Online resource 1. Analysis of variance for measured and calculated traits using model 1. Parents are excluded from the analysis.

Effects
Treatment / Block(treatment) / Genotype / Treatment x genotype / Error
F-ratio. sign. Level b). pair-wise test c) / F-ratio. sign. level b) / F-ratio. sign. level b) / F-ratio. sign. level b) / MS
df / 2 / 6 / 197 (196) d) / 394 (392) d) / 1119-1182
Measured traits a) / Type of trait a)
AL / B / 597.6 *** a / 48.8 *** / 7.79 *** / 1.31 *** / 36.33
BL / B / 341.6 *** a / 56.2 *** / 3.30 *** / 1.22 ** / 0.233
BS / B / 402.2 *** b / 91.5 *** / 3.52 *** / 1.32 *** / 0.264
SPAD1 / N / 39.91 *** c / 116.2 *** / 4.06 *** / 1.17 * / 22.57
SPAD2 / N / 285.0 *** b / 42.4 *** / 4.72 *** / 1.14 * / 11.68
SPAD3 / N / 55.6 *** b / 32.0 *** / 4.15 *** / 0.97 ns / 13.66
Calculated traits a) / Type of trait a)
BL/BS / B / 113.0 *** b / 40.3 *** / 4.04 *** / 0.93 ns / 0.042
EA / B / 33.6 *** a / 34.3 *** / 3.50 *** / 1.32 *** / 243.0
EN / N / 23.9 *** c / 122.2 *** / 3.72 *** / 1.06 ns / 0.066
NA / N / 141.8 *** b / 55.8 *** / 3.51 *** / 1.17 * / 37.41
NL / N / 197.2 *** a / 30.2 *** / 3.00 *** / 1.08 ns / 0.539
NM / N / 123.5 *** a / 83.6 *** / 5.38 *** / 1.13 ns / 0.018
RSPAD / N / 31.4 *** c / 66.4 *** / 2.20 *** / 1.12 ns / 0.066

a) For trait abbreviations see Table 1

b) significance levels *** = p < 0.001. ** = p < 0.01. * = p < 0.05. ns = non significant

c) significant difference between treatments based on pair-wise t-test; a = sign. difference between all treatments. b = sign. difference between well

watered treatments and water stressed treatment. c = sign. difference between high nutrient well water treatment and low nutrient well

watered treatment.

d) df for the traits NA, NL and NM in parenthesis