Identification of RFLP and NBS/PK profiling markers for disease resistance loci in genetic maps of oats

MJ Sanz1, Y. Loarce1, A. Fominaya1, J. Vossen2, E. Ferrer1*

1 Department of Cell Biology and Genetics, University of Alcalá, Campus Universitario, 28871 Alcalá de Henares, Madrid (Spain) 2 Wageningen UR Plant Breeding, Wageningen University and Research Centre, Drovendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.

*

Supplementary Table S3. Numbers of polymorphic fragments and mapped fragments of the productive enzyme/primer combinations used for NBS/PK profiling in the Asw, MN and KO mapping populations.

Population / NBS profiling / PK profiling
Primer/enzyme combination / No polymorphic fragments / No mapped fragments / Primer/enzyme combination / No polymorphic fragments / No mapped fragments
Asw / AluI/NBS2 / 8 / 7 / AluI/PK3Fa / 11 / 11
AluI/NBS3 / 3 / 3 / AluI/PK3Fb / 15 / 14
HaeIII/NBS1 / 9 / 9 / AluI/PK4R1a / 6 / 6
HaeIII/NBS2 / 7 / 7 / AluI/PK4R1b / 10 / 10
RsaI/NBS1 / 4 / 3 / HaeIII/PK3Fa / 9 / 9
RsaI/NBS3 / 9 / 9 / HaeIII/PK3Fb / 17 / 17
- / - / - / MseI/PK4R1b / 8 / 8
- / - / - / RsaI/PK3Fa / 13 / 12
- / - / - / RsaI/PK4R1a / 11 / 11
- / - / - / RsaI/PK4R1b / 12 / 12
Total / 40 / 38 / Total / 111 / 110
MN / AluI/NBS2 / 1 / 0 / AluI/PK4R1a / 5 / 4
MseI/NBS6 / 6 / 7 / AluI/PK4R1b / 6 / 6
RsaI/NBS1 / 13 / 13 / RsaI/PK1Fa / 5 / 5
RsaI/NBS2 / 3 / 3 / RsaI/PK3Fa / 8 / 7
RsaI/NBS5A / 5 / 6 / RsaI/PK4R1a / 4 / 4
- / - / - / RsaI/PK4R1b / 9 / 9
Total / 28 / 29 / Total / 38 / 35
KO / AluI/NBS1 / 10 / 7 / AluI/PK1Fa / 4 / 2
AluI/NBS2 / 5 / 4 / AluI/PK1Fb / 5 / 3
AluI/NBS5A / 4 / 3 / AluI/PK3Fa / 13 / 10
AluI/NBS6 / 6 / 1 / AluI/PK3Fb / 5 / 4
HaeIII/NBS1 / 10 / 6 / AluI/PK4R1a / 18 / 15
HaeIII/NBS2 / 11 / 6 / AluI/PK4R1b / 16 / 10
HaeIII/NBS3 / 5 / 4 / HaeIII/PK1Fa / 5 / 4
HaeIII/NBS5A / 7 / 5 / HaeIII/PK1Fb / 4 / 2
HaeIII/NBS6 / 5 / 6 / HaeIII/PK3Fa / 5 / 2
RsaI/NBS1 / 5 / 3 / HaeIII/PK3Fb / 7 / 3
RsaI/NBS2 / 11 / 7 / HaeIII/PK4R1a / 9 / 8
RsaI/NBS3 / 6 / 4 / HaeIII/PK4R1b / 9 / 6
RsaI/NBS5A / 9 / 6 / RsaI/PK1Fa / 11 / 10
RsaI/NBS6 / 4 / 2 / RsaI/PK1Fb / 5 / 3
- / - / - / RsaI/PK3Fa / 9 / 7
- / - / - / RsaI/PK3Fb / 10 / 6
- / - / - / RsaI/PK4R1a / 21 / 19
- / - / - / RsaI/PK4R1b / 13 / 9
Total / 98 / 64 / Total / 169 / 123