Table S1a. Summary of Body Length Measurement in Dpy-5 Knockdown Experiments
Supplementary information
Table S1a. Summary of body length measurement in dpy-5 knockdown experiments
Genotype / Body length(μm) / Number of total worms / P value / Dpy percentage (%)*N2 / 1139.6±45.9 / 76 / －－－ / －－－
Control（Pcol-10::mCherry） / 985.4±72.8 / 88 / －－－ / －－－
U6::1~7 alldpy-5ts-gRNA；Pcol-10::mCherry / 989.6±95.8 / 118 / NS / 3.39 (4/118)
Pdpy-5::dCas9+emptyts-gRNA；Pcol-10::mCherry / 1053.1±108.9 / 108 / NS / 4.63 (5/108)
Pdpy-5::dCas9+1dpy-5ts-gRNA；Pcol-10::mCherry / 1056.9±69.5 / 112 / NS / 1.78 (2/112)
Pdpy-5::dCas9+2dpy-5ts-gRNA；Pcol-10::mCherry / 1017.5±102.9 / 89 / NS / 1.12 (1/89)
Pdpy-5::dCas9+3dpy-5ts-gRNA；Pcol-10::mCherry / 1077.9±78.9 / 92 / NS / 1.09 (1/92)
Pdpy-5::dCas9+4dpy-5ts-gRNA / 989.8±88.7 / 81 / NS / 2.46 (2/81)
Pdpy-5::dCas9+5dpy-5ts-gRNA；Pcol-10::mCherry / 985.3±85.8 / 113 / NS / 2.65 (3/113)
Pdpy-5::dCas9+6dpy-5ts-gRNA；Pcol-10::mCherry / 1023.8±82.4 / 93 / NS / 2.15 (2/93)
Pdpy-5::dCas9+7dpy-5ts-gRNA；Pcol-10::mCherry / 994.3±88.8 / 88 / NS / 1.14 (1/88)
Pdpy-5::dCas9+1~7alldpy-5ts-gRNA；Pcol-10::mCherry / 574.6±115.8 / 153 / p<0.05, compared to N2 , t’s test / 71.24 (109/153)
Pdpy-5::dCas9-KRAB；Pcol-10::mCherry / 1013.0±110.7 / 82 / NS / 6.10 (5/82)
Pdpy-5::dCas9-KRAB+1dpy-5ts-gRNA；Pcol-10::mCherry / 996.5±66.87 / 90 / NS / 2.22 (2/90)
Pdpy-5::dCas9-KRAB+2dpy-5ts-gRNA；Pcol-10::mCherry / 1008.7±70.4 / 78 / NS / 1.28 (1/78)
Pdpy-5::dCas9-KRAB+3dpy-5ts-gRNA；Pcol-10::mCherry / 1062.2±93.2 / 84 / NS / 1.19 (1/84)
Pdpy-5::dCas9-KRAB+4dpy-5ts-gRNA；Pcol-10::mCherry / 1010.8±59.8 / 72 / NS / 1.39 (1/72)
Pdpy-5::dCas9-KRAB+5dpy-5ts-gRNA；Pcol-10::mCherry / 912.6±85.3 / 87 / NS / 2.30 (2/87)
Pdpy-5::dCas9-KRAB+6dpy-5ts-gRNA；Pcol-10::mCherry / 989.7±66.8 / 82 / NS / 2.44 (2/82)
Pdpy-5::dCas9-KRAB+7dpy-5ts-gRNA；Pcol-10::mCherry / 1003.4±74.5 / 96 / NS / 2.08 (2/96)
Pdpy-5::dCas9-KRAB+1~7alldpy-5ts-gRNA；Pcol-10::mCherry / 487.9±97.6 / 162 / p<0.01, compared to N2 , t’s test / 85.19 (138/162)
*The percentage of animals with positive phenotypes (the body length is shorter than 800μm, Dpy phenotype), comparing with wild type N2 (body length~1140μm) and control transgenic animals. The number in brackets presents the number of animals with positive phenotypes in all observed transgenic worms.
Table S1b. Summary of body length measurement in dbl-1 activation experiments
Genotype / Body length(μm) / No. of total worms / P value / Lon percentage (%)*N2 / 1124.3±58.5 / 89 / －－－ / －－－
Control（Pcol-10::mCherry） / 1020.4±74.2 / 88 / －－－ / －－－
U6:: 1~6 alldbl-1ts-gRNA；Pcol-10::mCherry / 973.6±95.8 / 118 / NS / －－－
Pdbl-1::dCas9-VP160+emptyts-gRNA；Pcol-10::mCherry / 1032.8±86.6 / 82 / NS / 1.22 (1/82)
Pdbl-1::dCas9-VP160+1dbl-1ts-gRNA；Pcol-10::mCherry / 1045.9±81.5 / 76 / NS / －－－
Pdbl-1::dCas9-VP160+2dbl-1ts-gRNA；Pcol-10::mCherry / 969.6±85.3 / 87 / NS / －－－
Pdbl-1::dCas9-VP160+3dbl-1ts-gRNA；Pcol-10::mCherry / 958.7±98.2 / 72 / NS / －－－
Pdbl-1::dCas9-VP160+4dbl-1ts-gRNA；Pcol-10::mCherry / 921.4±76.5 / 71 / NS / 1.39 (1/72)
Pdbl-1::dCas9-VP160+5dbl-1ts-gRNA；Pcol-10::mCherry / 977.2±72.8 / 85 / NS / －－－
Pdbl-1::dCas9-VP160+6dbl-1ts-gRNA；Pcol-10::mCherry / 923.8±82.3 / 84 / NS / 2.38 (2/84)
Pdbl-1::dCas9-VP160+1~6dbl-1ts-gRNA；Pcol-10::mCherry / 1422.9±85.4 / 125 / P<0.05, Student’s test / 78.4 (98/125)
*The percentage of animals with positive phenotypes (the body length is longer than 1350μm, Lon phenotype), comparing with wild type N2 (body length~1125μm) and control transgenic animals. The number in brackets presents the number of animals with positive phenotypes in all observed transgenic worms.
Table S1c. Summary of body length measurement in experiments using worm conventional genetic tools
Genotype / Body length(μm) / Number of total worms / P value / Percentage of animals with positive phenotypes (%)N2 / 1128.9±57.2 / 96 / －－－ / －－－
empty vector RNAi / 1113.3±51.9 / 122 / －－－ / －－－
dpy-5RNAi / 338.8±76.8 / 146 / P<0.01 with empty vector RNAi, Student’s test / 95.2 (139/146) a
Pcol-10::mCherry / 1088.4±82.8 / 125 / －－－ / －－－
Pdbl-1::DBL-1+ Pcol-10::mCherry / 1528.6±105.8 / 132 / P<0.01 with Pcol-10::mCherry, Student’s test / 88.63 (117/132) b
a: The percentage of animals with positive phenotypes (the body length is shorter than 850μm), comparing with wild type N2 (body length~1130μm) and control transgenic animals. The number in brackets presents the number of animals with positive phenotypes in all observed transgenic worms.
b: The percentage of animals with positive phenotypes (the body length is longer than 1350μm), comparing with wild type N2 (body length~1130μm) and control transgenic animals. The number in brackets presents the number of animals with positive phenotypes in all observed transgenic worms.
Table S1d. C. elegansStrains used in this study
Strain name / Genotype / Method / ResourceN2 / --- / --- / CGC
MQD397 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)] / Microinjection and integration / Mengqiu Dong
PLD2018 / mfdEx2018[Pcol-10::mCherry] / Microinjection / This study
PLD2019 / mfdEx2019[U6::1~7 alldpy-5ts-gRNA；Pcol-10::mCherry] / Microinjection / This study
PLD2020 / mfdEx2020[Pdpy-5::dCas9+emptyts-gRNA; U6::dpy-5 ts-gRNA; Pcol-10::mCherry] / Microinjection / This study
PLD2021 / mfdEx2021[Pdpy-5::dCas9+1dpy-5ts-gRNA; Pcol-10::mCherry] / Microinjection / This study
PLD2022 / mfdEx2022[Pdpy-5::dCas9+2dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2023 / mfdEx2023[Pdpy-5::dCas9+3dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2024 / mfdEx2024[Pdpy-5::dCas9+4dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2025 / mfdEx2025[Pdpy-5::dCas9+5dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2026 / mfdEx2026[Pdpy-5::dCas9+6dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2027 / mfdEx2027[Pdpy-5::dCas9+7dpy-5ts-gRNA; Pcol-10::mCherry] / Microinjection / This study
PLD2028 / mfdEx2028[Pdpy-5::dCas9+1~7dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2029 / mfdEx2029[Pdpy-5::dCas9-KRAB;Pcol-10::mCherry] / Microinjection / This study
PLD2030 / mfdEx2030[Pdpy-5::dCas9-KRAB+1dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2031 / mfdEx2031[Pdpy-5::dCas9-KRAB+2dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2032 / mfdEx2032[Pdpy-5::dCas9-KRAB+3dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2033 / mfdEx2033[Pdpy-5::dCas9-KRAB+4dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2034 / mfdEx2034[Pdpy-5::dCas9-KRAB+5dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2035 / mfdEx2035[Pdpy-5::dCas9-KRAB+6dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2036 / mfdEx2036[Pdpy-5::dCas9-KRAB+7dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2037 / mfdEx2037[Pdpy-5::dCas9-KRAB+1~7 all dpy-5ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2038 / mfdEx2038[U6:: 1~6 alldbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2039 / mfdEx2039[Pdbl-1::dCas9-VP160+emptyts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2040 / mfdEx2040[Pdbl-1::dCas9-VP160+1dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2041 / mfdEx2041[Pdbl-1::dCas9-VP160+2dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2042 / mfdEx2042[Pdbl-1::dCas9-VP160+3dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2043 / mfdEx2043[Pdbl-1::dCas9-VP160+4dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2044 / mfdEx2044[Pdbl-1::dCas9-VP160+5dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2045 / mfdEx2045[Pdbl-1::dCas9-VP160+6dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2046 / mfdEx2046[Pdbl-1::dCas9-VP160+1~6 all dbl-1ts-gRNA;Pcol-10::mCherry] / Microinjection / This study
PLD2047 / mfdEx2047[Pdbl-1::DBL-1;Pcol-10::mCherry] / Microinjection / This study
PLD2048 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2048[Pges-1::dCas9;Pcol-10::mCherry] / Microinjection / This study
PLD2049 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2049[Pges-1::dCas9-KRAB;Pcol-10::mCherry] / Microinjection / This study
PLD2050 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2050[Pges-1::dCas9-VP160;Pcol-10::mCherry] / Microinjection / This study
PLD2051 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2050[Pges-1::dCas9;1~5 all ts-gRNAs;Pcol-10::mCherry] / Microinjection / This study
PLD2052 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2052[Pges-1::dCas9-VP160; 1~5 all ts-gRNAs;Pcol-10::mCherry] / Microinjection / This study
PLD2053 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2053[Pges-1::dCas9-VP160; 6~10 all ts-gRNAs;Pcol-10::mCherry] / Microinjection / This study
PLD2054 / hqIs92[pDYH59(Punc-119::unc-119+Ppud-2.2::GFP::pud-2.2)]; mfdEx2054[Phsp-16.2::dCas9-VP160; 6~10 all ts-gRNAsPcol-10::mCherry] / Microinjection / This study
PLD2055 / mfdEx2055[Pdpy-30::NLSSV40-GFP-NLSegl-13; Pdpy-30::NLSSV40-mCherry-NLSegl-13, Pges-1::dCas9-KRAB+ empty ts-gRNAs] / Microinjection / This study
PLD2056 / mfdEx2056[Pdpy-30::NLSSV40-GFP-NLSegl-13; Pdpy-30::NLSSV40-mCherry-NLSegl-13, Pges-1::dCas9-KRAB+ 1~5 gfpts-gRNAs] / Microinjection / This study
Table S1e. Summary of survival ratiosin zebrafish embryos injected with dCas9, dCas9-KRAB, dCAs9-VP16 or dCas9160 and multiple sgRNAs.
foxi1gRNAs / +dCas9-KRAB mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
1-5 / 465 / 481 / 96.67% / 595 / 615 / 96.75% / 799 / 823 / 97.08%
1-4 / 268 / 275 / 97.45% / 330 / 341 / 96.77% / 217 / 222 / 97.75%
2-5 / 212 / 218 / 97.25% / 211 / 216 / 97.69% / 623 / 641 / 97.19%
1-3 / 166 / 171 / 97.08% / 266 / 273 / 97.44% / 169 / 172 / 98.26%
3-5 / 271 / 280 / 96.79% / 154 / 159 / 96.86% / 187 / 192 / 97.40%
1,3,5 / 123 / 127 / 96.85% / 187 / 191 / 97.91% / 105 / 107 / 98.13%
1,2 / 131 / 137 / 95.62% / 167 / 172 / 97.09% / 157 / 163 / 96.32%
3,4 / 136 / 142 / 95.77% / 95 / 97 / 97.94% / 221 / 226 / 97.79%
4,5 / 127 / 129 / 98.45% / 115 / 117 / 98.29% / 311 / 315 / 98.73%
foxi1
gRNAs / +dCas9-VP160 mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
6-10 / 228 / 239 / 95.40% / 439 / 453 / 96.91% / 367 / 376 / 97.61%
6-9 / 151 / 154 / 98.05% / 122 / 124 / 98.39% / 414 / 423 / 97.87%
7-10 / 222 / 228 / 97.37% / 400 / 412 / 97.09% / 234 / 240 / 97.50%
6-8 / 165 / 172 / 95.93% / 92 / 96 / 95.83% / 181 / 186 / 97.31%
8-10 / 98 / 99 / 98.99% / 190 / 192 / 98.96% / 266 / 270 / 98.52%
6,8,10 / 122 / 125 / 97.60% / 165 / 169 / 97.63% / 203 / 206 / 98.54%
6,7 / 178 / 182 / 97.80% / 183 / 188 / 97.34% / 159 / 161 / 98.76%
8,9 / 166 / 170 / 97.65% / 106 / 108 / 98.15% / 114 / 116 / 98.28%
9,10 / 161 / 169 / 95.27% / 117 / 120 / 97.50% / 142 / 146 / 97.26%
foxi1
gRNAs / +dCas9-VP16 mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
6-10 / 176 / 182 / 96.70% / 113 / 117 / 96.58% / 389 / 396 / 98.23%
fgf8a
gRNAs / +dCas9-KRAB mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
1-5 / 338 / 352 / 96.02% / 410 / 429 / 95.57% / 396 / 406 / 97.54%
1-4 / 164 / 171 / 95.91% / 305 / 316 / 96.52% / 262 / 270 / 97.04%
2-5 / 184 / 194 / 94.85% / 271 / 284 / 95.42% / 206 / 214 / 96.26%
1-3 / 194 / 199 / 97.49% / 104 / 107 / 97.20% / 328 / 334 / 98.20%
3-5 / 78 / 81 / 96.30% / 169 / 174 / 97.13% / 94 / 97 / 96.91%
1,3,5 / 81 / 83 / 97.59% / 158 / 161 / 98.14% / 261 / 266 / 98.12%
1,2 / 143 / 150 / 95.33% / 139 / 147 / 94.56% / 89 / 93 / 95.70%
3,4 / 165 / 171 / 96.49% / 198 / 204 / 97.06% / 133 / 136 / 97.79%
4,5 / 67 / 71 / 94.37% / 142 / 148 / 95.95% / 327 / 338 / 96.75%
fgf8a
gRNAs / +dCas9-VP160 mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
6-10 / 169 / 179 / 94.41% / 503 / 526 / 95.63% / 668 / 694 / 96.25%
6-9 / 130 / 138 / 94.20% / 251 / 263 / 95.44% / 414 / 423 / 97.87%
7-10 / 146 / 152 / 96.05% / 307 / 320 / 95.94% / 449 / 466 / 96.35%
6-8 / 165 / 174 / 94.83% / 170 / 181 / 93.92% / 572 / 602 / 95.02%
8-10 / 232 / 240 / 96.67% / 143 / 147 / 97.28% / 139 / 147 / 94.56%
6,8,10 / 210 / 215 / 97.67% / 265 / 270 / 98.15% / 87 / 89 / 97.75%
6,7 / 188 / 191 / 98.43% / 158 / 160 / 98.75% / 154 / 156 / 98.72%
8,9 / 169 / 174 / 97.13% / 98 / 101 / 97.03% / 365 / 372 / 98.12%
9,10 / 187 / 194 / 96.39% / 160 / 165 / 96.97% / 282 / 289 / 97.58%
fgf8a
gRNAs / +dCas9-VP16 mRNA / +dCas9 mRNA / WTuninjected
survival / total / ratio / survival / total / ratio / survival / total / ratio
6-10 / 183 / 191 / 95.81% / 246 / 256 / 96.09% / 113 / 117 / 96.58%
Table S1f. Primers used in our study (5’→3’)
Primer name / Sequencedpy-30 promoter s / TTTATCTGCGATGGTGC
dpy-30 promoter As / TGAGATATCAAAGGCTTCTG
dbl-1 promoter s / AAACCACGGGCGAC
dbl-1 promoter As / ATATATGCTGTTGTAGCTGA
ges-1 promoter s / CAACATCACGGACCCAAT
ges-1 promoter As / CTGAAACTGTAAGACGCAGAC
dpy-5 promoter s / TTACTTATTTGTGCTTCGTC
dpy-5 promoter As / AAAGTTAGCAAGTTCAAAAG
hsp-16.2 promoter s / AGCGTTTCAAGTATCC
hsp-16.2 promoter As / GATTATAGTTTGAAGATTTC
act-1qRT-PCRs / CGTGAAATCGTCCGTGACAT
act-1qRT-PCRAs / TCCGTCAGGAAGTTCGTAGGA
dpy-5qRT-PCRs / ATGATGATGTGATGGGAGA
dpy-5qRT-PCRAs / TCCTGGTTGTCCGTCTT
dbl-1qRT-PCRs / ATGCAAGATCCTTTCAAGC
dbl-1qRT-PCRAs / TTGGCGGCACAATAAAG
gfpqRT-PCRs / ATGCAAGATCCTTTCAAGC
gfpqRT-PCRAs / TTGGCGGCACAATAAAG
dpy-5RNAis / TGTATGATGATGTGATGGGAG
dpy-5RNAiAs / GGCAGTATTCGGCATCG
dbl-1 overexpression s / GGCTCTCATGTGATGTAGGCT
dbl-1 overexpression As / CGGTGGGTATTCGAAGTAAC
fgf8aprobe s / AGTTATCTATTCCTTCACCTCTTTGCG
fgf8aprobe As / GACCCTTGGGCAACCTCTTCATGA
foxi1probe s / CTTCAGCAGCAGGACATCCTGGACATG
foxi1probe As / TGTTACCGATAAAGTTGCTAAAAC
fgf8aqRT-PCRs / CTTCACCTCTTTGCGTTTT
fgf8aqRT-PCRAs / CGGTCCGTCACCTTACTT
foxi1qRT-PCRs / CAGAACCAGGCCATCTCAACA
foxi1qRT-PCRAs / TTGACGCTGAAATGGTTGGA
pea3qRT-PCRs / CACAGCCTAAATGACCAC
pea3qRT-PCRAs / TTTCCTTGCGACCTCTAC
pax8qRT-PCRs / CCTGCGACATCTCCAGACA
pax8qRT-PCRAs / GCGATCTTCTCCACCACTTTT
Table S1g.ts-gRNAs used in our study
Gene or transgene name / ts-gRNA / Sequence information (5’→3’) / ts-gRNAspecificitydpy-5 / 1 / GGCAGCGCCGAATCCGACGA / Target NT strand*
2 / GGATGACAAGTGCAGCCCAA / Target NT strand
3 / GCTCGACGTTCGTCACGGAG / Target NT strand
4 / GTCCGCAAGAGCATTGGCTGT / Target NT strand
5 / GGTTTCCTGGAGCTCCGGCT / Target NT strand
6 / GTCCTGGGTTTCCTGGAGCTC / Target NT strand
7 / GCCAGTGCCTCCTGGCTCTCC / Target NT strand
dbl-1 / 1 / GATTCGTAGTAGAAATTAG / Target T strand**
2 / GAAAAAAAACTGTCTATTAG / Target T strand
3 / GGAAAATCTTTAAAAACATC / Target T strand
4 / GCCTGATTATGCGCTAAACAAG / Target T strand
5 / GCAGCCACTGGACATATCGTA / Target NT strand
6 / GACTATGAACGACTCTGTG / Target T strand
Ppud-2.2::gfp / 1 / GCACAAATTTTCTGTCAG / Target T strand
2 / GTGAAGGTGATGCAACATA / Target T strand
3 / GTGCTGAAGTCAAGTTTGA / Target NT strand
4 / GACCATCTAATTCAACAAGAAT / Target NT strand
5 / GAGTAGTGCAAATAAATTTAA / Target NT strand
6 / GTTCATGCTACATGAAACTAAC / Target NT strand
7 / GCTTTCTTTTGGTACACCTT / Target T strand
8 / GCAATACTGATATAAAACCTT / Target NT strand
9 / GAACTGCTTTTTAAAGTCAGT / Target T strand
10 / GCATTGTTCTGTCACACCTGA / Target NT strand
fgf8a / 1 / GATAACTCAACCGTGA / Target NT strand
2 / gagcatagtagcaaaacgcaaag / Target NT strand
3 / Ggattagtctacggctcacc / Target NT strand
4 / Ggttcggctgtaaagctggt / Target NT strand
5 / gaacgtcgccatcttcggcca / Target NT strand
6 / gaagcactcggagagtttag / Target NT strand
7 / GGTGAAGGAATATTTAAAAG / Target T strand
8 / GGTTCAGGGTGGGGGTTT / Target T strand
9 / GAAAAGGGGGCTTGTTCCTCC / Target T strand
10 / GGACCAACCCTACCATTTTAACC / Target T strand
foxi1 / 1 / GAAGTCAACGGTTACAGGTT / Target NT strand
2 / GGGTGGACACGTCTAAAAAA / Target NT strand
3 / GACTGTTTAAGCGCTAAAAA / Target NT strand
4 / GACGCCAGATTGATGCATAT / Target NT strand
5 / GAGAAGAAGTGTGACAGTGGG / Target NT strand
6 / GGAGGGTTATTTAACCTGTC / Target T strand
7 / GGTTTCTCAAGGAGAGTGTC / Target NT strand
8 / GGACTGGTCTGCTGGCTCGA / Target NT strand
9 / GGTGGTGATGGTGGTGATGA / Target T strand
10 / GGCTGTTCATCCACAGGTAA / Target T strand
* Target NT strand: ts-gRNA targets non-template DNA strand.
** Target T strand: ts-gRNA targets template DNA strand.