Datasheet
Jurkat-TIM3
Catalog#: YMAK-C012
Cell line ID: Jurkat-TIM3
Species: Human
Cell Type: Lymphocyte
Description: Jurkat cell line stably expressing exogenous human TIM3 gene.
Gene ID/Accession#: BC063431
Selection Marker: G418 selection system
Application: TIM3 mAb screening and biological assays
Morphology: Lymphoblast, Suspension
Medium: 1640+10%FBS
Subculture: Cultures can be maintained by the addition of fresh medium or replacement of medium.Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1×105 viable cells/mL. Do not allow the cell density to exceed 3×106 cells/mL.
Incubation: 37℃With 5% CO2
Storage: Frozen in liquid nitrogen with 70% 1640 medium, 20% FBS and 10% DMSO
Doubling Time: ~24 hours
Biosafety level: 1
References: N/A
Background
Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function.
Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.
Cell Line Generation
Jurkat-TIM3 Cell was generated using pMac-TIM3 vector expressing human TIM3 gene based on in house Jurkat cell line.
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Datasheet
Characterization
FACS analysis of cell surface TIM3 expression by an anti-human TIM3 antibody.
Application
TIM3 mAb screening with FACS.
Binding of TIM3 mAb to Jurkat-TIM3 by FACS analysis.
Cell Line Limited Use License
FOR IN VITRO REASEARCH USE ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE. BY ACCEPTING THIS
PRODUCT, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT.
This product may not be resold or transferred by the recipient and may be used only by the recipient, in their facility and only for (1) research use, (2) drug discovery and development of biologic drugs, (3) quality assurance testing of biologic drugs, and (4) assays for biologic drugs. No other commercial use is allowed. “Commercial” use means any and all uses of this product by recipient for monetary or other consideration, including providing a service, supplying information or data to unaffiliated third parties, and resale of this product for any use. Recipient has no right to modify, derivatize, genetically engineer or otherwise create variations of the Cells.
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