Text S1. Supplemental Materials and Methods
Text S1. Supplemental Materials and Methods
Gene deletion in C. crescentus. We used a gene-deletion method described previously (1). Briefly, the MultiSite Gateway vectors pUHR-0217 and pDHR-0217 were constructed by amplifying 600 bp-upstream and -downstream regions of CcscsB (CC0217) using the primers specified in Table S2 and cloning the PCR products into pDONR221 P1-P4 and pDONR221 P3-P2, respectively by BP recombination (Invitrogen) (Table S2). pThe pKO-0217 plasmid was then constructed with MultiSite Gateway Pro three fragment recombination between pUHR-0217, pDHR-0217, pE2-FRT-tet, and pNTPS-DEST by LR recombination (Invitrogen) (Table S2). The recombinant (pKO-0217) was selected by plating the transformed DH5α on NZ supplemented with spectinomycin and tetracycline. pUHR-1879, pDHR-1879, and pKO-1879 were constructed in the same way above by amplifying 600 bp-upstream and -downstream regions of CcscsC (CC1879). CcscsB (CC0217) in C. crescentus was deleted through a two-step homologous recombination method using pKO-0217 constructed in the presence of a complementing plasmid (pSC161) (2). The deletions were verified using the primer pairs EUHR_F - tetUM13F_R and EUHR_R - tetDM13R_F (Table S2).
Cloning of expression plasmids. Except the cases with a specific description of a template below, DNA amplification was performed using purified genomic DNAs of E. coli K-12, C. crescentus CB15N, and S. typhimurium LT2 (kindly donated by the laboratory of Michael Starnbach at Harvard Medical School). pLAC-YC-9a was constructed by cloning of a KpnI and NsiI-digested fragment of pTrc99a into pLAC-YC previously digested with KpnI and NsiI. The right construct was selected based on the ampicillin sensitivity and the chloramphenicol resistance. pFK074 was constructed by amplifying EcdsbC using primers DsbCTagF and DsbCTagB and cloning into pET-24b previously digested with NdeI and XhoI. pSC27 was constructed by amplifying CcscsB (CC0217) using primers CcscsB_F and CcscsB_Myc-R and cloning into pBAD22 previously digested with KpnI and XbaI. pSC103 was constructed constructed by amplifying CcprxL (CC0220) using primers CC0220 (EI) F and CC0220 Flag (HIII) R and cloning into pLAC-YC-9a previously digested with EcoRI and HindIII. pRD03 constructed by amplifying CcscsC (CC1879) using primers CC1879 (NcoI)F and CC1879FLAG3(HIII)R and cloning into pDSW204 previously digested with NcoI and HindIII. The same pDSW204 digest was used for cloning of pSC105, pSC106, and pSC112 of which each insert DNA [ss-CcscsC, ss-StscsC, and Ccpprx (CC1673)] was amplified using primer pairs CC1879 noSS (NcoI) F- CC1879 His (HIII) R, StScsC noSS (NcoI) F- StScsC His (HIII) R, and CC1673 (NcoI) F- CC1673 Flag (HIII) R, respectively. pSC127 and pSC129 were constructed by cloning of a NsiI-HindIII-digested fragments of pSC112 and pRD03, respectively, into pLAC-YC previously digested with Nsi-HindIII. pSC108 and pSC182 were constructed by amplifying CcscsC (CC1879) using primers CC1879 noSS (NcoI) F and CC1879 His (HIII) R and cloning into pCSF119 and pET28a, respectively, previously digested with NcoI and HindIII. pSC169 was constructed by amplifying Ccpprx (CC1673) using primers CC1673 noSS (NcoI) F and CC1673 His (HIII) R and cloning into pET28a previously digested with NcoI and HindIII. pSC170 was constructed by amplifying CcprxL (CC0220) using primers CC220 ssx (NdeI)F and CC220 ssx (HindIII)R and cloning into pET28a previously digested with NdeI and HindIII. pSC209 was constructed by amplifying CctlpA (CC2210) using primers CC2210 (NheI)F and CC2210flag (HindIII)R and cloning into pBAD43 previously digested with NheI and HindIII.
Because C-terminal FLAG tag fusion is close to the putative resolving cysteine in CcPrxL and it may affect the redox activity of CcPrxL, we generated N-terminal FLAG following its own signal sequence. The region encoding the signal sequence and a FLAG was amplified using primers CC220 (NdeI)F and CC220 ss flag R. The other region encoding a FLAG and mature PrxL was amplified CC220 flag prxL(BamHI)F and CC220 ssx prxL (XbaI)R. The two PCR fragments were joined by overlap extension and amplification using primers CC220 (NdeI)F and CC220 ssx prxL (XbaI)R. After NdeI and XbaI digestion, it was cloned into pBVMCS-2 previously digested with NdeI and XbaI, generating pSC171. The NdeI and XbaI-digested pBVMCS-2 was used to construct pSC159 by amplifying Ccpprx (CC1673) using primers CC1673 (NdeI)F and Flag(XbaI)R, and a template pSC112. pSC160 was constructed by amplifying CcscsC (CC1879) using primers CC1879 (NdeI)F and CC1879 Flag(EcoRI)R and a template pRD03, and cloning into pBVMCS-2 previously digested with NdeI and EcoRI. pSC161 and pSC166 were constructed by amplifying CcscsB (CC0217) using primers CC217’(NdeI)F and CC217’cMyc(KpnI)R and cloning into pRXMCS-6 and pRVMCS-2, respectively, previously digested with NdeI and KpnI. The NdeI and KpnI-digested pRVMCS-2 was used to construct pSC163 by amplifying RcccdA using primers ccda(NdeI)F and ccda cMyc(KpnI)R and a template pFK169. pSC164 was constructed by amplifying CcrnaI (CC0030) using primers CC30(NdeI)F and CC30 cMyc(KpnI)R and cloning into pRVMCS-6 previously digested with NdeI and KpnI.
Expression and purification of PprX, CcPrxL, CcScsC, StScsC, and EcDsbC. To achieve good yields of protein expressions and homogeneous protein preparations, we designed signal sequence-less PprX and CcPrxL for the cytoplasmic expression in E. coli. The 22th Ala residue in PprX and the 25th Arg residue in CcPrxL are the starting ones for those open reading frames. His tag was fused to PprX at the C-terminus. His tag remained since the C-terminal region in a structure of a homologous protein to PprX is far from the two cysteines and appears to be flexible, so we assumed that it would not interfere with the activity of PprX. His tag and a thrombin cleavage site (the original site in pET28a, Novagen) was fused to CcPrxL at the N-terminus since the putative resolving cysteine is at the very end of C-terminus, C-terminal His-tag may affect the reactivity of PrxL. His tag of PrxL was removed by thrombin treatment after purification. The high purity of the two proteins were checked (data not shown), and about 1 ml of 0.56 mM PprX and about 1 ml of 1.1 mM PrxL were obtained. As a control, we prepared E. coli BCP-type Prx (3).
CcScsC-His and StScsC-His without their signal sequences were expressed from pSC105 (or pSC182), and pSC106, respectively. They were purified using Ni-affinity chromatography following the standard manufacturer’s protocol (Novagen). EcDsbC-His was expressed from pFK074 and purified as described previously (4).
Prediction of a substrate of CcScsC in C. crescentus. We searched for the known substrates of E. coli DsbC in C. crescentus and found a periplasmic ribonuclease (RNase) (CC0030: RNase I) which is homologous to E. coli RNase I, a protein with one non-consecutive disulfide (5, 6). However, it is difficult to predict whether CcRNase I has (a) non-consecutive disulfide bond(s) from the sequence alignment of them since the cysteines are not conserved at the same positions (not shown). So, we searched for another homologous RNase I’s of which structures were solved. We found that a tobacco RNase which has non-consecutive disulfide bonds and two cysteines for one non-consecutive disulfide bond are conserved in CcRNase I
Gel filtration analysis. To estimate molecular weight of CcScsC and StScsC, purified CcScsC and StScsC were applied to gel filtration chromatography using Superdex 200 (GE Healthcare). Gel phase distribution coefficients (Kav) were used to estimate molecular weight of the proteins. Kav values were obtained from the elution volumes of the proteins including molecular-weight standard proteins, the total bed volume of the column (24 ml), and the void volume (8.7 ml). After linear regression of standard proteins, molecular weights of CcScsC and StScsC were estimated. To confirm the differences of oligomeric states of CcScsC and StScsC, they were co-injected with EcDsbC which is a homodimeric control with a similar monomeric molecular weight with CcScsC and StScsC.
FOX assay. To measure thiol-peroxidase activity of CcPrxL, the FOX (ferrous oxidation of xylenol) assay was employed using DTT as a non-physiological electron donor to reduce different peroxide substrates. The enzymatic activity was measured by monitoring the disappearance of peroxide (7). The activity was measured using H2O2, tbutyl hydroperoxide, and cumene hydroperoxide as the oxidizing substrates for 120 minutes. 60 μM of each peroxide, 22 μM CcPrxL, and 100 μM DTT were added to the reaction buffer containing 25 mM potassium phosphate (pH7.0) at room temperature.
Amino acid sequences of DsbD, ScsB, and CcdA used to generate TABLE 1, Fig. 2, and Fig. S1E.
>gi|83594388|ref|YP_428140.1| suppressor for copper-sensitivity B [Rhodospirillum rubrum ATCC 11170] ScsB
MSRFLLALCLFLGLCLPAQAQGNDPGASAWVEKSHSRMRLISAVSAAGKAEEIALGLQVELAPHWKIYWR
SPGDAGYPPSFDWTGSLNLAGATPRWPVPTRFSVLGIETVGYEHQVILPLMARLERPGDPLILRLAVDYL
ICAEVCVPDRGDFTLIVPPGPSGASPQAQALARFLAQVPGPSPSHGITVDEAVIETGGVVRVAVRADPPL
AAPDLFVEAPAGMAFARPQVTVEEGGGRAVLRAGVVGGDALIGQSLPVLVADGVRGVEAGVDTRRALAPP
AGAGDPRPTALGGESGGETGAPGAPTGPLAFLAMVSVALLGGLILNLMPCVLPVLSLKILGLVGHGGGAP
RAARLSFLATSAGIIASFLVLALAAIGLKTAGMAVGWGIQFQQPLFLSGMVVLLALFAANLWGLFEIGLP
GAVSGLGGQGRAGSSLSGAFATGAFATVLATPCSAPFLGTAVGFALARGPFEILAIFLALGLGMALPYLL
VALRPQIATRLPRPGRWMLRLKAVLGAVLCLTALWLLSVLAVQIGAVWAAVIGGLLAVALGVRALRPISR
LSGGVAALLGIAALALPALPLPALSLPMAAPSQALEETAWGRWSPEAVARLVAEGKTVLVDVTAQWCVTC
KVNKATVLDAAPTRALLDSGRLSGLRADWTRPDPAIAAYLASFGRFGIPFNAVYGPGAPQGIALPELLSE
NALLEAVARAAGPSS
>gi|49474352|ref|YP_032394.1| cytochrome c-type biogenesis protein [Bartonella quintana str. Toulouse] CcdA
MIVEISTVGVFFAGALSFLSPCVLPLVPPYLCYMAGIGIDDFRSEKHKEKVFIRLALLSSVIAFVLGFTT
VFVALGASASTIGKFIAYYREWFVLTAGIIIIIFGLNFLGLFKVAFLFREARFQTHKTPAGPLGAYVIGL
AFAFGWTPCIGPILGPVITLAGTKETVGEGAILLGVYALGLGVPFVIAALFSSSFMRFLGAFCIHLGKVE
KIIGIFLIVTGISFLTGSMQSLSFWFLEHYPSLSQVEGIHWHDIMIFLGFSR
>gi|23500328|ref|NP_699768.1| ccdA gene product [Brucella suis 1330] CcdA
MKEVLVLDISYMTAAGAGALSFLSPCVLPLVPPYLCYMAGVSVEDFRADASKATAKPSARRSLGFAALCF
VLGFTTVFVALGAGASSIGAFLRTWQQQIAIVAGVVIILMGLNFLGLLRLSFLSREARFQARNAPASPLG
AYIMGLAFAFGWTPCIGPVLGPILTLAGGSSTVGEGALLLATYSLGLGIPFLIAALFSGAFMRFLSRFRV
HLGKVEKAMGVLLIIAGVVFLTGGMQSFSFWLLENFPALGRLG
>gi|154245023|ref|YP_001415981.1| cytochrome c biogenesis protein transmembrane subunit [Xanthobacter autotrophicus Py2] DsbD
MVANPPRSPLSPLRRALATLVVVVLWATGAAPVLAQGGILKADEAFTVSATRATDGTLSLSWRIAPGTYL
YRESLKAGKDGTAVPLTLPPGEEKDDPNFGPVDIYHRSVTASAPALPETGALELSFQGCAEQGVCYPAVT
RQIDLATLTITAARPRVEASPVDQWGTAAPPPKPAAAPGTPAQPAALDALFRRDLLPLLGAFFGLGLLLA
FTPCVFPMLPILSGILAGGGAKPTPARGLVLSGSYGLAMAAAYGVLGMGAAWTGANLQAALQTPWAIGLM
AAVFAVLAGAMVGLFDLAIPSALAARLPSGGSGSIAGAAGLGFASALVVGPCVTPPLAAAMLYAAQTGDA
ARGASALFMLGLGMAAPLVAVGAFGAQILPRSGRWLLAVKQGCGVLFLAIAAWLAARVLPDSAGLALYGL
LAIGLGVFAGGFDALASDADAGRRIGKTAGIAAVAAGLLLLVGAAGGASDPLRPLAFLAAPARPAAAAER
EVRVSSPPALARALAAAGQHGRPTLVSFTADWCTVCKSNETTMAEPAVAARLSGLERIAVDVTRYGDGAR
ALMGRYEVIGPPTLFLLDPDGRELPGSRRVGALTLADIDRLATQAGL
>gi|154245472|ref|YP_001416430.1| cytochrome c biogenesis protein transmembrane subunit [Xanthobacter autotrophicus Py2] CcdA1
MSLDIGLPGAFLAGLLSFASPCVLPLVPAYLGFLTGTVAGHEGEAAPRRRVIAASVLFVLGFSTVFILLG
ATASTLGQILMRHVEALTLVSGALLVLFGLHMTGLVRIPLLYRQAKIDAAATPAGLVGPYLIGLAFGFGW
SPCVGPVLAAILMVAGAEASVGKGMALLAVYAAGIGIPFLLAAAFTGPFLAWAASARRRLGLIEKVAGAL
LILTGLAFMTGFMPEVAQWLMDQVPALGTIG
>gi|154247709|ref|YP_001418667.1| cytochrome c biogenesis protein transmembrane subunit [Xanthobacter autotrophicus Py2] CcdA2
MADVTLPAAFLAGLVSFVSPCVLPLVPPYLCFLGGSTLDDLAGDEPVKEVGRRAMVGAVLFVAGFSVVFV
MLGAGASAIGDLLRAHLDVLSIIAGLAIIVMGLNFLGVFRIHLLHRTARAHVHEPTGIWGAFVMGLAFAF
GWTPCLGPVLGAILAVAGSEATVSKGAGLLAIYSLGLGVPFLLAAFAVRPFLKSLVHMRKILPVVEKAMG
ALLVLTGIAFMSGWIAHASYWLIETFPALSTLG
>gi|154246660|ref|YP_001417618.1| cytochrome c biogenesis protein transmembrane subunit [Xanthobacter autotrophicus Py2] CcdA3
MALESLAFGSFALAFMAGVLSVLSPCVVPLLPIVLGAAAAEHRLAPVALAAGVAISFVTIGLFVATIGYA
IGLEGDVFRILAALLMVGIGTVLAVPQLQTRLAAAGGPVSDLAARRLDGFSRAGIGGQFAVGLLLGAVWS
PCVGPTLGAASVLAAQGQSLPQVAGVMALFGLGAGLPLALLGTLFRPALARWRGRLLGAGKGAKAVLGLV
LVLVGMMILTGTDKRLESALVAASPAWLTTLTTRF
>gi|58617590|ref|YP_196789.1| thiol:disulfide interchange protein dsbD precursor [Ehrlichia ruminantium str. Gardel] ScsB(no_alpha)
MDLIYVLACSLIGGIILNCMPCVFPVLSLKIMSILKNAQHSKKSLIKADGIAYTCGVMVSMLILSVLLLV
LRYFGYVVGWGYHMQSPVLITGLMYIMFLMGLSFSGFYYIPFIFPNITSGYTEKEGLVGSFIVGMISTLI
ATPCTAPFMVSAIVFALNQPWLYSLLIFQALGFGVALPYLVLSCFPQLLSILPKPGEWMKVLQQFLAFPL
YFSAAWLFCILIKQTGIELLFPVLSSVILFVMGIWVMKLINNKKLIGRIVICFVIIIFATSPLYFHKVKE
SIIRSKTANMEVLEFSETTLTQLLDQKKNVLLSVGADWCLTCRVNERIFQLDTIQALFVKKGVIYMKGDL
TSENSEMSSYVNKLNKEGIPLYVLYVNGEKVKTLPQMLTEKVVVDTINKYVK
>gi|16124472|ref|NP_419036.1| thio:disulfide interchange protein [Caulobacter crescentus CB15] ScsB
MLGGHALAGPVVNSGHVESELVAQEAGIAPGGTIYVALRQKIAPGWHTYWRNPGDAGEPTKITWTLPEGW
SAGDMVWPTPVKTRLGPLLDYAYKGEVLIPVPITAAANAQPGTTLSLTADVFYLVCEQVCIPEQAKLSLL
LPVVAGTPAPDPKWGAVIGKVLADAPKPAGLKAVFKLEGQALKLAVTGGPLKGADMAGAYFFPYSSKVIE
HAAEQAIERGAEGLTLTLKPGYDFVGGSTPPAELVGVLATKAGAWEVTATAGEALAGASGLGPPPAEAAP
GGALAGGLAGALLFAFLGGLILNLMPCVFPVLSMKAASLAGHAHEAPKARLQGLAFLLGVVATFLALAGA
LLAVRAGGAAVGWGFQLQSPLVIAGLALLMLLVALNMSGIFEIGTSVQGVGAGASAKGGVSGAFFTGALA
VVVAAPCTAPFMAGALGYALTQPPVIALGVFLALALGFAAPFVAVTFIPGALKLLPRPGDWMEVLKKGLA
FPMYGAALWLVWVFAQQAGPIALGQLLVAGVLAAFGAWLYGLAQARRAVGKGSAVSMILGLLAVVGALAL
AAASALSAKPPVAAAEASTPSGPGLTAEAWSPEKVQALQAEGRPILVDFTAAWCVTCQVNEKVALSGPKV
AEAFKAQNAVLLKADWTNRDPVIAKALADFGRVGVPLYLVYPKNGGEPVILPQLLTEGMVIEAIERAGS
>gi|77462684|ref|YP_352188.1| ccdA gene product [Rhodobacter sphaeroides 2.4.1] CcdA
MFGLDLIDAGLLPAMLIALVAGVLSFLSPCVLPIVPPYLAYMGGISMKELNQGARRGPLMAALFFVLGLS
TVFLFLGFTASAFGAFFLSNAETFATLAGVIVMAFGAHFVGVYRIPFLDREMRMDAGDRGGSAFGAYVLG
LAFAFGWTPCIGPQLGAILTLAASEASIARGTTLLAIYAAGLGIPFLLVAAFFPRLTGVMGWMKRHMERI
ERIMGLLLWTIGLLMLTGGFSSFSYWLLETFPALARLG
>gi|121596141|ref|YP_988037.1| cytochrome c biogenesis protein transmembrane subunit [Acidovorax sp. JS42] ScsB
MSCLRLLQRSLHTLFFIAACAVSTGAIGQIQLKTTAAAGSSSVVTPHVRAELVAQAPDGVGPGKPLWLGL
SITHQPEWHTYWKNPGDSGLPTQLEWQLPAGIDAGEVAWPVPKKIPLGNLANYGYVGTVLLPVPMTVSPA
FNPGPLAKEATFQLKASWLVCRQECIPEEGSFTLQLPLRGTTALHAAAFDAAAAAHPRDLPALGGSSAQV
EGDTVLLRVAGLPPALQGQTLEVFPETTGVVETAATPTQQWDGAVWTARLPLSPQRSDGPVPMPVVLAHG
GQGWRAELPVPGGWPAVAALKGMSPALEAALKANAANAAAPAAPAAATPAPLGTWLAALAGALLGGLILN
LMPCVFPVLAIKVVGFARHAQNQRAHRIGGLAYTAGVVLSFLLLGALMLGLRSAGEAVGWGFQLQSPAVV
ASLAALFTLIGLNLAGVFEFGRMLPSSLAGLQARHPVLDAFLTGVLAVAVASPCTAPFMGASLGLTATLP
AGQALSIFAALGLGLALPYLLASWLPAFARALPRPGAWMDTLRRLLAFPMFATVVWLVWVLGQQSGINGA
AALLALLVALSMAVWALTLRGATRAVLAPLSIAACAWLAWLWGPKIIELESAPMAAASAPSGVAGVTSWQ
PWRPGLADELVAQGRPVFVDYTAAWCVTCQYNKNTTLASAAVLQDFATKNVALLRADWTRRDPAITAALA
QLGRSGVPVYVLHAPGKAPVVFSEILGESELRAAVAAL
>gi|121593754|ref|YP_985650.1| cytochrome c biogenesis protein transmembrane subunit [Acidovorax sp. JS42] DsbD
MAFYRRIVAIFLLCCCMVGSAWANGLLPSWGSSERQFLDVAEVFKLDPAQQQGDSFLIAGHIADGYYVYR
HAIKLVDARGHEISLTLPAGTAKHDEFFGDTEIYKGDALNLRFPVTAAGPLTLHWQGCAEAGICYPPQTV
NLALPEVAAGTSTAGNGSTHESPTAAPASQGSVAGATEIAEDQAAAQRLAALGPVWGTLLFFGFGLLLAF
TPCSLPMIPIVSSMVVGSQAKPRRAFFLSLSYVVAMAGTYAVVGVAAGMAGANLQATLQSPWLLGAFAAL
FLVLASSLFGLFELQMPSALVNRVESAGRDRSGGSIPGAAALGFLSALLVGPCMTAPLAGALLYIGQTGS
AVYGGMALFALGLGMGLPLLAIAVFGARVLPRPGAWMDRVRVAFGYVMVGMAVMMLTRFLPGTVGLVLWG
AWVLSVAIGLIAWGQAVAAKHRLAWTLRSGAVFAGLWSVLMLVGAASGGESALQPLAHLRGGSTSAASAE
AGVAFVQAKSVEDVDAHLAQAASRDQWTLIDFYADWCVSCHVIERNVFGDPAVASRLARMQVVRPDVTRN
DATDQALLKRWGVMGPPTLILVGPDGKERRDLRVVGEIDARTFLDHLDKAGS
>gi|107024274|ref|YP_622601.1| protein-disulfide reductase [Burkholderia cenocepacia AU 1054] DsbD
MFNGMALSVRVRALRFLAVLLSFVFVLSGLSAARAADDFLDPSVAFKFSASESPGQVDVRFKIANGYYMY
RERFAFAVKSGQATLGEPQFPAGHVKFDQTFQKNVETYRDEVVVHVPVKQAAGPFELAVTSQGCADEGIC
YPPAEHVMKVDGAALGAASSSGDTAAAGSWFDKVTSADFAQSLLEGHGFFTIVALYFVAGVVLSLLPCSY
PMIPIVSAIIIGQGTRATHARGFALSLTYVVGMALVYTVLGIAAALVGQSLGAWLQNPWVLGAFAVLLTA
FAVSLISGKDIALPERWQNGAAEASSARQGGHFVAVAAMGALSALVVGACMTAPLFAVLAFIAHTGNALL
GGAALFAMGLGLGVPLLVVGVGAGTVLPRAGAWMDGVKVFFGIVLLAAALWIVWPVLAGALKMVLAALWL
LIAAAALGLFTPNAGAASIWRRLGRGVGAALAIWAATLLVGLAAGSTDPVKPLAVLAARTVASGGAATAG
AAAAQDGPAFASVRSSGELDALLKTSGQPVMLDFYADWCVSCKEMEHLTFTDARVQARLAKLHLVRADVT
ANNPDDQALLKRFNLFGPPGIIFFDRNGNEISRVVGYQAADTFLRSLDRAAVPTV
>gi|124266682|ref|YP_001020686.1| ThiO:disulfide interchange protein [Methylibium petroleiphilum PM1] ScsB
MPQPFGPLFVAALASAALLLGGAAHAAAVRTDHVTAELVAERGAVAAGQTLRIGLKLQHIPHWHTYWRNP
GDSGLPTTLSWTLPPGSRMGEIEWPAPERLPIGPLVNYGYEGEVLLPLQYTAPPDARPGDTLRLQAQARW
LVCNDVCIPEQATLDLRLPVAEAAAADNAAPAAHAALFAQAAAAQAGPLSAWTAEVQQAGRDLLLTLEPA
GGDLPADAPEVHVFPYAEQLLEPASHALYRGPRGYALKLKLLEGATVPARLDGIAVAQAAPGASGTAVWG
GPQRSVEFSAPLRPVATITVPAGARAAAEDRGPASLRGSAPVGLLAALGLAFLGGMLLNLMPCVFPVLSI
KLLGLARQEGDARRLRMHALAYGVGVVCSFVALAAALLALRAAGSAVGWGFQLQEPGVVFALALLFFLLG
LNLAGQFEFGLLMPQGLAQWRAQRPAVDAFGSGVLAVVAASPCTAPFMGAALGYAIAQPPAQALGVFAAL
GLGMAWPYVLLVLRPGWRARLPRPGPWMLRLKQGLAFPMFATVVWLLWVLGQQAGIDGSTRALVALVGLA
FGLWLASVWRGVAARAGVTVLLVAVLAWGWPVSERAASPQAGNGTASNAGSPHTAWQAYDEAAIDAHLAQ
GRAVFVDFTAAWCVSCQVNKRLVLHTDETLQAFTRSNVALMRADWTHRDERITAALGRLGRNGVPVYVLM
RPGREPLLLPEILTGGLVREALSTL
>gi|124268599|ref|YP_001022603.1| disulfide reductase [Methylibium petroleiphilum PM1] DsbD
MPPVTPIRFLRTLLLWLTALLPLFAAAAEDFLPPEQAFRFDARVLDGKSIEIGFVVADGYYLYREPFKFE
AQGGGVVLGAPEIPSGTVKFDETFEKDVETYRGRVAIRLPVDAAPASFQLVVTSQGCADQGLCYPPMQSV
ADVRLAGFGGDGSVRVPADGRGLDGALAASRASASMAGASRSGDAAIDHALRSGRFWLVVFAFFVAGLLL
SFTPCVLPMLPILSSLIVGQGGQAVARSRGLLLAGAYSLGMALVYTALGVAAGLAGEGLAAALQNAWVLG
AFSLLLTLLALSMFGAYELQLPAVLRDRLAASSGRLQGGRLAGVFAMGGVSALIVSPCVAAPLAGALLYI
SQTRDVLLGGSALFSLAAGMSVPLLLLGASAGALLPRAGAWMESVKRFFGLLLLAVAWWMLSPVVPAPLA
VAGWGVLLIGAAVFLHAFDRLPEIAGADLRLVKGLGLALAVAGATQIVGAAAGAQDALKPLQPFVVGISP
AAAAALPGNAIAATHDGVSFRRVSTVEELDAAVRGAGRPVMLDFYADWCVSCKEMERFTFTDPAVQARLG
KALLLQADVTRNSESDRALLKRFQLFGPPGIVFFDAGGRELETRVIGFQNAQRFQQSLQSAGL
>gi|59801364|ref|YP_208076.1| thiol:disulfide interchange protein precursor [Neisseria gonorrhoeae FA 1090] DsbD
MRTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKAFVPELTVADDGVNVRFRIADGYYMYQ
AKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQTVHHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYP
PVDTEFDISGNGTYHPQTDEPASAKDRFLQPSSQNGSGALPPPKGDEGGDGRFKLSWDTLNANLLAFFLA
GLGLSFTACMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQQAWV
VLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSALIVGPCVAPPLAFALG
YIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGDWMNAVKYAFGFILLAVAVYLATPHLPYY
LVVALYTLLMLVPAFMLLVNGRRQKRRPKAVAFALGSILLIGGAWFGWQGANGKTTALHHFLTLNPPAEA
GKFSEHGKMFADTAALKAAMDTALKEHPDKPVVLDFYADWCISCKEMAVYTLNQPEVHQAVDMERFFQID
VTANTPEHQALLKEYGLFGPPGVFVVRADGSRSEPLLGFVKADKFIEWYEQNR
>gi|82703796|ref|YP_413362.1| protein-disulfide reductase [Nitrosospira multiformis ATCC 25196] DsbD
MPLNRYSRYSLLFLCLFSTSVFAEEGKTSGFLSGLQQLRSTLGQDQEEQELLPPDEAFKLKVKARDANTL
VAQFEPAKNYYLYKDKVAFKPQTPGTTVENISLPQGEMKSDVTFGEVEVFNEPFEALISLKRTAPSGDKL
TLVATYQGCNEPIGVCYAPISKVIDLTLPVAKAAAGAVANAMSADASAATGSAGERLDATDELFQSKDGS
AAIETESLEIERMFQTGNFWLILTGFFGIGLLLSFTPCVFPMFPILSGIIAKGGQHVTKQRGFILALAYV
LGMAITYAIAGVAAGLSGAMLSATLQNAWVLGAFALIFVTLAFSMFGFYELQLPTFFQSKISEEAGHLKG
GHLTSVFGMGALSALIVGPCVAAPLAGALLYISQTRDVVLGGSALFAMALGMGLPLLLLGASAGALLPKA
GAWMEGVKQSFGVLLLGVAIWLISPVIPAVVHMLLWAALLIVSAIYLHAVDPLRPDASGPQKFLKGIGMI
ALLTGIALLVGVFSGSRDILQPLSKLNISAAGMEGAKKDGLNSNEHLPFQRVKSVAELNQQILQSRNKYV
MLDFYADWCVSCKEMERFTFTDPAVQARLKDVVLLQVDVTAGTPEDMELLKRFKLFGPPAILFMDKEGRQ
VPNVKIIGYQDTPAFLKILNAVLI
>gi|15600038|ref|NP_253532.1| dipZ gene product [Pseudomonas aeruginosa PAO1] DsbD1
MRRLLTLILLLVALPAGAGLFDSRPGASLGGGLDGGGFLKVDQAFKPSVERSDAQHVLLRFVNAEGYYLY
RHRFQFKVEPAQVSLGQVQLPAGKQHHDEYFGDTEVYYNIVDLEIPLNNPARQPYTLVVTYQGCADKGLC
YPPETRRFDIDGGAATPAASDAAGKGPLEHKGKRSLLFFFLAGLTLTFTPCVLPMLPILSGVVLRGRPGG
GRGFVLSLAYVLPMALCFALLGALMGMFGASLNLQAQLQSPWVLVPFAAFFALFAVAMFGFFELRLPGFI
REPLDRLAGDARGGSILGAATLGVLSSLLVSPCVSAPLAASLLYISASGDAWGGGLQLFALGLGMGTPLV
VFGAGGGALLPKSGAWMNGVRNAFGVLLLAVAVWLLERVVSGPVALMLWGMLAGGAGLALGALEFTPKSA
ARRLLQLLGLMFLTYAVAAWIGALQGESDPIHPLGRSVPSIHAGPPSTPGEWQNLTTPAQLDAALAEARQ
AGKPVLLDWYADWCISCKVIERQVLTDPTVQARLPAYRLLRFDITESNPAQRGLLDRYNLFGPPAILFFA
PGGDEWSDLRVIGEIDAAGLAERLRRAATRQ
>gi|15597674|ref|NP_251168.1| unnamed protein product [Pseudomonas aeruginosa PAO1] DsbD2
MRVLILLLMLLLPGLSQAQPGDDLFAPRGATQTDFLPVEKAFRFTWERLDDGQVQLRWQIAPGYYLYQKR
LRFDGLDPALQPQLPPGESHSDEFFGESQVYRQSLELTLPAAAAGQLRLGWQGCADAGLCYPPQSQALDL
GGTGPAAAGTSGEVAEDQGLAGSLQAGNLAWSLLLFFGLGLLLAFAPCSLPMLPILAGLVVGSGAGPRRG
LLLAGSYVLSMALVYAGLGVVAALLGGNLQAWLQQPWLLGSFAALFVFLALPMFGFFELQLPAALRDRLD
GLSRGRKGGSLAGAAALGALSGLLVGPCMTAPLAGALLYIAQTGNALHGGLVLFSLGLGIGMPLLLLVTV
GSRFLPKPGPWMNLVKGVFGFLFLGTAWILLRPLLGEALWIGLGGALLLVLAYAALHTARGLARHAVLFG
AAGCIFGLWGAAMLLGAAAGADDPWRPLQVYAAANRGATPTASAHEAFLTVSQPAELDRQLAAAKAEGQW
VLLDYYADWCVSCRIMEKQVFAKPDVLAALQGVRLLRLDVTADNAASRELLHRYQVPGPPSLIWIGPEGE
ERRARRLTGEVDAGGFLAHWQATRERG
>gi|83645097|ref|YP_433532.1| thiol:disulfide interchange protein [Hahella chejuensis KCTC 2396] DsbD
MPQLLRALFILTLFCCATVRAESNLFGDNNGFLAEPEFLPVEEAYRFTSSVEGDELVLHWAIADGYYLYK
DRTSVTTDAEGVAQGRWTYSSPGEEKEDPYFGMVYVYHDELTIRVPVAAEEGAEVEFKVRYQGCAEAGLC
YPPQTETALFTGTDSDSISPAATTNDSTQSPSPSTGQSGSSTSSSVPGPSKAADVDFTSSTSITDYLVAQ
GLLFSFFILMAVGIGLAFTPCVFPMVPILSSIIVGQKEVTMLHSFVLSLSYIMGMAAVYTALGAVAGHFG
ELGQDANIQAQVQKPWILITFSGLFVLLALSMFGFYELQMPSFLRDKLSGMNTKQQGGEIFSVFIMGLLS
ALVVSPCVSAPLIAALGYAAYLGSAFKGGVALFGLSLGIGLPLLILGTGGAKLLPRAGAWMEHVKHVFGV
ALLGVAIWMLERILPPEATLTLWAVLIGVSAVYMGAFTNVESGWSGWQKLNKGLGLVFFVYAVTLMLGAA
AGANDPLRPLAPYAIKSAVAANGGPAAQALTFVKVTSPEQLDREVAQAAATGKPVMVDFYADWCISCKIM
ERETFVLPEVQSGLSRFHLVKADVTDNDQGNQALLKRYGIFGPPAFVFFNAQGKELESKRFLGEISKNAL
LNHVKDI
>gi|83646279|ref|YP_434714.1| thiol:disulfide interchange protein [Hahella chejuensis KCTC 2396] ScsB1
MVVLTVVPRQWHFILRVCGALLLWVMAGFASAADSATGEHIRVQLVSEWDGVAPGRTHWVGVILEPDPHW
HTYWRNPGDSGEPPTLTWRLPPTIQAGDIQWPLPDEIPVAHLTNYGYSGAHLLMVPLQVGELSQSNADIS
VDISWLVCREDCIPGWATLSMNLPVEAEPSPSEHHPLFEQTRLNLPAQEIISARYEVTDSHLVLAFTAPK
AGPWRALPLEGNVVVHGASQQLIRDGDVTRLLTPRSEYLSAKGQPIAFLLTNGEQGYYLHSEYNAGASVK
ETSQGGIESLSLWLLMLMAFAGGLILNLMPCVLPVLSIKALGLRQGHHTLMHKLAYAFGVWGSFMAFAAI
IVALKSAGSEVGWGFHMQNPIVIGLLSFLFLFIALNLMDLAPVGGRLAGMGQSLTQGDSAQSQFFTGVLA
VLVASPCTAPFMAAAIGVALVSDALMVFIIFTALALGFALPLSLIYFIPALQRRLPRPGPWMNTFRQFLA
FPMLATVLWLAWIYQQQTSAYAHLWLMIALLGLGFWAWSRQHLARLPSWLVAVATLAVMSQPLRLAQTSQ
DASPPQAAQAFSEHKLATLRARGAVVVVNMTADWCITCKVNEQVAFSSGQVQTLLESENVHYLVGDWTNR
NDEIFGYLQRYGRAGVPLYVVYAGMADGQVLPQLLTPELLIRGIEQAKEEIRYESQAVVTGTLGVNAGGQ
CMDAGGAENRRSGARFQRY
>gi|83646079|ref|YP_434514.1| thiol:disulfide interchange protein [Hahella chejuensis KCTC 2396] ScsB2
MQFLMKMMVWLVALIGSLQVLASEQTDDQRLLSISILLESENPAAGGEYELALTMTPKQGWHGYWLNPGD
AGFPTRLSWRLPQGVKIGEAQYPAPSQLLTTDIMNYVYPGEYTLLTLIKIDKGIAAGSSLPIKLDASYLV
CNSTACLPEQQTIETSIIVGDGKASDSRIDTFSQWRQKLPRPLAAKGRFSVDNGKFVLALPLPDSIRSDN
AHIYPIMNNTIVNSARQSFTHDGDTLRMQTNAGDELGELFQGVLVLDNGVALSFEADKADPAALISDRAN
SNGSASKNTWVIGFAAFFGAILGGLLLNIMPCVFPILSLKILSLTQQGSKEEARTGSVAYTSGAILVCVL
LGGVILGLRAVGHQIGWAFQLQSPAVIAALIVLMSAIGFNLAGLFEIGTLTSGSRLQDKKGPMGDFWTGV
LAAFVATPCTGPFMATALGAALVLPTGIAFMIFAGLGFGMALPILLVGFIPALRAHFPKPGAWMTTARRV
LSLPMFITVIGLVWVLMRQANNDYVLSVLAATMLSTVGLWVTGLWQQSQRRGEWWPAIALTLLPFWAVIS
WIPPAHAAAQIEQKHVSEAVVFSQDKLEAALAENDVFLYFTADWCLTCKVNEKTSIDQKQVKDAFEQNSV
VTMVGDWTNGDSEITEFLSRHGRSGVPLYLWYRQGVSKPTVLPQILTPSILVHQAGK
>gi|146309988|ref|YP_001175062.1| thiol:disulfide interchange protein [Enterobacter sp. 638] DsbD
MAQRFFTLILLLCSTSAFAGLFDAPGRSNFIPADQAFVFDFQQNQHDLSLNWQIKDGYYLYRKQVSITPA
NAKVGEPQLPAGEWHEDEFYGKSEIYRHQLVVPVSVNQAATGATVTVTYQGCADAGFCYPPETKIVPLSE
VDTAAQPAAPPSTEKEEDSAELPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTGRALLLAF
IYVQGMALTYTALGIVVAAAGLQFQAALQHPYVLIGLSVVFTLLALSMFGLFTLQLPSSLQTRLTLMSNR
QQGGSAGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLILVTVFGNRLL
PKSGPWMETVKIAFGFVILALPVFLLERVMGDVWGLRLWAILGVAFFGWAFITSLQAQKTWMRIVQIILL
AAALISVRPLQDWAFGTPAAQNQAHLNFIQISNVDELNHALAQAKGKPIMLDLYADWCVACKEFEKYTFS
DPQVQNALKDTVLLQANVTANNAQDKALLKQLRVLGLPTILFFNAQGEEQPGERVTGFMDATAFSAHLRD
RQP
>gi|146313027|ref|YP_001178101.1| cytochrome c biogenesis protein, transmembrane region [Enterobacter sp. 638] ScsB(no_alpha)
MLTAILAAFLGGVILNFMPCVFPVISLKALGIMRHQGDTASARTEGLGFLAGVIFTMMALAAVLLALRAG
GTAVGWGFQLQSPLVISILALIILGAALNLLGVFEVGLSLQRAGEISLGRGAFVRSALTGALAIIVATPC
SAPFMAGAIGYALVQPPAVSLAIFLSLALGFAAPFTLVSLFPAIARRLPRPGAWMDFLKKGLAFPMLGAF
AWLVWVLAQQAGTAALAALLACAVIVSFAAWLYGIAQRRRYQGQSYKILLAFTVAFFAVAIAPLPNVMKA
DEAQTATQRAENVSPVKWSPQNVAEMRGHGKAIFVNFIASWCITCQVNDRTSLSTQAVKQAIARTGTVYM
VADSTSFNADIDDAMNTFGQGGLPLYVVYPADGSAPKVLPQVLTPSIVVTALDRASGKKA
>gi|16131961|ref|NP_418559.1| fused thiol:disulfide interchange protein: activator of DsbC/conserved protein [Escherichia coli str. K-12 substr. MG1655] DsbD
MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQNQHDLNLTWQIKDGYYLYRKQIRITPE
HAKIADVQLPQGVWHEDEFYGKSEIYRDRLTLPVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSE
VVANNAAPQPVSVPQQEQPTAQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLT
FIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLTLMSN
RQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLMLITVFGNRL
LPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSALGVAFFGWAFITSLQAKRGWMRIVQIIL
LAAALVSVRPLQDWAFGATHTAQTQTHLNFTQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYT
FSDPQVQKALADTVLLQANVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHL
RDRQP
>gi|37527984|ref|NP_931329.1| thiol:disulfide interchange protein precursor [Photorhabdus luminescens subsp. laumondii TTO1] DsbD
MIKRTLMLFLLLCSPLLTPAAANALFEQPGQNPYLPVDQAFMFDFQQKGDKLTLDWQIKPGYYLYHKQLH
IEPQQATLGKITLPQGTAHRDEFFGETEVYFQQLIVNVPVTKANNNSNIVVTYQGCAAAGYCYPPETRLV
PLSAVIPSKTTDAISAEPVHKTPESASNDQQHLPFSPLWAILIGIGIAFTPCVLPMYPLISSIILGSQRP
KSLKQIFWLALSYVQGMAVTYTLLGLIVAAAGLQFQAALQHPYVLIGLSVLFILLALSMFGLYSLQLPSA
VQTRLVNWSNQQKNGSLFGVFAMGALAGLICSPCTTAPLSAILLYIAQSGNTVAGGLTLYLYALGMGLPL
IAVTLFGHKLLPRSGPWMQYVKEAFGFIILALPVFLLERVIGDAWGIRLWSLLAVSFLGWGFVLTIRSQN
GWVRVIQLILLILMLIATRPLQDWFWGTTVTQQSQHSLNFRQINNWQELEQIMTQNSHKTVMLDFYADWC
TACKEFEKYTFSDPQVQSQLGDTLLLQANVTNNSPQQKQLLEKLSVRGLPTILFFDSQGKEIPDSRVNGF
MDATRFNEHLQHLPK
>gi|16764472|ref|NP_460087.1| suppression of copper sensitivity protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] ScsB
MMILFRRILFCLLWLWLPVSWAAESGWLRSPDNDHASIRLRADTSANGETRLLLDVKLENGWKTYWRAPG
EGGVAPSIAWKGDMPEVSWFWPTPSRFDVANITTQGYHDEVTFPMIVRGTLPATLRGVLTLSTCSNVCLL
TDYPFSVTPTVQNADFAHDYARAMGKIPLRSGLTDSLDVGYRPGELVVTATRAAGWSSPGLYLDTVDDVD
FAKPRLRVEGDRLQATVPVTDSWGEKAPDLRNKSLTLVLADGAIAQESTQTIGTAPAQTPDNAALPFWQV
VMMALIGGLILNLMPCVLPVLGMKLGSILLVEEKSRSHIRRQFLASVAGIIASFMALAAFMTLLRLSNHA
LAWGVQFQNVWFIGFMALVMLLFSASLFGLFEFRLPSSMTTKLATYGGNGMSGHFWQGAFATLLATPCSA
PFLGTAVAVALTASLPTLWGLFLALGLGMSAPWLLVAIRPGLALRLPRPGRWMNVLRRILGLMMLGSAIW
LATLLLPHFGFTASKSAQDTVQWQPLSEQAIQSALAQHKRVFVDVTADWCITCKVNKYNVLQKEDVQAAL
QQPDVVALRGDWTLPSDAITDFLKTRGQVAVPFNQVYGPGLPEGEALPTLLTRDAVLQTLKKAKGITQ
>gi|16767572|ref|NP_463187.1| thiol:disulfide interchange protein [Salmonella enterica subsp. enterica serovar Typhimurium str. LT2] DsbD
MAQRIFTLILLLCSTSAFAGLFDAPGRSQFVPADRAFVFDFQQNQHDLTLSWQVKEGYYLYRKQISITPT
KADIAAVQLPTGVWHEDEFYGKSEIYRKRLNVPVTVNQAAAGATLTVTYQGCADAGFCYPPETKTVPLSE
VAAAIDATPTPAVTQTSETSKPAAQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGRQRLSTGRAL
LLAFIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLALSMFGLFTLQLPSSLQTRLTL
MSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLMLVTVFG
NRLLPKSGPWMAHVKTAFGFVILALPVFLLERIIGEAWGLRLWSLLGVAFFGWAFITSLQARRAWMRIVQ
IILLAAALISVRPLQDWAFGSPSAQAPAHLNFTAISTVDELNQALAQAKGKPVMLDFYADWCVACKEFEK
YTFSDPRVQQALGDTVLLQANVTANNAQDVALLKHLQVLGLPTILFFNTQGQEQPQSRVTGFMDAATFSA
HLHDRQP
>gi|82779465|ref|YP_405814.1| dipZ gene product [Shigella dysenteriae Sd197] DsbD
MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQNQHDLNLTWQIKDGYYLYRKQIRITPE
HAKIADVQLPQGVWHEDEFYGKSEIYRDRLTLPVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSE
VVANNAAPQPVSVPQQEQPTAQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLT
FIYVQGMALTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLTLMSN
RQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYALGMGLPLMLITVFGNRL
LPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSALGVAFFGGAFITSLQAKRGWMRVVQIIL
LAAALVSVRPLQDWAFGATHTAQTQTHLNFTQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYT
FSDPQVQKALADTVLLQANVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHL
RDRQP
>gi|108809927|ref|YP_653843.1| thiol:disulfide interchange protein [Yersinia pestis Antiqua] DsbD
MAQRFITLILLLCSVLLAPHSAQSSLFGENASFGTKNSQSRFIPVDQAFAFDFHQQGDQLNLSWQIHPGY
YLYRQQIKIVPQQAALGAFTLPEGITHHDEFYGEVEIFKQQLTLKIPITQAAEQASVSVTYQGCAEAGFC
YPPETRVIPLDVVVAASTASGTAAVNSSATVNPPATTQPEGDATPVPSTLPFSPLWALLIGIGIAFTPCV
LPMYPLISAVILGREKPHSQRRILILAVVYVQGMALTYTLLGLVVAAAGLQFQAALQHPYVLIGLSVLFV
LLALSMFGLYSLQLPSSLQTRLTQWSNSQRGGSLAGVFAMGALAGLICSPCTTAPLSAILLYIAQSGNML
AGGGTLYLYALGMGIPLVVVTLFGNKLIPRSGPWMQYVKEAFGFVILALPVFLLERVLGDVWGLRLWSLL
AVAFFGWAFVLSLKAHAGWVRVCQLLLLAALLIVARPLQDWAFNGNTQQNAVKHINFQPVANLPQLQAVL
AQAQGKPVMLDLYADWCVACKEFEKYTFSDDKVQRQLANTLLLQADVTANNAEHATLLKKFNVLGLPTIL
FFDSQGNEITAARVTGFMDAAQFLQHLQNTPAVTK
>gi|90020216|ref|YP_526043.1| unnamed protein product [Saccharophagus degradans 2-40] ScsB
MFVPPCFKRFCGLLKAFKAVRVLCVVVFAICLAAQAGAQSTAFGDHVKVRWLAPDSLIAGQQEVIGFYFE
VDLGWHVYWHNPGDSGAAPRFDIQVQGATLGEVMWPYPTRLPIEHLTNLGYESNVAYLLPITADLQTSEV
VLNVSLEWLVCKVDCIPGFGEMSLRLPITNQSLWLAPDLALRDGYLALVPQQTNSPWQAVKAQAVGDTQL
QLTLHANSTRLAQGDFNSVSVFPVDGEVLTATKPQIKSNAAGLELTFNRVPGAALPTATDFVVVDGNRAW
RLNDIALVNQAVLLPANSQSQTQPQSFWLLLGAAFLGGVILNLMPCVFPVLSIKLFGLVQQSSAHRAKEG
FVYAAGVLCTFTLLGAVLLGLRAGGAALGWGFQLQSPAVILVLITLFWLMALSFSGVFEFGHRLVTLAGS
HRGGAFITGVLAVFVATPCTGPFMGAALGAAVVLPPLSAITLFVGLGAGLAAPFVALCLYSPLLHKLPKP
GPWMETLRQFLAFPLYATVIWLLWVLGRLVGDIGWLVGASLMLSFVVAIWLASTLQWGKLGRSVICLGAV
VAFVFSLSLLAIPSGNKNSGSETSQWNKFDSQAIAQARAQGRAVFIDYTAAWCITCQVNKKLVLDTQPIQ
DSFKKNNVYLVRADWTHRDDAITQSLAQLGRNSVPVYAWYGKGADEVVLLSQILTAGSIDALFEQK
>gi|90020828|ref|YP_526655.1| unnamed protein product [Saccharophagus degradans 2-40] DsbD
MYTSILRLTQATIACLLLTLLSPAFAADGNAAQQLSSDEAFIPSIELTEEGLLVASWQIADGHYMYKNKF
EFKVASGDFVELGNVSFPEGITKNDKYFGTQEVYKKHVTLEVPVGASALGDFSVRVKYQGCAEQGICFAP
KRKTYEFTANSITSLPTSGSAIATAGEISQTGLGASSVVQTEESRLTTLLKEGTILTIIGVFFLGGLALT
FTPCVLPMIPILSSIIVGQKKELKKTESFALSLSYVIGMATTYATLGVILGVAGNNVIAYLQNPYVITTF
AILFVLLSLSMFGLYELQLPSGIQNKLNNLNNKQQGGTYIGTLIMGILSALIVSPCVSAPLAGALVYIST
TQGGALMGGTALFSLGMGMGTPLLIIGTGGAHLIPRAGGWMDGVKAFFGVMLLAVAIWFLDRILPSQVTL
ALWGALLLGSGVFMGGLDFSSQQGWGQFRKTVGFISVLYGTLMWIGAASGATNPLQPLQNLGSTTVVAQS
GQPSVKEKFVKVSTLEELDAELAIARENGQPVLLDFFADWCVSCKIMEHEVFPSPSVAAQLDNFYLIKAD
VTDDSLLLEHYNLFGPPSLLFFDNSGNEMSNFHIRSEIHAKELAPHLESVIQIVQQAQPAAVESLAKN
>gi|90020461|ref|YP_526288.1| unnamed protein product [Saccharophagus degradans 2-40] ScsB(DsbDalpha_fusion)
MNIMTLKLLYRYVCAIFVSVLLLSAASTQAQGFLSSVDDGFLKVYDAYQVQPQTRQTDTTTALNLEWKAA
DGYYLYQHQFKAVARNATEGYKLALQIEPGIMKYDEYLEKELEVFYNRAFMQAEFPNLAPPYELMITSQG
CADAGLCYPPYKHYFMVEADGTILPMEAASFSDATLTTLTLPESAEATLAPKHAEAAPFLPLVMLSAILG
GLILNLMPCVFPVLSLKALSFASSHVTPHKQHAHGWAYTLGAVLSFVGFAVIMLVAREAGEQLGWGFQLT
NPVFVAFMVYLFFFMGLVLSGFVELGGGWMGMGQNLTSGEGIKSSFFTGVLAAVVASPCSGPFMATALGI
AITQHAAIAIIIFACLGFGMALPFLLLSYNPKLTNRLPAPGVWMDTLKQFFAFPLYLTGVWLLWVLGHQV
NSDAAAALVMGAVLIAFSFWLFKQTTQSKTWNVIRLATAIASLVIALGIAIAAKWFAAPTQYEWEPYSAR
DLAAYRKEGTPVFVDLTADWCTTCKVNEFVALNTEEVQNKAEELGIVMMQGDWTNGEEAITQYLEQFGRN
SVPLYLMYPATPDTPPVVLPQFLSKSLVLEAMQKAVP
>gi|119775845|ref|YP_928585.1| thiol:disulfide interchange protein [Shewanella amazonensis SB2B] ScsB
MKIITFWFHILVGCILLACLPAMASDGPDQKIAASLLLESNTPASGTQTSLAVRMSPEAGWHGYWLNPGD
AGFPLQLQWQLPEGVTVGELQYPAPESLLISGMMNYIYHGDYALLAPLQIADDIAAGTAIDVAVELRYLV
CSPTLCLPEQQRLETHFIVGDKGAPKTQQAAFNVWRQAIPKPVAASADFTVRDGLFVLSLPLPETASVTD
NLHFYPEVNGALSNTAPQSFVQKGDRLFMQTQAGEGDVSDFHGVLTLDKGLAIRVKATGQAGGALMFPTE
AGLPAAQEGSLLSLSVVAVLGAVLGGLLLNLMPCVFPILSLKVLSLTHMGDERLARRGAVAYTLGAVLVC
VMLGALILVLRQFGLQVGWAFQLQRPEIIVALILLMMAIGLNLAGLFEMGTLSSGSRLQQQQGPAGDFWT
GVLAAFVATPCTGPFMATALGAALVLPAYVGLMVFAGLGLGMALPFLLVGFVPALRQRMPKPGAWMVTAK
RIFAVPMLLTALGLVWVLMQQTDRQYVFVALLLAMLLGLALWLMGLLQHGGRERPWWPALLAVIIVAGTL
LSVSPGAETQISKAGAETNRPLTFNEKDLSALIMEQDVFVYFSADWCLTCKVNETTAIERQDTQAAFERA
GMAVMLGDWTNGDPQITEFLAKHQRSGVPLYLWYKKGNLQPEVLPQILTSSLLIERAGG
>gi|119773678|ref|YP_926418.1| thiol:disulfide interchange protein [Shewanella amazonensis SB2B] DsbD
MKKIITLLFSCLLLLAPSTHANDIFGKKFDFLKGEPELMPVNEAFVFDAVQDGNRVRVSFVIADGYYLYR
DKLKFEADNATLGEVALPPGKLHNDEYFGEQQVFYSYVEFTVALKEAMDQGTLTLTFMGCAEGKLCFPPT
RVSKQLTAVAPNDGKLSGESAKPVSDGTVSGTSTSAPLTQQDSLAQMLTEGNLGWTLLIFFGLGIGLALT
PCVFPMYPILSGIIVGQGEKLSTGRAFTLSMAYVQGMAITYSLLGLVVASAGMKYQAALQHPAVLIGLAI
LFFVLSLSMFGLYDLKLPSSWQEKMNGLSNNQKGGNLTGVFIMGVISGLVASPCTTAPLSGALVYVAQSG
DLLMGFAALYVLSMGMGLPLLLMGTSGGKLLPRAGAWMEIIKTVFGFLLIAVSVMMIGRIWPGMVSDLLW
ALWGVALAGYLLHQNKKTEFNWKQTVRTVLLMLGLLAAFSYGFQAVMGGLGYQMPTNNTAQQSVKGVEFK
RIKSVEDLNAELAIARFEGKFVMLDLYADWCVACKEFEHITFPDPEVKKRLDNMVLLQADVTKSDAIDVT
LLETYDVLGLPTLLLFDPQSGLRDDLRVTGFMGPKDFAAHLDLLQVH
>gi|215919238|ref|NP_820704.2| thiol:disulfide interchange protein [Coxiella burnetii RSA 493] DsbD
MSLKKPPKGTSFWHGCRNDVRKRAPVVHFIVEMCLDKSMIRRLITLLVLFFLPIYALSEQPVPVDQAFQF
SAMAKDYQTVIGLWNIKPGFYLYRNRIHFSSLKSADYLGEPLWPRVDTVKDYPGIGKLPVYQGQLRISVP
VIKAMNQTLYLKVDYQGCSEKGFCYPPTTKTVAIDLAGNYMQPVAPLAIDIAPGKTAGPKAAPPITQQDR
VTQLLTHKNLFFVLLSFFGFGLLLSFTPCVLPMIPVLSGIIVGQQKYPKRAFGLSLMYVLGMAITYAIAG
VIFGFIGSTVQAAFQNPWLIGLFSLLFIAMALSLFGLYDLQLPQRWQTRLAQASDRQKHGTYLGTALMGA
LSTLILSPCVTPALVGVLGYISHTGNASLGGVALFTMGLGMGAPLLLLGAFSRKWLPKAGPWMTVIKHIL
GVLMLAVAVWLLARILPGTVTMFLWAALAVGSAIALGALSTTQTKMQKIGKTLGLFLLVYGIVLATGGLS
GNTNPLRPISLASLPEKGEGPSFTAVYTLPDVEHQLKMATTKGQPTMIDFYADWCISCKQMEQFTFANQN
VQKALAGFRLLRADVTRNDQGSTVLEKHFGVVAPPTVLFLDPQGKEIPNSRIVGEMSAFRFLKHLENLRS
>gi|53804808|ref|YP_113516.1| DsbD family thiol:disulfide interchange protein [Methylococcus capsulatus str. Bath] DsbD(two_alpha)
MRDAVKRIGLLLILGLVGIPALAVDGRDLLPPERAFPVAARQSGAGVVSLVWDIVDGYYLYRSKFKFASR
TAGVRLGEPVFPEGHKKHDAFFGEVETYRGHIEVRLPLVAESALPESLELEVTVQGCADAGVCFPPYQRR
VEVKTSAISGGGAFTRLAGALRNVGTGVATGDLLPPDQAFRFFAEAPTGEILRLGWQIAPGYYLYREKFR
ISLRDSGGVALGDYTFPRGEPKVDEEFGAVEVFHGEVAVDVPLVRTDAGPRTVTVEAGFQGCAERGVCYP
PMTRTIDLVLPAAAEGKSVSAMAGATLITEQDRIADGLWRGSLWLNLLSFLGLGILLAFTPCIFPMIPIL
SGVIVGHGHAITTRRAFSLSLAYVLAHALAYTCFGVLAALFGANLQVALQNPWAIGVFSGLFVVLALSMF
GFYQLQLPTVLQSRLAALSSRQQAGSLVGAAVMGLLSAGLVGPCVAAPLAGALIYIGRSGDVLLGGLALF
SLGLGMGLPLLAVGTSAGKLLPKAGMWMNTVKSVFGVAMLAVAVSMLERIVPLSLAMLMWALLLIVPAVY
MGALDALPAGASGWRKLWKGLGVAMVAYGVFMLLGVAAGSRDPLQPLRGVAGASATVADAGPPPFVRVAS
LADLEARLAEAAAAGRWVMLDFYADWCVSCEEMERHTFSDPVVKAKLKEMVLLKVDVTENDDEDQALLQR
FGLIGPPATLFFGPDRSERKPFRLVGFTESEEFVHHLERLFDGVR
>gi|21229994|ref|NP_635911.1| c-type cytochrome biogenesis protein [Xanthomonas campestris pv. campestris str. ATCC 33913] ScsB(two_DsbDalpha_fusion)
MKSLSRWIARCAVLVAPLFVALPLLLTAPAAQAVTEADLLPVDQAFALSAKADGRDRIALTWKIAPGYYL
YRHRISVKSGQGFTAGALDLPEGESKHDEFFGQVQTYRTALQASLAGKAAPGQRSAILQVQYQGCADAGV
CYPPQRRELRVDLPDAPTTAPPRESLGALVPRGPSGARLFGAPGQTAGVDALPLPADQAFNFEAIVGDGN
SLLLRFTPAPGYYLYRDRTSLSLEGTSDIRTGLPRWPQGRTHRDEHFGDVVVYFDQAEVTLPLLRDRPDP
ARVTLVATFQGCQSDGICYPPMTRRVRLDLPAGTVSPHNQATATPLLISPLSAGEVAAERAAPGPPADAA
PGPDATAGNPERSRPPHLERNLLAILLLALAGGLVLNLMPCVLPILSLKVLGLAHSGESRNHARSHAIWY
SLGVLVSFAAIGGLVIGLRAAGQAAGWGFQLQQPWFVAALAYLMFAVGLSLSGVFTLGSNLGGIGQSLAA
RNGPLGDFFTGVLACVVASPCIAPFMGTALAYAFTAPAPLAMLVFLTLGLGLALPFLLIGFIPSLARRLP
TPGAWMETLKQVLAFPMYLTAIWLLWVLGKQRGVDALALMLVGATLLALGLWWFERSRWNSHRLGMGLAA
AMLVLAIVPVVAVTRLSLPRATATEGSVTYTPELLDRLRADNRVVFVNMTADWCVTCKANEKNVLSSTDF
RDALRRVDAVYMKGDWTNVDPKISAFLDQHQAVGVPLYVVYGPGAPPAVLPTVLTGAITEDALLRAAR
>gi|108761980|ref|YP_628569.1| hypothetical protein MXAN_0289 [Myxococcus xanthus DK 1622] ScsB
MTHQQSKRLGVVGGIGLLLVAAVAWADLPASAVASSAPDEGDPRIEGALLVDATEVKPGDTFRVGVRFRL
DPGWHVYWKNPGDSGLETEVAWDTPGVTVGPLQWPFPQTFRTPDGFITTHGYDGEAILIADAKAVEGPST
GLLTVSAAVEALVCEVHCIPAELMLTRSIPLGPQTVRDAETAAVFDTELAKVPRPAKDSGPAATVALDAP
QLTAGQDFTGTLTLTAPGGAAVPAVEGDFFVADRIPGVVRVGLASDGPGRFKLKGKAEPDAQASAPDLTG
VLRLGTAATGYQYLEVTAPLAPLVAAGGAAGTPAVVKPPSVKDSIAAVKPVGAAIATPPAAESSMGLGLA
LVFAFFGGALLNLMPCVFPVLALKAYGFTRMVQQEQGRVGAHAAAYAAGIIGTMLLLASVVLAVRAGGAS
VGWGFQFQEPLFVAGVCAVLVAFALNLFGVFNVGLDGTALAGKVDQSHGLMHSAGEGVLAVVLATPCSAP
LLGTAVGFAFAAGPMTVLAVFTALGLGLALPFCVLVLVPGLAKKLPKPGAWMERFKQVLGFALLATTVWL
VWVMGGLAGVDGMARLLAFLVAVGLVTWLYGQGQAQDGVRKLATVAVAAGVLVASGLLTLRFDDQDPVEA
PRVGSTVAQAQPWSADAVNAALAAGQPVFIDFTADWCLTCKFNERTVLSTDEVRDAFKKHQVAFFIADWT
RRDASITTKLAEHGRAGVPMYLVLSPGAPDKPEVLPELLTPGVVTDAVTRAAACAPSKLGNGANVLCAAG
FPSH
>gi|108759425|ref|YP_633155.1| thiol:disulfide interchange protein DsbD [Myxococcus xanthus DK 1622] DsbD(no_alpha)
MPLQRSSPWPPACVYKGGLGARSHARMDAKKLGVMAVLAGVAVAVVPWLLPTGPNANLDAARFLEAGSLA
VGAAVVFAGGLLTALTPCVYPLIPITVSVFGARKTEGRGRALLLTSSYIVGMGVVFSALGVLAAKTGQAF
GSMLGHPAVVTGLAVFLLLLATSMFGAFELALPSSFQTKLNAVGGAGVAGAFLMGSVSGFLAAPCTGPVL
TGLLAFVAKSANTTLGASLLFVYALGIGVPFFLIGVFTVRLPRGGVWMEWVKSVLGIMLVALAFSYLKDA
FPWARDVVKGLGLHVGRVPGAVIAALLVAVGVALGAVHRSFKEGAREFSFKALGVALVVAALVLRGGALD
GGPVGTLWVSLGLAEPARAPAWQWHHVMPAKQATFSPEAFDQVLAQAKAEGRPVLIDFFADWCAACKELD
RDTYPAQQVISESDEGRFLNIKIDATNSEDSLDALLERFGVEGLPTVAFISPEGKVLTQPRVTGFLAPSP
FATEMKKARCTDANAC
>gi|108761509|ref|YP_634287.1| cytochrome c biogenesis protein/thioredoxin [Myxococcus xanthus DK 1622] CcdA-Trx
MSLGLPGIFLAGLLTFLSPCVLPLVPLYLSFLAGVSLSQLREAGSGVRRPWGVALAFSLGLGSVFVALGM
AATAMGGLLAEHRSGLLQFGGLALFLLGLKQLGFIQIPWMDGEVRPLLGRVRRGGSLLGAFLFGAAFALG
WTPCIGPVLGGVLTYTASSTSEPLMGALYLGTYAAGLSVPLLLVAAAAPLALRWMERAKRHLRKFEVATG
VLLAGLGVLLFTDSLELLVPSAEGGEASAPVMERRASSSPAASETACTSEEGGDGTCALPEEGGVPSFIG
PSALARVERPTMVEFVSQSCPVCQRMEPTVALAEQHCSGKGVEVLRLDVGTAAGRRAAVQYGVRGVPTFL
FLDAAGQEVARRVGEQSLTSLWQGLEVIAGGRCAGLLP
>gi|347359854|ref|YP_387797.2| Protein-disulfide reductase [Desulfovibrio alaskensis G20] ScsB
MKTLRISFFSCLFTLLCLLPAFLAARAVELPPAASSAAGTPDTPDPLVTASQAFSVSINGPARRVAVITL
TPAAGYHFYAREAGEAGKPVAIIALAGDEERPVLYPQGTPKPDAFDPSLTVNVHDGPARFYVPLPDDPAV
QTLSWSVSALMCSAANCWPVSREGTTELAGQLQRAEAQPWWGEYARLEQTSRPPSAVRRIDAGMAAVKQH
PADGNEVRLSWNFTPRFFMESLEVRSMGKALLFGLIAGLILNFMPCVLPVVSLKLSTLLTASGIQDQNKR
IHHFRMHNIWFSAGIMCFFLLLAAVLGLAGLAWGSAFQSQTLVAVLVVLVFTLGLSMFGVFNLPVLDLKA
VPAEAQSSPRGQAFFTGMMATLLATPCSGPFLGGVLGWAFMQSPLVLGAVFASVGLGMSSPYLTMAVFPA
LVKHFPRPGSWIGILERLVGFFLMGTTVYLLRILPETLWPSMLVLLWITGLAAWVWGRFSGLEKSFRQRL
CVRVLCAATVMAAAWWLNQPPAPHPGWERFEAARFEQMLGKQPVIVDFTADWCPNCKVLEHTTLHAENLR
RWMKQYNARIVQVDLTEPSVQGESLLKSLGSSSIPVVALFPAGQHSDKPLVLRDIFTTEQMEEALQQAFG
LPATE
>gi|39996424|ref|NP_952375.1| cytochrome C biogenesis protein CcdA [Geobacter sulfurreducens PCA] CcdA
MESTNITFVGAFVAGLLSFLSPCVLPLIPSFITYITGLSFADIQSEHPTHKVRQQTIVHSLLFIAGFTFV
FVLLGASATFIGGFLHEHMNVIRKVGGALIVIFGIHVSGLVPIHLLLGEKRLQVHRKPAGYLGSFLVGLA
FAAGWTPCIGPILASILMVAATEETVTKGILLLFTYSMGLAIPFFLSSLAMHQFLTFFNRFKKHIRILEI
VTGLFLVVVGVMIFTNYLSVLSRYTMKWFGGM
>gi|39998049|ref|NP_954000.1| thiol:disulfide interchange protein [Geobacter sulfurreducens PCA] CcdA
MSFLDNIEQIVATQPFLAFVAVFVGGVLSSASPCVLATIPLVVGFVGGYAAESRKKAFLYSLSFIVGLSL
TFTIFGAAAALLGTMFGTVGGWWYAAVGAVAVAMGLQMMGFYELRLPVLPQFRPKRGGFWGALLLGLFFG
VASSPCATPVLVVILSFAALQGNILYGTLLLFTYALGHCLLMLAAGTFTGFVEAFAASRGVANVSHWAKR
GSGAVIALAGAWFIWRAF
>gi|218562254|ref|YP_002344033.1| thiol:disulphide interchange protein [Campylobacter jejuni subsp. jejuni NCTC 11168] DsbD
MRIFGIILLSFCLCFASILSLNEAFNVKSNSYNNSISIDIELGKDIYLYSNKLKLYINEKDISSLINLPQ
SSTRGNENVYYQKLNLALPNLLLERFAKNTTNLIKLEFQGCSEQGLCYNPQTWYFDLISKKDAFEISKPY
KAQKTDKKTKIESEESSIANFLATDNFFWILLSFFGYGLLLSLTPCILPMIPILSSLIVAKSNAKFSKKY
SFFLSFIYVFFMSLAYAIAGVIASFLGASIQGILQKPIILILFALIFIAFAFAMFGAFRFELPLRFQTFI
HKKSEKGKGVVGIAIMGFLSALIVGPCVAAPLAGALIYIANTGNALLGGSALFIMSFGMGIPLLFIGLGL
GFIKPGFWMEKVKIFFGFVMLAMAIWILSRIIEENYILIAYGILGVFFSVFMGIFEKSFTIISKIKKSIL
ILILAYSLSIFLGGLFGAKNFLNPLNFNTISASKHALSYDYINNFEQLKQEIQTNTKPIMLDFTASWCEN
CKLLDELTFSDERIIQKMQNYKLIKVDVSENNNEQIKTMKEFNVFGPPVLIFFENGKEKLKITGFISADD
LLKKIEP
>gi|15611320|ref|NP_222971.1| cytochrome C-type biogenesis protein [Helicobacter pylori J99] CcdA
MFDNTLINLFETAPLLTSLLAGILTFLSPCVLPLIPAYMSYISQISLEDIKDGKAKRVSVFLKSLMFVVG
FSLVFLGVGMSMAKLIHSFSFSWVNYIAGGIVILFGLHFLGVFRFAFLYKTQSVGLASKSNSMQRFYPFL
LGMSFALGWTPCIGPIFTSIVIMSASKDAYGLMLMVVFVMGLAIPFLLVALMLERALLFLKSLRKYNRAI
EIVSGLVLILMGILIMTNSLESLTNFLQN
>gi|117923561|ref|YP_864178.1| unnamed protein product [Magnetococcus sp. MC-1] CcdA
MPDVTLLTAFGAGFLSFISPCVLPLVPAYLSYMSGISVDEMKAAGAQGNHKVAMHAALHSLAFVLGFSLV
FVGLGATASALGQLMFDYMEYLSKIGGVLIVIFGLHFMGLFRISFLNFEARFNPDRKPPGMWGAFFIGLA
FAFGWTPCVGPILAGILALSAGQDTVHEGIVLLVAYSAGLGLPFILAGLAINRFLVFFKKVRQHMHKVEI
VAGGLLVIIGVLIFFGNMTVISSLLLQIFPGLGELG
>gi|94967211|ref|YP_589259.1| cytochrome c biogenesis transmembrane protein [Candidatus Koribacter versatilis Ellin345] CcdA
MSSLPLPIAVFLAGLLSFLSPCVLPLVPGYVSLISGASVEDLQSGDRKLSRTVLLNSIMFIVGFSVVFIA
LGAVATGIGQFMNIYRRQLMQVAGVVIIIFGLHLIGVFKIKALLADKRLHDMKGSGTAIGAFLIGFAFAF
GWTPCIGPILAGVLAIAGSQDTVVKGVLLLAVYSSGLAVPFLLTSLFVGGFLAFYTKFRRHLHAVEVVSG
VFLVVVGALILTRHFTILSSYLSFLNRFSL
>gi|119962620|ref|YP_948984.1| cytochrome c biogenesis membrane protein [Arthrobacter aurescens TC1] CcdA
MNSPFAETILNGSLLLAVPVALLAGLVSFLSPCVLPLVPGYLGYVTGLTGVDLQKQRRGRMLAGIGLFVL
GFSVIFVLLGGAFGQLGTLLTGPQNAWITQLLGILVVIMGVVFMGGFGWLQRDAKIHAKPPAGLWGAPLL
GLTFGLGWAPCIGPTYSAVQLLSLSGGSSAAKGALLAFVYSLGLGIPFLLIALAVRRGMGVMSFFRKHRL
AIQRIGGGILILLGILMATGVWGTWVTELQYWFQNDVKLPI
>gi|57116750|ref|YP_177735.1| ccdA gene product [Mycobacterium tuberculosis H37Rv] CcdA1
MTGFTEIAAVGPLLVAVGVCLLAGLVSFASPCVVPLVPGYLSYLAAVVGVDEQLPAGVVKPPVAARWRVA
GSAALFVAGFTTVFVLGTVAVLGMTTTLITNQLLLQRVGGVLIVVMGLVFVGFIGALQRQARFTPRQLTS
VAGAPVLGAVFALGWTPCLGPTLTGVITVASATEGASVARGIVLVIAYCLGLGIPFVLLAFGSAWAVAGL
GWLRRHTRAIQIFGGALLIAVGAALVTGVWNDVVSWLRDAFVSDVRLPI
>gi|57117033|ref|YP_177912.1| unnamed protein product [Mycobacterium tuberculosis H37Rv] CcdA2
MNEALIGLAFAAGLVAALNPCGFAMLPAYLLLVVYGQDSAGRTGPLSAVGRAAAATVGMALGFLTVFGIF
GALTISAATAVQRYLPYATVLIGLALIALGGWLLLGRGLTALTPRSLGVRWAPTVRLGSMYGYGISYAVA
SLSCTIGPFLAVTGAGLRGGSVVGSVAIYLAYVAGLTLVVGVLAVAAATASSALADRLRRILPFVNRISG
ALLVVVGLYVGYYGLYELRLIAGVGANPQDAVIAAAGRLQGALAGWVNQHGAWPWAVLLVVLVVGAFAGT
WFRRVRR
>gi|21222860|ref|NP_628639.1| cytochrome biogenesis-like protein [Streptomyces coelicolor A3(2)] CcdA1
MTTLAAEGYNGTVLNGALLVALPIALLGGLVSFFSPCVLPLVPGYLSYVTGVTGTDLGEARRGRMVAGAS
LFVLGFTAVFVSSGALFGYFGSTLQEERGTLTKVLGVLMILMGVFFVGLMPWFTQREFRFHRRPVTGLVG
APLLGALFGIGWTPCIGPTLASVLALSADQGTAGRGAILTVAYCVGLGVPFVLAAVAFRKALGAFGWIKR
HYVWVMRIGGAMMIATGVLLLTGAWDSIVQDMQTWSNGFTVGI
>gi|21224331|ref|NP_630110.1| integral membrane cytochrome biogenesis protein [Streptomyces coelicolor A3(2)] CcdA2
MSEAGLAMAFLGGLLALLSPCSALLLPAFFAYSFTSRTRLMARTGLFYVGLCTTLVPLGVAGSFASRLFY
GHRDTLVTAGGWTVIALGVAQIVGLGFGSRRMARAAGGRRSGSALSVFALGAVYGLAGFCAGPILGSVLT
VAAVDGDPLHGGVLLAVYSLGMAVPMFVLALLWDRFDLGRRRWLRGRTFRAGPLTLHSTTLLGGLFFIAL
GVAFLAFDGTSALPSVLSTDTEFALEERLSDIGAAVSDRVVLLVLAGAAALTALLVLLRPGGRREAQEPP
EPADSGPGEGDSATRSRMRT
>gi|108804654|ref|YP_644591.1| cytochrome c biogenesis protein, transmembrane region [Rubrobacter xylanophilus DSM 9941] CcdA
MVEASYLAAFFGGMLSLLSPCSALLLPAFFAYAFGSRGELAKKTGVFYLGLAATLVPLGTGISTVSALFY
GQRSALITVSGLVLMVLGAAILLGRGFSIGALERLRGRIRGDSAGSVFALGAVYGFAGFCSGPILGAILT
VAAASGGALRGATLLAVYALGMAAPLFLMALLWDRLDLGGRRWLRGGELRLGRLRVHTTNLISGAMFVLL
GVTFIAYEGTSALSGLYESGGATGLAFAAEKRLGSVGSAVPDAALIAAVVPFAVLLAFRALRPRKPEAPV
GERAGEKRPRDPVPPGRRCGRGT
>gi|153932982|ref|YP_001382666.1| cytochrome c-type biogenesis protein CcdA [Clostridium botulinum A str. ATCC 19397] CcdA1
MNYILLFLEGIITFISPCILPMIPIYVSYFAGEDIDNKNYKNRALISSIAFVAGFTFVFTLLGVAAGTVG
VIFNKYMREINIVSGSIMVVFGLNYLGIINVGLLHRSFKINKSAGHKKSSIMSTALFGMIFGFGWTPCVG
PFLGSALMIASNSTNVFMGASMLMIYSLGLGIPFILSALLIDSLKTTFDFIKRNYKTINRISGGLLIAIG
IMMMTGYLNLLLSILTF
>gi|153933016|ref|YP_001384076.1| cytochrome c-type biogenesis protein [Clostridium botulinum A str. ATCC 19397] CcdA2
MNEIITKAVSLMGNNVLLALLISFLGGIISSFSPCILSSLPLIIGYVNKYGRDDKKTAFKYSLFFSLGII
ITFTSLGIISSLVGKFFTSGGKLWYLLLGVVMIFVGLQLIGVIESKNKACKVPKKRKGLLGAFFLGILGG
VLSSPCSTPILIAILAFVGSKGNILLGFLMLLLYSIGHCFVIILSGTSLGFVESLSGSSKANKINNVLKI
ILGIIILAMGLYLIYIGI
>gi|16078856|ref|NP_389676.1| cytochrome c-type biogenesis protein CcdA [Bacillus subtilis subsp. subtilis str. 168] CcdA
MGDVNYFLTFGAGFLSFISPCCLPLYPAFLSYITGVSMDDVKTEKLLLQKRSLFHTLCFLLGFSVIFIAL
GYGTSFIGSLFRDYHDAIRQIGALLIILFGFITLGVFRPEAMMKERRIHFKHKPSGFLGSVLIGMAFAAG
WTPCTGPILAAVITLAGTNPGSAVPYMMLYVLGFAVPFLLLSFFITKLKWIRKNQLFIMKAGGVLMIVIG
VLLFFNWMSLIIILLSDLFGGFTGF
>gi|56419435|ref|YP_146753.1| cytochrome c biogenesis protein [Geobacillus kaustophilus HTA426] CcdA1
MNDINIFLAFGAGLLSFISPCCLPLYPAFLSYITGVSVDEIKKENGMLQKRAILHTFFFLLGFSVIFIAI
GFGTSVIGKLFVDYQDLIRQISALFIVFFGLVILGVFSPSFMMKDKRLVFRNKPSGYFGSILIGIGFAAG
WTPCTGPILVSVIALAATKPSAAMLYMFAYVLGFAVPFFIMSFFIGKLNWIKKYNTVIVRVGGILMVIMG
IMLFFDWMTKIIIFFTDLFGGFTGF
>gi|56419874|ref|YP_147192.1| cytochrome c biogenesis protein [Geobacillus kaustophilus HTA426] CcdA2
MNDLNLFLAFGAGFLSFISPCCLPLYPAFLSYITGVSVSELKEENAMLNRRSLWHTLCFLLGFSLIFISL
GFGTSLLGRLFVDYQDAIRQIGAVVIVALGLVVAGLWKPAFLLKDRRISFRERPSGYVGSVLIGMAFAAG
WTPCMGPILVAVVALAATNPGSGMMYMLAYTLGFAVPFFMLSFFIGKLQWIRRHSASIMKAGGYVMVAMG
VVLYFDWMTKLIAYTTSLFGGFTGF
>gi|56420615|ref|YP_147933.1| cytochrome c biogenesis protein [Geobacillus kaustophilus HTA426] CcdA3
MSVIFAFVAGALSFFSPCIFPLVPAYVAHLTGTPIRENRIRVGKREILMRSLAFIGGFSVIFVLMGATAS
AIGQMLVQYREFIEKLAGLFIIIFGLQMAGILSFRLLMKEKRWDTSTSKAKGWLSSIFVGMAFAAGWTPC
VGLTLSSILLLASSTETLYSGMVLLLVYSLGLGVPFLLLSLAMTKSLQIVKNVNRWLPLLSKVNGWLLVV
LGVLLYTGQMAKISALLSSYSIFSF
>gi|116515707|ref|YP_816070.1| ccdA-1 gene product [Streptococcus pneumoniae D39] CcdA1
MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQASSGKFSISVTSLLRTLAFIAGISFIFI
LLGYGAGFLGDLLYASWFQYLTGAIIILLGLHQMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTF
SFAWTPCVGPVLGSVLALAASGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKK
VGGFLIIVMGFLVLFGNASILSQLFE
>gi|116516271|ref|YP_816366.1| ccdA-2 gene product [Streptococcus pneumoniae D39] CcdA2
METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSFSLFGRKVLWSGLIRTLCFIAGISLIF
FILGFGAGYFGHILYANWFRYVMGAIIIILGLHQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFS
FGWTPCIGPVLSSVLALAASGGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKI
GGFLIVLMGILLLLGQVNVLAGIFE
>gi|51892870|ref|YP_075561.1| cytochrome C biogenesis protein [Symbiobacterium thermophilum IAM 14863] CcdA1
MASPNLFLAFGAGVLSFVSPCVLPIYPSFLSYLTGVSVDQLSEGSRAVRAHVLKHSLAFFAGFSVIYVAL
GLTASALGSFFYSSRSWLPVVGGIFIALMGLSMMGILRVPFLMRDTRRQLASRPEGYLGSALIGLTFAAG
WTPCIGPILGAVLALAATSPGYGGLLLLAYSVGFALPFIGMAYALGSVRSLARHSVAIQRVGGAVMVFMG
ILLATGGLERISAWLLEITGFTGF
>gi|75910027|ref|YP_324323.1| unnamed protein product [Anabaena variabilis ATCC 29413] CcdA
MLETLQTQIYQLEKFANTLVSYQLTHLSLLSIGIIFAAGLLTSLTPCMLSMLPITIGYIGGYEAKSRWQA
AAQSTWFALGLATTLAGLGVVAALVGKVYGQIGIGLPIIVSIIAILMGLNLLEALPLQLPSFGDTNWIPQ
DLPSGVRSYGIGLTFGLVASPCSTPVLASLLGWVANTQDLILGAVLLISYTAGYVTPLILAGTFTASIKK
LLELRRWSGWINPVSGALLVGFGVFSLISRIPLGSF
>gi|37523532|ref|NP_926909.1| thiol-disulfide interchange protein DsbD-like protein [Gloeobacter violaceus PCC 7421] ScsB
MRWLTFVMFWFAVGFAVLTVPVAAIPVRSAHTEAELISEVRSVQPGRPFWVALRLKMDPEWHTYWLNPGD
SGTATTLQWQQPAGIQAGPLRWPYPQRIAAPPLASYGYEGTVYLLSEIQAPADLKPGAKLALRAKAEWLE
CKVECLPGAGTVALELPVQAEATAPSVHADAIAAARGRLPVTIADWKLRAAVQEGQLILRATRPEWFAGE
IGPVTFFPEQDLLIEPAAPQVLEVRPDGFLLKLKRSAVSTATPERIAGVLVAPAGWRGAGSEQALSVRVA
LEPVAAVLSAGAPDLAGLLVAVGFAFLGGMILNLMPCVLPVLSIKVLGFVQEARTGHAWRYGLAFTAGVL
ASFWLLAGGLLLLRAGGEQLGWGFQLQSPPFLVILCAVLFLFGLNLFGIFEVGTSLTRLGGAGPAGGLGG
SFFSGVLATTVATPCTAPFMGSALGYALTQPVWAALLVFTALGLGMASPYLLLSLSPALLRFVPKPGAWM
ETFKQAMGFLLMGTVVWLGWVLGLQAGVSALAALLAALVVLGAAAWALGKWGHMAAPAPTRLTARVLAAL
GIVGALGVALVGVEQQSAASSGPTQATATPSEGIRWEAFSPQRVAALRAAGTPVFIDFTAAWCLSCQVNE
RVAFSSPQVREAFARGGFVMLKADWTKRDAAIAQALAQFGRSGVPLYVLYGPTAGAPPRILPEVLTPDII
VKALQELS
>gi|37523189|ref|NP_926566.1| hypothetical protein gll3620 [Gloeobacter violaceus PCC 7421] CcdA1
MVAVPLGLAFAGGFLTVLSPCILPVLPILVGRSLRSHRFGPVALVIGLVGGFALAGSLLGVTAEWFAGLA
SFLRTAAIALLLALGLAALFPERSERLWARLGGFQPLEPKQDGLWGELLVGSQLGLIWTPCAGPVLGSIL
TLAAVGGQPTGAFGLLLVYGLGAAVPMLVFAYGGRYLSARLLGLRAQGELLQRIAGAAVAASALAILLGW
DVTVQLWLAPLLPTPTL
>gi|37521288|ref|NP_924665.1| c-type cytochrome biogenesis protein [Gloeobacter violaceus PCC 7421] CcdA2
MSEALQLWLYQIEQGAEQLARAQLTQISPTSFLLLYLIGLATSFTPCILSMLPVTIGYIGGYQSRSRFEA
LVQSGWFALGLAVTITGLGLVATIPGMLYGQVGTGWFIAMGALAILMGFNLLGLIPLSLPQWGGFDLAKD
TPQSLKSLATGATFGLVASPCSTPVLIALLAWVSTTGNPWVGAGLLFSYSIGHATPLILAGAFTGALKAM
LSLRRWSGLINQVSGILLIFTGTLAILNILNR
>gi|157414060|ref|YP_001484926.1| putative c-type cytochrome biogenesis protein CcdA [Prochlorococcus marinus str. MIT 9215] CcdA
MQNGLNSPGPFTIFLVFSAGLLTSLGPCSLSLLPVTIAYIGGTENNKFKLISFSGGVVFSLVALGAASGF
LGKIYGQIPSYYTSFVALVAIIMGLNLLGILKFQFPNGPDLKILEDKIPSLIAPFTIGTTFGLASSPCIT
PVLATLLAWVSQAKNPIISIIFLFFFGIGQVTPLIIAGATAENLKKFLELRKYSQIIPTLSGIFLVALGL
LNLFSNWI
>gi|56751199|ref|YP_171900.1| ccdA gene product [Synechococcus elongatus PCC 6301] CcdA
MFDTFARWFEGLETLLFQLSLWAETLVNSQLQHPGLPTFAIVWVAGLATSLTPCTLSMLPVTFAYLGGYS
EGDRAQSLRQSGLFCLGLATTLMGLGLSAAVLGRIYGQIGWGLTLASSLIAIVMGLNLLELLPLRLPGLN
TTSWLQEEWPIDLRAYLLGLTFGLVASPCSTPVLATLLAWVAASGQVQLGAALLGTYALGSVLPLLLAGL
LAASLRQLLALRQWSAWLTPTSGVVLIGFGVLTLLLRLPINA
>gi|113474482|ref|YP_720543.1| unnamed protein product [Trichodesmium erythraeum IMS101] CcdA
METILTFFYETAQYANSLVKTQLTHLSWVSLVTIFVAGLLTSLTPCMLSMLPITIGYIGGYEAKSRIQAT
VQSAWFSLGLATTLAGLGIIASLLGKVYGQVGVGLPIVVSIIAIFMGLNLLEALPLPMPSFDSMGWISQD
LPKGVRSYLLGLTFGLIASPCSTPVLATLLAWVSTTGDLILGGFFLLFYAMGYVTPLILAGAFTANIKKF
LEIRKWSSWITPASGILLVGFGVFSLLSRIIPV
>gi|15606125|ref|NP_213502.1| cytochrome C-type biogenesis protein [Aquifex aeolicus VF5] CcdA
MDVTVISAFLGGLLSFLSPCILPIIPAYLSYISGVGVSDVETQKGKVNWKVFFSALYFVLGFTLVFTGLG
ASATFVGQLLHDYQEWIIRVGSGLVIFFGLHFAGVFLWKHFLKVYIPIGILIPVLYFLKLLSWNEFFNLM
FAYAVVLILYLVKAHEFLYRQLKIEAKAEISYLGAFLMGVVFAFGWSPCIGPVLGSILFLASQQETVAKG
ALLLFVYSIGLGIPFLLAGLLFSLFLNFVRSFSRYFKYVEIAGGVLLVSLGLLLALDKLSLIANITF
>gi|60682503|ref|YP_212647.1| cytochrome C biogenesis-like protein [Bacteroides fragilis NCTC 9343] DsbD
MKKLSFFLLLLLVVFTAQAQIQEPVKFKTELKTLSGAEAEIVFTGTIDAGWHVYSTDLGDGGPISATFNV
EKMSGAEVVGKLTPRGKEVSDFDKLFEMKVRYFEKTAQFIQKIKFTGSDYSIEGYLEYGACNDENCLPPT
QVPFKFSGKAAATAEVSAKETPATPVKEPVATVTDSIVEPTATTVTTAIGSVDLWKPVINDLKKFGEANS
QEDMSWIYIFITGFLGGLLALFTPCVWPIIPMTVSFFLKRSKDKKKGIRDAWTYGASIVVIYVALGLAIT
LIFGASALNALSTNAVFNILFCLMLIVFAASFFGAFELTLPAKWSTAVDSKAEATSGLLSIFLMAFTLSL
VSFSCTGPIIGFLLVQVSTTGSVVAPAIGMLGFAIALALPFTLFALFPSWLKSMPKSGGWMNVIKVTLGF
LELAFALKFLSVADLAYGWRILDRETFLALWIVIFALLGFYLLGKIKFPHDDDDTKVSVSRFFMALVSLA
FAVYMVPGLWGAPLKAVSAFAPPMKTQDFNLYTNEVHAKFDDYDLGMEYARQHNKPVMLDFTGYGCVNCR
KMELAVWTDPKVSSIINNDYVLITLYVDNKTPLTEPVKIMENGTERTLRTVGDKWSYLQRVKFGANAQPF
YVLIDNEGNPLNKSYAYDEDISKYINFLQTGLENYRKEK
>gi|110639841|ref|YP_680051.1| thiol:disulfide interchange protein [Cytophaga hutchinsonii ATCC 33406] DsbD
MKVSIVFLFAFLFLTVQVQAGKNVSIPETELQKLAQHVDNLVKRDSVNEAIIKLLKDSLDGKGHTVAKAQ
TPKVSTVKITVKERGGITQDTSLFLFAIFAFAAGFVSIITPCVFPMVPMTVSFFTNTSSSKKESVRKAII
YGLSIVTIYTLIGVVIAKINGPEFANFLSTHWFPNVLFTLIFLVFALSFFGLFEITLPNNFINKVDKQSD
KGGYVGIFFMAFTIVLVSFSCTGPIVSTILLSAAGGEIVKPIIGMVAYSSAFALPFTLFALFPNWLKSLP
KSGGWLNTVKVSLGFIELALALKFLSIADQVMHWNILNRNTFLLIWILIFFSLGLYLLGVIKFSEHDKGS
IGWPRRSLAILSILFAGYLGTGIAGSPLKKLSGYLPPLEDYTTATDRICGTPKYADFLHLPLGLQGYFDY
KEALACAKQQGKPLFIDFTGHGCVNCREMERNVWADPAVLKHLNEDFVMVALYVDDRTELPENEWYTSKK
DGKVKKSIGKQNHDFQNTRFNFLAQPFYVILDPNTEMELAQPITYEPSIEAYIDFLEEGKANYKKLYK
>gi|146299773|ref|YP_001194364.1| unnamed protein product [Flavobacterium johnsoniae UW101] DsbD1
MKNSILTFLLLLSASMFGQMYNPVKWSASVEKISDKEYILKAKAVIQSGWHLYGQYIEEGGPSPTAFTFK
NTPKKFELVGKTTEEKGHETIDKIFDMKIKYFEDKAFFTQKIKLISDVSSITADVDFMVCDDSNCLPPTT
EELTFKIPADKKLAFAEETPAVKKDSAVAEDVTFVKAVDKPEKTAVNPVKKEESRGLLSIFIIAFLSGFA
ALLTPCVFPMIPMTVSYFTKQSKTKAAGIRNAMIYGFSIVIIYVLLGSIVTAVFGADSLNALSTNVWFNL