Table S3Non-redundant proteins identified in Cyclamen persicum embryos
Protein name1 / Physiological pathway2 / Physiological function2 / ID31 / Alcohol dehydrogenase / glycolysis / a) carbohydrate metabolism / ID558, ID558
2 / Aldehyde dehydrogenase / glycolysis / a) carbohydrate metabolism / ID387
3 / Enolase / glycolysis / a) carbohydrate metabolism / ID387, ID638, ID983, ID351, ID1051, ID1043, ID799, ID353, ID863, ID8980, ID480, ID475, ID1038, ID641, ID1032, ID976, ID1020, ID1083, ID981, ID986, ID11657, ID831, ID1022, ID1033, ID17377, ID17302, ID997, ID803, ID18597, ID763
4 / Fructose-bisphosphate aldolase / glycolysis / a) carbohydrate metabolism / ID566, ID568, ID572, ID871, ID314
5 / Glyceraldehyde-3-phosphate dehydrogenase B,W / glycolysis / a) carbohydrate metabolism / ID1013, ID2424, ID714, ID757, ID602,ID2532,ID793,ID615,ID607,ID580,ID626,ID8929,ID199,ID682,ID600,ID18847,ID1025,ID17504,ID606,ID16803
6 / Malate dehydrogenase / glycolysis / a) carbohydrate metabolism / ID665,ID1387,ID648,ID702,ID637,ID1310
7 / Phosphoglycerate kinase / glycolysis / a) carbohydrate metabolism / ID9067,ID998,ID556,ID549,ID550
8 / Phosphoglyceromutase / glycolysis / a) carbohydrate metabolism / ID322
9 / Pyruvate dehydrogenase / glycolysis / a) carbohydrate metabolism / ID578
10 / Pyruvate kinaseB / glycolysis / a) carbohydrate metabolism / ID420
11 / Triosephosphate isomeraseB, W / glycolysis / a) carbohydrate metabolism / ID777, ID633, ID771
12 / UDP-glucose pyrophosphorylase / glycolysis / a) carbohydrate metabolism / ID441, ID381
13 / 6-phosphogluconate dehydrogenase / oxidative pentose phosphate pathway / a) carbohydrate metabolism / ID476
14 / UDP-glucose dehydrogenase 1 / oxidative pentose phosphate pathway / a) carbohydrate metabolism / ID411, ID445
15 / ATP synthase (complex v) subunit alpha / oxydative phosphorylation / b) energy metabolism / ID554
16 / ATPase, mitochondrial / oxydative phosphorylation / b) energy metabolism / ID438,ID1957,ID9406,ID12804,ID9601,ID1970,ID929,ID920
17 / Carbonic anhydrase / oxydative phosphorylation / b) energy metabolism / ID789
18 / NADH-ubiquinone oxidoreductase / oxydative phosphorylation / b) energy metabolism / ID2114
19 / 2-oxoacid dehydrogenase family protein / tricarboxylic acid cycle / b) energy metabolism / ID519
20 / Aconitase / tricarboxylic acid cycle / b) energy metabolism / ID160,ID4147,ID157,ID158
21 / Dihydrolipoamide S-acetyltransferase / tricarboxylic acid cycle / b) energy metabolism / ID8206
22 / Malic enzyme / tricarboxylic acid cycle / b) energy metabolism / ID308
23 / Succinate dehydrogenase / tricarboxylic acid cycle / b) energy metabolism / ID317
24 / Succinyl-CoA ligase / tricarboxylic acid cycle / b) energy metabolism / ID700, ID7552
25 / Aspartate aminotransferase / amino acid metabolism / c) other metabolic pathways / ID537, ID554
26 / Cysteine desulfurase / amino acid metabolism / c) other metabolic pathways / ID952, ID443
27 / Dihydroxy-acid dehydratase / amino acid metabolism / c) other metabolic pathways / ID369
28 / D-isomer specific 2-hydroxyacid dehydrogenase / amino acid metabolism / c) other metabolic pathways / ID368
29 / Peptidase / amino acid metabolism / c) other metabolic pathways / ID290, ID17559
30 / Peptidyl-prolyl cis-trans isomerase / amino acid metabolism / c) other metabolic pathways / ID941, ID942
31 / Serine hydroxymethyltransferase / amino acid metabolism / c) other metabolic pathways / ID444, ID462, ID944
32 / Seryl-tRNA synthetase / amino acid metabolism / c) other metabolic pathways / ID439
33 / Auxin-amidohydrolase / auxin action regulation / c) other metabolic pathways / ID853
34 / 3-oxoacyl-[acyl-carrier-protein] synthase I / lipid pathways / c) other metabolic pathways / ID516
35 / Acetyl-CoA carboxylase / lipid pathways / c) other metabolic pathways / ID452
36 / Enoyl-ACP reductase / lipid pathways / c) other metabolic pathways / ID677
37 / GDSL esterase/lipase / lipid pathways / c) other metabolic pathways / ID9002
38 / Glutamine synthetase / nitrogen fixation / c) other metabolic pathways / ID597
39 / Annexin / other processes / c) other metabolic pathways / ID653
40 / Beta-xylosidase/alpha-L-arabinosidase / other processes / c) other metabolic pathways / ID240
41 / Epimerase/Dehydratase, NAD-dependent / other processes / c) other metabolic pathways / ID782
42 / Formate dehydrogenase, mitochondrial / other processes / c) other metabolic pathways / ID574
43 / Glyoxalase I / other processes / c) other metabolic pathways / ID681
44 / Monodehydroascorbate reductase / other processes / c) other metabolic pathways / ID473
45 / Nucleoside diphosphate kinase / other processes / c) other metabolic pathways / ID1001, ID1024
46 / Oxidoreductase / other processes / c) other metabolic pathways / ID655
47 / Polygalacturonase / other processes / c) other metabolic pathways / ID377, ID3278, ID902
48 / Thiosulfate sulfurtransferase like protein / other processes / c) other metabolic pathways / ID884
49 / Cell division control protein / cell division and growth / d) cellular processes / ID139,ID146,ID16324,ID499
50 / Actin 1 / cytosceleton / d) cellular processes / ID3061,ID586,ID472,ID456
51 / Voltage-dependent anion channel / anion transport / e) transport / ID732, ID886
52 / 7S globulinW / globulin / f) storage proteins / ID8764
53 / SAL1 phosphatase / abscisic acid signaling pathway / g) environmental information processing / ID599
54 / Calmodulin / signal transduction / g) environmental information processing / ID938
55 / Aluminium induced protein / stress response / g) environmental information processing / ID825
56 / Anti-Oxidant 1 / stress response / g) environmental information processing / ID1074
57 / Apoptosis-related protein / stress response / g) environmental information processing / ID17455
58 / Catalase / stress response / g) environmental information processing / ID389,ID392,ID17829,ID386,ID388
59 / Copper/zinc superoxide dismutase / stress response / g) environmental information processing / ID767, ID1003,ID973
60 / Glutathione reductase / stress response / g) environmental information processing / ID414
61 / Manganese superoxide dismutase W / stress response / g) environmental information processing / ID858
62 / Osmotin-like protein / stress response / g) environmental information processing / ID3770,ID791
63 / Peroxiredoxin type II / stress response / g) environmental information processing / ID17171
64 / S-formylglutathione hydrolase / stress response / g) environmental information processing / ID1376
65 / Stress-inducible protein / stress response / g) environmental information processing / ID299
66 / Thioredoxin peroxidase 1 / stress response / g) environmental information processing / ID961
67 / Universal stress protein / stress response / g) environmental information processing / ID9796,ID962,ID9992,ID881
68 / DNA repair protein RAD23-4 / replication and repair / h) genetic information processing / ID9223
69 / Arginine/serine-rich splicing factor / transcription / h) genetic information processing / ID692
70 / Endoribonuclease / transcription / h) genetic information processing / ID1057
71 / Cysteine protease inhibitor / translation / h) genetic information processing / ID839
72 / Elongation factor 1-alpha / translation / h) genetic information processing / ID402,ID959,ID3864,ID336
73 / Elongation factor 2 / translation / h) genetic information processing / ID148
74 / Eukaryotic initiation factor 4A-like protein / translation / h) genetic information processing / ID1887
75 / Eukaryotic translation initiation factor 5A / translation / h) genetic information processing / ID16755
76 / Eukaryotic translation initiation factor iso4E / translation / h) genetic information processing / ID830
77 / Translation initiation factor / translation / h) genetic information processing / ID244
78 / Ubiquitin / translation / h) genetic information processing / ID1194, ID9986, ID810
79 / Aminopeptidase family protein / protein degradation / i) protein processing / ID413
80 / Protease / protein degradation / i) protein processing / ID548
81 / Proteasome / protein degradation / i) protein processing / ID878,ID801,ID412,ID3077,ID16796,ID804,ID852
82 / Chaperonin ClpB / protein folding / i) protein processing / ID124
83 / Heat shock protein 101 / protein folding / i) protein processing / ID787,ID108,ID2227,ID284
84 / Heat shock protein 20 / protein folding / i) protein processing / ID936,ID16827,ID985,ID880,ID947,ID879,ID911,ID897,ID919,ID898,ID1090,ID874
85 / Heat shock protein 60 / protein folding / i) protein processing / ID355,ID365,ID9070,ID216,ID215,ID340
86 / Heat shock protein 70 W / protein folding / i) protein processing / ID191,ID2478,ID15428,ID15879,ID14065,ID13817,ID3021,ID16112,ID190,ID7469,ID17688,ID761,ID844, ID750,ID270,ID766,ID850,ID632,ID7513,ID531,ID596
87 / Protein disulfide isomerase / protein modification / i) protein processing / ID612
88 / Em-like protein / embryogenesis/seed germination / j) development / ID1059
89 / LEA family protein / embryogenesis/seed germination / j) development / ID621
90 / SNF4 kinase-activating protein / embryogenesis/seed germination / j) development / ID573
uncharacterized / uncharacterized / k) uncharacterized / ID826,ID842,ID1014,ID9792, ID1060
1non-redundant proteins (sorted by proteine name) identified in the current study.
Protein name according to the best hit of MASCOT search against TAIR9, GreenPlant and NCBI databases.
Detailed information on mass spectrometry parameters for each spot analysed is given in Table S2 (supplementary material).
Bold printed proteins have been described previously by Bian et al. (2009; labelled with "B") or Winkelmann et al. (2006; labelled with "W").
2 functional protein classification according to KEGG PATHWAY Database.
3 spot ID represents the unique number of a protein spot in the 2D PAGEs and in the reference map.