OIE RL for « Foot and mouth disease » – « Dr. Wilai Linchongsubongkoch » – « Thailand »
OIE Reference Laboratory Reports
Activities in 2012
Address of laboratory / Regional Reference Laboratory for FMD in South East Asia,
National Institute of Animal Health
Department of Livestock Development,
Pakchong, Nakhonratchasima, 30130
THAILAND
Tel.: / +66 44 279112, +66 44 313869
Fax: / +66 44 314889, +66 44 313869
e-mail address: /
website: / www. dld.go.th/rrl
Name (including Title) of Head of Laboratory (Responsible Official): / Dr. Panithan Thongtha
Acting Director, Regional Reference Laboratory for FMD in South East Asia,
since 1st October 2011-12 July 2012.
Dr. Somjai Kamolsiripichaiporn
Acting Director, Regional Reference Laboratory for FMD in South East Asia,
since 13 July 2012 to the present
Name(including Title and Position) of OIE Reference Expert: / Dr. Wilai Linchongsubongkoch
Veterinary Expert and Consultant of Department of Livestock Development,
Date of submission to the OIE / 21 January 2012
Instructions
This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.
Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:
http://www.oie.int/en/our-scientific-expertise/reference-laboratories/introduction/
Each ToR (blueitalicised text) has been placed as a heading covering the group of questions related to it.
Please note the reditalicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.
The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.
This annual report will remain available for consultation on the OIE web site:
(http://www.oie.int/en/our-scientific-expertise/reference-laboratories/annual-reports/):
ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards
Test recommended by the OIE / Total number of test performed last yearIndirect diagnostic tests / Nationally / Internationally
Liquid phase blocking ELISA (LP ELISA) / 9,840 tests / 60 tests
Indirect blocking ELISA for 3ABC non structural protein (3ABC NSP) / 5,966 / 60
r-value or vaccine matching by LP ELISA / 16 / 2
Direct diagnostic test / Nationally / Internationally
Antigen capture ELISA / 70 tests / 27 tests
BHK-21 cell culture
/ 406 / 15Real time RT-PCR / 107 / 5
RT-PCR / 119 / 10
Sequencing
/ 39 / 5ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.
Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.
2. Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?
R Yes No
3. Did your laboratory supply standard reference reagents to OIE Member Countries?
R Yes No
The diagnostic reagents produced at Regional Reference Laboratory for FMD in South East Asia (RRLSEA), Thailand in 2012 mainly was ELISA reagents, such as rabbit trapping antibody, guinea pig detecting and inactivated concentrated antigen for FMD serotype O, A and Asia1, details as shown in table.
Type of reagent available / Related diagnostic test / Produced/stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions /
- Rabbit trapping antibody
- Type O
- Type A
- Type Asia1 /
LP ELISA ,
- Antigen capture ELISA
- and r-value by LP ELISA / Produced / 18 sets
22 sets
16 sets / 2 sets
2 sets
2 sets / Myanmar
Myanmar
Myanmar
- Guinea pig detecting antibody
- Type O
- Type A
- Type Asia1 / Produced / 19 sets
25 sets
17 sets / 2 sets
2 sets
2 sets / Myanmar
Myanmar
Myanmar
- Inactivated FMDV Concentrate antigen
- Type O
- Type A
- Type Asia1 / Produced / 72.5 ml
86.5 ml
93 ml / 8 ml
6 ml
6 ml / Myanmar
Myanmar
Myanmar
- Control serum
- C++(Strong)
- C+ (Moderate)
- C- (Negative) / Produced / 120 ml
105 ml
105 ml / 15 ml
15 ml
15 ml / Myanmar
Myanmar
Myanmar
4. Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference
reagents?
R Yes No
5. Did your laboratory produce vaccines?
Yes R No
6. Did your laboratory supply vaccines to OIE Member Countries?
Yes R No
ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases
7. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?
R Yes No
8. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?
R Yes No
Name of the new test or diagnostic method or vaccine developed / Description and References (Publication, website, etc.)1. Developed new technique on FMD
profiling test / Boonsuya Seeyo et al. (2012), FMD antigenic profiling ELISA. Proceedings on Thailand-Japan Joint Conference on Animal Health. 25th Anniversary of NIAH. 91-95
2. Developed new vaccine of FMD type A from local isolated virus, under the collaboration with the Vaccine Production Unit of Bureau of Veterinary Biologics , Thailand / Petvanichakul et al. (2012), Development of FMD type A/Lopburi/2012 new strain, on process of submission
to publish in Thai Vet, Journal
3. Validation of new diagnostic technique for detection of antibody to new developed 3ABC non structural protein ELISA for differentiate infected from vaccinated animals, under the research collaboration with Kasetsart University,
Bangkok, Thailand / Lekcharoensuk et al. (2012). Development of an inactivated 3Cpro-3ABC (mu3ABC) ELISA to differentiate cattle infected with foot and mouth disease virus from vaccinated cattle. This paper has been accepted and will be published soon in J. Virol. Methods.
.
ToR: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries
9. Did your laboratory carry out diagnostic testing for other OIE Member Countries?
R Yes No
Name of OIE Member Country seeking assistance / Date(dd/mm) / No. samples received for provision of diagnostic support (i.e. from surveillance campaign) / No. samples received for provision of confirmatory diagnoses
Cambodia / 24th September / 5 samples / -
Lao PDR / 28th December / 60 samples / 22 samples
10. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?
R Yes No
Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was providedCambodia and Lao PDR / Consultation on FMD diagnostic method, some trouble shooting on assay procedure / Giving advice and consultation by Email communication
Myanmar / Foot and mouth disease diagnosis / Lecture
Singapore / Foot and mouth disease diagnosis / Lecture and workshop
ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations
11. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?
R Yes No
Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your countryFoot and Mouth Disease Risk Management for Australia and South East Asia / 5years / To test the vaccine efficacy against FMD SEA strains , to enhance the existing laboratory capability, introduce new technology in molecular technique, and to maintain the biocontainment and microsecurity system checking by expert. / Australian Animal Health Laboratory
(AAHL), Australia, / Australia,
Argentina,
South Africa,
Vietnam
ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases
12. Did your Laboratory collect epizootiological data relevant to international disease control?
R Yes No
13. Did your laboratory disseminate epizootiological data that had been processed and analysed?
R Yes No
14. What method of dissemination of information is most often used by your laboratory?
(Indicate in the appropriate box the number by category)
a) Articles published in peer-reviewed journals: 10
b) International conferences: 6
c) National conferences: 1
d) Other: Internet: e-Journal -NIAH 2
List of publications as following.
a) Articles published in peer-reviewed journals
1. Linchongsubongkoch,W. was requested from OIE to review scientific paper for publishing in
OIE journal entitled “Ready to use multiples PCR kit for rapid detection of foot and mouth
disease virus serotypes in India”. Authorsby Sharma , G.K., Mahajan, S., Das, B., Ranjan, R.,
Kanani, A., Sanyal, A., Pattnaik,B. FMD Network Unit, Ahmadabad, Gujarat, India
2. Linchongsubongkoch,W., Boonsuya Seeyo,K., Samanit, J. 2012. Update on antigenic
variation of foot and mouth disease virus in South East Asia. Proceedings on the 37th ICVS 2012
& IICAB, APHIS, FAO Joint Symposiums . 383-387.
3. Srimahasombat, V., Tungthumniyom, N., Linchongsubongkoch, W., Sariya, L., Lekcharoensuk, C.,
Ramasoota, P., Lekcharoensuk, P. 2012. Development of recombinant m3ABC based ELISA to
differentiate infected from infected animals. Proceedings on the 37th ICVS 2012 & IICAB,
APHIS, FAO Joint Symposiums .
4. Boonsuya Seeyo, K., Linchongsubongkoch, W., Samanit, J. 2012. FMD antigenic profiling ELISA.
Proceedings on Thailand-Japan Joint Conference on Animal Health. 91-95
5. Linchongsubongkoch, W., Boonsuya Seeyo, K., Petvanichakul, S., Samanit, J. 2012. Vaccine
matching strain characterization of foot and mouth disease virus in South East Asia during 2010-
1012. Proceedings on Thailand-Japan Joint Conference on Animal Health. 47-51.
6. Nuanualsuwan, S., Youeimyut, P., Udon, R., Thongtha, P. 2012. Thermal inactivated of FMDV
serotype O in pork products. Proceeding on FAO/OIE Global Conference on foot and mouth
disease control. Page 79
7. Yano,T., Suphavilai, C., Wongsathapornchai, K., Linchongsubongkoch,W., Aumpomma, D.,
Thongtha, P., Rojanasthien, S., Kankeaw, N. 2012. Humeral and cell- mediated immune
response against national infection to foot and mouth disease virus in previously vaccinated
dairy cattle. Proceedings on FAO/OIE Global Conference on foot and mouth disease control.
Page 5.
8. Colling, A., Morrissy, C., Barr, J., Meehan, G., Wright, L., Goff, W., Gleeson, L.J., Golebiowski, B.,
Riddell, S., Yu, M., Eagles, D., Doughty, W., Khounsy, S., Thanlong, N., Vu, P. P., Phuong, N.T.,
Tung, N., Linchongsubongkoch, W., Hammond, J., Johnson, M., Unger, H., Crowther, J. 2012.
Development and validation of 3ABC FMD antibody ELISA. New Zealand Vet. Journal.
9. Petvanichakul, S., Sripisuth,S., Boonsuya Seeyo, K., Linchongsubongkoch, W. Development of
FMD type A/Lopburi/2012 new strain. on process of submission to publish in Thai Vet journal.
10. Linchongsubongkoch, W., Abila, R., Thongthga, P. Molecular epidemiology of foot and mouth
Disease in Southeast Asia. on process of submission to publish in international journal.
b) International conferences
1. Linchongsubogkoch, W. was invited as speaker to give presentation in the The 37th ICVS 2012 IICAB, APHIS, FAO Joint Symposiums, Bangkok, Thailand , 29 Feb -1st March 2012, entitled Update antigenic variation of FMDV in Southeast Asia.
2. Linchongsubogkoch, W. was invited to participating in the Global FMD Research Alliance (GFRA) Workshop , Surveillance, Epidemiology, Vaccine and Control of Foot and Mouth Disease, at Kruger National Park, South Africa during 17-19 April 2012. And was invited to give two presentations on title (1) Vaccine matching in Southeast Asia and (2) Epidemiolgy of Foot and Mouth Disease in Southeast Asia.
3. Linchongsubogkoch, W. was invited to attend and give presentation on Vaccine matching strain characterization of FMDV in Southeast Asia, Thailand-Japan Joint Conference on Animal Health 2012: The 25th Year Anniversary of National Institute of Animal Health, Bangkok, Thailand,30-31 May 2012.
4. Boonsuya Seeyo, K. and Samanit, J. was invited to attend and give presentation on FMD antigenic profiling ELISA, Thailand-Joint Conference on Animal health 2012: The 25th Year Anniversary of National Institute of Animal Health, Bangkok, Thailand, 30-31 May 2012.
5. Linchongsubogkoch, W. was attending the second OIE/FAO Global Conference on foot and mouth disease control, Bangkok, Thailand, 27-29 June 2012.
6. Kamolsiripichaiporn, S, Udon, R. Thaongtha, P and Boonsuya Seeyo, K. was attending the second OIE/FAO Global Conference on foot and mouth disease control, Bangkok, Thailand, 27-29 June 2012.
c) National conferences
1. Linchongsubogkoch, W. was invited as technical committee group of National Bureau of Agriculture Commodity and Food Standard (ACFS), Thailand to revision the national standard of diagnostic manual on foot and mouth disease, 6 February 2012
ToR: To provide scientific and technical training for personnel from OIE Member Countries
To recommend the prescribed and alternative tests or vaccines as OIE Standards
15. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?
R Yes No
a) Technical visits: 4
b) Seminars: N/A
c) Hands-on training courses: 1
d) Internships (>1 month): N/A
Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding countrya / Myanmar / 5
a / Korea / 6
a / Argentina / 7
a / National focal group in South East Asia / 20
c / Singapore / 2
ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned
16. Does your laboratory have a Quality Management System certified according to an International Standard?
R Yes No
Quality management system adoptedISO 17025:2005
17. Is your laboratory accredited by an international accreditation body?
R Yes No
Test for which your laboratory is accredited / Accreditation bodyAntigen typing ELISA / ILAC –MRA by Bureau of Laboratory Quality Standard, Dept. of Medical Science
Liquid phase blocking ELISA (LP ELISA) / ILAC -MRA by Bureau of Laboratory Quality Standard, Dept. of Medical Science
3ABC NSP blocking ELISA / ILAC -MRA by Bureau of Laboratory Quality Standard, Dept. of Medical Science
3 B NSP indirect ELISA / ILAC -MRA by Bureau of Laboratory Quality Standard, Dept. of Medical Science
18. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)