Tables (supplementary informations)

TABLE 2. Plasmids used in this study

Name / Properties / Resistance / Source or Reference /
F´8::TnΦ(ptsGop-lacZ) / (5)
pBAD24 / ApR / (3)
pDL804 / Ø(lexA*´::jun) / ApR / (2)
pDM2 / Ø(lexA*´::mtfA) / ApR / this study
pDM3 / Ø(lexA*´::dgsA) / ApR / this study
pGEMâ-T / ApR / Promega
pMM2 / Ø(lexA´::mtfA) / TcR / this study
pMM3 / Ø(lexA´::dgsA) / TcR / this study
pMM4 / Ø(lexA´::mtfAΔaa1-83) / TcR / this study
pMS604 / Ø(lexA´::fos) / TcR / (2)
pSU18 / CmR / (4)
pSU19 / CmR / (4)
pTBY1 / MtfA L40T / ApR / this study
PTBY2 / MtfA L40A / ApR / this study
pTBY3 / MtfA L40E / ApR / this study
pTBY4 / MtfA K51R / ApR / this study
pTBY5 / MtfA A70D / ApR / this study
pTBY6 / MtfA A70S / ApR / this study
pTBY7 / MtfA V77A / ApR / this study
pTBY8 / MtfA Y94S / ApR / this study
pTBY9 / MtfA P95S / ApR / this study
pTBY10 / MtfA G120S / ApR / this study
pTBY11 / MtfA G120S/Q121L / ApR / this study
pTBY12 / MtfA W132Y/P451P / ApR / this study
pTBY13 / MtfA S142F / ApR / this study
PTBY14 / MtfA S142A / ApR / this study
pTBY15 / MtfA N145Q/E190G / ApR / this study
pTBY16 / MtfA N145D / ApR / this study
pTBY17 / MtfA N145Q / ApR / this study
pTBY18 / MtfA I148E / ApR / this study
pTBY19 / MtfA I148Y / ApR / this study
pTBY20 / MtfA H149K / ApR / this study
pTBY21 / MtfA H149Q / ApR / this study
pTBY22 / MtfA E150D / ApR / this study
pTBY23 / MtfA E150Q / ApR / this study
pTBY24 / MtfA H153Q / ApR / this study
pTBY25 / MtfA D156E / ApR / this study
pTBY26 / MtfA D156N/D161E / ApR / this study
pTBY27 / MtfA D156N / ApR / this study
pTBY28 / MtfA G160S / ApR / this study
PTBY29 / MtfA D161E / ApR / this study
pTBY30 / MtfA W177Y / ApR / this study
pTBY31 / MtfA Y205S / ApR / this study
pTBY32 / MtfA A207D/Y205H / ApR / this study
pTBY33 / MtfA A207D / ApR / this study
pTBY34 / MtfA A207S / ApR / this study
pTBY35 / MtfA P210S / ApR / this study
pTBY36 / MtfA E212Q / ApR / this study
pTBY37 / MtfA E212D / ApR / this study
pTBY38 / MtfA F214M / ApR / this study
PTBY39 / MtfA A215D / ApR / this study
pTBY40 / MtfA A215S / ApR / this study
pTBY41 / MtfA V216D / ApR / this study
pTBY42 / MtfA V216L / ApR / this study
pTBY43 / MtfA V216L/L217I / ApR / this study
pTBY44 / MtfA S218A / ApR / this study
pTBY45 / MtfA E219Q / ApR / this study
pTBY46 / MtfA E219D / ApR / this study
pTBY47 / MtfA E219D/F214F / ApR / this study
pTBY48 / MtfA F222M/K189E / ApR / this study
PTBY49 / MtfA F222M / ApR / this study
pTBY50 / MtfA P225S / ApR / this study
pTBY51 / MtfA P233S / ApR / this study
pTBY52 / MtfA Y243S / ApR / this study
pTBY55 / MtfA V77A/S142F / ApR / this study
pTBY57 / MtfAΔ1-131 / ApR / this study
pTBY58 / MtfAΔ1-81 / ApR / this study
pTM30 / ApR / (2)
pTM30mlchis / Mlc-his / ApR / this study
pTM30mlchis* / Mlc*H86R-his / ApR / this study
pTM30mlc-tev / untagged Mlc / ApR / this study
pTMB106 / MtfA D106N / ApR / this study
pTMB109 / MtfA G109S / ApR / this study
pTMB112 / MtfA H112K / ApR / this study
pTMB3Ala / MtfA H149A/E150A/H153A / ApR / this study
pTMB4er / MtfA H149K, E150Q, H153K/E212Q / ApR / this study
pTMByeeI-S / ApR / (2)

TABLE 3. Oligonucleotides used in this study

Primer-Name / Sequence (5´to 3´) /
149A,150A,153A- / AACCAGAAGCATCAAAAGAATCTTGTATATC
149A,150A,153A+ / TTAACCTGATTATTGCTGCAGTCGCTGCTAAGCTGGACACCC
149K,150Q,153K- / GTTACGGGTGTCCAGCTTTTTAGCGACTTGTTTAATAAT
149K,150Q,153K+ / ATTATTAAACAAGTCGCTAAAAAGCTGGACACCCGTAAC
A207D- / TTCAGCAGGATCGCTGTCTGCATAAGCATCAATG
A207D+ / CATTGATGCTTATGCAGACAGCGATCCTGCTGAA
A215D- / ATATTCAGAAAGTACGTCAAAACATTCAGCAGG
A215D+ / CCTGCTGAATGTTTTGACGTACTTTCTGAATAT
A70D- / GTAGGCAAAATAGAAGATCTATCCGGCAGCTTC
A70D+ / GAAGCTGCCGGATAGATCTTCTATTTTGCCTAC
BTH1 / TGTGAAATGCTCGAGAAGTGG
BTH3 / GCCGAAAACAGATCTGTATCATTA
BTH7 / GCAGAAGGTGACCTGGAAAGTAC
BTH8 / GTTAGGTCTCGAGCTTGTG
D106N- / CGTTGGTTATGCACCAGACCGATATCGTTTTCCC
D106N+ / GGGAAAACGATATCGGTCTGGTGCATAACCAACG
E150D- / GTCCAGCTTATGAGCGACATCATGAATAATCAGGTTAAA
E150D+ / TTTAACCTGATTATTCATGATGTCGCTCATAAGCTGGAC
E150Q- / GTCCAGCTTATGAGCGACTTGATGAATAATCAGGTTAAA
E150Q+ / TTTAACCTGATTATTCATCAAGTCGCTCATAAGCTGGAC
G109S- / GAACAATACGTTGGTTATGCACCAGGCTGATATCG
G109S+ / ACGATATCAGCCTGGTGCATAACCAACGTATTGTTC
G120S- / CTTGCTGCCAGCTCTGACTTGACTGAACAATACG
G120S+ / CGTATTGTTCAGTCAAGTCAGAGCTGGCAGCAAG
G160S- / TCCGCTGGCGCGATCGCTGTTACGGGTGTCCAG
G160S+ / CTGGACACCCGTAACAGCGATCGCGCCAGCGGA
H112K- / CAATACGTTGGTTCTTCACCAGACCGATATCGTC
H112K+ / GACGATATCGGTCTGGTGAAGAACCAACGTATTG
H149K- / CAGCTTATGAGCGACTTCTTTAATAATCAGGTTAAAACC
H149K+ / GGTTTTAACCTGATTATTAAAGAAGTCGCTCATAAGCTG
H149Q- / CAGCTTATGAGCGACTTCTTGAATAATCAGGTTAAAACC
H149Q+ / GGTTTTAACCTGATTATTCAAGAAGTCGCTCATAAGCTG
H153K- / GTTACGGGTGTCCAGCTTTTTAGCGACTTCATGAATAAT
H153K+ / ATTATTCATGAAGTCGCTAAAAAGCTGGACACCCGTAAC
H153Q- / GTTACGGGTGTCCAGCTTTTGAGCGACTTCATGAATAAT
H153Q+ / ATTATTCATGAAGTCGCTCAAAAGCTGGACACCCGTAAC
I148E- / ATGAGCGACTTCATGTTCAATCAGGTTAAAACC
I148E+ / GGTTTTAACCTGATTGAACATGAAGTCGCTCAT
I148Y- / ATGAGCGACTTCATGATAAATCAGGTTAAAACC
I148Y+ / GGTTTTAACCTGATTTATCATGAAGTCGCTCAT
K51R- / GTAAAGGAACAAGCCGCCTTTGCTGTAAAAAACG
K51R+ / CGTTTTTTACAGCAAAGGCGGCTTGTTCCTTTAC
L40E- / GTTCGGCAAGAGTGACTTCTTTGCTTTGTTCCTG
L40E+ / CAGGAACAAAGCAAAGAAGTCACTCTTGCCGAAC
L40T- / GTTCGGCAAGAGTGACTGTTTTGCTTTGTTCCTG
L40T+ / CAGGAACAAAGCAAAACAGTCACTCTTGCCGAAC
MlcBamHI / GTATGCGGTGGATCCTGAAAACC
Mlc HindIII / CAGTTAAAAAAGCTTAACCCTGC
Mlc His+ / GCACCACCACCACCACCAC
Mlc His- / GATCGTGGTGGTGGTGGTGGTGCTGCA
Mlc his-tev / CTGCAGCACCACCACCACCAGTGGAGAATTTGTATTT
TCAGGGTGTTGCTGAAAACCAGCC
MlcPX- / GTTAGGTCTCGAGCTTAACCCTGC
MlcPX+ / TGTGAAATGCTGCAGCACCACCAC
MlcXB- / AGTTAGTTAGATCTCAAGCTTAAC
MlcXB+ / TGTGAAATGCTCGAGCACCACCAC
N145D- / GACTTCATGAATAATCAGGTCAAAACCAGAAGCATCAAA
N145D+ / GATGCTCTGGTTTTGACCTGATTATTCATGAAGTCGCT
N145Q- / GACTTCATGAATAATCAGTTGAAAACCAGAAGCATCAAA
N145Q+ / GATGCTTCTGGTTTTCAACTGATTATTCATGAAGTCGCT
P225S- / GAGGAGCAAAAAGTTCTGAGGCGCTAAAGAAATA
P225S+ / TATTTCTTTAGCGCCTCAGAACTTTTTGCTCCTC
P233S- / GAAACGTTGCCACAATGAAGAGAAACGAGGAGC
P233S+ / GCTCCTCGTTTCTCTTCATTGTGGCAACGTTTC
P95S- / GACCACAAATGGCGCAGAATAAATTAAGACTTC
P95S+ / GAAGTCTTAATTTATTCTGCGCCATTTGTGGTC
pDL804- / CCTCAGCAAATCCAGCCCTTC
pDL804+ / CCGGCGCATCACGCGGGATTC
pMS604- / GCAACTTTATCCGCCTCCATCC
pMS604+ / CGCGGGATTCGTCTGTTGCAGG
PTM30-neu / GAAAATACCGCATCAGGCGCC
ptsGO1 / TTGGCACTGAATTATTTTACTCTGTGTAATAAATAAAGGGCG
ptsGO2 / CGCCCTTTATTTATTACACAGAGTAAAATAATTCAGTGCCAA
S142F- / AATCAGGTTAAAACCAGAAGCATCAAAAGAATC
S142F+ / GATTCTTTTGATGCTTCTGGTTTTAACCTGATT
S142F-neu- / AATCAGGTTAAAACCAAAAGCATCAAAAGAATC
S142Fneu+ / GATTCTTTTGATGCTTTTGGTTTTAACCTGATT
S218A- / GCTAAAGAAATATTCAGCAAGTACGGCAAAACA
S218A+ / TGTTTTGCCGTACTTGCTGAATATTTCTTTAGC
TM1,2frg- / AGCAAATGCATTCTTCTGCAGAATTGAGAGAATTCCTCC
TM1,2frg+ / CTCTCAATTCTGCAGAAGAATGC
TM1,2frg+ / GGAGGAATTCTCTCAATTCTGCAGAAGAATGCATTTGCT
V216D- / GAAATATTCAGAAAGGTCGGCAAAACATTCAGC
V216D+ / GCTGAATGTTTTGCCGACCTTTCTGAATATTTC
V77A+neu / CTATTTTGCCTACCCGCTCTGGAGTTAGGACTG
V77A-neu / CAGTCCTAACTCCAGAGCGGGTAGGCAAAATAG
W132Y- / ATCTTGTATATCCAAGTAGTTCAAAACGATTGG
W132Y+ / CCTATCGTTTTGAACTACTTGGATATACAAGAT
W177Y- / CATGAAGATCGTGTTCGTAGCCAGCAACCTCACG
W177Y+ / CGTGAGGTTGCTGGCTACGAACACGATCTTCATG
Y94S- / CCACAAATGGCGCAGGAGAAATTAAGACTTCATG
Y94S+ / CAT GAAGTCCTTAATTTCTCCTGCGCCATTTGTGG
YeeI 10 / CTGGAGTTAGGACTGGAATG
YeeI 11 / AATGAACAACATTCAGGAAG
YeeI 12 / ACCGCATCAGGCGCCTTAGT
YeeI 13 / CTTCATGAATAATCAGGTTA
YeeI14 / GAATCCAGTTCAAAGCCC
YeeID156E- / GCGATCGCCGTTACGGGTTTCCAGCTTATGAGCGACTTC
YeeID156E+ / GAAGTCGCTCATAAGCTGGAAACCCGTAACGGCGATCGC
YeeID156N- / GCGATCGCCGTTACGGGTGTTCAGCTTATGAGCGACTTC
YeeID156N+ / GAAGTCGCTCATAAGCTGAACACCCGTAACGGCGATCGC
YeeIE212D- / GAAAGTACGGCAAAACAGTCAGCAGGATCGCTGGCTGC
YeeIE212D+ / GCAGCCAGCGATCCTGCTGACTGTTTTGCCGTACTTTC
YeeIE212Q- / GAAAGTACGGCAAAACATTGAGCAGGATCGCTGGCTGC
YeeIE212Q+ / GCAGCCAGCGATCCTGCTCAATGTTTTGCCGTACTTTC
YeeIE219D- / GGGGCGCTAAAGAAATAGTCAGAAAGTACGGCAAAAC
YeeIE219D+ / GTTTTGCCGTACTTTCTGACTATTTCTTTAGCGCCCC
YeeIE219Q- / GGGGCGCTAAAGAAATATTGAGAAAGTACGGCAAAAC
YeeIE219Q+ / GTTTTGCCGTACTTTCTCAATATTTCTTTAGCGCCCC
YeeIF214M- / CAGAAAGTACGGCCATACATTCAGCAGGATCGCTGGC
YeeIF214M+ / GCCAGCGATCCTGCTGAATGTATGGCCGTACTTTCT
YeeIF222M- / GTTCTGGGGCGCTCATGAAATATTCAGAAAGTACGGC
YeeIF222M+ / GCCGTACTTTCTGAATATTTCATGAGCGCCCCAGAAC
YeeIFragment+ / GAACTGGCTGCAGATACAAGATTCTTTTGATGCTTC
YeeIfrg-Pst / AACGATGGCGTATCCCTGCAGGCCGCAGTTGTTGCC
YeeIP210S- / GGCAAAACATTCAGCACTATCGCTGGCTGCATAAGC
YeeIP210S+ / GCTTATGCAGCCAGCGATAGTGCTGAATGTTTTGCC
YeeIY205S- / CAGGATCGCTGGCTGCACTAGCATCAATGCTCGC
YeeIY205S+ / GCGAGCATTGATGCTAGTGCAGCCAGCGATCCTG
YeeIY243S- / CTGCAAAGGATCTTGTTGACTAAATTGGCAGAAACGTTGCCA
YeeIY243S+ / TGGCAACGTTTCTGCCAATTTAGTCAACAAGATCCTTTGCAG
Ymlcpms- / GGAGAAAATACCGCTCGAGGCGCCTTAG
Ymlcpms+ / GGACTGGAATGGCTGCAGGGTTTTCAT
References (supplementary informations)

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