Cell Biology Laboratory
PCB3023L (7781)
Fall 2017 R 4:00 to 6:40 PM CL/SS-136
Instructor: Katherine L Siegler
Email:
Office Hours: Seminole LSC M/W 10:45 to 12:00; T/R Clearwater NM131 12:30 to 1:45 PM, or by appointment
Office: NM131 Clearwater campus
Academic Department:
Dean: Natavia Middleton
Office Location: UP 337B (Seminole Campus)
Office Number: 727-398-8288
Academic Chair: Dr. Linda Gingerich
Office Location: NM 213
Office Number: 727-791-2538
Pre-requisites/ Course Description:
This laboratory course will consist of experiments that teach and apply fundamental techniques used in cell biology such as isolation and study of cellular organelles, protein isolation, separation, activity and detection.
Pre-requisites: BSC2011 with a minimum grade of C, BSC2011L with a minimum grade of C, CHM2211 with a minimum grade of C, CHM2211L with a minimum grade of C.
Course objectives: Curriculum and Instruction document is located in MyCourses
Criteria Performance Standard:
Upon the completion of this course the student will, with a minimum 70 percent accuracy, demonstrate mastery of each of the stated objectives through classroom measures developed by individual course instructors.
Student/Classroom Expectations:
Students are responsible for reading assigned laboratory protocol and completing any pre-laboratory quizzes.
Students are advised to establish regular weekly study habits and seek tutoring help, if necessary, early in the semester. The concepts from one week build a framework for the next week. Contact me at any time with concerns by phone or email.
This syllabus is currently available in MY COURSES for your convenience
Textbook:
Required Text: None, online content available on MyCourses
Meeting Information: CL/SS-136
Meeting Days: R
Class Times: 4:00 PM to 6:40 PM
COLLEGE CALENDAR: http://www.spcollege.edu/calendar/
Important Dates:
August 14 Classes begin
August 18 Drop Add date
August 18 Last day to withdraw with refund
September 4 Labor Day – NO CLASSES
October 31 All College Day – NO CLASSES
October 19 Last day to withdraw with a grade of “W”
November 10 Veterans’ Day – NO CLASSES
November 22 – 26 Thanksgiving Break
December 4 – 7 Final Exams
Note to students: The syllabus addendum is an important part of your syllabus and can be easily accessed by using the link below. Do take the time to read this very important information— http://www.spcollege.edu/addendum/
Academic Integrity: The college has an official policy on academic honesty and proper classroom behavior. It is the student’s responsibility to review the online Academic Honesty Policy or "Academic Honesty and Student Behavior: Expectations of Students at SPC" brochure. It is important to remember that everyone’s goal should be to learn. Behavior that impedes the learning process of others will not be tolerated. Disruptive behavior includes talking at inappropriate times, text-messaging or talking on the phone during class or repeatedly coming to class late. Students are expected to be actively engaged in the learning process, and should ask the instructor questions as needed. The professor will assign a grade of "0" to any exam or other course work for admitted or alleged academic dishonesty pending appeal. Penalties may include expulsion from college.
Attendance Policy: The college-wide attendance policy is included in the Syllabus Addendum http://www.spcollege.edu/webcentral/policies.htm.
For this class, attendance is defined as performing the laboratory exercise, data collection and analysis. Attendance at all laboratory sessions is essential (Since you are working with biological materials and labs that require multiple lab sessions, therefore make-ups may be impossible). If you miss a lab you can’t submit a lab report and will receive a zero for that assignment.
Any student who misses three consecutive labs will receive a grade of WF for excessive absences.
REQUESTS TO MAKE UP LABS AND LAB EXAMS MUST BE COMMUNICATED IN ADVANCED AND MAY REQUIRE DOCUMENTATION.
Faculty must file lack of attendance reports (exceeding 2 absences) for veterans, dual-enrolled, Early College and Collegiate High School students.
SPC Withdrawal Policy:
Instructors can no longer withdraw students from classes. If a student wishes to withdraw from a course, it is the responsibility of the student to withdraw either online through MySPC or with the help of an advisor in the registration office. If the withdrawal is completed by October 19, 2017, a grade of “W” will be recorded which does not impact the student’s GPA. If the withdrawal occurs after October 19, 2017 the student will receive a grade of WF (withdraw-failing). The WF grade is punitive; it has the same impact on the GPA as an F. Students in the third attempt of a course who withdraw will receive a WF regardless of the date of withdrawal. Students who do not attend during the first two weeks of classes will automatically be withdrawn from the course by the college and will still be responsible for the tuitionand fees. Also, students who do not show sufficient active participation in the course by October 19, 2017, will be administratively withdrawn by the college and receive a WF. Students who withdraw from all classes may be required to repay some or all of the financial aid received for the term. Should you consider totally withdrawing from all classes before October 19, 2017 (for the Fall session) it is important that you consult the Scholarships & Student Financial Assistance office on your home campus to understand your options and the consequences of total withdrawal.
Disability Statement: St. Petersburg College provides reasonable accommodations for students with disabilities in compliance with Title II of the Americans with Disabilities Act of 1990. A student who is in need of one or more accommodations for a disability must make a request either verbally or in writing to the College. In the event that a request is made directly to an instructor or other staff member, the instructor or staff member must refer the student to the Disabilities Office. The student must self-identify the disability by completing the Self Identification and Authorization Form for Students with Disabilities (SDS-1) as well as provide documentation of the disability that complies with the SPC published Guidelines for Documentation of a Disability. Confidentiality is strictly maintained for any records provided to the Office of Disabilities Services related to the student’s disability. Under the law, SPC is allowed to request documentation that reflects the current functioning of the student.
Grading System
This course is a skills based course relying heavily on completing assignments and participation in laboratory experiments. The final grade is calculated as follows: 1) There will be two lab exams each worth 100 points, 2) Pre-lab quizzes (10 total, each worth 10 points), 3) Lab reports (4 total, each worth 25 points).
All assignments must be completed and submitted to the appropriate dropbox at the scheduled time. Discussion participation and any associated activities must be completed on time and CAN NOT be made up due to excused or unexcused absence. Details and any changes to specific assignments will be posted in the Lessons folder on MY COURSES.
Wikipedia may not be used as a reference in any assignment.
Late assignments will not be accepted for any reason.
Further details will be provided during class or posted on MyCourses.
Exams:
The lab exams will focus on the material presented in the lab with some reading material from your lab handouts. The exams will consist of a combination of multiple choice, fill in the blank, short answer and data analysis questions. Lab exams may also include a “practical” portion, which will have various stations containing experimental data for you to interpret.
Any student missing an exam may take a make-up provided they have a legitimate excuse for missing class (severe illness with a doctor’s note, family emergency, etc). All scores on make-up exams will be reduced by 10% each time you need to take one. This penalty applies to ANYONE taking a make-up, No exceptions! The 10% penalty is additive, so the first make-up is 10%, the 2nd - 20%, the 3rd – 30%, etc. The make-up exam must be taken within one week of the scheduled lab exam.
Pre-lab Quizzes:
Pre-lab quizzes will be found in MyCourses. Quizzes must be completed one hour prior to the lab session (3:00 PM). The quizzes are designed to make sure that you understand the basic concepts and techniques that will be performed in lab.
Lab Reports:
Lab reports are assigned through out the semester to help you understand the experiments. Refer to the schedule and the MyCourses calendar for the due dates. Reports are due in the appropriate dropbox one hour before (3:00 PM) the start of the lab on the due date. Wilipedia may not be used as a reference in any assignment.
Late assignments will not be accepted for any reason.
The following is the point distribution for this course:
Lab exams / 200 pointsPre-lab quizzes / 100 points
Lab reports / 100 points
Grading Scale:
360 – 400 points / A320 – 359 points / B
280 – 319 points / C
260 – 279 points / D
< 260 points / F
Student Survey of Instruction (SSI):
The student survey of instruction is administered in courses each semester. It is designed to improve the quality of instruction at St. Petersburg College. All student responses are confidential and anonymous and will be used solely for the purpose of performance improvement.
Course Schedule
Lab / Topic1 / 8/17 / Introduction to Cell Biology lab; Requirements; Lab safety
2 / 8/24 / Pipetting practice
3 / 8/31 / Standard Curve - Protein determination
4 / 9/7 / Factors effecting enzyme activity – Catalase
Lab Report #1: Standard Curve (Lab 3)
5 / 9/14 / Enzyme activity and inhibitors
6 / 9/21 / Calculations
7 / 9/28 / Chromatography - Gel-Filtration Chromatography– Part one: Isolation
Lab Report #2: Enzyme Activity (Lab 4 and 5)
8 / 10/5 / Chromatography - Gel-Filtration Chromatography– Part two: Identification by enzyme activity
9 / 10/12 / Midterm Lab Exam
10 / 10/19 / Photosynthesis and Respiration
Lab Report #3: Protein Isolation and Identification (Lab 7 and 8)
11 / 10/26 / Identification of macrophage migration inhibitory factor in cell lysates – Part one: Cell counting and lysate preparation
12 / 11/2 / Identification of macrophage migration inhibitory factor in cell lysates – Part two: Protein concentration
13 / 11/9 / Identification of macrophage migration inhibitory factor in cell lysates – Part three: Western blot set up
14 / 11/16 / Identification of macrophage migration inhibitory factor in cell lysates – Part four: Identification by Western blot analysis
11/23 / Thanksgiving – No class
15 / 11/30 / Final Lab Exam
Lab Report #4: Identification of macrophage migration inhibitory factor (Lab 11, 12, 13, 14)
Note: Instructor reserves right to change above information, including, but not limited to, test dates and information covered. It is the student’s responsibility to be aware of changes. Instructor will give sufficient notice regarding changes.
After reading this syllabus, read the below signature page, sign it (type your name) and upload it into the dropbox in MyCourses by the end of the second week of class (August 25, 2017)
Signature Page
I have read, understand, and agree to abide fully by the parameters set in this syllabus and Syllabus Addendum.
Student Signature: Date: .