Table S2.Hexamer, Adaptor, and PCR Primer Sequences
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Table S2.Hexamer, adaptor, and PCR primer sequences
Hexamer sequences (5' to 3')
TCCTTG / AATAGG / TGTAGG / CTAGGG / GTGGAA / GATAAGGGGTCA / ATAAGG / TGCTAT / CGTTGT / GTGACC / GATAAC
ACTGTT / AGAGGA / TCGAAC / CGTATA / GTAATG / GATAAA
AACGGT / GTACAC / AATGTG / CGGAAA / GGTGAA / GAGTAG
GAGCTC / AACGGA / AATCAG / CCTTAT / GGTAAA / GAGAGC
TGCGTT / AGCATC / AAGGTC / ATTGTG / GGGATA / GAGACG
TGGAGT / ACGTAC / ACGAGT / ATATCG / GGCCAA / GAGAAT
TCCGCA / AGAGTA / ACATTG / AGACGC / GGCACA / GAGAAA
TCTGCT / TCACCA / ACGTTT / ACATAC / GGATAT / GACTTT
AAGTGG / GCGACG / GAGGTA / AAGGGG / GGACAT / GACTAT
GTCCTT / GTTGTG / ATTGCA / AACGAG / GCACAT / GACCAA
TGGTAC / GCTGCA / ATTATC / AACGAA / GCAATC / GACATA
AGGTCA / GCTAGG / AATGAG / AACAGA / GATGGG / GACACA
GTGGGG / GTGTGC / GTAAGC / AACAAT / GAGTCG / GACAAT
TGGGGC / ATAATT / GGGAAA / TTGATA / GAGATG / GAATTC
GTCTGC / TGTATT / GCGTTT / TTATTA / GAGAGG / GAATGC
GCCGAT / CTCGAG / CGACCC / TGAGAC / GAATAG / GAATGA
TGTGCT / GGGTTA / CCTGTA / TCGAAG / CTCAAT / GAATCG
ACGCGT / ACTCAG / CACTAG / TATGTG / CTAGCA / GAATAC
GGGTCG / AGCAGT / AAGCAC / TATGGT / CTAGAG / GAATAA
TGGTGG / CGCTAT / TTGAGA / GTATGG / CGTTGG / GAACGA
AGTGGA / CTTAGT / TGCATA / GTATAA / CGTAAC / GAACAA
ACCGCA / AAGCGG / GTTAAC / CTTATA / CGCTAG / GAAATG
ACTGGA / GCTAGC / GCACTC / CGCACA / CGATAG / GAAAGA
CTGGTC / GAAGTG / GAGCAC / CAAAGG / CGATAA / GAAACT
GTTGCC / GACAGT / CCGACG / ATCGAT / CGAGAT / GAAACG
GACGCT / ATTCTA / GTTAGT / AACGAT / CCAGAT / GAAAAT
AAGGCT / ATCGTA / GTATAG / AACACG / CCAGAC / GAAAAC
TTGGGT / ATTTGC / AGTGGC / TGAGCA / CATGAC / CTTTTT
ACATTA / CAGATC / GGATTG / GTTTAG / ATTGAC / CTTAAT
GGCGTT / CGCATC / GGTAGT / GTTGTA / ATGACG / CTGCAA
GTGGCA / CTGTAC / AGCGAA / GGAAAA / AGAGGC / CTGAAA
ATTGCT / AAGGCG / TAGTGG / GCGATA / ACTGTA / CTCTTA
CTGGCT / GGCTCT / TTTCTG / GCAACG / ACGATA / CTCATA
TGCGGA / ATGTCC / TGTGCA / ATAACT / ACACTC / CTATTG
ACGCTG / CGTAGC / TCAATA / AATACA / ACAATT / CTATGC
CAGCTG / CCGCAT / GCAATA / TGTGGC / AATTGT / CTATAG
TGTTCG / AAGGTG / CTCATT / TGCGAG / AATTCT / CTATAA
TTGCCC / AGCGAG / CGAGTC / TGAATT / AATTAC / CTAGAT
GCGCGT / CCGAGA / CGAGCA / TGAAGG / AAGAAC / CTAGAC
TCGGAT / AAGCGA / GTGAAA / TCTCTT / AAATAC / CTAGAA
AGGTCT / TAGATA / GACTGT / TATAGA / TTTTTG / CTAATT
TGCACG / GGGTGA / GAAGCC / TAGGTA / TTGTTG / CTAATG
GAGTCC / CTGCAC / CGACTG / GTTAAT / TTAGGT / CTAATA
ATGAGA / CGGTAG / AAGTGA / GTTAAA / TGTCGA / CTAAAG
CTGTCA / CGAGCG / TAGTAG / GGATAA / TGTAAC / CGTAGA
GCGCGA / AGTCCT / CCACGA / GGACTC / TCGCGA / CGTAAA
AGTGTA / TCTTAG / GAAGAA / GCTAAG / TCAGAT / CGGATG
TGCTAC / GTGGAT / GTACGT / GAAGCG / TATCAT / CGCGAG
GGAGCG / GTGAAG / GGTCAC / GAACGG / GGTGAC / CGCAAG
GACTCA / GTGAAC / GCGGAA / CTGTGA / GGCGAC / CGCAAA
TAGTGC / AGGAAG / AGACGG / CTGTAA / GATACG / CGAGAC
CCCTTA / ACTGAC / GGACGG / CTATGT / CTGATG / CGAGAA
CTGTCG / ATGTAG / GTTGAA / CGATCT / CGGTGA / CGAACG
TGTCGC / AGTCCG / GATCTT / CGAATC / CGATTG / CGAAAT
ATCGGG / GTAGCG / CTTTGA / AATGGT / CCTGAT / CGAAAA
GTCTCT / TCTAGG / CTGTTC / AATGGG / CCCTAA / CCTGAC
CGTCTC / AATAGA / CGTGAT / AAGATG / AGATGA / CCTAGA
ATCTGG / TCTTCT / CAAATG / AACAAC / AAGTGT / CCCAAA
CCTGTC / GCGAGT / ATTTAA / AAACGG / AAACTA / CCATAA
TGCCGT / GAGGGG / ATCATG / TATTTG / AAACAA / CCAATT
GTGCGT / CATTGC / ATATAG / GCTGTA / TGAACG / CCAATG
GGCGGG / CATTCC / ATACCG / GCAACT / TATGTA / CATTGG
TGTTGC / TCGTGA / ATAACA / GACGAA / TAATCA / CATTAG
GCACTG / AGAGGG / AGACAT / CGGAGC / GATTGA / CATTAA
AGGCTG / TGAGTA / AAAATG / CCAAGT / AAAGAA / CATGTA
TGGGTC / GGGCGT / TTTTAG / CGAACC / TTAGTC / CATATT
GCTGTT / TTGTGG / CTCCAA / ATCTAT / AGAGAG / CATATG
GCTGGG / TCGGAC / ATACGA / GGAATG / TTTTTT / CATATC
ACGCAC / TCGAGC / AGCTAG / TTTACT / TTTTGT / CATATA
GTCGTG / ACAGAG / TTTGCG / TTGATG / TTTGTG / CATACT
CCTGGA / TGTTGT / TTATTT / TTCTTA / TTTGGC / CATAAT
GGGCGA / TGCACT / ACAGCT / TTATGA / TTTGAC / CATAAG
CGAGTG / GGGAGC / TTGACT / TTAATC / TTTCTA / CATAAC
ATCGGA / GAATTG / TGAAAG / TGTTTA / TTTATT / CAGTTT
CGTGTA / TCCTGA / TCTGCA / TGTAAT / TTTAGT / CAGACG
CGGCTC / GCATTG / TACACC / TGCAAT / TTGTAA / CAGAAA
TTGGCT / CGTGGC / GTGAAT / TGACTC / TTGCGA / CACATT
CGCTCA / GAGGGC / GGCAAA / TGAAGT / TTGACA / CACATA
AGTCGT / CGAAAG / GGACAG / TGAACA / TTGAAC / CACAAT
AGTTGT / TAGTAA / GAAGTC / TGAAAA / TTCATA / CAATGT
ATCGTG / CCACAG / GAAAGC / TCTAGA / TTATTG / CAATCG
TGACCA / GTCAAC / CGTTAA / TATGCC / TTATGT / CAATCA
ACGAGG / GGAGCT / CGCAAC / TATGCA / TTATGC / CAATAG
TTGGCC / TACTGA / CGATAC / TATCGT / TTATCA / CAATAC
ACCGAG / ACAATA / CCGCAA / TATCCA / TTATAA / CAACTG
TGGGAA / ATGACA / CCGAAT / TACTGT / TTAGGG / CAACGA
GACTGG / AGTAAG / CACAGT / TACAGC / TTAATT / CAACAG
GACCTC / CACCGA / AGTTGG / TAAGTA / TTAATG / CAACAC
CACTCT / AGATCA / AGAAAG / TAACTC / TTAACA / CAAATT
CTACAT / ATTTGG / AATCGG / TAACAC / TTAAAA / CAAATA
TTCTAC / CCCATA / AACGAC / GTCATT / TGTTAA / CAAAAT
TGTTGA / TGTAGT / TTGCTA / GTAGAA / TGTGAA / CAAAAA
GAGGCT / TGGACG / GTTTAT / GGTTTA / TGTCAT / ATTTGT
GGTGCG / ATCAGA / GTCTTG / GCGAAT / TGTAGA / ATTTAT
GGCGGA / ATAGGC / GGATCT / GATGTA / TGTAAG / ATTGGC
ATACTT / ATGAGT / GAGCAT / GATGCC / TGTAAA / ATTGGA
AGGTGT / ATACCA / GACAGG / GATGAT / TGCGAA / ATTATG
ATGCAC / ATAACG / GACACT / GATCTA / TGCAAC / ATTAAT
AGACGT / TTTAGA / CGCGAT / GAGTGT / TGATTG / ATGTTG
TCTGTG / TTCTGC / CCTTAC / GAGTAT / TGATAT / ATGTAA
AGTTCA / CTTTGC / AGACTT / GAGAGT / TGATAA / ATGGAA
GCGGAG / AGTGCG / AAGTAA / GACGAG / TGAGGG / ATGAAA
GGCTGT / AAGTTG / AAGAAG / GAATGT / TGAGAG / ATCTTA
ATAGGA / CTAGTA / TGTTCT / GAAGAG / TGACAG / ATCATA
ACTGGG / CGTACA / GTAGGT / CTTATG / TGAATG / ATATCC
AGAATC / AGATGG / GTAGAG / CTCTAA / TGAAAT / ATATAT
TGGGCT / CGAATA / GTATGT / CTAAGT / TCTTTG / ATAGAC
AGCGGA / TAGCAT / ATTTTT / CGTATC / TCTTGT / ATAATG
ATTGAG / AATTGA / TCGTGG / CGTAAT / TCTTAT / ATAAGC
CCTCAT / TAGTCC / GTGCGA / CGGAAT / TCTGTA / ATAAAA
TCGTTG / GTAGGG / GTGATT / CGAGGG / TCTATC / AGTTTA
TCTGGC / GGTGTC / GTAGAT / CCATAT / TCTACA / AGTAGA
CGGTGG / GAGCGG / GTAATA / CCAAGA / TCTAAC / AGCATA
CGTGCC / ACAGTT / GGTGCA / CCAAAT / TCGCAA / AGCACA
TGTCCG / AGCGTA / GCGAGA / CCAAAA / TCGAGA / AGATTG
TACGTT / GAAGGT / GCACAC / CATCAT / TCCAAA / AGATAT
ATTGCC / GTACCC / GAGGAC / CATCAC / TCATAT / AGACAC
ACTGTG / GGAGGC / CATGCG / CATAGT / TCATAG / ACTAGA
CTGCAG / CGTTTG / CAATGG / CATAGG / TCATAC / ACCAAG
TCACGT / GCATGA / AGATTA / CATAGA / TCATAA / ACCAAA
ATAGCC / CGTCGG / AAGTAT / CAGATG / TCAACA / ACATAT
TTCATG / AAAGGC / AAGAGT / CACAGA / TATTTT / ACATAA
TTGGTG / ATTAGG / TTACTT / CAATTG / TATTGG / ACAATG
ATGTTA / ACTATT / TCATGC / CAAGAC / TATTGC / ACAACA
AGTGGT / AGAGCA / TAGTGT / CAAGAA / TATTGA / ACAAAC
AGAGTC / AATCGC / TAGGGT / CAACAA / TATTAT / AATTTT
GAGGGA / AATCAT / GTTTAA / CAAACC / TATGAG / AATTTG
ACTCCA / ACATCA / GTACGA / ATTTTC / TATGAC / AATTTA
GCCTGA / AGAAGG / GCTATG / ATTATT / TATCGA / AATTGG
GCCCAA / TAGGGC / GACGCG / ATATTG / TATATT / AATTGC
ACCATG / GCCTAA / CTTGTC / ATATGG / TATATG / AATTCG
GGCTGA / AGGTAG / ATAACC / ATATAC / TATATA / AATTAT
GCTTCC / TTGATC / AACTAC / ATAATA / TATAAT / AATGTA
ACAGAT / TGGCTT / GTAGTG / ATAAGA / TATAAC / AATGGA
AACCTA / TCATTA / GGGACA / AGTTAT / TATAAA / AATCTT
TCCGTA / TAGCCA / GCACAA / AGTATA / TAGTTG / AATCTA
CTCACT / GGGTTT / AATCGT / AGATAA / TAGGAT / AATATG
CGTCTG / GCGCAT / AAACCG / AGAATT / TAGATT / AATATC
GTGCTA / GACTCT / GCGAAG / AGAATG / TAGAGG / AATAGT
ATGGTA / CCCAAT / GCAGGG / AGAAAC / TAGAGC / AATACT
GGTCGT / AACGCA / GCACTA / AGAAAA / TAGACG / AATAAT
TCGTTC / GTGTAT / ATCTAG / ACTCAA / TAGACC / AATAAG
GTCACG / GCGAGC / TAATAT / ACTATA / TAGACA / AAGCAA
GCTCAC / ATGGAC / GGTTGG / ACGTAG / TAGAAT / AAGAAA
GGTGTA / TCGCAT / CGGACA / ACGTAA / TAGAAA / AACTTC
GGGCAC / GTTGGG / CGCGAC / ACGGAA / TACTTG / AACTAT
GCCGAG / GCTGAT / CCATAG / ACGAAT / TACTAT / AACCAT
ATGTCT / CCTGAG / TGAGGA / ACGAAA / TACATT / AACATT
ACACGT / GAGTCA / GCTGAG / ACATTT / TACATA / AACATG
CAGCAG / CGACGC / GAGAAG / ACAAAT / TAATTT / AAATTT
TAGCGG / TACGAA / TTTTGA / AATGTT / TAATTG / AAATGC
GGTGAG / CTCGAA / TTTGAT / AATGTC / TAATTA / AAATGA
AGCAAT / GGTACA / TTATAT / AATCGA / TAATGT / AAATAT
ATTGGT / GGCAGC / TGGTGA / AATACG / TAATGG / AAATAA
ATTGTT / GGTAAT / TGACGC / AATAAC / TAATGC / AAACCT
ATGTCG / TAACGG / TATTGT / AAGAGG / TAATGA / AAACAT
TGCATT / ATTTTA / TATGCT / AAGAAT / TAATCT / AAAAGC
GCTCTG / ATGCAT / TATAGC / AAATGT / TAATCG / AAAACT
ATCTGT / ATATGC / TAGGTC / AAATCG / TAATAG / AAAAAT
GTTGTC / ATACTA / TAGCAA / AAAGTA / TAATAC / AAAAAG
GTCAGG / ATACGC / TACTAC / AAAGCA / TAAGTT / AAAAAA
ACATGT / ACTAAT / TACCAA / AAAGAG / TAAGAG / TTTGTT
CACTCG / TTCTAG / GTGCAT / AAACGA / TAAGAA / TTTAAG
AACGCG / TCTGAA / GTCGAA / AAAATT / TAACTG / TTGTCT
TGTGGG / GACGCA / GATTGC / AAAATA / TAACGA / TTGCAA
CGCTGT / CATTGA / CTACAA / AAAAGT / TAACAT / TGCTAA
ACAGGT / ATTAGA / CGCAAT / AAAACC / TAACAG / TCATTC
ACTTCC / AAATTG / CGAGTA / TTTGAA / TAAATT / TATCTG
TGTGAG / TGGAAC / CATTTA / TTGAAT / TAAAGC / TACAAC
TGTACT / CCCCAA / CAGAAT / TGTATG / TAAAAC / GTTGAC
CCTGCA / TGCGTA / CACAAA / TGTATC / GTTATT / GTCTTA
CGAGGC / ATGCTA / CAAGTG / TGTATA / GTTATG / GGTATA
GTTGGC / ATCACA / ATAGGT / TAAAAA / GTGTAA / GGTAGC
GCGAGG / GCGACA / ATAGCA / GTTCTG / GTCATA / GGACGC
ATTCTT / ATTGCG / AGAACA / GGCATA / GTATTG / GGACGA
ACACCT / AGAGAA / ACGAGA / GGAAAC / GTATGA / GCGTAG
CCCTGA / TACACG / AATGCT / GAAGGA / GTATCG / GCATAT
CGCGTG / GCGGAC / AACAGG / CTGTAG / GTATCC / GATCTG
TGTCTT / CTGTGG / AACAAA / CTGACA / GTATAT / GATAAT
GGGTAA / AATACC / AAAACG / CGGAAG / GTATAC / GAGGAA
ATGATA / TTGAAG / GAAATA / ATTCAA / GTAGAC / GAGATT
GGACCC / TGTGGA / CTACGT / ATGATT / GTAAGA / GACAGA
ATCTGC / AAGACA / CGACGA / ATAGTG / GTAAAC / GAACTA
ATATTT / ATATCA / CAGTTG / AGTATG / GTAAAA / GAAAAA
ACGTGG / AGAATA / ATGTAT / AGTAGC / GGTCAT / CTAACT
AACTGA / AGTCTA / AAAGCG / ACTTGT / GGTAGA / CTAACG
GTGTCC / ACAGGG / TCTACT / AATGCG / GGACAC / CGAGAG
AACCGC / GTACAT / TAAGGC / AATGAT / GGAAGT / CGAATG
TGACCG / GCCAAT / ATTGAA / AAGAGA / GGAAGA / CCTAAT
GCAAGA / GAGGGT / ATAGAG / AAAAAC / GCTATA / CCAAAG
GGGTAG / AACGTG / ATAAAG / GTTGGA / GCTAGA / CATAAA
CGTGCG / GTAACA / AGGATA / GTGTTG / GCGTAA / CAATAA
AGCACT / TGGAAT / ACGAAC / GGAGGG / GCGAAA / ATTCGA
ACTTTT / CGGTAA / AACACT / GCGCAA / GCATAG / ATCATT
AACTCG / CCGATG / AGGTTT / GAAATC / GCATAA / ATATGT
CGCAGA / AGTTAA / CGATGT / AGGATT / GCAGAA / ATAGTC
ACACTT / GGGAGG / TTAGTT / ACAGAC / GCACAG / ATAATC
CCGATA / GGACTG / TTGTAT / GTAGCT / GCAACA / ATAAAT
AACTCC / GCTCTT / TTAAGT / TTTTAC / GCAAAT / AGTGAC
GGGACG / CCCAAG / TGCACA / TTGTTC / GCAAAC / AGCAAA
TAGGCG / TCTAGT / TGATGG / TTGTGA / GCAAAA / AGAAGA
GCCACA / GCGTAT / TGATGC / TTAGTG / GATTTT / ACGCAA
TGTGCC / CTGCTA / TGAGAA / TGCTAG / GATTTG / AATGAC
TCCATT / CTGAGG / TCTCGA / TGATGT / GATTGT / AACACA
GGTCTC / CGTGAA / TCCAAG / TGAAGA / GATTGG / TGACTA
CAGTGG / TCAGAA / TATGTT / TCTTAC / GATTCT / GTCAGA
CGCACG / CCGCGA / TAGGGG / TCTTAA / GATTCG / GACATG
AATGCA / TATGTC / TACGTA / TCGTAA / GATTAT / CTGTAT
GGCGAG / GGCGTA / TACGAG / TCGAAA / GATGTC / CACGTA
CTACTG / GAATGG / TAAGCA / TCCTAA / GATGGA / ATCGAA
CGGAGG / GGTACC / GTAATC / TCATTG / GATGAA / ATAGAA
ATGTGC / ATTGGG / GGTATG / TATAGG / GATCGA / AAATTA
AAGCGT / ATGCGA / GCTTTG / TAGCAG / GATATT / TGCAAG
GTGAGT / AGAAAT / GAGACT / TAAGTG / GATATC / TAGAGA
ATACTG / TCGATG / GAAAGG / TAAGCG / GATATA / ATGCAA
GTTCAT / AGAACT / GAAAAG / TAAAGA / GATAGT / AGTGAA
GCAGTG / AGGACT / CTGAGA / GTTCGA / GATACT
Barcoded Forward Adaptors (5' to 3')
FwdAD-AGT
ACTAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTAGT
FwdAD-AAC
GTTAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTAAC
FwdAD-CGA
TCGAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTCGA
FwdAD-CTG
CAGAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTCTG
FwdAD-GTC
GACAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTGTC
FwdAD-GCG
CGCAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTGCG
FwdAD-TAA
TTAAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTTAA
FwdAD-TCT
AGAAGATCGGAAGAGCGTAATGATACGGCGACCACCGACACTCTTTCCCTACACGACGCTCTTCCGATCTTCT
Reverse Adaptor (5' to 3')
RevAD:AGATCGGAAGAGCTCCAAGCAGAAGACGGCATACGAGCTCTTCCGATCT
PCR Primers (5’ to 3’)
Forward primer: AATGATACGGCGACCACCGA
Reverse primer: CAAGCAGAAGACGGCATACG