Felkin Et Al., Online Resource 1

Felkin et al., Online Resource 1

Assay / αMHC
Gene name / α Myosin Heavy Chain
Accession Number / D00943, BC117511, BC132667
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00411908_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ACTA1
Gene Name / Actin, alpha 1, skeletal muscle
Accession Number / NM_001100
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00559403_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ACTA2
Gene Name / Actin, alpha 2, smooth muscle
Accession Number / NM_001141945
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00909449_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ADRB1
Gene Name / Adrenergic receptor, beta 1
Accession Number / NM_000684
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00265096_s1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ADRB2
Gene Name / Adrenergic receptor, beta 2
Accession Number / NM_000024
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00240532_s1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ANP
Gene name / Atrial natriuretic peptide
Accession Number / M30262
Forward Primer / 5’ tca gac cag agc taa tcc cat gta c 3’
Taqman Probe / 5’ cca gca aat tct tga aat cca tca ggt ctg 3’
Reverse Primer / 5’ cga cct cat ctt cta aag gca tct 3’
Forward/Probe/Reverse, nM / 300/175/50
Assay / βMHC
Gene name / β Myosin Heavy Chain
Accession Number / NM_000257
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00165276_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / BNP
Gene name / Brain natriuretic peptide
Accession Number / M31776
Forward Primer / 5’ gag gaa gat gga ccg gat ca 3’
Taqman Probe / 5’ cag cac ttt gca gcc cag gcc a 3’
Reverse Primer / 5’ tgt gga atc aga agc agg tgt ct 3’
Forward/Probe/Reverse, nM / 300/150/300
Assay / CD68
Gene name
Accession Number / NM_001251, NM_001040059
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00154355_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / COL1A1
Gene name / Collagen1 α 1
Accession Number / NM_000088
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00164004_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / COL3A1
Gene name / Collagen 3 α 1
Accession Number / NM_000090
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00164103_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / CTGF
Gene name / Connective Tissue Growth Factor
Accession Number / NM_001901
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00170014_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / EPAC2
Gene name / Exchange factor directly activated by cAMP 2
Accession Number / NM_007023
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00199754_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / FN
Gene name / Fibronectin
Accession Number / NM_212474, NM_212475, NM_212476, NM_212478, NM_212482, NM_002026
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs01549940_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / FST
Gene name / Follistatin
Accession Number / NM_006350
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00246260_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / FSTL1
Gene name / Follistatin-like 1
Accession Number / NM_007085
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00200053_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / FSTL3
Gene name / Follistatin-like 3
Accession Number / NM_005860
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00610505_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / GATA4
Gene name / GATA binding protein 4
Accession Number / NM_002052
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00171403_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / GATM
Gene name / Glycine amidinotransferase
Accession Number / NM_001482
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00155208_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / Giα2
Gene name / Guanine nucleotide binding protein , alpha inhibitor 2
Accession Number / X04828
Forward Primer / 5' gct caa ggg aat acc agc tca a 3'
Taqman Probe / 5’ act cag ctg cct act acc tga acg acc tgg 3’
Reverse Primer / 5’ gcg ggt ccg tag cac atc t 3’
Forward/Probe/Reverse, nM / 300/100/300
Assay / HAND1
Gene name / Heart and neural crest derivatives expressed transcript 1
Accession Number / NM_004821
Forward Primer / 5' CGT GAG AGC AAG CGG AAA A 3'
Taqman Probe / 5' AAA ACC TTC GTG CTG CTG CAG CTC C 3'
Reverse Primer / 5' CCG GTG CGT CCT TTA ATC CT 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / HAND2
Gene name / Heart and neural crest derivatives expressed transcript 2
Accession Number / NM_021973
Forward Primer / 5' GAT CAA GAA GAC CGA CGT GAA AG 3'
Taqman Probe / 5' CAA GAT TTC GTT CAG CTC CTT CTT CCT CTT CTC 3'
Reverse Primer / 5' CGT TGC TGC TCA CTG TGC TT 3'
Forward/Probe/Reverse, nM / 900/100/900
Assay / IGF1
Gene name / Insulin-like Growth Factor 1
Accession Number / NM_000618
Forward Primer / 5' TCC GAC TGC TGG AGC CAT A 3'
Taqman Probe / 5' TGG GCT TGT TGA AAT AA 3' (MGB)
Reverse Primer / 5' GCT GGT GGA TGC TCT TCA GTT 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / IGF2
Gene name / Insulin-like Growth Factor 2
Accession Number / NM_000612
Applied Biosystems’ Taqman gene expression assay
Hs00171254_m1
Forward Primer
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / IL-1β
Gene name / Interleukin 1β
Accession Number / NM_000576
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00174097_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / IL-6
Gene name / Interleukin 6
Accession Number / M54894
Forward Primer / 5’ tga caa aca aat tcg gta cat cct 3’
Taqman Probe / 5’ tta ctc ttg tta cat gtc tcc ttt ctc agg gct g 3’
Reverse Primer / 5’ agt gcc tct ttg ctg ctt tca c 3’
Forward/Probe/Reverse, nM / 300/175/300
Assay / IL-10
Gene name / Interleukin 10
Accession Number / Not known
Forward Primer / Applied Biosystems’ Taqman gene expression assay (discontinued)
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / ITGAX
Gene name / Integrin, alpha X
Accession Number / NM_000887
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00174217_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / KLF15
Gene name / Kruppel-like factor 15
Accession Number / NM_014079
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00362736_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / MCP-1
Gene name / Monocyte Chemotatic Protein 1
Accession Number / NM_002982
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00234140_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / MMP1
Gene name / Matrix Metalloproteinase 1
Accession Number / NM_002421
Forward Primer1 / 5' AGC TAG CTC AGG ATG ACA TTG ATG 3'
Taqman Probe1 / 5' CAT CCA AGC CAT ATA TGG ACG TTC CCA AA 3'
Reverse Primer1 / 5' CTC CCC GAA TCG TAG TTA TAG CAT 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / MMP2
Gene name / Matrix Metalloproteinase 2
Accession Number / NM_004530
Forward Primer / 5' CGC AGA TGC CTG GAA TGC 3'
Taqman Probe / 5' TGA CCG CCG CCC TGC AGG 3'
Reverse Primer / 5' TCA GGT AAT AGG CAC CCT TGA AG 3'
Forward/Probe/Reverse, nM / 500/200/500
Assay / MMP8
Gene name / Matrix Metalloproteinase 8
Accession Number / NM_002424
Forward Primer / 5'CCA AAG AGA TCA CGG TGA CAA 3'
Taqman Probe / 5'CCA TTT GAT GGA CCC AAT GGA ATC CTT G 3'
Reverse Primer / 5' CCT GGC TGA AAG GCA TGA G 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / MMP9
Gene name / Matrix Metalloproteinase 9
Accession Number / NM_004994
Forward Primer3 / 5' CCC TGG AGA CCT GAG AAC CA 3'
Taqman Probe3 / 5' ATT CCT CTG CCA GCT GCC TGT CGG 3'
Reverse Primer3 / 5' CCA CCC GAG TGT AAC CAT AGC 3'
Forward/Probe/Reverse, nM / 300/200/300
Assay / MMP14
Gene name / Matrix Metalloproteinase 14
Accession Number / NM_004995
Forward Primer / 5' AGA TCA AGG CCA ATG TTC GAA 3'
Taqman Probe / 5' CAT CCA GGG TCT CAA ATG GCA ACA TAA TGA A 3'
Reverse Primer / 5' GGT GTA ATT CTG GAT GCA GAA AGT G 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / nNOS
Gene name / Nitric oxide synthase 1 (neuronal)
Accession Number / NM_000620
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00167223_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / PECAM-1
Gene name / Platelet/endothelial cell adhesion molecule 1
Accession Number / NM_000442
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00169777_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / RANTES
Gene name / Regulated upon activation, normal T-cell expressed, and secreted
Accession Number / NM_002985
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00174575_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / RGS3
Gene name / Regulator of G-protein Signalling 3
Accession Number / U27655
Forward Primer / 5’ aag gca gac aaa atg atg aag tca 3’
Taqman Probe / 5’ tgc taa ccc gta ttt gtg aac cag cag ct 3’
Reverse Primer / 5’ gtg cga agg aag gct tgg a 3’
Forward/Probe/Reverse, nM / 300/125/50
Assay / RGS4
Gene name / Regulator of G-protein Signalling 4
Accession Number / U27768
Forward Primer / 5’ gac aaa gtg gtt att tgc cag aga gt 3’
Taqman Probe / 5’ aag ctg cca gcc cac att cat gac taa tc 3’
Reverse Primer / 5’ tcc tca cta tat tca gac ttc aag aaa gc 3’
Forward/Probe/Reverse, nM / 300/150/300
Assay / SDF1
Gene name / Stromal cell-derived factor 1
Accession Number / NM_000609
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00171022_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / SFRP1
Gene name / Secreted frizzled receptor protein 1
Accession Number / NM_003012
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00610060_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / TBX2
Gene name / T-box protein 2
Accession Number / NM_003012
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00172983_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / TGFβ1
Gene name / Transforming growth factor β1
Accession Number / NM_000660
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00171257_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / THY1
Gene name / Thymus cell antigen 1
Accession Number / NM_006288
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00174816_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / TIMP1
Gene name / Tissue inhibitor of MMP 1
Accession Number / NM_003254
Forward Primer / 5' CAC CCA CAG ACG GCC TTC T 3'
Taqman Probe / 5' CAA TTC CGA CCT CGT CAT CAG GGC 3'
Reverse Primer / 5' TCT GGT GTC CCC ACG AAC TT 3'
Forward/Probe/Reverse, nM / 900/150/900
Assay / TIMP2
Gene name / Tissue inhibitor of MMP 2
Accession Number / NM_003255
Forward Primer / 5' CCT GAA CCA CAG GTA CCA GAT G 3'
Taqman Probe / 5' TGC AAG ATC ACG CGC TGC CC 3'
Reverse Primer / 5' AGG AGA TGT AGC ACG GGA TCA 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / TIMP3
Gene name / Tissue inhibitor of MMP 3
Accession Number / NM_00362
Forward Primer / 5' GCC TTA AGC TGG AGG TCA ACA 3'
Taqman Probe / 5' CCA TCA TAG ACG CGA CCT GTC AGC AG 3'
Reverse Primer / 5' GCA CAG CCC CGT GTA CAT CT 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / TIMP4
Gene name / Tissue inhibitor of MMP 4
Accession Number / NM_003256
Forward Primer / 5' CAC CCT CAG CAG CAC ATC TG 3'
Taqman Probe / 5' ACT CGG CAC TTG TGA TTC GGG CC 3'
Reverse Primer / 5' GGC CGG AAC TAC CTT CTC ACT 3'
Forward/Probe/Reverse, nM / 900/200/900
Assay / TLR4
Gene name / Toll Like Receptor 4
Accession Number / XM_005336
Forward Primer / 5’ tat agc ttc ttc agt ttc cca gaa ctg 3’
Taqman Probe / 5’ tgt ctg gat ttc aca cct gga taa atc cag c 3’
Reverse Primer / 5’ ggc tct gat atg ccc cat ctt 3’
Forward/Probe/Reverse, nM / 300/175/300
Assay / TNFα
Gene name / Tumour Necrosis Factor α
Accession Number / Not known
Forward Primer / Applied Biosystems’ Taqman gene expression assay
4311181 (discontinued)
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated
Assay / VWF
Gene name / Von Willebrand Factor
Accession Number / NM_000552
Forward Primer / Applied Biosystems’ Taqman gene expression assay
Hs00169795_m1
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Not known
Assay / 18S rRNA
Gene name / 18S ribosomal RNA
Accession Number / Not known
Forward Primer / Applied Biosystems’ Taqman gene expression assay
4310893E
Taqman Probe
Reverse Primer
Forward/Probe/Reverse, nM / Assay supplied optimised and validated for multiplex analysis

Online Resource 1. Taqman assay details.