BIO 111
Ch 5 6th Ed
- The Cell Membrane and Cell Function
- Cell membrane recognize self
- Organ rejection
- Name tags of proteins, carbs and lipids
- cytoskeleton
- cell’s interior scaffolding
- protein fibers
- cytoskeleton + cell membrane
- cells framework
- organizes interior of cell
- distinguishes one cell from another
- The Structure of a Cell Membrane
- Lipid molecules attached to phosphates groups
- Phospholipids – hydrophilic
- Lipid end – hydrophobic
- Phospholipids bilayer – sandwich-like layering repels both charged and uncharged particles
- Proteins- embedded
- Passageway for water soluable molecules and ions
- Also act as carriers
- fluid mosaic – everything moves within the layer
- receptors – glycoproteins on or within the membrane surface
- ratios of proteins to phospholipids reflect health. Upset = sickness
- multiple sclerosis – not enough fat in cell membranes (cells that are supposed to create mylin
- TaySachs – EXCESS lipid in cell membrane (cells that build TOO MUCH mylin)
- Diffusion: Passive Flow Across a Membrane
- Acqueous solution – homogeneous mixture of solute + solvent (water)
- Concentration- how much solute is in the solvent
- Diffusion moves substances from high to low concentration
- Membranes are selectively permeable
- Oxygen, water and carbon dioxide freely cross membranes by diffusion
- Heat increases rate of diffusion
- Concentration gradient- differences in concentration from low to high or vice-versa
- other types of gradients:
- ph
- electrical
- pressure
- ions
- dynamic equilibrium
- equal movement back and forth
- Osmosis is the movement of water
- Same as diffusion, but across a membrane
- Controlled by solute concentration inside and outside the cell
- Solutes can’t move, so water does
- Tonicity
- isotonic – solutes inside and outside have the same concentration
- hypotonic – hypertonic
- relative terms
- can refer to inside or outside of cell
- Red blood cell example – pg 70
- Contractile vacuole – organ that regulates water movement in single cell critters.
- Turgor pressure – how plant cells regulate how much water can flow into cell – water forced against the cell wall
- Transport Proteins
- Channels Allow Passive Movement at a faster Rate
- Forms an opening (tube) for solutes to pass through
- Charge and size regulate what passes
- FAST – 100 million ions or molecules/second
- Form of facilitated diffusion
- Carriers move cargo from one side to the other
- Carrier protein binds to ion or molecule
- Protein changes shape the forces particle to other side of membrane
- Can be passive (no ATP- therefore another facilitated diffusion) or active (use ATP)
- passive facilited 100-1000/second
- active – can move against the gradient
- Pumps use energy to move molecules or ions against a gradient
- Sodium-potassium pump – see animation
- Cotransport
- see diagram (figure 5.11) pg 72
- creates gradient
- gradient used to transport substance
- symporter molecule responds to gradient movement
- Vesicles perform mass movements by packaging substances
- Exocytosis- fluids and large particles out of cell
- Ex – sperm – releases enzymes that penetrate egg
- Endocytosis- capture large molecules and fluid on surface and moves into cell
- pinocytosis – just water or solutes and water
- phagocytosis – captures and destroys debris, small organisms, bacteria
- endosome – phagocytic vesicle fuses with a lysosome
- receptor mediated endocytosis
- receptor protein binds to ligand, membrane indents, pulling ligand into the cell
- Ex. Liver cells take in cholesterol this way
- Trancytosis- combination of endo and exocytosis
- moves particles from one side of the cell to another
- The Cytoskeleton
- Tubules and filaments guide organelle movement, provide shape and link molecules
- Compare micro tubules, aned both filament – Figure 5.14 pg 75
- Microtubules are composed of tubulin
- Composed of:tubulin – protein dimmers (pairs)
- Length adjustable - + or – molecules of tubulin
- Number and arrangement changes based on cell cycle (spindles)
- Cancer treatments – some effect tubule assembly
- Another function: locomotion
- cilia
- 9 microtubule pairs around a central pair (9+2) pattern
- Dynein – connects inner and outer proteins (wheel-like) 5.16 pg 76
- Bad dynein arrangement leads to disease
- Wave-like motion
- Clean respiratory track, move egg down fallopian tube, single celled critters swim
- flagella
- 9+2 arrangement but longer
- Whiplike – propulsion
- Sperm cells – humans 1, cycad tree thousands
- Another function: trackway in cell
- moves organelles and proteins within
- squid color changes – rearrange pigment
- Microfilaments are composed of actin
- Long rod of protein actin
- Not hollow
- Smaller than microtubules
- Function:
- strength to cell (withstand stretching and bending)
- anchor cells together
- muscle contractions
- actin and myosin interact in a sliding filament
- Intermediate filaments provide scaffolding
- Size – between previous two
- Made of many different proteins
- Dimmers intwined into coiled rods
- Abundant in skin cells and nerve cells
- Maintain cell’s shape
- Inner framework of cell
- Epidermolysis bullosa
- intermediate filaments abnormal – skin blisters easily
- Cell Signaling and Response
- Cell to cell communication
- Animal cell junction types depend on function (see figure5.19 pg 80)
- Tight junction
- membranes fuse cells at a single point (like belt)
- create sheets-
- like in human digestive tract
- blood vessels (capillaries) in brain (400 mile-blood brain barrier)
- lipids chemicals soluble – heroin, valium, nicotine, cocaine alcohol
- oxygen passes
- water soluble – different pathways
- desmosome
- links intermediate filaments in adjacent cells
- hold skin cells in place (cell to extracellular matrix)
- gap junction
- links cytoplasm fo adjacent cells (ion exchange)
- heart muscle cells
- muscle cells in digestive track
- Cell Walls Add structure and allow interaction
- Around cells of bacteria, arhaea, fungi, algae and plants
- Provide chape and volume AND interact
- Composition depends on function of cell, surrounding or life cycle of cell
- Plants – mostly cellulose and pectin (like glue)
- CW is layers. Oldest layer is most outside layer
- where cell walls meet: middle lamella
- plasmodesmata – link plant cells (tunnels to cells)
- Cellular Adhesion directs cell movement
- Adhesion – cells stick together
- Cellular Adhesion molecules (CAM’s) (SEE pg 82 Figure 5.21)
- help guide white blood cells to inflammation site
- Know sequence
- Signal Transduction mediates messages
- Signal Transduction – how cells get “messages” from outside the cell
- NO and cyclic AMP (cAMP) used in plants, fungi and animal
- cell membrane interacts with chemicals
- figure 5.22 pg 83