Table S1water Temperatures and Salinities As Measured on Site

Table S1Water temperatures and salinities as measured on site

Site / Water temperature [°C] / Salinity [psu]
range / mean / median / range / mean / median
Duhnen / 0.1 - 22.1 / 10.5 / 9.5 / 7.7 - 30.1 / 21.0 / 22.6
Dorum / 4.0 - 25.6 / 13.1 / 13.5 / 0.9 - 27.7 / 15.8 / 18.3
Borkum / 2.0 - 22.0 / 11.6 / 11.0 / 7.1 - 33.3 / 27.4 / 28.7
Wremen / 4.0 - 22.8 / 12.1 / 10.8 / 0.7 - 22.5 / 14.3 / 16.4
Norderney / 0.0 - 21.1 / 10.1 / 9.0 / 11.9 - 32.8 / 26.2 / 28.7
Dyksterhusen / 0.0 - 21.0 / 13.4 / 13.9 / 5.1 - 21.6 / 14.9 / 14.8
Norddeich / 0.0 - 21.4 / 10.2 / 10.2 / 2.7 - 33.5 / 23.8 / 25.7
Burhave / 1.0 - 25.6 / 11.3 / 11.5 / 6.0 - 27.5 / 17.2 / 16.2
Bremerhaven / 1.0 - 24.8 / 12.3 / 13.5 / 1.6 - 19.2 / 11.6 / 11.8
Dedesdorf / 2.1 - 26.5 / 12.4 / 14.3 / 0.6 - 10.2 / 4.1 / 3.7

Table S2Pair-wise comparisons of Vibrio abundances by seasons according to Kruskal-Wallispost-hoc

tests as integrated in the R-package pgirmess following the kruskalmc function. Only pairs

significantly different at a level of p < 0.05 are depicted, all remaining pairs showed no significant difference.

V. alginolyticus / V. parahaemolyticus
obs,dif / critical,dif / difference / obs,dif / critical,dif / difference
Water / Jan-May / 109,56 / 77,13 / TRUE / Jan-Aug / 94,29 / 77,13 / TRUE
Jan-Jun / 87,22 / 76,28 / TRUE / Jan-Sep / 94,04 / 77,13 / TRUE
Jan-Jul / 112,01 / 77,13 / TRUE / Feb-Aug / 102,90 / 74,91 / TRUE
Jan-Aug / 132,21 / 77,13 / TRUE / Feb-Sep / 102,65 / 74,91 / TRUE
Jan-Sep / 118,31 / 77,13 / TRUE / Mar-Aug / 90,50 / 73,94 / TRUE
Jan-Oct / 101,21 / 77,13 / TRUE / Mar-Sep / 90,25 / 73,94 / TRUE
Feb-May / 91,77 / 74,91 / TRUE / Apr-Aug / 98,28 / 73,94 / TRUE
Feb-Jul / 94,22 / 74,91 / TRUE / Apr-Sep / 98,03 / 73,94 / TRUE
Feb-Aug / 114,42 / 74,91 / TRUE / May-Aug / 79,35 / 73,94 / TRUE
Feb-Sep / 100,52 / 74,91 / TRUE / May-Sep / 79,10 / 73,94 / TRUE
Feb-Oct / 83,42 / 74,91 / TRUE / Aug-Nov / 81,49 / 73,05 / TRUE
Mar-May / 82,25 / 73,94 / TRUE / Aug-Dec / 87,58 / 72,24 / TRUE
Mar-Jul / 84,70 / 73,94 / TRUE / Sep-Nov / 81,24 / 73,05 / TRUE
Mar-Aug / 104,90 / 73,94 / TRUE / Sep-Dec / 87,33 / 72,24 / TRUE
Mar-Sep / 91,00 / 73,94 / TRUE
Apr-May / 76,23 / 73,94 / TRUE
Apr-Jul / 78,68 / 73,94 / TRUE
Apr-Aug / 98,88 / 73,94 / TRUE
Apr-Sep / 84,98 / 73,94 / TRUE
Aug-Dec / 91,25 / 72,24 / TRUE
Sep-Dec / 77,35 / 72,24 / TRUE
Sediment / Jan-Jul / 95,44 / 76,28 / TRUE / Jan-Aug / 88,21 / 76,28 / TRUE
Jan-Aug / 84,44 / 76,28 / TRUE / Feb-Jul / 89,20 / 75,22 / TRUE
Jan-Sep / 86,36 / 76,28 / TRUE / Feb-Aug / 127,10 / 75,22 / TRUE
Feb-Jun / 95,23 / 74,34 / TRUE / Feb-Sep / 107,25 / 75,22 / TRUE
Feb-Jul / 131,46 / 75,22 / TRUE / Feb-Oct / 84,87 / 75,22 / TRUE
Feb-Aug / 120,46 / 75,22 / TRUE / Mar-Jul / 95,63 / 74,25 / TRUE
Feb-Sep / 122,38 / 75,22 / TRUE / Mar-Aug / 133,53 / 74,25 / TRUE
Feb-Oct / 94,16 / 75,22 / TRUE / Mar-Sep / 113,68 / 74,25 / TRUE
Feb-Nov / 82,99 / 74,34 / TRUE / Mar-Oct / 91,30 / 74,25 / TRUE
Mar-Jun / 79,07 / 73,36 / TRUE / Mar-Nov / 74,18 / 73,36 / TRUE
Mar-Jul / 115,30 / 74,25 / TRUE / Apr-Jul / 93,60 / 74,25 / TRUE
Mar-Aug / 104,30 / 74,25 / TRUE / Apr-Aug / 131,50 / 74,25 / TRUE
Mar-Sep / 106,23 / 74,25 / TRUE / Apr-Sep / 111,65 / 74,25 / TRUE
Mar-Oct / 78,00 / 74,25 / TRUE / Apr-Oct / 89,28 / 74,25 / TRUE
Apr-Jun / 82,97 / 73,36 / TRUE / May-Jul / 89,75 / 74,25 / TRUE
Apr-Jul / 119,20 / 74,25 / TRUE / May-Aug / 127,65 / 74,25 / TRUE
Apr-Aug / 108,20 / 74,25 / TRUE / May-Sep / 107,80 / 74,25 / TRUE
Apr-Sep / 110,13 / 74,25 / TRUE / May-Oct / 85,43 / 74,25 / TRUE
Apr-Oct / 81,90 / 74,25 / TRUE / Jun-Aug / 83,22 / 73,36 / TRUE
May-Jul / 91,28 / 74,25 / TRUE / Aug-Dec / 95,70 / 72,54 / TRUE
May-Aug / 80,28 / 74,25 / TRUE / Sep-Dec / 75,85 / 72,54 / TRUE
May-Sep / 82,20 / 74,25 / TRUE
Jul-Dec / 105,78 / 72,54 / TRUE
Aug-Dec / 94,78 / 72,54 / TRUE
Sep-Dec / 96,70 / 72,54 / TRUE

Table S3Overview over Vibrio abundances in relation to sampling sites

Water
V. alginolyticus [CFU100 mL] / V. parahaemolyticus [CFU/100 mL] / V. vulnificus [CFU/100 mL] / V. cholerae [CFU/100 mL]
range / mean / median / range / mean / median / range / mean / median / range / mean / median
Duhnen / 0 - 1900000 / 83759 / 400 / 0 - 150000 / 6322 / 0 / 0 - 0 / 0 / 0 / 0 - 100 / 4 / 0
Dorum / 0 - 4500 / 1189 / 400 / 0 - 460 / 60 / 0 / 0 - 110 / 5 / 0 / 0 - 100 / 5 / 0
Borkum / 0 - 6300 / 298 / 0.5 / 0 - 8900 / 387 / 0 / 0 - 0 / 0 / 0 / 0 - 0 / 0 / 0
Wremen / 0 - 18000 / 2279 / 470 / 0 - 500 / 79 / 0 / 0 - 300 / 18 / 0 / 0 - 110 / 5 / 0
Norderney / 0 - 15300 / 1384 / 12 / 0 - 7000 / 416 / 0 / 0 - 0 / 0 / 0 / 0 - 0 / 0 / 0
Dyksterhusen / 0 - 6000 / 970 / 350 / 0 - 6000 / 531 / 10 / 0 - 1000 / 46 / 0 / 0 - 140 / 7 / 0
Norddeich / 0 - 176000 / 9629 / 40 / 0 - 1000 / 62 / 0 / 0 - 0 / 0 / 0 / 0 - 0 / 0 / 0
Burhave / 0 - 1500 / 250 / 10 / 0 - 600 / 57 / 0 / 0 - 10 / 0.4 / 0 / 0 - 2 / 0 / 0
Bremerhaven / 0 - 520 / 101 / 30 / 0 - 400 / 36 / 0 / 0 - 690 / 45 / 0 / 0 - 0 / 0 / 0
Dedesdorf / 0 - 270 / 60 / 15 / 0 - 400 / 36 / 0 / 0 - 800 / 37 / 0 / 0 - 30 / 1 / 0
Sediment
V. alginolyticus [CFU/100 g] / V. parahaemolyticus [CFU/100 g] / V. vulnificus [CFU/100 g] / V. cholerae [CFU/100 g]
range / mean / median / range / mean / median / range / mean / median / range / mean / median
Duhnen / 0 - 1800000 / 110008 / 930 / 0 - 300000 / 13472 / 90 / 0 - 0 / 0 / 0 / 0 - 0 / 0 / 0
Dorum / 90 - >1100000 / 119317 / 15000 / 0 - 150000 / 15383 / 200 / 0 - 0 / 0 / 0 / 0 - 40 / 2 / 0
Borkum / 40 - 240000 / 16806 / 430 / 0 - 43000 / 2747 / 40 / 0 - 15000 / 556 / 0 / 0 - 0 / 0 / 0
Wremen / 40 - >1100000 / 64157 / 4100 / 0 - 150000 / 19269 / 140 / 0 - 930 / 43 / 0 / 0 - 0 / 0 / 0
Norderney / 0 - 5100000 / 288724 / 430 / 0 - 300000 / 13373 / 40 / 0 - 0 / 0 / 0 / 0 - 10 / 0 / 0
Dyksterhusen / 0 - 3600000 / 250988 / 13001 / 0 - 3100000 / 156350 / 1465 / 0 - 0 / 0 / 0 / 0 - 100 / 5 / 0
Norddeich / 40 - 160000 / 31962 / 4000 / 0 - 4000 / 624 / 174 / 0 - 0 / 0 / 0 / 0 - 70 / 3 / 0
Burhave / 0 - >1100000 / 81749 / 11000 / 0 - 210000 / 21763 / 2400 / 0 - 110 / 4 / 0 / 0 - 110 / 4 / 0
Bremerhaven / 0 - 93000 / 6938 / 430 / 0 - >110000 / 10618 / 40 / 0 - 110000 / 4794 / 0 / 0 - 0 / 0 / 0
Dedesdorf / 0 - 21000 / 1480 / 70 / 0 - 15000 / 761 / 90 / 0 - 24000 / 1564 / 0 / 0 - 60 / 2 / 0

Table S4Pair-wise comparisons of Vibrio abundances by sampling site according to Kruskal-Wallispost-hoc tests as integrated in the R-package pgirmess following the

kruskalmc function. Only pairs significantly different at a level of p < 0.05 are depicted, all remaining pairs showed no significant difference.

V. alginolyticus / V. parahaemolyticus
obs,dif / critical,dif / difference / obs,dif / critical,dif / difference
Water / Borkum-Duhnen / 64,38 / 63,51 / TRUE / Borkum-Burhave / 64,23 / 63,10 / TRUE
Borkum-Dyksterhusen / 78,85 / 65,02 / TRUE / Borkum-Dyksterhusen / 73,86 / 65,29 / TRUE
Borkum-Wremen / 79,74 / 65,02 / TRUE / Burhave-Norderney / 66,70 / 64,30 / TRUE
Sediment / Bremerhaven-Burhave / 68,46 / 64,30 / TRUE / Dedesdorf-Dyksterhusen / 69,33 / 66,45 / TRUE
Bremerhaven-Dorum / 71,29 / 67,29 / TRUE / Dyksterhusen-Norderney / 76,33 / 66,45 / TRUE
Burhave-Dedesdorf / 96,80 / 64,30 / TRUE
Dedesdorf-Dorum / 99,63 / 67,29 / TRUE
Dedesdorf-Norddeich / 80,98 / 64,96 / TRUE
Dedesdorf-Wremen / 69,44 / 66,45 / TRUE

Table S5Spearman´s Rank correlations between bacterial and environmental parameters (Part I). Data of the entire study are covered. Correlation

coefficients calculated on standardized data are shown in bold font in lower triangular part of the table. Significances of the correlations are shown in normal font in the upper right part of the table; *denotes significant coefficients after Bonferroni correction (p≤ 0.05/171 = 0.000292). Abbreviations: V.a.W=V. alginolyticus water, V.p.W. = V. parahaemolyticus water, V.v.W. = V. vulnificus water, V.c.W. = V. cholerae water, E.coliW = E. coli water, iEc W = intestinal enterococci water, V.a.S. = V. alginolyticus sediment, V.p.S. = V. parahaemolyticus sediment, V.v.S. = V. vulnificus sediment, V.c.S. = V. cholerae sediment, E.coliS = E. coli sediment, iEc S = intestinal enterococci sediment, space = stations (nominal variable), sal. = salinity, TOC = sediment TOC content, clay/silt = clay/silt content of sediment, W. temp. = water temperature, irrad. = global irradiance (sum of 3 days before sampling), sun = sunshine duration (sum of one week before sampling), clouds = cloud cover (mean of one week before sampling), rain = rainfall (mean of one week before sampling), wind dir. = wind direction (mean of one week before sampling), wind sp. = wind speed (mean of one week before sampling)

V.a.W / V.p.W / V.v.W / V.c.W / V.a.S / V.p.S / V.v.S / V.c.S / space / sal. / TOC / clay/silt
V.a.W / 0.000 / 0.093 / 0.678 / 0.000 / 0.000 / 0.214 / 0.690 / 0.019 / 0.073 / 0.138 / 0.020
V.p.W / *0,504 / 0.000 / 0.405 / 0.000 / 0.000 / 0.000 / 0.114 / 0.468 / 0.335 / 0.969 / 0.570
V.v.W / 0.109 / *0,312 / 0.302 / 0.045 / 0.002 / 0.000 / 0.555 / 0.019 / 0.161 / 0.815 / 0.088
V.c.W / 0.027 / 0.054 / 0.067 / 0.817 / 0.364 / 0.506 / 0.046 / 0.787 / 0.141 / 0.910 / 0.624
V.a.S / *0,506 / *0,423 / 0.130 / 0.015 / 0.000 / 0.045 / 0.086 / 0.006 / 0.001 / 0.000 / 0.000
V.p.S / *0,418 / *0,474 / 0.202 / 0.059 / *0,688 / 0.001 / 0.082 / 0.632 / 0.990 / 0.000 / 0.020
V.v.S / 0.081 / *0,246 / *0,570 / -0.043 / 0.129 / 0.215 / 0.539 / 0.001 / 0.218 / 0.112 / 0.003
V.c.S / 0.026 / 0.102 / -0.038 / 0.129 / 0.111 / 0.113 / -0.040 / 0.506 / 0.295 / 0.113 / 0.514
space / -0.151 / 0.047 / 0.152 / -0.018 / -0.177 / 0.031 / 0.218 / 0.043 / 0.000 / 0.919 / 0.183
sal. / 0.116 / -0.063 / -0.091 / -0.095 / 0.210 / -0.001 / -0.080 / -0.068 / *-0,436 / 0.295 / 0.000
TOC / 0.096 / -0.002 / 0.015 / -0.007 / *0,241 / *0,239 / -0.103 / 0.103 / -0.007 / -0.068 / 0.000
clay/silt / 0.150 / -0.037 / -0.110 / 0.032 / *0,300 / 0.150 / -0.193 / 0.042 / -0.086 / *0,308 / *0,499
W. temp. / *0,487 / *0,446 / *0,294 / 0.084 / *0,490 / *0,368 / *0,316 / 0.075 / 0.023 / 0.181 / -0.138 / 0.013
irrad. / *0,314 / *0,268 / 0.195 / 0.028 / *0,285 / 0.102 / 0.201 / 0.014 / -0.041 / 0.236 / -0.074 / 0.063
sun / *0,279 / 0.234 / 0.195 / 0.024 / 0.214 / 0.074 / 0.160 / -0.006 / -0.047 / 0.200 / -0.056 / 0.059
clouds / -0.136 / -0.151 / -0.154 / -0.005 / -0.230 / -0.107 / -0.159 / 0.002 / 0.059 / -0.164 / 0.058 / -0.019
rain / 0.077 / 0.001 / -0.012 / 0.045 / -0.030 / 0.001 / -0.115 / -0.080 / 0.006 / 0.050 / -0.018 / 0.035
wind dir. / 0.138 / 0.040 / -0.146 / -0.015 / 0.025 / -0.015 / -0.050 / -0.039 / -0.152 / 0.147 / -0.143 / -0.005
wind sp. / 0.022 / -0.192 / -0.186 / -0.086 / -0.174 / -0.154 / -0.131 / 0.001 / 0.131 / -0.134 / -0.022 / 0.092

Table S5Spearman´s Rank correlations between bacterial and environmental parameters (Part II). Data of the entire study are covered. Correlation coefficients calculated on standardized data are shown in bold font in lower triangular part of the table. Significances of the correlations are shown in normal font in the upper right part of the table; *denotes significant coefficients after Bonferroni correction (p≤ 0.05/171 = 0.000292). Abbreviations: V.a.W=V. alginolyticus water, V.p.W. = V. parahaemolyticus water, V.v.W. = V. vulnificus water, V.c.W. = V. cholerae water, E.coliW = E. coli water, iEc W = intestinal enterococci water, V.a.S. = V. alginolyticus sediment, V.p.S. = V. parahaemolyticus sediment, V.v.S. = V. vulnificus sediment, V.c.S. = V. cholerae sediment, E.coliS = E. coli sediment, iEc S = intestinal enterococci sediment, space = stations (nominal variable), sal. = salinity, TOC = sediment TOC content, clay/silt = clay/silt content of sediment, W. temp. = water temperature, irrad. = global irradiance (sum of 3 days before sampling), sun = sunshine duration (sum of one week before sampling), clouds = cloud cover (mean of one week before sampling), rain = rainfall (mean of one week before sampling), wind dir. = wind direction (mean of one week before sampling), wind sp. = wind speed (mean of one week before sampling)

W. temp. / irrad. / sun / clouds / rain / wind dir. / wind sp.
0.000 / 0.000 / 0.000 / 0.035 / 0.236 / 0.032 / 0.734
0.000 / 0.000 / 0.000 / 0.020 / 0.985 / 0.541 / 0.003
0.000 / 0.002 / 0.002 / 0.017 / 0.847 / 0.024 / 0.004
0.195 / 0.671 / 0.714 / 0.937 / 0.483 / 0.813 / 0.182
0.000 / 0.000 / 0.001 / 0.000 / 0.640 / 0.699 / 0.007
0.000 / 0.113 / 0.254 / 0.099 / 0.989 / 0.811 / 0.017
0.000 / 0.002 / 0.013 / 0.014 / 0.077 / 0.436 / 0.042
0.249 / 0.831 / 0.923 / 0.981 / 0.217 / 0.552 / 0.983
0.724 / 0.525 / 0.471 / 0.361 / 0.924 / 0.019 / 0.042
0.005 / 0.000 / 0.002 / 0.011 / 0.438 / 0.022 / 0.038
0.033 / 0.251 / 0.386 / 0.372 / 0.780 / 0.027 / 0.736
0.840 / 0.333 / 0.365 / 0.770 / 0.586 / 0.936 / 0.157
0.000 / 0.000 / 0.000 / 0.957 / 0.000 / 0.000
*0,718 / 0.000 / 0.000 / 0.001 / 0.001 / 0.000
*0,581 / *0,903 / 0.000 / 0.000 / 0.005 / 0.000
*-0,353 / *-0,576 / *-0,669 / 0.000 / 0.012 / 0.000
-0.003 / -0.214 / *-0,311 / *0,361 / 0.000 / 0.000
*0,263 / 0.211 / 0.182 / -0.162 / *0.371 / 0.000
*-0,259 / *-0,318 / *-0,305 / *0,249 / *0,367 / *0,266