Appendix 1.New chromosome counts and estimations of DNA-ploidy level in Hieracium alpinum s.str.

Country abbreviations (AUT – Austria, BIH – Bosnia and Herzegovina, FIN – Finland, CHE – Switzerland, ITA – Italy, NOR – Norway, ROM – Romania, SVK – Slovakia, UKR – Ukraine).

New chromosome counts (counted by P. Mráz):

2n=2x=18

1. UKR, Eastern Carpathians, Skhidni Beskidi Mts, Mt. Pikuï, on the ridge ca 0.3 km W of the summit, 48°49'52"N, 22°59'45"E, 1395 m a.s.l., coll. P. Mráz and J. Chrtek, 21 July 2003 (1 plant, cult. no. 1422).

2n=3x=27

1. AUT, Steiermark, Hochschwab, Präbichl, above Leobner Hütte, 1680 m a.s.l., 47°32'01"N, 14°58'20"E, coll. P. Mráz and J. Chrtek, 25 July 2005 (1 plant, cult. no. 1767).

2. CHE, Valais, Col du Grand St. Bernard, 2562 m a.s.l., 45°52'02"N, 07°10'19"E, coll. P. Mráz, 25 August 2005 (2 plants, cult. nos. 1822, 1823).

New estimations of DNA ploidy level by means of flow cytometric analysis (in bold letters are the cultivation numbers of plants, or only the number of plants, used for measurements of total DNA content, analyses done by P. Mráz and B. Šingliarová):

2n ~ 2x

1. ROM, Southern Carpathians, Munţii Capaţinii, Mt. La Nedea, slopes below the summit, 2140 m a.s.l., 45°19'40" N, 23°50'46" E, 4 August 2006, coll. B. Šingliarová (3 plants).

2. ROM, Southern Carpathians, Munţii Bucegi, Mt. Caraiman, meadows around the monument (iron cross), 2273 m a.s.l., 45°25'25" N, 25°29'09" E, 16 July 2007, coll. B. Šingliarová and R. Lakoštík (4 plants).

2n ~ 3x

1. AUT, Kärnten, Gurktaler Alpen, Turracher Höhe, Panorama Alm, 1979 m a.s.l., 46°55'03" N, 13°51'57" E, coll. P. Mráz and J. Chrtek, 27 July 2005 (3 plants, cult. nos. 1783, 1784, 1785).

2. AUT, Kärnten, Hohe Tauern, Kolbnitz, Reißeckhütte, S of the tunnel, 2225 m a.s.l., 46°55'00" N, 13°21'41" E, coll. P. Mráz, 29 July 2005 (3 plants, cult. nos. 1798, 1799, 1800).

3. AUT, Steiermark, Hochschwab, Präbichl, above Leobner Hütte, 1680 m a.s.l., 47°32'01" N, 14°58'20" E, coll. P. Mráz and J. Chrtek, 25 July 2005 (3 plants, cult. nos. 1765, 1766, 1767).

4. AUT, Steiermark, Stubalpe, Köflach, ca 0.4 km SW from Wölkerkogel, not far from Altes Almhaus, 1687 m a.s.l., 47°04'52" N, 14°55'17" E, coll. P. Mráz and J. Chrtek, 25 July 2005 (2 plants, cult. nos. 1803, 1804).

5. AUT, Austria, Tirol, Defereggengebirge, Stallersattel, E of the glacial lake, 2043 m a.s.l., 46°53'23" N, 12°12'33" E, coll. P. Mráz and J. Chrtek, 29 July 2005 (1 plant, cult. no. 1762).

6. BIH, Vranica planina Mts, in the saddle between Mt. Nadkrstac and Mt. Vranica, small hill in the dwar-pine communities, 2099 m a.s.l., 43°57'17" N, 17°43'32" E,coll. B.Šingliarová and R. Lakoštík, 12 July 2006 (1 plant, 3 plants).

7. FIN, Lapin Läämi, Mt. Malla, N of the lake Kilpisjärvi, 670 m a.s.l., 69°03'22" N, 20°45'11" E, coll. P. Mráz and R. Mráz, 13 August 2006 (2 plants).

8. CHE, Albula Alpen, Davos, Flüelapass, 2354 m a.s.l., 46°44'36" N, 09°57'54" E, coll. P. Mráz and J. Košúth, 22 August 2005 (2 plants, cult. nos., 1826, 1828).

9. CHE, Albula Alpen, St. Moritz, Julierpass, 2355 m a.s.l., 46°27'54" N, 09°42'50" E, coll. P. Mráz and J. Košúth, 22 August 2005 (2 plants, cult. nos. 1841, 1842).

10. CHE, Graubünden, NE slopes of Mt. Rossbodenstock, above Oberalpass, 2253 m a.s.l., 46°39'10" N, 08°40'02" E, coll. P. Mráz and J. Košúth, 23 August 2005 (1 plant, cult. no. 1830).

11. CHE, Spluga, Passo dello Spluga (Splügenpass), 2120 m a.s.l., 46°30'21" N, 09°19'54" E, coll. P. Mráz and J. Košúth, 23 August 2005 (2 plants, cult. nos. 1843, 1844).

12. CHE, Wallis, Oberwald, Furkapass, ca 200 m S of the saddle, exposed slopes with Salix herbacea, 2457 m a.s.l., 46°34'22" N, 08°24'49" E, coll. P. Mráz and J. Košúth, 24 August 2005 (2 plants, cult. nos. 1836, 1837).

13. CHE, Walliser Alpen, Col du Grand St. Bernard, above the saddle, 2562 m a.s.l., 45°52'02" N, 07°10'19" E, coll. P. Mráz, 25 August 2005 (5 plants, cult. nos. 1821, 1822, 1823, 1824, 1825).

14. ITA, Alpi Sarentine, Vipiteno, Passo di Giovo (Jaufenpass), 2144 m a.s.l., 46°50'29" N, 11°19'06" E, coll. P. Mráz and J. Chrtek, 31 July 2005 (3 plants, cult. nos. 1789, 1790, 1791).

15. ITA, Alpi Sarentine, Vipiteno, Passo di Pénnes (Penser Joch), 2210 m a.s.l., 46°49'04" N, 11°26'00"E, coll. P. Mráz and J. Chrtek, 31 July 2005 (2 plants, cult. nos. 1792, 1793).

16. ITA, Ortler Gruppe, Bormio, Passo dello Stelvio (Stilfser Joch), W of the saddle, 2600 m a.s.l., 46°33'17" N, 10°25'25" E, coll. P. Mráz, 21 August 2005 (4 plants, cult. nos. 1832, 1833, 1834, 1835).

17. NOR, Telemark, Rjukan, the saddle below Mt. Gaustakane, S slopes ca 1 km E of the saddle, 1270 m a.s.l., 59°49'06" N, 08°43'20" E, coll. P. Mráz and R. Mráz, 7 August 2006 (1 plant).

18. NOR, Sør-Trøndelag, S of the town of Oppdal, valley of Vinstra, NE border of the Dovrefjell nasjonal park, 1269 m a.s.l., 62°27'21" N, 09°41'08" E, coll. P. Mráz and R. Mráz, 8 August 2006 (2 plants).

19. SVK, Western Carpathians, Západné Tatry Mts, Trnovecká dolina valley, tourist path to Mt. Baranec, 1885 m a.s.l., 49°09'47" N, 19°44'04" E, coll. B. Šingliarová and R. Lakoštík, 27 July 2007 (4 plants).

20. SVK, Western Carpathians, Vysoké Tatry Mts, Furkotská dolina valley, 1900 m a.s.l., 49°09'10" N, 20°01'40" E, coll. B. Šingliarová and R. Lakoštík, 18 August 2005 (2 plants).