Supplementary Material 4 – Summary of detected QTLs for aerial morphogenetic traits revealed in M. truncatula recombinant inbreed lines of LR1, LR4, LR5 and LR6 populations
Chromosome Number / Population/traita / Season/Year / QTL Position and confidence interval in each map (cM) / QTL Position and confidence interval in the consensus map (cM) / LOD / R2(%) / Additive effectb
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1 / LR1/FT
LR4/FT
LR6/FT
LR1/BER
LR4/BER
LR4/BER
LR5/BER
LR6/BER
LR5/LMS
LR6/LMS
LR6/LMS
LR1/NPB
LR1/NPB
LR4/NPB
LR5/NPB
LR6/NPB
LR1/LPB
LR4/LPB
LR5/LPB
LR5/LPB
LR1/DPB
LR6/DPB
LR5/NI
LR1/ADM
LR1/ADM
LR5/ADM / Autumn/2005
Spring/2002
Spring/2008
Autumn/2005
Spring/2002
Spring/2003
Spring/2005
Spring/2008
Spring/2005
Spring/2007
Spring/2008
Spring/2005
Autumn/2005
Spring/2002
Autumn/2005
Spring/2008
Autum/2005
Autum/2004
Spring/2005
Autumn/2005
Spring/2005
Spring/2008
Spring/2005
Spring/2005
Autumn/2005
Spring/2005 / 82.5 (78-89)
51.7 (38-56)
51.8 (43-58)
80.5 (70-89)
39.0 (26-49)
45.0 (34-63)
39.5 (35-42)
35.6 (28-34)
71.0 (68-78)
57.5 (52-66)
55.5 (50-58)
72.8 (53-80)
78.5 (74-81)
51.1 (43-55)
4.0 (0-20)
63.5 (60-68)
78.5 (76-84)
51.7 (52-58)
38.3 (32-41)
78.6 (73.-82)
82.5 (78-89)
2.0 (0-8)
0.01 (0-3)
80.5 (71-87)
82.5 (78-89)
55.5 (50-73) / 69.7 (66-75)
51.7 (38-56)
54.8 (39-63)
68.2 (62-75)
39.0 (26-49)
45.0 (34-63)
27.0 (23-30)
27.0 (17-25)
62.8 (60-66)
62.7 (55-71)
60.1 (52-63)
63.8 (56-67)
66.7 (64-68)
51.7 (43-55)
1.7 (0-12)
68.8 (65-73)
66.8 (65-71)
51.7 (52-58)
26.0 (20-29)
67.1 (64-68)
69.7 (66-75)
1.1 (0-4)
0.004 (0-1.3)
68.2 (63-73)
69.7 (66-75)
51.5 (43-64) / 4.1
3.0
2.8
4.4
4.0
3.4
7.1
3.2
4.4
5.1
13.0
3.3
13.0
5.7
2.5
3.1
10.7
3.2
6.2
7.2
6.1
3.6
3.2
3.0
12.5
3.0 / 5.45
7.54
5.94
7.80
20.90
14.81
19.48
6.86
8.59
10.28
26.22
8.23
28.33
15.82
8.18
6.95
19.01
7.23
20.52
21.8
10.95
8.91
8.46
6.33
20.62
9.43 / -29.329
27.139
26.508
0.005
0.006
0.005
0.005
0.0003
-5.106
-5.840
-8.389
0.290
0.527
0.218
-0.245
-0.436
6.618
-0.149
4.542
-0.787
0.087
-0.054
-0.542
0.741
1.162
-0.793
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2 / LR6/FT
LR6/FT
LR6/FT
LR4/BER
LR4/BER
LR6/BER
LR4/LMS
LR5/LMS
LR6/LMS
LR1/NPB
LR6/NPB
LR4/LPB
LR5/LPB
LR5/LPB
LR6/LPB
LR6/ADM / Spring/2007
Spring/2007
Spring/2008
Spring/2002
Spring/2003
Spring/2008
Autumn/2004
Spring/2005
Spring/2007
Spring/2005
Spring/2007
Spring/2002
Spring/2005
Autumn/2005
Spring/2007
Spring/2007 / 2.0 (0-19)
38.4 (33-42)
32.4 (27-39)
66.4 (62-68)
64.4 (55-70)
71.6 (62-78)
60.4 (55-70)
60.0 (51-70)
71.6 (66-80)
61.9 (53-70)
11.9 (4-22)
64.4 (62-69)
60.0 (51-68)
46.2 (45-58)
2.0 (0-12)
69.6 (66-79) / 0.56 (0-17.549)
41.2 (31.3-44.03.9)
30.3 (25.3-42.3)
66.4 (62-68)
64.4 (55-70)
63.0 (56-67)
60.4 (55-70)
60.4 (51-65)
63.0 (59-68)
58.6 (51-65)
9.7 (1-20)
64.4 (62-69)
60.4 (51-64)
47.3 (46-58)
0.56 (0-9)
61.7 (59-68) / 2.8
2.5
3.1
8.5
4.0
2.7
2.7
3.8
3.1
2.9
3.2
5.3
4.2
4.1
2.6
3.5 / 5.14
4.56
6.02
33.02
13.21
7.39
3.27
5.22
8.74
5.78
11.66
18.11
9.70
11.28
16.76
10.47 / -31.743
29.831
26.791
0.008
0.005
0.0003
2.400
3.947
-5.416
-0.239
-0.408
4.086
3.681
4.906
2.479
-1.466
3
3
3
3
3
3
3
3
3
3
3
3
3 / LR1/LMS
LR1/LMS
LR1/LPB
LR6/LPB
LR4/LPB
LR4/LPB
LR4/LPB
LR5/LPB
LR4/LPB
LR6/DPB
LR4/NI
LR1/ADM
LR1/ADM
LR4/ADM / Spring/2005
Autumn/2005
Autumn/2005
Spring/2008
Spring/2002
Spring/2003
Autumn/2004
Spring/2005
Autumn/2004
Spring/2007
Spring/2002
Spring/2005
Autumn/2005
Spring/2003 / 59.0 (54-60)
4.0 (0-14)
12.9 (9-17)
67.8 (62-72)
54.6 (50-58)
52.6 (50-57)
51.6 (48-58)
0.01 (0-14)
51.6 (48-58)
71.8 (67-76)
58.6 (54-60)
19.7 (11-31)
12.9 (0-14)
56.6 (51-60) / 59.4 (56-60)
1.6 (0-24)
20.6 (4-26)
55.9 (51-59)
54.6 (50-58)
52.6 (50-57)
51.6 (48-58)
0.01 (0-17)
51.6 (48-58)
59.1 (55-62)
58.6 (54-60)
28.2 (12-36)
20.6 (0-24)
56.6 (51-60) / 2.5
2.8
3.1
3.3
3.3
3.9
3.3
2.9
3.3
3.1
2.6
2.7
5.2
3.4 / 3.83
5.89
4.91
11.03
11.31
8.68
7.31
7.01
7.31
8.05
8.59
7.33
7.82
11.48 / -2.287
4.353
1.191
3.185
3.942
4.203
4.146
0.925
4.146
5.791
0.297
0.800
0.714
0.142
4
4
4
4
4
4
4
4
4
4
4
4 / LR1/FT
LR1/FT
LR1/BER
LR1/LMS
LR1/LPB
LR1/LPB
LR4/LPB
LR4/DPB
LR4/NI
LR4/NI
LR4/ADM
LR4/ADM / Spring/2005
Autumn/2005
Autumn/2005
Autumn/2005
Spring/2005
Autumn/2005
Spring/2002
Spring/2002
Spring/2002
Spring/2003
Spring/2002
Spring/2003 / 65.9 (62-69)
65.9 (61-70)
65.9 (64-68)
67.9 (66-70)
56.8 (53-68)
65.9 (63-68)
72.6 (68-78)
66.6 (63-78)
70.6 (51-72)
0.01 (0-6)
70.6 (66-78)
0.01 (0-12) / 72.7 (68-76)
72.7 (67-77)
72.7 (70-75)
75.2 (72-77)
63.4 (59-75)
72.7 (69-75)
72.6 (68-78)
66.6 (63-78)
70.6 (51-72)
0.01 (0-6)
70.6 (66-78)
0.01 (0-12) / 3.3
2.4
4.2
4.1
2.9
4.1
4.0
4.1
5.7
4.3
4.2
2.6 / 3.52
2.87
6.71
7.58
5.66
6.27
11.27
13.26
20.77
15.25
14.84
6.67 / 26.380
21.550
-0.005
-4.906
-0.495
-2.217
-3.170
-0.064
-0.379
-0.436
-0.105
-0.088
5
5
5
5
5
5
5
5
5
5
5
5 / LR4/FT
LR4/BER
LR4/BER
LR5/BER
LR5/NPB
LR4/LPB
LR4/DPB
LR4/DPB
LR6/DPB
LR4/ADM
LR4/ADM
LR5/ADM / Spring/2003
Spring/2002
Spring/2003
Autumn/2005
Spring/2005
Spring/2003
Spring/2002
Spring/2003
Spring/2008
Spring/2002
Spring/2003
Spring/2005 / 0.01 (0-5)
0.01 (0-8)
0.01 (0-9)
22.7 (16-27)
62.3 (51-69)
2.0 (0-10)
2.0 (0-9)
0.01 (0-5)
4.01 (0-18)
0.01 (0-9)
0.01 (0-7)
54.3 (49-68) / 0.01 (0-5)
0.01 (0-8)
0.01 (0-9)
15.1 (10-19)
61.3 (50-69)
2.0 (0-10)
2.0 (0-9)
0.01 (0-5)
3.2 (0-14)
0.01 (0-9)
0.01 (0-7)
53.6 (48.68) / 2.9
3.4
2.7
2.5
2.7
2.8
4.6
12.3
2.6
3.4
7.2
2.7 / 2.23
11.27
7.30
6.53
7.06
6.84
21.79
32.24
6.94
10.19
21.76
10.90 / 24.260
-0.004
-0.003
-0.005
0.334
-3.039
-0.079
-0.116
-0.045
-0.088
-0.162
0.657
6
6
6
6
6
6
6 / LR6/FT
LR6/FT
LR6/FT
LR6/NPB
LR4/LPB
LR4/NI
LR5/ADM / Spring/2007
Spring/2007
Spring/2008
Spring/2007
Autumn/2004
Spring/2002
Spring/2005 / 10.0 (7-11)
16.2 (12-24)
14.9 (11-18)
35.7 (35-49)
31.1 (22-48)
49.9 (44-61)
0.01 (0-7) / 15.4 (10.7-16.8)
21.1 (17.6-28.2)
20.0 (16.8-22.7)
39.0 (38-46)
31.1 (22-48)
49.9 (44-61)
0.01 (0-7) / 9.0
9.6
7.3
2.7
2.6
2.7
3.0 / 19.18
19.43
15.10
6.38
5.95
7.61
9.20 / 61.460
61.376
42.748
0.296
1.364
0.225
0.781
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7
7 / LR1/FT
LR1/FT
LR4/FT
LR4/FT
LR4/FT
LR4/FT
LR5/FT
LR5/FT
LR5/FT
LR4/BER
LR4/BER
LR1/LMS
LR1/LMS
LR4/LMS
LR5/LMS
LR6/LMS
LR1/NPB
LR4/NPB
LR4/NPB
LR4/NPB
LR1/LPB
LR4/LPB
LR4/LPB
LR4/LPB
LR5/LPB
LR5/LPB
LR5/LPB
LR1/DPB
LR4/DPB
LR5/DPB
LR6/NI / Spring/2005
Autumn/2005
Spring/2002
Spring/2003
Autumn/2004
Autumn/2004
Spring/2005
Spring/2005
Autumn/2005
Spring/2003
Autumn/2004
Spring/2005
Autumn/2005
Autumn/2004
Spring/2005
Spring/2008
Spring/2005
Spring/2002
Spring/2003
Autumn/2004
Spring/2005
Spring/2002
Spring/2003
Autumn/2004
Spring/2005
Spring/2005
Autumn/2005
Spring/2005
Autumn/2004
Autumn/2005
Spring/2008 / 60.1 (53-68)
59.7 (57-61)
53.5 (52-68)
59.4 (56-65)
53.5 (52-55)
61.4 (58-64)
51.0 (48-57)
63.2 (58-64)
67.3 (58-76)
59.4 (56-67)
61.4 (55-67)
55.7 (52-62)
30.0 (19-38)
59.4 (58-62)
53.0 (50-67)
23.8 (8-35)
40.0 (30.48)
63.4 (61-66)
64.9 (52-68)
57.4 (56-60)
64.1 (62-66)
55.5 (52-62)
59.4 (58-64)
59.4 (56-63)
61.2 (49-74)
35.8 (25-48)
72.5 (70-78)
40.0 (32-54)
51.3 (44-56)
37.8 (28-37)
58.1 (55-60) / 57.4 (48-66)
56.8 (53-58)
53.5 (52-68)
59.4 (56-65)
53.5 (52-55)
61.4 (58-64)
44.9 (41-51)
57.3 (52-58)
61.5 (52-71)
59.4 (56-67)
61.4 (55-67)
51.2 (46-59)
25.8 (16-32)
59.4 (58-62)
47.0 (43-61)
22.8 (8-31)
35.6 (26-43)
63.4 (61-66)
64.9 (52-68)
57.4 (56-60)
61.8 (59-63)
55.5 (52-62)
59.4 (58-64)
59.4 (56-63)
55.3 (42-69)
29.1 (19-41)
67.5 (64-72)
35.6 (28-49)
51.3 (44-56)
31.0 (21-30)
51.2 (48-52) / 28.0
23.5
7.4
23.0
14.1
12.8
5.6
5.3
4.8
5.3
3.8
9.6
4.5
18.9
6.9
3.5
5.0
8.5
3.2
10.7
8.0
5.8
11.0
18.2
3.2
4.0
3.8
4.5
7.3
2.7
5.1 / 39.21
34.96
24.08
62.78
31.06
31.11
12.30
10.27
15.79
16.51
9.70
19.0
13.82
30.23
12.20
10.35
14.78
26.22
11.06
22.84
14.39
17.62
32.38
38.01
8.18
10.72
9.78
12.91
16.07
7.20
10.47 / 88.629
73.702
-47.533
-104.887
-212.484
-212.420
-57.339
-53.179
-58.361
0.005
0.004
-5.088
-6.806
7.273
6.288
-5.331
0.380
-0.277
-0.556
-0.576
-3.524
3.970
6.679
6.183
1.042
3.724
0.575
0.097
-0.086
0.077
-0.364
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8
8 / LR4/FT
LR4/FT
LR5/FT
LR1/BER
LR4/BER
LR1/LMS
LR1/LMS
LR1/LMS
LR4/LMS
LR5/LMS
LR6/LMS
LR4/NPB
LR4/NPB
LR5/NPB
LR6/NPB
LR6/NPB
LR1/LPB
LR1/LPB
LR1/LPB
LR4/LPB
LR5/LPB
LR1/DPB
LR5/DPB
LR1/ADM / Spring/2003
Autumn/2004
Spring/2005
Autumn/2005
Autumn/2004
Spring/2005
Autumn/2005
Autumn/2005
Autumn/2004
Spring/2005
Spring/2007
Spring/2003
Autumn/2004
Spring/2005
Spring/2007
Spring/2008
Spring/2005
Autumn/2005
Autumn/2005
Autumn/2004
Spring/2005
Spring/2005
Spring/2005
Autumn/2005 / 57.8 (48-55)
4.0 (0-13)
2.0 (0-6)
36.6 (31-46)
17.7 (12-22)
9.9 (5-18)
13.9 (10-26)
32.6 (29-37)
4.0 (0-10)
4.0 (2-6)
37.6 (34-47)
30.5 (5-37)
18.1 (0-23)
0.01 (0-5)
44.7 (42-48)
35.6 (33-42)
40.5 (38-47)
38.5 (34-45)
15.9 (9-24)
13.7 (0-18)
13.1 (6-19)
42.5 (35-51)
0.01 (0-3)
60.5 (41-68) / 57.8 (48-65)
4.0 (0-13)
1.4 (0-4.44)
35.6 (30-48)
17.7 (12-22)
12.8 (8-51)
15.9 (12-25)
32.0 (28-36)
4.0 (0-10)
2.9 (1-4)
61.8 (55-75)
30.5 (5-37)
18.1 (0-23)
0.01 (0-3)
73.7 (70-76)
57.8 (53-70)
40.3 (36-49)
37.4 (33-46)
17.4 (12-24)
13.7 (0-18)
9.7 (4-14)
43.2 (34-55)
0.01 (0-2)
65.3 (41-62) / 4.5
2.6
9.2
2.5
3.0
3.7
11.5
12.6
8.3
15.3
3.6
4.3
3.4
7.8
3.5
3.5
5.9
4.0
3.9
2.8
5.4
2.5
11.0
3.8 / 5.22
5.17
20.97
4.45
7.29
7.21
20.07
23.51
12.24
32.32
8.98
19.72
6.49
19.48
8.56
7.38
10.47
6.12
6.04
6.87
13.72
5.36
25.52
11.62 / -31.090
-87.827
-75.791
-0.004
0.003
-3.116
-8.026
-8.871
4.808
10.192
5.984
-0.712
-0.310
-0.578
-0.357
0.475
-3.107
-2.242
-3.744
3.397
1.324
-0.063
-0.180
-0.989
aSee Table 2 for explanation of traitsFT Flowering time (°C.D), LPB: Length of primary branches (cm), BER: Branch elongation rate (cm/°C.D), LMS: Length of main stem (cm), NPB: No of primary branches, DPB: Diameter of primary branches (mm)
bPA positive (negative) values indicates that the allele of the female parent induces a positive (negative) effect on alleles increase for eachthe trait, and negative values indicate that female parent alleles decrease for each trait