Table 2. Gene Clones Used in This Work
Gene 1 / Clonename / Origin / Nature / Insert size
(kb) / Source
abd-A
/ 3111 / D. melanogaster / genomic / 8.0 / S. Celniker(California Institute of Technology)
Abd-B / L8082 / D. melanogaster / genomic / 11.3 / E. Sánchez Herrero
(Centro de Biología Molecular Severo Ochoa, Madrid)
Atp / 47.1 / D. melanogaster / cDNA / 3.5 / D. M. Fambrough
(Johns Hopkins University¸ Baltimore)
btn / btn / D. melanogaster / cDNA / 1.7 / P.A. Beachy
(Johns Hopkins University, Baltimore)
Cha / pCha-2 / D. melanogaster / cDNA / 2.4 / P.M. Salvatierra
(Beckman Research Institute of the City of Hope, California)
Dhfr / Dhfr / D. melanogaster / cDNA / 0.5 / V.K. Walker
(Queen´s University, Canada)
dsx / pGPE114 / D. buzzatii / genomic / 0.4 3 / This work
Supplement Table 2. (continued)
Gene 1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
-Est 3 / F5B5 / D. buzzatii / genomic / 6.0 / J.S.F Barker
(University of new England, Australia)
-Est 10 / DbE10 / D. buzzatii / genomic / 1.3 / J.S.F Barker
(University of new England, Australia)
Fdh / tsr-1/EMBL4 2 / D. melanogaster / genomic / 2.6 / R. González
(Universitat de Barcelona)
ftz / h6 / D. hydei / genomic / ~15.0 / D. Maier
(Universität Hohhenheim)
fzo / 1.1 / D. melanogaster / cDNA / 2.4 / K.G. Hales
(Stanford University School of Medicine)
Gcn2 / DGCN2 / D. melanogaster / cDNA / 5.6 / C. de Haro
(Centro de Biología Molecular Severo Ochoa, Madrid)
Gst / VG8 / D. melanogaster / genomic / 8.0 / S. Bernstein
(San Diego State University)
Supplement Table 2. (continued)
Gene 1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
H / Dh10/3 / D. hydei / genomic / ~15.0 / D. Maier
(Universität Hohhenheim)
hh / hh / D. melanogaster / cDNA / 1.6 / P.A. Beachy
(Johns Hopkins University, Baltimore)
ImpE3 / IMPE3 / D. melanogaster / genomic / 4.5 / J.W. Fristrom
(University of California, Berkeley)
janA janB / pBB2 / D. melanogaster / genomic / 1.0 / J.-A. Lepesant
(Institut Jacques Monod, Université Paris 7)
lab / c241 / D. melanogaster / cDNA / 2.1 / R. de Frutos
(Universitat de València)
mod / 1.5 R1 / D. melanogaster / cDNA / 1.5 / J. Pradel
(Université d`Aix-Marseille II)
neur / cDNA-3.9 2 / D. melanogaster / cDNA / 2.8 / A. Laughon
(University of Wisconsin)
Supplement Table 2. (continued)
Gene 1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
Nmdmc / 6-1 / D. melanogaster / cDNA / 1.0 / A. Laughon
(University of Wisconsin)
opa / a1 / D. melanogaster / cDNA / 1.9 / S. Sakonju
(University of Utah)
OS-E / B370 / D. melanogaster / genomic / 0.7 / J.R. Carlson
(Yale University)
Pak / DPAK / D. melanogaster / cDNA / 3.2 / L. Lim
(National University of Singapore)
pb / 5a1 / D. melanogaster / cDNA / 1.1 / T.C. Kaufman
(Indiana University)
Pbprp3 / B347 / D. melanogaster / cDNA / 0.9 / J.R. Carlson
(Yale University)
Pi3K92E / pSL30 / D. melanogaster / genomic / 3.5 / M.D. Waterfield
(University College of London)
Supplement Table 2. (continued)
Gene 1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
Pkc98E / dPKC98F / D. melanogaster / cDNA / 2.7 / C. Zuker
(University of California, San Diego)
pll / S597 / D. melanogaster / cDNA / 1.9 / S.A. Wasserman
(University of Texas Southwestern Medical Center)
Ppp / 5-8 / D. melanogaster / cDNA / 3.9 / D.E. Siekhaus
(Stanford University)
put / PUT / D. melanogaster / cDNA / 2.5 / H. Jäckle
(Max-Planck-Institut für Biophysikalische Chemie, Göttingen)
Pxd / DmPO22b / D. melanogaster / genomic / 4.3 / G. Petersen
(Universität Heidelberg)
ro / pvirSal / D. virilis / genomic / 9.0 / U. Heberlein
(San Francisco General Hospital)
Rb97D / Rb97D / D. melanogaster / genomic / 8.0 / S.R. Haynes
(National Institutes of Health, Bethesda)
Supplement Table 2. (continued)
Gene1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
RpL1 / Dm2pL1 / D. melanogaster / cDNA / 1.2 / F. Graziani
(Instituto Internazionale di Genetica e Biofisica, Napoli)
stg / pcSTG1.8 / D. melanogaster / cDNA / 2.3 / P.H. O´Farrell
(University of California, San Francisco)
Syn / syn-1 / D. melanogaster / cDNA / 3.4 / E. Buchner
E. Buchner (Lehrstuhl für Genetik, Würzburg)
T48 / T48 / D. melanogaster / cDNA / 2.5 / D. Strutt
(University of Sheffield)
TfIIF / dTFIIF30 / D. melanogaster / cDNA / 1.1 / J.T. Kadonaga
(University of California, San Diego)
Tl / pTL / D. melanogaster / cDNA / 5.3 / C. Hashimoto
(Yale University)
Tm1 / 2 / D. melanogaster / genomic / 6.7 / E.A. Fyrberg
(Johns Hopkins University, Baltimore)
Supplement Table 2. (continued)
Gene1 / Clone
name / Origin / Nature / Insert size
(kb) / Source
Tm2 / 1 / D. melanogaster / genomic / 1.6 / E.A. Fyrberg
(Johns Hopkins University, Baltimore)
trx / 6C12 / D. melanogaster / cDNA / 2.2 / I.B. Dawid
(National Institute of Child Health and Human Development, Bethesda)
wdn / SS3 / D. melanogaster / genomic / 6.1 / J.-A. Lepesant
(Institut Jacques Monod, Université Paris 7)
zen / pDmzen 2 / D. melanogaster / cDNA / / R. de Frutos
(Universitat de València)
1 See Ranz et al. (1997) and Ranz et al. (1999) for information on the previously used gene clones, and FlyBase (1999) for full name and/or additional information on each gene.
2 The available clone derives from the clone mentioned.
3 Primers to amplify dsx in D. buzzatii (K.C. Burtis, pers. comm.):5´-GGTCTAGACTATTGCCAAAAGCT-3´ (nucleotides 2324-2343 in D. melanogaster dsx sequence, with three extra bases at 5´); 5´-TTCTGCAGCTCACCCCCGTCATAGA-3´ (nucleotides 2512-2493 in D. melanogaster dsx sequence, with four extra bases at 5’). PCR product 339 bp; annealing temperature 48º for 1 min.