Ta: Annealing Temperature; P: Polymorphic; M: Monomorphic

Supplementary Table S1. Characteristics of other 94 polymorphic polynucleotide microsatellite loci from silver carp genome.

Locus / Accession
no. / Primer sequence (5' - 3') / Sequence
repeat motif / Approximate
size (bp) / Ta
(℃) / Polymorphisms
silver carp / bighead carp
Hysd7631-1 / KC189157 / F-TCTTACGGCTGTAGAACGAC / (ATT)8 / 322 / 54 / p / m
R-ATAGATTAGGGGTGTAACGGT
Hysd145-1 / KC189158 / F-ACTACTTGTAATACCGCCC / (AAAAT)6 / 266 / 50 / p / m
R-GACATTTACTTCATTGTGGAC
Hysd666-2 / KC189159 / F-TTCTGACAGGAACGCCGATAG / (TTTCT)21 / 300 / 56 / p / m
R-CCCTCAAGTTGTTCCAAACCTG
Hysd17-1 / KC189160 / F-GCACTAGCAATGCCAAGG / (TAAATG)4 / 156 / 56 / p / p
R-CATAGAAACACCAAATACCAGG
Hysd19-1 / KC189161 / F-CTTCCCTTCCATTGAAACTCT / (AGAT)10 / 122 / 50 / p / p
R-ACTCACTGATAAGAGGGGCTAC
Hysd460-1 / KC189162 / F-TAACCCTCTGGTGCTCTTC / (ATT)11 / 125 / 52 / p / m
R-TGGAGTAATGATGCTGAAAAT
Hysd27-2 / KC189163 / F-CTATTTGAGAATTTAGGGTG / (TGT)9 / 202 / 59 / p / m
R-TTTGTGAAGACAGCGAGA
Hysd45-2 / KC189164 / F-GGAGAGTAGCCTGTGTGAAG / (ACT)10 / 317 / 52 / p / m
R-TTATTACCGTAGAATGAGCC
Hysd26-1 / KC189165 / F-TCTAATGTCCTGATTTGAAGC / (TAG)8 / 251 / 59 / p / p
R-CAAACCCTAATCCTAATGCT
Hysd9-1 / KC189166 / F-GAGGTATTCCCAGAAACAAGTG / (ATATT)9 / 208 / 60 / p / P
R-GGATTCCCTGAGCCTACATT
Hysd209-1 / KC189167 / F-GTCATCCCTTTTCTCTCACAC / (ATT)9 / 282 / 54 / p / P
R-CTCTAATATGGCAACTGGAATG
Hysd10-2 / KC189168 / F-TCAAGGGTGCCTAAAGTTC / (TAA)8 / 138 / 59 / p / m
R-TTTTCTCCCTAAGAGGTTGT
Hysd119-1 / KC189169 / F-TGAGCAGAGGACTTGGTTAGG / (AAT)10 / 293 / 50 / p / m
R-GTCTTTCATACTGACACTCTGGC
Hysd233-1 / KC189170 / F-GCCGACACGACAAAGACAT / (ACT)11 / 261 / 61 / P / p
R-GCCTAAGCGGACAAACACTC
Hysd86-1 / KC189171 / F-TAGGAAATGGTCTGATTGCTG / (TAT)12 / 256 / 61 / P / P
R-CAACACACTGATAATCTGCTGAG
Hysd264-2 / KC189172 / F-ACCTATTGTATCCGTTGGC / (TTC)10 / 269 / 56 / p / m
R-CTGCCTCATTAAGCTGTG
Hysd221-1 / KC189173 / F-ATGTGCCTTAGTGTTGAATGC / (TAGA)10 / 263 / 63 / P / P
R-GTAAAGCGACCTTGAGTTGG
Hysd63-1 / KC189174 / F-CCACTGGCAAGGAAACT / (ATT)11 / 271 / 63 / p / m
R-GACAGCAAGAATGTAAGGC
Hysd156-1 / KC189175 / F-TCTGTGGTAAGTTGGATAG / (ATA)10 / 227 / 52 / p / m
R-CTTTGTTACAGTCCATCAC
Hysd698-2 / KC189176 / F-GTATGGCACATTTGGCTGA / (TAT)11 / 257 / 63 / p / P
R-CCAATAAGAGACACTCGCAC
Hysd749-2 / KC189177 / F-GAACACACTTCTTCATTTAG / (AAATA)11 / 274 / 58 / p / M
R-TGTTCCTATTCCCATCAG
Hysd644-2 / KC189178 / F-TTTAAGTTGATATTGCTGCTA / (ATA)10 / 202 / 50 / p / P
R-ATTATGCTGATTTGCTGTTC
Hysd407-1 / KC189179 / F-CAAAGCAGTAAAGGCAAAAC / (TCT)10 / 275 / 59 / p / M
R-TACTTGACTTCCTTTGTTTG
Hysd355-2 / KC189180 / F-GATCACTGGCTGTCAATTATG / (AGAT)11 / 281 / 56 / p / P
R-TTGGAATGTGGCTAAAGT
Hysd1237-1 / KC189181 / F-GATCAGATGCGTGTCACTA / (TAC)10 / 249 / 56 / p / M
R-GAACGAAGGTCTTATGGGTG
Hysd1006-2 / KC189182 / F-CGACCCACTTACAATATCAG / (ATT)10 / 242 / 48 / p / P
R-GGACTTACGAAAAGACCAA
Hysd837-2 / KC189183 / F-CGTCCGCCTTCGTTTGTT / (TTC)10 / 253 / 56 / p / P
R-CAGGAGGATGAGGAAGAGC
Hysd571-2 / KC189184 / F-CTGCTCTGAGGCATGTCGA / (ATT)18 / 253 / 53 / p / M
R-GTTTGAGCATGTTTAGGTCC
Hysd16-2 / KC189185 / F-CCTTGGACTCTTTAGCCAGACT / (ATAAT)7 / 367 / 63 / p / M
R-GCTTCAAAGGGTTCAAATCG
Hysd6-2 / KC189186 / F-CAGGAGTGTAAAGCAAGGAG / (CAAT)10 / 61 / p / M
R-GGTCTGGTAAAATATGGAATC
Hysd617-1 / KC189187 / F-GATACAGAACATTTCATTGC / (TTGA)13 / 228 / 63 / p / P
R-CTTCTGAGGAGTGGCTTTC
Hysd692-1 / KC189188 / F-CTTATGCTTGTTCTTTTGTG / (TTGA)20 / 254 / 59 / p / M
R-GTTCCTTTGTATTGTTATGG
Hysd513-2 / KC189189 / F-ATGGCACTCCACAGCACTT / (ATAG)12 / 284 / 59 / p / M
R-CATTCAAATATGGTAAAACTC
Hysd173-1 / KC189190 / F-ATCCTTTTCCTTCGCATCAT / (AAT)11 / 160 / 59 / p / M
R-TTGTTGGTGTTGTTGTTTGG
Hysd225-1 / KC189191 / F-AAGCCAATGTCCCTACAATAAC / (AGT)13 / 281 / 57 / p / P
R-TAAAGAAACACCACGCACCT
Hysd1005-1 / KC189192 / F-GCTCTATTCAGGATGGGATG / (AATC)17 / 294 / 61 / p / M
R-AGCAGAACCAGAAACTTCAAC
Hysd1005-2 / KC189193 / F-CTCTACGATGGTGTCACGGTT / (TAC)11 / 246 / 61 / p / M
R-CAAGGAGTGTGGCAAGTCAGT
Hysd28-1 / KC189194 / F-AGAGACCAATCATCACCGT / (TTA)9 / 262 / 57 / p / P
R-TGTACTCCTGCTGCCATAG
Hysd51-1 / KC189195 / F-ATTTCGTCATCAGAATAGTGC / (ATTT)16 / 320 / 57 / p / P
R-AGATACTTTCTAACCTCTTGGC
Hysd47-1 / KC189196 / F-AGCCAACATCAACAAGCCAC / (ATT)11 / 239 / 61 / p / P
R-CATAGATAACCGTCACAGCCG
Hysd493-2 / KC189197 / F-CGCAATCTCCAAGTGACG / (ATA)14 / 257 / 48 / p / M
R-TTGCCTGCGTAACTGACC
Hysd94-2 / JX499358 / F-ATGTTCAGCACCCAAGTCCC / (GATA)12 / 257 / 60 / p / M
R-GGCAAATCTATGAGCGGTCTG
Hysd456-2 / KC189198 / F-GGTGGCAATAATGATAAAAA / (TAT)11 / 224 / 57 / p / P
R-AAGGAGCAATGTTATGGTA
Hysd344-1 / KC189199 / F-CCAGGAAGAAACACCAAC / (ATT)9 / 288 / 48 / p / M
R-CTGAATGTTTTCTCAAGTAG
Hysd399-1 / KC189200 / F-CGCTGAGCACTGTATGTAT / (TAT)49 / 300 / 60 / p / M
R-AGTATTCTCGTCGCTTTATA
Hysd381-2 / KC189201 / F-CAAAAGAGCCATTCATTCC / (ATA)24 / 232 / 56 / p / m
R-CAGAATGAATGACTGTGAATC
Hysd110-2 / KC189202 / F-AGCCATCCTGGTGTTCGTCT / (TAC)15 / 359 / 59 / p / M
R-GCCTGGTTCTGTCACATTCG
Hysd110-3 / KC189203 / F-TCCCCGAATGGACAACCTAAC / (TTTC)19 / 134 / 48 / p / M
R-TTCATTGGGCTCGGTTTATTG
Hysd149-2 / KC189204 / F-GCTTTGTTTCCTGTAACTGCTG / (AGTTCA)7 / 195p / 59 / p / M
R-AAATGACCACTGAAGGCGAG
Hysd365-2 / KC189205 / F-CTGTCCTCTAACTGTGTGCC / (GAA)37 / 286 / 48 / p / M
R-AGTTTCGTCCAAGGCTGCG
Hysd886-1 / KC189206 / F-ACAAAGCAGGTATTGGTAGC / (AGAA)8 / 146 / 48 / p / p
R-ATGTCTAAGCACTTGACCGT
Hysd180-1 / KC189207 / F-ACACATCCCAGAATGACTCGC / (AGAC)7 / 294 / 54 / p / m
R-GCCTGTCAGATTTACATAACTCCC
Hysd301-1 / KC189208 / F-GGATAGTTCACACAAAAGATGGC / (TTTAT)7 / 151 / 57 / p / M
R-ACCCTTGGAGAGCACTGTTTC
Hysd301-2 / KC189209 / F-GAGAACTGTATCCTTGTTGTGC / (TTA)41 / 353 / 60 / p / M
R-GGTTGAGCAGATTAAGTTTGAC
Hysd360-1 / KC189210 / F-CCCTTAGAATGACTTGAAGATGT / (AGAT)8 / 289 / 60 / p / P
R-GAGGTTTGTTTGGATTTGCT
Hysd360-2 / KC189211 / F-ACAGCAGCCAAAGGAATGAG / (AAT)10 / 129 / 48 / p / M
R-TCTTGAAAAGCACCAGCCAC
Hysd511-1 / KC189212 / F-TCATCATTCGGTGCCCAGTTCTC / (CAAGT)5 / 297 / 60 / p / p
R-TTCCTTGCCGCTGTCGCCTT
Hysd182-1 / KC189213 / F-GCGTATCAGTAGATGGATAGTG / (ATT)12 / 230 / 48 / p / m
R-ATAGGTAAAGGAGCAAGAGC
Hysd82-1 / KC189214 / F-CAAGTTTGGCAACCTGTCTG / (TCTA)17 / 311 / 57 / p / p
R-TCACAATACCACAAACCAACAG
Hysd348-2 / KC189215 / F-TACTGGTGCTTAGAAATGGC / (TAA)25 / 282 / 48 / p / M
R-GTATTTTGTATAGGCACATCC
Hysd318-2 / KC189216 / F-GGTTCAGGTTACCAATCAC / (AAT)16 / 192 / 48 / p / M
R-CAATCACGGTCAAAGCATA
Hysd302-1 / KC189217 / F-AACTGTCAACTCAAACTTCT / (TTA)10 / 283 / 57 / p / M
R-CACAAACCTCTACACAAAC
Hysd302-2 / KC189218 / F-GGCACAAGAGTCACATAAAT / (GAGT)25 / 220 / 57 / p / M
R-CACTGTAAATAACTAGAAAGC
Hysd539-1 / KC189219 / F-GATTGGATTCCTGTGACTG / (ATAAT)12 / 230 / 61 / p / M
R-GCTGAGAGCAGAGAAAGAG
Hysd423-1 / KC189220 / F-GCAAACCAAGCAAAACGC / (AAT)10 / 242 / 59 / P / M
R-TGACGCTTCTGTTGACTTTG
Hysd349-2 / KC189221 / F-CCGTCTATTCCCATAAAGC / (ATCC)16 / 264 / 48 / p / p
R-GAGATACAAGGTTTTCGGC
Hysd126-1 / KC189222 / F-CACAAGTTCTGGTTTGGGTC / (AAT)11 / 223 / 61 / p / M
R-ATTTGGTAGGATTAAGGGATG
Hysd188-1 / KC189223 / F-CAGAGCAGTCAGTTGTTAGGG / (ATT)10 / 269 / 61 / p / P
R-AAATCCGTAATAGGGGCAC
Hysd291-3 / KC189224 / F-ACAACACGGCAGGAAACGCT / (AGG)10 / 166 / 48 / p / M
R-TGTCTGCTGTCCTTTGGCTGG
Hysd291-2 / KC189225 / F-CTGAGTGAGTCCCGTCCTTC / (ATT)11 / 257 / 61 / p / P
R-GTCCAGATGGCTATCCGAGT
Hysd272-1 / KC189226 / F-TCTGGGAAAACAAAAGGGTG / (ATCT)12 / 298 / 61 / p / P
R-TCACTACCAACAACTTTATGCG
Hysd1328-1 / KC189227 / F-CAGCACGCATCCCAGGTTAG / (TAT)12 / 272 / 54 / p / P
R-TAAAGACAGTTGACGCTTGGCT
Hysd1328-2 / KC189228 / F-AATCGGGCTAAGGTAAGAAG / (GGAT)7 / 259 / 56 / p / P
R-ACTGAAGACTGGAGTAATGGAG
Hysd786-1 / KC189229 / F-GAAAATGTGGTCAGGCGATG / (TTAT)7 / 262 / 54 / p / M
R-AGGTTGACTGCTGTTGGACTTC
Hysd393-1 / KC189230 / F-TAATGAGGGCTGTCAAAGTG / (GTTA)8 / 134 / 56 / p / P
R-TTGAACAAAATCTGCTTCCT
Hysd324-2 / KC189231 / F-GAGAAGCATCTGAACGCCAC / (TTTC)9 / 260 / 54 / p / M
R-CACGAGCACTGTCAGGAGAA
Hysd412-2 / KC189232 / F-CATCGTGTTGCTCATTCGC / (ATCT)9 / 227 / 48 / p / M
R-CGTTACCTGGCATTGAGC
Hysd440-2 / KC189233 / F-GCACAGTTCTTTTAGGATGG / (GATA)7 / 299 / 56 / p / P
R-GAGAGTGCCTCAAACAAAC
Hysd34-1 / KC189234 / F-AGGTCATCCCTTCTTCATAAC / (TCTA)10 / 190 / 54 / p / M
R-GATAAAAGGAGGAAAAACCC
Hysd451-1 / KC189235 / F-TGAACGAAACCGCAACCTGTC / (AGAT)22 / 211 / 54 / p / M
R-GGCTATGCACAACATGCCAGTC
Hysd408-1 / KC189236 / F-AGGTTCTACTGATTGGGGTT / (AAAG)15 / 237 / 52 / p / m
R-GGACAACTACCAAACACCTCT
Hysd922-1 / KC189237 / F-CTGTAGAATCACAACGAAACC / (ATTT)9 / 230 / 52 / p / P
R-ACCAATCTCAAAATCTGCTG
Hysd640-1 / KC189238 / F-TCCACCTGCTGTTTCCACTG / (ATCT)9 / 264 / 52 / p / M
R-ACGAAACTCTTCAGGCTCACAG
Hysd371-1 / KC189239 / F-TTCAGTTCTAATGCGGTCCTT / (TAA)9 / 217 / 48 / p / P
R-CTCTGGTGGTGTTCGGTCAT
Hysd8-1 / KC189240 / F-AGAAATGAAGAAAACTCGGG / (TTA)11 / 248 / 54 / p / M
R-CACCTTTGAACGGTAGTGTAT
Hysd358-2 / KC189241 / F-TGTATCACCCTGACCTTTTGC / (TATC)8 / 141 / 56 / p / M
R-CTGTGGAGTCAAGCCGATTAG
Hysd39-1 / KC189242 / F-ATGCGAGTTTACCAGGATGAC / (TATAA)11 / 215 / 52 / p / M
R-GACAAAAAGGGTTGGGACAC
Hysd626-1 / KC189243 / F-AAAATGCCTGTGATTCTGTC / (AACAT)6 / 285 / 52 / p / M
R-ATGTCCACCTACTCACCCTC
Hysd626-2 / KC189244 / F-ATGCCAGCCTGTTATTTTAC / (TTA)9 / 216 / 56 / p / M
R-ATGCCACCCAGTGTTGATTA
Hysd696-1 / KC189245 / F-ACGCTTTCTCTAAGTGGTTGTG / (CATT)7 / 261 / 52 / p / M
R-GCTCTATCTTCATTCTGCCAAC
Hysd294-1 / KC189246 / F-TACATACAGGTAGTCACGCAAT
R-ATCCCTTAGCTCATATCACATC / (ATA)9 / 101 / 58 / p / m
Hysd294-2 / KC189247 / F-GTATCCCCGTTTCCTTATTTCT / (TTCT)7 / 286 / 56 / p / P
R-TTCACAGGCGTTTTACCCAT
Hysd7-2 / KC189248 / F-AAGAGGTTTATGTGGGATTAGC / (GTA)10 / 306 / 48 / p / P
R-CTGATTTTCATTGGAGCGTG
Hysd19-2 / KC189249 / F-CTTTTGTGCTTGGGAGTGG / (TAA)9 / 293 / 48 / p / M
R-AATGTGACGAGACAAGAAACG
Total / 94 / 35

Ta: annealing temperature; P: polymorphic; M: monomorphic.