Supplementary materials

Table S1. Mapping of reads to reference transcripts

File name / Sample / Total reads* / Mapped reads** / Unmapped reads*** / Mapping rate / Total mapped reads
TN1307R3450_1.fq / Keumkang / 29,411,995 / 25,267,589 / 4,144,406 / 86% / 263,506,589
TN1307R3450_2.fq / 26,860,854 / 23,179,457 / 3,681,397 / 86% / 243,863,952
TN1307R3454_1.fq / KC59 / 37,602,435 / 29,948,530 / 7,653,905 / 80% / 179,753,282
TN1307R3454_2.fq / 36,517,125 / 29,150,622 / 7,366,503 / 80% / 176,560,170

*Total reads: numbers of reads mapped to reference gene sequences after pre-treatment.

**Mapped reads: numbers of reads mapped to more than one reference gene sequences.

***Unmapped reads: number of reads not mapped to reference gene sequences.

Table S2. BLAST annotation of transcript de novo assembly

BLAST alignment* / BLAST after filtering** / Arabidopsis annotations***
No. of transcripts / 97,481 / 53,337 / 50,380 (10,040 unique in TAIR)

*BLAST alignment: unique protein after comparison with Phytozome database and BLASTP.

**Filtering: e-value ≤ 1e-5 and best match.

***Number of wheat proteins with an Arabidopsis gene ID.

Table S3. Up- and down-regulated genes according to RNA sequencing analysis

Target / Regulation pattern / No. of DEGs / No. of
annotated DEGs
Keumkang vs. KC59 / Up-regulated / 11,910 / 10,768
Down-regulated / 1,544 / 693

DEG, differentially expressed gene.

Table S4. Categorization of 521 differentially expressed genes based on their molecular function according to Gene Ontology (GO) DAVID analysis (http://david.abcc.ncifcrf.gov/)

GO term / Up-regulation (number of genes) / Down-regulation (number of genes) / Total
Response to stress / 58 / 12 / 70
Response to external stimulus / 10 / 0 / 10
Response to endogenous stimulus / 19 / 0 / 19
Regulation of metabolic process / 29 / 0 / 29
Response to chemical stimulus / 35 / 10 / 45
Regulation of molecular function / 4 / 0 / 4
External encapsulating structure / 11 / 0 / 11
Apoplast / 5 / 0 / 5
Transcription factor activity / 24 / 0 / 24
Ion binding / 43 / 0 / 43
Unknown protein / 235 / 26 / 261
Total / 473 / 48 / 521

Table S5. List of differentially expressed genes in Iksan370 identified by RNA sequencing (Attached as an Excel file)

Table S6. List of primer sequences used for quantitative real-time PCR

Name / Sequence (5’–3’)
Arf 2aF / TGAGGTATCGCACGGGGAG
Arf 2aR / GGTTGGGTTGGGTTGTCGT
Arf 2bF / TGATGCTGGTGGGAGACGAT
Arf 2bR / TTGATTCGGGAAGATGGGC
Tor 1F / CACAGCGGCAACCAAGAAG
Tor 1R / CCGCAGCAAACAACTCGC
Tor 2F / CACACTCTCTTTCTGCTTCGTCT
Tor-2R / CTCCTAACACCAACATCAGCATC
Tor 3F / CTCATGCAACCTCCCCGA
Tor-3R / AAAAGTCACGCCCTGTTAGC
Arl-1F / ACATGGAGGGCTGCGGAAG
Arl-1R / AAAGAGGTAACGGCGAAGGAG
Exp-B2F / TGCGCGAGTCATGGGGATC
Exp-B2R / CGATGGAGCGGTAGAAGGTG
Cesa-1F / ACCGCACGCCAACCATCG
Cesa-1R / CCACACTGCCCCATAGCGAC
Cesa-3F / AGGGTCTTATGGGCAGGCAA
Cesa-3R / GGGCCAGCGAGACGGGTA
Cesa-4F / ATCCCGCCCACCACGCTC
Cesa-4R / TCCGGTTCTGCCTCCCCA
Cesa-6F / CAGAACAGGACCCCGACGAT
Cesa-6R / CCAGACCGCACTCCTCCAGA
Cesa-7F / GCATCTCCGACGCCATCAAC
Cesa-7R / GTCCTGTTCTGCCTCCCCAT
Cesa-8F / GCTGATCCCGCCCACGAC
Cesa-8R / GCGGCCCATGAGACCCTT
actin2 F / GAATCCATGAGACCACCTAC
actin2 R / AATCCAGACACTGTACTTCC
Ta2291-F / GCTCTCCAACAACATTGCCAA
Ta2291-R / GCTTCTGCCTGTCACATACGC

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