Supplementary Table 1 Carcinoma Cell lines

Cell Line1 / Ploidy2 / Media3 / Supplier4 / Reference / SKY karyotype5 / 8 rearrangements by FISH6
Breast
BT-20 / 48 / DMEM/F12 / O’Hare / (Lasfargues et al, 1958) / Gr / ND
BT-474 / 103 / DMEM/F12 / O’Hare / (Lasfargues et al, 1978) / K / ND
BT-549 / 74-76 / RPMI-1640 / Clare / (Kytola et al, 2000) / K,R / ND
CaMa-1 / 78-79 / RPMI-1640 / Chaffanet / (Fogh et al, 1977) / K,P / ND
HCC1143 / 80 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1187 / 63 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1395 / 57 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1419 / 74 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1500 / 38 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1569 / 130 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1599 / 59 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1806 / 51 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1937 / 86 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC1954 / 90 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC38 / 73 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
HCC70 / 97 / FO / ATCC / (Gazdar et al, 1998) / Gr / ND
Hs578T / DMEM/F12 / O’Hare / (Hackett et al, 1977) / R / ND
MCF-7 / 65 / DMEM/F12 / ATCC / (Soule, et al. 1973) / D / 8q,8p
MDA-MB-134 / 44 / DMEM/F12 / O’Hare / (Cailleau, et al. 1978) / D / 8p
MDA-MB-157 / 62 / DMEM/F12 / O’Hare / (Young, et al. 1974) / D / 8q,8p
MDA-MB-175 / 48 / DMEM/F12 / O’Hare / (Cailleau et al, 1974) / D / 8p
MDA-MB-361 / 51 / DMEM/F12 / ATCC / (Satya-Prakash, et al. 1981) / D / 8q,8p
MDA-MB-415 / 80 / DMEM/F12 / O’Hare / (Cailleau et al, 1978) / Gr / ND
MDA-MB-453 / 90 / DMEM/F12 / ATCC / (Cailleau et al, 1978) / Gr / ND
PMC42 / 57 / DMEM/F12 / O’Hare / (Whitehead, et al. 1983) / D / 8q,8p
SK-BR-3 / 79 / DMEM/F12 / ATCC / (Fogh and Trempe 1975) / D / 8q,8p
SUM-1597 / 47 / FO / O / (Flanagan et al, 1999) / D / 8q only
SUM-447 / 49 / FO / O / (Mahacek et al, 1993) / P / ND
SUM-527 / FO / O / (Ethier et al, 1996) / Gr / ND
T47-D / 62 / DMEM/F12 / ATCC / (Keydar, et al. 1979) / D / 8p
UACC812 / 63-61 / DMEM/F12 / Sanger / (Meltzer et al, 1991) / K,P / ND
VP229 / 62 / FO / O / (McCallum et al, 1996) / D / ND
ZR-75-1 / 72 / DMEM/F12 / ATCC / (Engel et al, 1978) / D / 8p
Cervical
MT-1/hela / 103 / FO / O / (Hambly et al, 1997; MacLeod et al, 1999) / D / 8q,8p
SW9798 / 43 / RPMI-1640 / Lemoine / (Moore, et al. 2001) / S / 8p
Colorectal
C70 / 127 / GMEM / Bodmer / (Browning, et al. 1993) / A / 8q,8p
LS411 / 73 / GMEM / Bodmer / (Suardet, et al. 1992) / A / 8p
MT-39 / 46 / DMEM/F12 / O / (Hambly et al,1997) / D / None
SW1417 / 70 / DMEM/F12 / Bodmer / (Leibovitz, et al. 1976) / A / 8q,8p
SW403 / 64 / DMEM/F12 / Bodmer / (Leibovitz, et al. 1976) / A / 8q,8p
SW620 / 48 / DMEM/F12 / Bodmer / (Leibovitz, et al. 1976) / A / 8q,8p
SW837 / 38 / DMEM/F12 / Bodmer / (Leibovitz et al, 1976) / A / 8q,8p
VACO4A / 62 / DMEM/F12 / Bodmer / (Abdel-Rahman et al, 2001) / A / 8p
Melanoma
MDA-MB-43510 / 57 / DMEM/F12 / O’Hare / (Cailleau, et al. 1978; Ellison, et al. 2002) / D / 8q,8p
Pancreatic
CFPAC-1 / 62 / RPMI-1640 / Lemoine / (Schoumacher, et al. 1990) / S / 8p
Colo357 / 55 / RPMI-1640 / Lemoine / (Morgan, et al. 1980) / S / 8q,8p
PaCa-311 / 72 / RPMI-1640 / Lemoine / (Moore, et al. 2001) / S / 8p
Panc-1 / 59 / RPMI-1640 / Lemoine / (Lieber, et al. 1975) / S / 8p
PaTu-111,12 / 96 / RPMI-1640 / Lemoine / (Moore, et al. 2001) / S / 8p
RWP-1 / 59 / RPMI-1640 / Lemoine / (Dexter, et al. 1982) / S / 8q,8p
SUIT-2 / 81 / RPMI-1640 / Lemoine / (Iwamura, et al. 1987) / S / 8p

Other lines that had no rearrangements of 8 detected by our SKY analysis were Cal51, GI-101, SK-BR-7 (Breast); DLD1, GP2D, HCA7, LoVo, LS174T, VACO400, VACO429, VACO5 (Colorectal); A818.1, BxPC-3, MDA Panc-3 (Pancreatic)); lines with only rearrangements of 8q were HCT116, HT29, LIM1863 (Colorectal); AsPC-1, Hs766T, FA6 (Pancreatic). Lines that have chromosome 8 rearrangements but were not included in the present study: VP267 (Breast); Capan-1, SW480, MIA PaCa-2, PaTuII, QGP-1, Ro/Rossi, T3M-4 (Pancreatic); and HCT15 (Colorectal).

1All the lines listed were analysed by arrray-CGH, except Capan-2, SW979, SW1417, PaCa-3, and Panc-1.

2 Determined from the modal no. given in Davidson et al.,(2000), Abdel Rahman et al., (2001), Popovici et al., 2002, Kytola et al., 2000 or Sirivatanauksorn et al.,(2001), or unpublished

3 Media abbreviations: DMEM/F12 - Dulbecco's Modified Eagle Media:F12 nutrient mixture, GMEM - Glasgow Minimum Essential Media, RPMI-1640 - Roswell Park Memorial Institute Medium 1640, FO – as described by original reference.
4 Sources:

ATCC, American Type Culture Collection, in most cases via the European Collection of Animal Cell Cultures (ECACC, Porton Down, UK)

Bodmer, Sir Walter F Bodmer (CRUK Cancer and Immunogenetics Laboratory, Weatherall Institute of Molecular Medicine, Oxford, UK)

Chaffanet, Dr Max Chaffanet (Departement d’Oncologie Moleculaire, Institute Paoli Calmettes, Marseille)

Clare, Clare Hall Laboratories, Cancer Research UK, South Mimms, UK

Lemoine, Prof Nicholas R Lemoine (CRUK Molecular Pathology Laboratory, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, London ,UK)

O, from the originator

O’Hare, Prof Michael J. O'Hare (LICR/UCL Breast Cancer Laboratory, UniversityCollege, MiddlesexMedicalSchool, London, UK)

Sanger, from The Sanger Institute, Hinxton, UK

5 SKY karyotypes of the stocks used: D, Davidson et al., (2000); A, Abdel-Rahman et al., (2001); Gr Grigorova et al., (2004) and unpublished; S, Sirivatanauksorn et al., (2001). These are on our website SKY or M-FISH karyotypes obtained by others on different stocks: K, Kytola et al., (2000); P Popovici et al., (2002); R, Roschke et al., (2003).

6 Lines with chromosome 8 rearrangements analysed by metaphase FISH. Rearrangements of 8 detected by SKY were divided into rearrangements breaking within 8q or 8p by 8q arm painting: 8p, break within 8p; 8q, break within 8q. ND, FISH analysis not done; ND/NRG1, not done but NRG1 translocation analysed previously (Adelaide et al., 2003).

7 details of the SUM lines can be found at

8the ‘pancreatic’ cell line SW979 probably originated from a cervical carcinoma, since it has HPV and lacks typical pancreatic cancer mutations (Moore et al., 2001)

9 the ‘breast line’ MT3 was determined to be LS-174T, a colon cell line (

10 the ‘breast’ cell line MDA-MB-435 is now thought to have originated from a melanoma (Ellison et al., 2002)

11 derived by Dr M von Bülow (University of Mainz, Germany)

12 The cell line listed as PaTu-I from Sirivatanauksorn et al.(2001) was originally called Panc Tu-1 (Moore et al., 2001).

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