Isolation of Chemical Constituents from Roots of Convolvuluspluricaulis(Convolvulaceae)

“ISOLATION OF CHEMICAL CONSTITUENTS FROM ROOTS OF CONVOLVULUSPLURICAULIS(CONVOLVULACEAE) TOWARDS ACHIEVING CHEMICAL STANDARDISATION”

SYNOPSIS FOR

M.PHARM DISSERTATION

SUBMITTED TO

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES

KARNATAKA

NARAYANA KUMAR L.G.

I M.PHARM

DEPARTMENT OF PHARMACOGNOSY

PESCOLLEGE OF PHARMACY

BENGALURU-560050

(2010-11)

RAJIVGANDHIUNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BENGALURU.

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. /

Name of the candidate and address

/ NARAYANA KUMAR .L.G.
p.e.s.college of pharmacy,
HANUMANTHANAGAR,
50 FT. ROAD,
BENGALURU-560 050.

PERMANENT ADDRESS

S/O GOVINDAGOWDA
LAKKANAHALLY(AT AND POST)
SIRA TALUK
TUMKUR DISTRICT-572113.
2. / Name of the institution /

P.E.S.COLLEGE OF PHARMACY

HANUMANTHANAGAR,
50 FT. ROAD,
BENGALURU -560 050.
3. / Course of study and subject /

MASTER OF PHARMACY

IN

PHARMACOGnosY

4. / Date of the admission / 16thJUNE 2009
5. / Name of the topic
“ISOLATION OF CHEMICAL CONSTITUENTS FROM ROOTS OF CONVOLVULUSPLURICAULIS (CONVOLVULACEAE) TOWARDS ACHIEVING CHEMICAL STANDARDISATION ”
6. /

Brief resume of the intended work:

6.1 Need for the study:
Anherbal medicine is a medicinal preparation made from a plant, itcan include the fresh or dried herb, or an advanced form of the herb usually made via extraction by a solvent such as water or an organic solvent. Such advanced herbal preparations, often processed in a way that establishes relatively fixed parameters, are often called standardized extracts.However scientific data on their efficacy, as well as on their chemical constituents have so far been lacking. Here in our work we recognize the importance of using establishes modern scientific methods and criteria to characterize the compoundsfromConvolvulus pluricaulis.
Convolvulus pluricaulis (Convolvulaceae) is an indigenous plant commonly mentioned in Ayurveda an ancient system of Indianmedicine as a Rasayana which is mainly advocated for use in mental stimulation and rejuvenation therapy. The whole herb is used medicinally in the form of decoction with cumin and milk in fever, nervous debility, loss of memory, also in syphilis, and scrofula. Shankhapushpi is used as a brain tonic, febrifuge, in bowel complaints especially dysentery. The plant is reported to be a prominent memory improving drug. It is used as a psycho stimulant and tranquilizer.1
6.2 REVIEW OF LITERATURE:
Habitat:
Convolvulus pluricaulisgrows throughout India.
Synonyms ofConvolvulus pluricaulis.
English name-Bindweed.
Hindi name-Shankpushpi.
Sanskrit name-Vishnukranthi.
Kannada-Kadvalmar
Tamil-Vishnukrandi
Marati-Shankhavalli
BOTANICAL CLASSIFICATION:
Kingdom : Plantae
Division : Magnoliophyta
Class : Liliospida
Order : Urticalases
Family : Convolvulaceae
Chemical constituents:
Convolvulus pluricaulis contains flavonoids and coumarin derivatives.Flavonoids include kaempferol-3-glucosides, 6-methoxy-7- hydroxycoumarin has been reported from the plant.
Various long chain fatty alcohols such as n-hexacosanol, n-octacosanol, n-triaconatanol, and dottriacontanol are also present.It also contains Shankapuspine, 3, 4-dihydroxycinnamic acid, ß-sitosterols, sugars like glucose, rhamnose, sucrose.1,2
Uses:1,3

All parts of the plants are used
In the treatment of Haematemesis, Piles and incase of Constipation.
This plant is used in the treatment of Insomnia, chronic cough and Asthma.
Used in Seminal debility and as a Hypotensive.
Fresh juice used as a Nervine tonic in case of Epilepsy, Insanity and Nervous debility.

6.3Literature:

Neuroprotective role ofConvolvulus pluricaulis on aluminiminduced neurotoxicity in rat

brain was carried out by Bihaqi SW, et.al. Results of Convolvulus pluricaulis possesses

Neuroprotectivepotential, thus validating its use in alleviating toxic effects of alminium4.

Neuronutrient impact of Ayurvedic Rasayana therapy in brain aging has been carried out by
Singh RH, et.al.The scientific parameters have shown promising results which have been
received for further studies of the plant Convolvulus pluricaulis.5

Evaluation of the antidepressant –like activity of Convolvulus pluricaulischoisy in the

mouse forced swim and tail suspension tests was carried out by Dhingra D, et.al.

The results suggest that the chloroform fraction of the total ethanolic extract of

Convolvulus pluricauliselicited a significant antidepressant-like effect in mice by

interaction with the adrenergic, dopaminergic, and serotonergic systems.6

Kumar V. had review few Potential medicinal plants for CNS disorders he has listed out

Convolvulus pluricaulis is widely used for their reputed effectiveness in CNS disorders.7

Effect of Convolvulus pluricaulis on gastric ulceration and secretion in rats is performed
by Sairam K, et.al. Result suggests that the antiulcerogenic effect of CPJ was found to be
due to augmentation of mucosal defensive factors like mucin secretion.8
Inhibition of T3 production in levothyroxine-treated female mice by the root extract of
Convolvulus pluricauliswas studied by ByPanda S and Kar A. the study showed the
action of the plant extract on thyroid function is primarily mediated through the inhibition
of 5`-DI enzyme activity.9
Studies on medicinal properties of Convolvulus pluricaulis and Boerhaavia diffusa.
By Mudgal V. They have been studied the medicinal properties of these plants.10
Gas chromatographic identification of fatty acids, fatty alcohols, and hydrocarbons of
Convolvulus pluricaulis studied by Srivastava DN and Deshpande SM. They have been
identified fatty acids, fatty alcohols, and hydrocarbons by Gas chromatographic
technique.11
Preliminary pharmacological studies on Convolvulus pluricaulischoisan Indian indigenous
herb. Was carried out by Barar FS and Sharma VN. and they found out pharmacological
actions of Convolvulus pluricaulis.12
Chemical investigation of Convolvulus pluricauliswas carried out by Basu NK and .
Dandiya PC.13
6.4 OBJECTIVES OF THE STUDY
1. Collection and authentification of roots of Convolvulus pluricaulis.
2. Extraction of plant material.
3. Isolation of compounds corresponding to the major spots or peaks in TLC/HPTLC.
4. Fractionation and isolation of Marker compounds by Column Chromatography.
5. Characterization of the isolated compounds.
7. MATERIAL AND METHODS
7.1 SOURCE OF DATA:
Whole work is planned to generate data from laboratory studies i.e., experiments are performed as described in reference , and text books available within college, IISC, Bengaluru. RGUHS-Digital library and Himalaya Drug company.
Websites: .

METHOD OF DATA COLLECTION:
7.2 Plant collection and extraction:
The roots of plant will be collected and authenticated from theHimalayadrug company,Makali, Tumkur road Bengaluru.-560001

7.2DOES THE STUDY REQUIRE ANY INVESTIGATION TO BE CONDUCTED
ON PATIENTS OR ANIMALS?
-NO-

7.4 HAS ETHICAL CLEARANCE BEEN OBTAINED FROM YOUR INSTITUTION IN CASE OF 7.3?
-NOT APPLICABLE-
8. BIBILOGRAPHY:

Vinod D.Rangari. Pharmacognosy and Phytochemistry, part-II, Ist Edition. (219-220).
J.S.Quadry and Shah. Pharmacognosy 12th Edition, (401-402).
Trease and Evans. Pharmacognosy 15th Edition, (473).
Bihaqi SW,Sharma M, Singh AP, Tiwari M. Neuroprotective role of Convolvulus pluricaulis on aluminium induced neurotoxicity in rat brain. Journal Ethnopharmacol. 2009 July 30; 124(3): 409-15.
Singh RH, Narsimhamurthy K, Singh G. Neuronutrient impact of Ayurvedic Rasayana therapy in brain aging. Biogernotology. 2008 Dec; 9(6): 369-74.
DhingraD,Valecha R. Evalution of the antidepressant-like activity of Convolvulus pluricaulisin the mouse forced swim and tail suspension tests. Med Science Monit. 2007 July; 13(7).
Kumar V.Potential medicinal plants for CNS disorders. Phototherapy Res. 2006Dec; 20(12).
Sairam K, Rao CV, Goel RK.Effect of Convolvulus pluricaulison gastric ulceration and secretion in rats.Indian J Exp Biol. 2001 Apr; 39(4).
Panda S, Kar A. Inhibition of T3 production in levothyroxine-treated female mice by the root extract of Convolvulus pluricaulis. Horm Metab Res.2001 Jan; 33(1).
MudgalV. Studies on medcinal properties of Convolvulus pluricaulisand Boerhaavia diffusa.Planta Med. 1975Aug; 28(1).

Srivastava DN, Deshpande SM. Gas chromatographic identification of fatty acids, fatty alcohol and hydrocarbons of Convolvulus pluricaulis. J Am Oil Chem Soc.1975 Aug; 52(8).
Barar FS, Sharma VN. Preliminary pharmacological studies on Convolvulus pluricaulis an indigenous herb.Indian J Physiol Pharmacol.1965 Apr; 9(2).
Basu NK, Dandiya PC. Chemical investigation of Convolvulus pluricaulis.J Am Pharm Assoc Am Pharm Assoc. 1948 Jan; 37(1).

09. / NAME OF THE CANDIDATE / NARAYANA KUMAR L.G.
09.1 / SIGNATURE OF THE CANDIDATE
10. / NAME AND DESIGNATION OF THE
GUIDE / Mr.MURUGANANTHAN.G
ASST. PROFESSOR,
DEPT. OF PHARMACOGNOSY,
P.E.S.COLLEGE OF PHARMACY,BENGALURU-560 050.
10.1 / REMARKS OF THE GUIDE
10.2 / SIGNATURE OF THE GUIDE
11. / HEAD OF THE DEPARTMENT / Prof. Dr. K. LAKSHMAN
HOD, DEPT. OFPHARMACOGNOSY
P.E.S.COLLEGE OF PHARMACY
BENGALURU-560 050.
11.1 / SIGNATURE OF THE HOD
12. / PRINCIPAL / Prof. Dr. S.Mohan
PRINCIPAL & DIRECTOR,
P.E.S.COLLEGE OF PHARMACY,
BENGALURU -560050.
12.1 / SIGNATURE OF THE PRINCIPAL