c: -42.2

(I-T)n: 74.8L

d– Results of the NCA computed on Calobiusurbanelliae –Tyrrhenian. Boxes reflect the nesting structure. Dc and Dn are respectively clade and nested clade distances (Templeton et al, 1995). Interior vs tip contrasts for Dc and Dn are indicated with ‘I–T’ in the corresponding clade. Superscript S and superscript L indicate that distance measures are significantly smaller and larger, respectively, than expected under random distribution of haplotypes.