Supplementary materials

Table S1 Primers used to generate BM47 NLS mutants by site-directed mutagenesis.

Primers a / Nucleotide sequence (5′→3′) b
PR117A(+)
PK118A(+)
PR119A(+)
PR120A(+)
PR144A(+)
PK145A(+)
PR146A(+)
PK148A(+)
PR117A/K118A(+)
PR118A/K119A(+)
PR119A/K120A(+)
PR117A/K120A(+)
PR144A/K145A(+)
PR144A/R146A(+)
PR144A/K148A(+)
PK145A/R146A(+)
PR146A/K148A(+)
PR117A/K118A/R119A(+)
PK118A/R119A/R120A(+)
PR117A/R119A/R120A(+)
PR117A/K118A/R120A(+)
PK145A/R146A/K148A(+)
PR144A/R146A/K148A(+)
PR144A/K145A/K148A(+)
PR144A/K145A/R146A(+)
PR117A/K118A/R119A/R120A(+)
PR144A/K145A/R146A/K148A(+)
PK124A/R125A(+)
PK132A/K133A(+)
PK130A(+)
PK138A(+) / AAC ACG TTT AAT AGT GCT AAA CGG CGA GTC GTC
ACG TTT AAT AGT CGT GCA CGG CGA GTC GTC GCT
TTT AAT AGT CGT AAA GCG CGA GTC GTC GCT AAG
AAT AGT CGT AAA CGG GCA GTC GTC GCT AAG CGG
GTG GTG TCG ACG CCG GCT AAA CGA TTA AAA CAA
GTG TCG ACG CCG CGT GCA CGA TTA AAA CAA CAC
TCG ACG CCG CGT AAA GCA TTA AAA CAA CAC ACA
CCG CGT AAA CGA TTA GCA CAA CAC ACA AAC ACA
AAC ACG TTT AAT AGT GCT GCA CGG CGA GTC GTC GCT
ACG TTT AAT AGT CGT GCA GCG CGA GTC GTC GCT AAG
TTT AAT AGT CGT AAA GCG GCA GTC GTC GCT AAG CGG
AAC ACG TTT AAT AGT GCT AAA CGG GCA GTC GTC GCT AAG CGG
GTG GTG TCG ACG CCG GCT GCA CGA TTA AAA CAA CAC
GTG GTG TCG ACG CCG GCT AAA GCA TTA AAA CAA CAC ACA
GTG GTG TCG ACG CCG GCT AAA CGA TTA GCA CAA CAC ACA AAC ACA
GTG TCG ACG CCG CGT GCA GCA TTA AAA CAA CAC ACA
TCG ACG CCG CGT AAA GCA TTA GCA CAA CAC ACA AAC ACA
AAC ACG TTT AAT AGT GCT GCA GCG CGA GTC GTC GCT AAG
ACG TTT AAT AGT CGT GCA GCG GCA GTC GTC GCT AAG CGG
AAC ACG TTT AAT AGT GCT CGA GCG GCA GTC GTC GCT AAG CGG
AAC ACG TTT AAT AGT GCT GCA CGG GCA GTC GTC GCT AAG CGG
GTG TCG ACG CCG CGT GCA GCA TTA GCA CAA CAC ACA AAC ACA
GTG GTG TCG ACG CCG GCT AAA GCA TTA GCA CAA CAC ACA AAC ACA
GTG GTG TCG ACG CCG GCT GCA CGA TTA GCA CAA CAC ACA AAC ACA
GTG GTG TCG ACG CCG GCT GCA GCA TTA AAA CAA CAC ACA
AAC ACG TTT AAT AGT GCT GCA GCG GCA GTC GTC GCT AAG CGG
GTG GTG TCG ACG CCG GCT GCA GCA TTA GCA CAA CAC ACA AAC ACA
CGG CGA GTC GTC GCT GCG GCG GCG TTT AAT TCG AAA
TTT AAT TCG AAA TCC GCA GCA ATT CCT ATC GGC AAA G
CGG GCG TTT AAT TCG GCA TCC AAA AAA ATT CCT
AAA ATT CCT ATC GGC GCA GTG GTG TCG ACG CCG

a Two complementary primers were used for each desired mutation. The shown primer was plus-sense sequence.

b The desired mutations were shown in boldface letters.

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