Biometals, full paper
Title: Isolation and structure determination of new siderophore albachelin from Amycolatopsis alba
Authors: Shinya Kodani*,1,Hisayuki Komaki2,Masahiro Suzuki1,Hikaru Hemmi3,Mayumi Ohnishi-Kameyama3
Affiliations and addresses:
1Graduate School of Agriculture, Shizuoka University, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
2Biological Resource Center, National Institute of Technology and Evaluation (NBRC), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292‒0818, Japan
3National Food Research Institute, National Agriculture and Food Research Organization (NARO), 2–1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan
*To whom correspondence should be addressed: Shinya Kodani, Tel/Fax; +81(54)238-5008, E-mail;
Supporting information
Figure S1. ESI-TOF mass spectrum of ferri-albachelin
Figure S2. ESI-TOF mass spectrum of gallium-complex of albachelin
Figure S3. ESI-FT-ICR mass spectrum of desferri-albachelin
Figure S4. MALDI-TOF MS/MS spectrum of desferri-albachelin
Figure S5. 1H NMR spectrum of Ga-albachelin in DMSO-d6
Figure S6. 13C NMR spectrum of Ga-albachelin in DMSO-d6
Figure S7. DEPT-135 spectrum of Ga-albachelin in DMSO-d6
Figure S8. DQF-COSY spectrum of Ga-albachelin in DMSO-d6
Figure S9. TOCSY spectrum of Ga-albachelin in DMSO-d6
Figure S10. NOESY spectrum of Ga-albachelin in DMSO-d6
Figure S11. HSQC spectrum of Ga-albachelin in DMSO-d6
Figure S12. HMBC spectrum of Ga-albachelin in DMSO-d6
Figure S13. 1H-15N HSQC spectrum of Ga-albachelin in DMSO-d6
Table S1.Putative functions of proteins encoded by genes within the putative albachelin biosynthetic gene cluster
Figure S1. ESI-TOF mass spectrum of ferri-albachelin
Figure S2. ESI-TOF mass spectrum of gallium-complex of albachelin
Figure S3. ESI-FT-ICR mass spectrum of desferri-albachelin
Figure S4. MALDI-TOF MS/MS spectrum of desferri-albachelin
Figure S5. 1H NMR spectrum of Ga-albachelin in DMSO-d6
Figure S6.13C NMR spectrum of Ga-albachelin in DMSO-d6
Figure S7. 13C DEPT135 spectrum of Ga-albachelin in DMSO-d6
Figure S8. DQF-COSY spectrum of Ga-albachelin in DMSO-d6
Figure S9. TOCSY spectrum of Ga-albachelin in DMSO-d6
Figure S10. NOESY spectrum of Ga-albachelin in DMSO-d6
Figure S11. HSQC spectrum of Ga-albachelin in DMSO-d6
Figure S12. HMBC spectrum of Ga-albachelin in DMSO-d6
Figure S13. 1H-15N HSQC spectrum of Ga-albachelin in DMSO-d6
TableS1. Putative functions of proteins encoded by genes within the putative albachelin biosynthetic gene cluster
Closest homolog (accession no.); origin / %* / S. erythraea (erythrochelin) / S. coelicolor (coelichelin) / S. scabies (scabichelin)
AMYAL_RS 0130200 / WP_020635017 / 315 / formyltransferase / formyltransferase (EMD22242); Amycolatopsis azurea DSM 43854 / 96/98 / SACE_2691; 67/83 / SCO0499 (CchA); 71/83 / SCAB_85511; 76/87
AMYAL_RS 0130205 / WP_020635018 / 443 / lysine/ornithine N-monooxygenase / L-lysine 6-monooxygenase (KFU82303); Amycolatopsis lurida NRRL 2430 / 96/98 / SACE_3033 (ErcB); 69/78 / SCO0498 (CchB); 67/78 / SCAB_85521; 58/70
AMYAL_RS 0130210 / WP_020635019 / 7,406 / NRPS (C/A/T-C/A/T/E-C/A/MT/T-C/A/T-C/A/T-C/A/T/E) / non-ribosomal peptide synthetase-like protein (AGM06260); Amycolatopsis orientalis HCCB10007 / 90/93 / SACE_3035 (ErcD); 58/69 / SCO0492 (CchH); 40/51 / SCAB_85471; 55/65
AMYAL_RS 0130215 / WP_020635020 / 69 / MbtH / MbtH-like protein (EME62773); Amycolatopsis decaplanina DSM 44594 / 97/100 / SACE_3036 (ErcE); 71/78 / SCO0489 (CchL); 63/73 / SCAB_85461; 69/85
AMYAL_RS 0130220 / WP_020635021 / 338 / hypothetical protein / hypothetical protein C791_1543 (EMD29880); Amycolatopsis azurea DSM 43854 / 94/97 / SACE_2223; 37/53 / SCO2582; 63/73 / SCAB_59671; 31/47
AMYAL_RS 0130225 / WP_020635022 / 361 / iron ABC transporter substrate-binding protein / iron ABC transporter substrate-binding protein (KFU82307); Amycolatopsis lurida NRRL 2430 / 96/98 / SACE_3034 (ErcC); 42/59 / SCO0494 (CchF); 38/57 / SCAB_85451; 58/70
AMYAL_RS 0130230 / WP_020635023 / 585 / ABC transporter permease / hypothetical protein C791_1545 (EMD29882); Amycolatopsis azurea DSM 43854 / 97/98 / SACE_3037 (ErcF); 70/81 / SCO0493 (CchG); 46/60 / SCAB_85441; 63/77
AMYAL_RS 0130235 / WP_020635024 / 558 / ABC transporter ATPase / multidrug ABC transporter ATPase/permease (EME62777); Amycolatopsis decaplanina DSM 44594 / 95/97 / SACE_3038 (ErcG); 63/76 / SCO0491 (CchI); 38/56 / SCAB_85431; 58/69
AMYAL_RS 0130240 / WP_020635025 / 347 / ABC-type Fe3+-siderophore transport system, permease component / ABC-type Fe3+-siderophore transport system, permease component (AIG77011); Amycolatopsis japonica / 97/98 / SACE_0411; 43/65 / SCO0497 (CchC); 50/65 / SCAB_85481; 52/66
AMYAL_RS 0130245 / WP_020635026 / 360 / iron complex transport system, permease component / ABC-type enterobactin transport system, permease component (EME62779); Amycolatopsis decaplanina DSM 44594 / 95/96 / SACE_0412; 44/62 / SCO0496 (CchD); 44/59 / SCAB_85491; 46/63
*Similarity/identity