Supplementary Table.ORF predictionsa in the IIV25 genome. ORF in reverse orientation are highlighted in grey, non-coding segment (NCS) in yellow and pseudogenes in green.
ORF N° / Nucleotide positions in the genome / Peptidesize (amino acids) / Molecular Mass
(kDa) / pI / Putative Function / Closest viral or cellular orthologues
001R / 1>3699 / 1233 / 142.9 / 8.97 / DNA polymerase (B family) / CIV037L, MIV120R, WIV116R, DpAV4 ORF1
002R / 3770>4102 / 111 / 12.8 / 5.23 / Hypotheticalprotein / CIV 049L, WIV117R
003R / 4213>7089 / 959 / 111.5 / 6.86 / D5 familyhelicase-primase / CIV184R, , MIV121R, WIV120R, HvAV3e ORF119, TnAV2c ORF078,, SfAV1a ORF099, YP_001029423.1: GfV-C20-ORF1
004R / 7287>7775 / 163 / 18.4 / 4.53 / Hypotheticalprotein / WIV121R, AmEPV ORF075
005L / 8096>7812 / 95 / 10.7 / 6.16 / Hypotheticalprotein / WIV122L, MIV126R
006L / 9182>8322 / 287 / 32.5 / 5.35 / Hypotheticalprotein / WIV123L, MIV125R
007L / 13268>9297 / 1324 / 129.4 / 5.19 / Hypotheticalprotein / CIV261R, 396L, 443R, MIV091L, WIV124L
008L / 13987>13373 / 205 / 23.2 / 5.57 / Hypotheticalprotein / CIV056R, MIV124R, WIV125L
009R / 14006>14386 / 127 / 15.3 / 7.70 / Hypotheticalprotein / MIV123L, WIV126R
010L / 14597>14439 / 53 / 6.10 / 3.91 / Hypotheticalprotein / WIV127L
011R / 14624>14833 / 70 / 8.3 / 9.21 / Hypotheticalprotein / MIV117L, WIV128R
012R / 14962>17649 / 896 / 98.4 / 10.09 / Putative ubiquitinthioesteraseVirion core protein / CIV232R, TIV-VL96, MIV084L, WIV129R, DpAV4a-ORF008, IIV1 VL96
013R / 17696>18211 / 172 / 19.6 / 4.86 / Hypotheticalprotein / CIV358L, MIV083L, WIV130R
014R / 18369>19622 / 418 / 49.4 / 8.37 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112 , MSV-ORF021,198,199
015R / 19833>20267 / 145 / 17.1 / 9.33 / Hypotheticalprotein / MIV082L, WIV133R
016L / 20942>20475 / 156 / 18.6 / 9.34 / Ervl/Alrfamilyprotein / CIV347L, MIV096R, WIV134L, DpAV4a-ORF041, SfAV1a-ORF061, TnAV2c-ORF118
017R / 21028>22113 / 362 / 41.2 / 8.17 / matrix metalloproteinase-17-like / MIV095L, WIV135R, XP_001945941.2 [Acyrthosiphonpisum]
SNC / 22114>22515 / 401-bp SNC
018L / 23622>22516 / 369 / 37.6 / 4.76 / Hypotheticalprotein / CIV261R, 396L,443R, MIV091L, WIV136L
019L / 24452>23718 / 245 / 28.5 / 9.23 / Putative tyrosine phosphatase / CIV197R, MIV067L, WIV137L
020R / 24581>26101 / 507 / 58.7 / 6.56 / DNA repairexonucleaseSbcCD D subunit / CIV244L, MIV078R, WIV138R, DpAV4a-ORF026, MARV-ORF315, HvAV3e-ORF071, SfAV1a-ORF059, TnAV2c-ORF122
021L / 26572>26291 / 94 / 9.4 / 9.50 / Hypotheticalprotein / CIV368R, WIV103R
022L / 27156>26596 / 187 / 21.5 / 8.99 / Hypotheticalprotein / CIV234R, MIV073R, WIV140L
SNC / 27157>27970 / 813-bp SNC
023R / 27971>28432 / 154 / 17.5 / 4.46 / Hypotheticalprotein / MIV072L, WIV141R
024L / 29574>28480 / 365 / 43.3 / 7.60 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112 , MSV-ORF021,198,199
SNC / 29574>30025 / 451-bp SNC
025L / 33433>30026 / 1136 / 130.9 / 8.54 / Dynein-like beta chain / CIV295L, MIV016R, WIV143L, DpAV4a-ORF085, HvAV3e-ORF146, SfAV1a-ORF084, TnAV2c-ORF043, MARV-ORF306
026R / 33531>34844 / 438 / 52.0 / 8.09 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112 , MSV-ORF021,198,199
027R / 34955>36280 / 442 / 50.9 / 9.46 / Putative helicase / CIV161L, MIV109L, WIV145R
028R / 36378>37622 / 415 / 49.1 / 8.20 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112 , MSV-ORF021,198,199
029L / 38714>37665 / 350 / 41.3 / 9.39 / Hypotheticalprotein / WIV148L
030L / 39381>38752 / 210 / 24.6 / 7.71 / Hypotheticalprotein / CIV357R, MIV066L, WIV149L, DpAV4a-ORF097
031R / 39539>41866 / 776 / 90.3 / 5.72 / Hypotheticalprotein / CIV149L,155L, MIV113L, WIV150R, DpAV4a-ORF094
032R / 41879>42109 / 77 / 8.5 / 8.72 / immune reactive putative protease inhibitor / AF368912_1, [Glossina ,morsitans]
033L / 42464>42147 / 106 / 12.0 / 8.91 / Hypotheticalprotein / CIV466R, MIV112R, WIV151L
034L / 42976>42527 / 150 / 17.1 / 5.88 / hydrolase, NUDIX family / CIV414L, MIV111R, WIV152L,DpAV4a-ORF005, HvAV3e-ORF65bis, SfAV1A-ORF054, TnAV2c-ORF129
035R / 43094>43654 / 187 / 21.5 / 7.02 / Hypotheticalprotein / CIV395R, MIV001R, WIV153R
036L / 44356>43850 / 169 / 20.0 / 9.47 / RNA polymerase II subunit Rpb5b / MIV092R, WIV154L
037L / 44937>44392 / 182 / 21.7 / 7.66 / putativedeoxynucleotide monophosphate kinase / WIV155L, LauV ORF223
038L / 46132>44990 / 381 / 42.5 / 6.77 / Hypotheticalprotein / CIV228L, WIV156L
SNC / 46133>46416 / 283-bp SNC
039R / 46417>47109 / 231 / 25.2 / 7.06 / Hypotheticalprotein / CIV411L, MIV032R, WIV157R
040R / 47328>48119 / 264 / 30.9 / 5.45 / Hypotheticalprotein / WIV158R
041R / 48365>48889 / 175 / 19.5 / 6.28 / Hypotheticalprotein / CIV415R, MIV018L, WIV159R
042R / 49003>50106 / 368 / 42.5 / 5.75 / Flapendonuclease 1-A / CIV369L, MIV076L, WIV160R, Anoplopomafimbria - FEN1A
043L / 51489>50137 / 451 / 52.2 / 8.36 / BRO-like protein / WIV164L, MimiVgene L5,L12
044R / 51614>55651 / 1346 / 152.3 / 6.12 / DNA-directed RNA polymerase II largestsubunit / CIV176R, MIV090L, WIV165R, DpAV4a-ORF089, HvAV3e-ORF011, SfAV1a-ORF008, TnAV2c-ORF042
045L / 55954>55703 / 84 / 9.7 / 5.87 / Hypotheticalprotein / WIV166L
046L / 56151>55984 / 56 / 6.1 / 7.55 / Hypotheticalprotein / WIV167L
047L / 57954>56539 / 472 / 55.6 / 7.56 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112 , MSV-ORF021,198,199
048L / 58626>58039 / 196 / 23.1 / 9.50 / High mobility group proteinhomolog / CIV401R, MIV068R, WIV169L, DpAV4a-ORF022
049R / 58786>59268 / 161 / 18.1 / 8.13 / Dual specificityprotein phosphatase 3 / WIV170R, EFN81305.1, [Harpegnathossaltator]
050R / 59366>60070 / 235 / 26.5 / 6.32 / Hypotheticalprotein / MIV116R, WIV171R
051L / 60620>60141 / 160 / 17.6 / 8.52 / Hypotheticalprotein / MIV119R, WIV172L
SNC / 60621>60967 / 346-bp SNC
052L / 61222>60968 / 85 / 9.0 / 8.31 / Hypotheticalprotein / CIV115R, MIV031R, WIV174L
053R / 61345>61824 / 160 / 17.9 / 7.67 / Hypotheticalprotein / MIV114L, WIV175R
054R / 61900>62505 / 202 / 23.1 / 8.49 / Fasciclin-likeprotein / MIV081L, WIV176R
055R / 62601>64037 / 479 / 53.4 / 8.33 / Papain-likeproteinase / CIV361L, 224L, MIV024R, WIV177R, DpAV4a-ORF048
056R / 64100>64522 / 141 / 15.8 / 4.54 / Hypotheticalprotein / WIV178R
057R / 64522>64764 / 81 / 9.2 / 8.15 / Hypotheticalprotein / WIV179R
058L / 65368>64793 / 192 / 21.5 / 4.83 / Hypotheticalprotein / WIV180L, NP_932719.1, CfDNVORF110,, YP_003429353.1, PrGV ORF29
059R / 65508>66410 / 301 / 34.5 / 8.63 / Hypotheticalprotein / CIV355L, MIV017R, WIV181R
SNC / 66411>66747 / 336-bp SNC
060R / 66748>68034 / 429 / 50.4 / 7.68 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
061R / 68093>69070 / 326 / 35.6 / 5.75 / Hypotheticalprotein / CIV117L, MIV107R, WIV183R
SNC / 69071>69640 / 569-bp SNC
062L / 69928>69641 / 96 / 11.6 / 8.80 / Hypotheticalprotein / MIV023R, WIV184L
063R / 69991>70467 / 159 / 19.0 / 9.06 / Hypotheticalprotein / MIV050L, WIV185R
064L / 72602>70503 / 700 / 79.9 / 6.03 / Hypotheticalprotein / MIV049R, WIV186L
065R / 72707>73804 / 366 / 43.6 / 6.61 / Ribonucleoside-diphosphatereductase, beta subunit / CIV376L, , MIV048L, WIV187R, DpAV4a-ORF017,066
066R / 74032>74532 / 167 / 18.9 / 9.48 / Hypotheticalprotein / CIV111R, MIV025R, WIV188R
067R / 74586>75251 / 222 / 25.6 / 8.90 / Hypotheticalprotein / CIV350L, MIV026R, WIV189R,HvAV3e-ORF105, SfAV1a-ORF113, TnAV2c-ORF100
068R / 75336>75767 / 144 / 16.8 / 8.02 / Putative RING finger protein / CIV157L, MIV027R, WIV190R
069L / 76272>75862 / 137 / 15.3 / 8.27 / Hypotheticalprotein / WIV191L
070R / 76345>77526 / 394 / 45.1 / 9.61 / Hypotheticalprotein / CIV067R, MIV004R, WIV001R, DpAV4a-ORF064, MARV-ORF229
071R / 77547>77822 / 92 / 10.4 / 5.58 / Hypotheticalprotein / WIV002R
072R / 77914>78297 / 128 / 15.3 / 8.47 / Hypotheticalprotein / WIV003R
073L / 79250>78351 / 300 / 33.3 / 6.37 / Hypotheticalprotein / MIV005L, WIV004L
074R / 79416>80969 / 518 / 55.9 / 5.94 / Lipid membrane protein / CIV118L, 458R, MIV006R, WIV005R, DpAV4a-ORF005, 040
075R / 80985>81320 / 112 / 12.4 / 9.43 / Hypotheticalprotein / CIV248R, WIV007R
076R / 81391>82146 / 252 / 31.1 / 5.66 / Hypotheticalprotein / CIV404L, WIV008R
077L / 82646>82200 / 149 / 17.7 / 9.11 / Hypotheticalprotein / MIV015R, WIV009L
078R / 82857>84272 / 472 / 52.1 / 6.66 / Major Capsid Protein / CIV274L, MIV014L, WIV010R, DpAV4a-ORF019
SNC / 84273>84824 / 569-bp SNC
84825>86276 / 167 / 19.4 / 9.14 / Pseudogene ofzinc-ribbon-containing protein(Stop codon at 85326) / CIV273R,WIV012R, MarV ORF249, 301, 344 & 371, LauV ORF 265, 307,
079R / 86539>86799 / 77 / 10.5 / 7.95 / Hypotheticalprotein / MIV013L, WIV015R
080R / 86912>90133 / 1074 / 125.2 / 9.03 / Putative tyrosine protein kinase / CIV179R, MIV035R, WIV016R, DpAV4a-ORF105
081R / 90246>90518 / 91 / 10.6 / 5.44 / BRO-like gene / CIV201R, 289L, MIV019R, WIV087R, DpAV4a-ORF047,075
082R / 90490>90993 / 168 / 20.2 / 8.56 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
083R / 91008>91463 / 152 / 17.8 / 8.78 / Putative transcription elongation factor TFIIS / CIV349L, MIV055R, WIV017R, DpAV4a-ORF082, TnAV2c-ORF099
084L / 92902>91628 / 425 / 46.6 / 5.50 / Hypotheticalprotein / CIV198R, MIV069L, WIV019L
085R / 92968>93870 / 301 / 33.1 / 4.72 / Chitinase / WIV020R, ZP_04617184.1, [Yersinia ruckeri]
086R / 93946>94374 / 143 / 16.3 / 7.86 / Hypotheticalprotein / MIV057L, WIV021R
087R / 94503>95519 / 339 / 39.2 / 5.12 / Hypotheticalprotein / CIV287R, MIV056L, WIV022R,MARV-ORF339
088L / 97172>95583 / 530 / 60.1 / 9.23 / Serine/threonineprotein kinase / CIV380R, MIV010L, WIV023L
089R / 97282>97809 / 176 / 20.9 / 4.28 / Hypotheticalprotein / CIV293R, WIV024R
090R / 97928>99070 / 381 / 44.5 / 8.19 / Hypotheticalprotein / CIV302L, MIV012R, WIV025R
091R / 99165>100598 / 478 / 56.2 / 7.99 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
092L / 101130>100666 / 155 / 17.6 / 9.16 / ribonucleotide-diphosphatereductasesubunit alpha / CIV085L, MIV065R, WIV027L, DpAV4a-ORF054
093R / 101304>102200 / 299 / 33.6 / 4.38 / Hypotheticalprotein / MIV054L, WIV029R
094R / 102276>102629 / 118 / 13.6 / 9.66 / Hypotheticalprotein / MIV102R, WIV030R
SNC / 102630>102951 / 321-bp SNC
095R / 102952>104202 / 417 / 46.1 / 8.62 / putative myristylated membrane protein / CIV337L, MIV047R, WIV031R, DpAV4a-ORF065
SNC / 104203>104597 / 396-bp SNC
096R / 104598>106016 / 473 / 55.6 / 5.54 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
097L / 106768>106094 / 225 / 25.9 / 5.34 / Putative IAP / MIV021L, WIV032L
098L / 107442>106825 / 206 / 23.2 / 8.86 / Hypotheticalprotein / MIV022L, WIV033L
099L / 108405>107572 / 278 / 32.0 / 5.74 / RNAseIII / CIV142R, MIV101R, WIV034L, DpAV4a-ORF003
SNC / 108406>108827 / 421-bp SNC
100L / 109385>108828 / 186 / 22.2 / 6.66 / Haloaciddehalogenase-like hydrolases / CIV355R, MIV104L, WIV036L, DpAV4a-ORF117, HvAV3e-ORF110, SfAV1a-ORF109, TnAV2c-ORF093
101R / 109511>110239 / 243 / 28.2 / 8.39 / Hypotheticalprotein / MIV105R, WIV037R
102L / 111092>110292 / 267 / 26.8 / 4.47 / Hypotheticalprotein / CIV159L,261R, 396L, 443R, MIV091L, WIV067L
103L / 112704>111229 / 492 / 49.8 / 4.53 / Hypotheticalprotein / CIV159L,261R, 396L, 443R, MIV091L, WIV038L
104L / 114254>112782 / 491 / 49.4 / 4.60 / Hypotheticalprotein / CIV159L,261R, 396L, 443R, MIV091L, WIV039L
105R / 114393>115820 / 476 / 54.3 / 9.03 / ATP-dependent DNA helicase PIF1 / CIV030L, MIV106R, WIV040R
106R / 116020>116910 / 297 / 35.3 / 6.57 / Hypotheticalprotein / WIV041R
107R / 117000>117563 / 188 / 22.2 / 9;24 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
108L / 118212>117571 / 214 / 23.8 / 9.26 / Hypotheticalprotein / CIV259R, MIV071L, WIV042L, DpAV4a-ORF116
109R / 118576>119070 / 165 / 18.7 / 5.30 / Thioredoxin / CIV196R, MIV020R, WIV043R, Phage MedDCM-OCT-S09-C23-ORF196R,
MARV-ORF313
110L / 119781>119149 / 211 / 24.5 / 9.30 / RuvC-likeHollidayjunctionresolvase / CIV170L, MIV097L, WIV044L, DpAV4a-ORF039, MARV-ORF216, DpAV-ORF004
111R / 119916>120476 / 187 / 20.3 / 8.65 / dUTPase / WIV045R, MdSGHV ORF052
112R / 120589>121905 / 439 / 51.9 / 6.61 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
113R / 121949>122518 / 190 / 22.1 / 8.98 / dihydrofolatereductase / WIV069R, XP_001954901.1 [Drosophila ananassae]
114L / 122748>122488 / 87 / 9.9 / 6.54 / Hypotheticalprotein / WIV047L
115R / 122943>124646 / 568 / 65.6 / 8.54 / 5’-3’ Exoribonuclease 1 / CIV012L, MIV059L, WIV048R
116L / 124839>125324 / 162 / 18.3 / 4.35 / Hypotheticalprotein / WIV049R
117R / 125425>125934 / 170 / 19.9 / 4.79 / Hypotheticalprotein / CIV411L, MIV032R, WIV050R
118L / 126559>126128 / 144 / 16.9 / 4.33 / Hypotheticalprotein / CIV391R, MIV058R, WIV0561L, DpAV4a-ORF108
119L / 127068>126649 / 140 / 16.2 / 8.87 / Putative IAP / WIV052L, ACN11176.1 [Salmosalar]
120R / 127269>128033 / 255 / 29.8 / 6.26 / Proliferatingcellnuclearantigen / CIV436R, MIV060L, WIV053R DpAV4a-ORF025, MARV-ORF212
121R / 128081>129394 / 438 / 52.1 / 8.13 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
122L / 132376>129443 / 978 / 113.5 / 8.54 / SWI/SNF2 familyhelicase / CIV022L, MIV087L, WIV055L, DpAV4a-ORF020
123R / 132586>133335 / 250 / 29.0 / 9.51 / SWIB complex BAF60b domain-containingprotein / CIV306R, MIV070L, WIV056R
124L / 134977>133388 / 530 / 62.3 / 8.59 / Tyrosine kinase / CIV439L, MIV098L, WIV058L
125R / 135078>136433 / 452 / 53.2 / 8.83 / Immediateearlyprotein ICP-46 / CIV393L, MIV039R, WIV059R, DpAV4a-ORF043, HvAV3e-ORF078, SfAV1a-ORF065, TnAV2c-ORF113
126R / 136649>137035 / 129 / 15.0 / 6.83 / Ribonuclease III / CIV340R, WIV060R
127R / 137337>137693 / 119 / 13.4 / 8.64 / Thiredoxin-like / CIV453L, MIV041R, WIV062R, DpAV4a-ORF044
128L / 138687>138052 / 212 / 23.6 / 4.79 / Hypotheticalprotein / WIV064L
129R / 138753>140405 / 551 / 64.6 / 5.80 / Serine/threonineprotein kinase / CIV098R, MIV038R, WIV065R, DpAV4a-ORF036, HvAV3e-ORF117, SfAV1a-ORF104, TnAV2c-ORF088
SNC / 140406>140741 / 335-bp SNC
130L / 146885>140742 / 2048 / 203.0 / 4.93 / Hypotheticalprotein / CIV261R, 396L,443R, MIV091L, WIV068L
131R / 147037>149136 / 700 / 81.6 / 5.23 / Hypotheticalprotein / CIV268L, MIV074L, WIV069R, DpAV4a-ORF079
132R / 149176>149865 / 230 / 26.9 / 7.50 / Hypotheticalprotein / -
133R / 149976>152342 / 789 / 90.1 / 6.77 / ribonucleotide-diphosphatereductasesubunit alpha / CIV085L, MIV065R, WIV070R, DpAV4a-ORF054
134R / 152466>153797 / 444 / 52.3 / 9.06 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
135L / 154837>153914 / 308 / 31.9 / 5.00 / Hypotheticalprotein / CIV261R, 396R & 443R, MIV091L, WIV072L
136L / 155402>154932 / 157 / 18.2 / 8.27 / Metallopeptidase WLM / CIV136R, MIV042R, WIV073L, MARV-ORF278, MIMIV-325R
137L / 156610>155429 / 394 / 46.7 / 8.82 / DNA binding/packingprotein / CIV282R, MIV079L, WIV074L, DpAV4a-ORF103, HvAV3e-ORF033, SfAV1a-ORF029, TnAV2c-ORF017
138R / 156775>157491 / 239 / 28.1 / 9.35 / Hypotheticalprotein / MIV080R, WIV075R
139R / 157718>158857 / 380 / 45.2 / 8.72 / Hypotheticalprotein / CIV198R, MIV069L, WIV076R
SNC / 158858>159384 / 526-bp SNC
140R / 159385>160140 / 252 / 28.9 / 7.65 / ATPase 3 / CIV075L, MIV088R,WIV079R, DpAV4a-ORF086, HvAV3e-ORF109, SfAV1a-ORF110, TnAV2c-ORF095
141L / 160733>160200 / 178 / 20.2 / 9.32 / histone H4-like / WIV080L, EFQ30757.1, [Glomerellagraminicola]
142R / 160815>161189 / 125 / 14.5 / 9.84 / GIY-YIG domain protein / WIV081R, ZP_06356040.2 [Citrobacteryoungae]
143L / 162194>161235 / 320 / 37.7 / 5.79 / serine/threonine-protein kinase pim-2-like / MIV044L, WIV082L
144R / 162432>162713 / 94 / 11.0 / 9.24 / Hypotheticalprotein / MIV045R, WIV083R
145R / 162844>164118 / 425 / 48.5 / 5.95 / Hypotheticalprotein / CIV229L, MIV046R,WIV084R
146L / 165119>164166 / 318 / 38.3 / 5.91 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
SNC / 165120>165664 / 544-bp SNC
147R / 165665>166387 / 241 / 27.0 / 11.37 / Hypotheticalprotein / CIV329R, MIV099R, WIV085R
148L / 167443>166454 / 330 / 36.3 / 9.92 / Hypotheticalprotein / CIV329R, MIV099R, WIV086L
149L / 169973>167490 / 828 / 87.2 / 5.05 / Hypotheticalprotein / CIV261R, 396L,443R, MIV091L, WIV124L
150L / 170227>170042 / 62 / 7.4 / 9.43 / Hypotheticalprotein / WIV088L
151R / 170648>174055 / 1136 / 129.8 / 8.93 / DNA topoisomerase II / CIV045L, MIV086L, WIV089R
152R / 174048>174710 / 221 / 26.0 / 9.32 / Hypotheticalprotein / MIV064L, WIV090R
153R / 174789>176033 / 415 / 49.4 / 8.21 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
154L / 176761>176072 / 230 / 26.8 / 6.21 / Hypotheticalprotein / CIV309L, MIV063R, WIV091L
155R / 176906>178366 / 487 / 57.5 / 8.67 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
156L / 180168>178705 / 488 / 56.7 / 6.19 / Hypotheticalprotein / CIV467R, MIV061R, WIV094L, MARV-ORF200
157R / 180251>181141 / 297 / 34.3 / 6.49 / Thymidylate synthase / WIV095R, NP_001040281.1 [Bombyxmori]
158R / 181229>182116 / 296 / 34.7 / 9.18 / Hypotheticalprotein / MIV028R, WIV097R
159R / 182167>182736 / 190 / 22.4 / 5.78 / Thymidine kinase / CIV143R, MIV029R, WIV098R, DpAV4a-ORF055, HvAV3e-ORF055, SfAV1a-ORF040, TnAV2c-ORF154
160L / 183185>182781 / 135 / 15.1 / 4.40 / Hypotheticalprotein / MIV030L, WIV099L
161R / 183243>183731 / 163 / 18.8 / 8.78 / Hypotheticalprotein / CIV115R, MIV031R, WIV100R
162R / 183924>184682 / 253 / 28.2 / 8.15 / Hypotheticalprotein / CIV411L, MIV032R, WIV101R
163R / 184751>185122 / 124 / 14.1 / 8.53 / Putative phosphatase / WIV102R, XP_003212936.1, [Meleagrisgallopavo],
164R / 185269>186546 / 426 / 50.9 / 8.90 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
165L / 186993>186595 / 133 / 15.7 / 8.74 / Uvr/REP helicase / CIV307L, MIV033L, WIV104L, DpAV4a-ORF028, HvAV3e-ORF144, SfAV1a-ORF086
166R / 187583>188410 / 276 / 31.1 / 9.20 / Hypotheticalprotein / CIV077L, MIV034R, WIV105R, DpAV4a-ORF060
167R / 188525>190969 / 815 / 95.3 / 8.22 / DNA double-strand break repair rad50 ATPase-like / CIV050L, MIV094L, WIV106R, DpAV4a-ORF033, MARV-ORF118, TnAV2c-ORF077
168R / 191162>191596 / 145 / 16.7 / 8.99 / DNA-directed RNA polymerase II subunit E / MIV053L, WIV107R
169L / 191884>191642 / 81 / 9.1 / 6.08 / Hypotheticalprotein / WIV108L
170R / 191976>193898 / 641 / 72.199 / 8.82 / NAD-dependent DNA ligase / CIV205R, MIV052L, WIV109R, MIMIV-303R
171R / 193981>195255 / 425 / 49.7 / 6.61 / Hypotheticalprotein / MIV007R, WIV110R
172R / 195336>196664 / 443 / 52.6 / 8.17 / Hypotheticalprotein / CIV006L, 019R, 029R,, 146R, 148R, 211L, 212L, 238R, 313L, 388R, 420R, 468L, WIV026R, 035l, 046R, 063R, 071R, 096R, 144R, 168R, DpAV4a-ORF004,016,032,, 034,050,067,098,110,112, MSV-ORF021,198,199
173L / 196935>196708 / 76 / 8.6 / 10.53 / Hypotheticalprotein / WIV111L
174L / 198815>196950 / 562 / 56.8 / 5.25 / Hypotheticalprotein / CIV261R, 396L,443R, MIV091L, WIV112L
175R / 198909>202301 / 1131 / 127.8 / 8.12 / DNA-directed RNA polymerase II subunit RPB2 / CIV428L, MIV009R, WIV113R, DpAV4a-ORF073
176R / 202372>202986 / 205 / 24.8 / 5.26 / Hypotheticalprotein / CIV404L, WIV114R
177L / 204648>203119 / 510 / 56;6 / 5.41 / Hypotheticalprotein / CIV213R, MIV051L, WIV115L
a, Methods: Genomic DNA composition, structure, and codon usage were analyzed using several desktop and online programs (DNASTAR (Lasergene); Nucleotide and protein sequence database searches were performed using the BLAST programs at NCBI website ( For more refined analyses, conserved motifs and domains, and putative functions of deduced DpAV4a proteins composed of 49 or more amino acids with homology to other proteins in sequence databases were identified using several online programs, as follows:
Physicochemical properties of the proteins: and
Conserved motifs and domains:
Secretion and maturation motif predictions: Non-classical and leaderless secretion of proteins: Twin-arginine signal peptides: Peptide signal: Transmembrane domains: TMHMM2.0 –
Dimerization motifs: WD domain repeat:
LeucineZippers:
DNA binding motifs: Zinc Finger domain: and Helix turn helix motif:
Post-translational modification : Kinase specific eukaryotic protein phosphorylation sites - and
Cellular location: Subcellular location of proteins: mitochondrial, chloroplastic, secretory pathway, or other:
Cellular trafficking: Nuclear localization signals: and Leucine-rich nuclear export signals (NES) in eukaryotic proteins:
Disulfidebridge:
Virus abbreviations used for non-iridovirus and ascovirus proteins are: AmEPV:Amsactamooreientomopoxvirus, BmNPV: Bombyxmorinucleopolyhedrosis virus, CfDNV: Choristoneurafumiferanadensovirus, CoGV: Choristoneuraoccidentalisgranulovirus, EpNPV: Epiphyaspostvittana NPV, GfV: Glyptafumiferanaeichnovirus, LdNPV: Lymantriadisparnucleopolyhedrosis virus, LauV: Lausannevirus, MarV: Marseillevirus, MIMI: AcanthamoebacastellaniiMimivirus, MSV: Melanoplussanguinipesentomopoxvirus, PrGV: Pierisrapaegranulaovirus, XcGV: Xestia c-nigrumgranulovirus.
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IIV25