Supplemental Table 5. Primer Sequences

Supplemental Table 5. Primer sequences

All primer sequences are given in the 5’ to 3’ orientation.

Primers for inverted repeat construction:

Primers for PSuc2:PDS line RAGE and PSuc2:PDS line genotyping:

Genotyping of crosses to PSuc2:PDS:

Genotyping of crosses to PSuc2:PDS (cont).

Primers for sRNA detection and construction of riboprobe vectors:

Primers for low resolution genetic mapping:

Primers for low resolution genetic mapping (cont).

Primers for high resolution genetic mapping:

Below the marker name is given the restriction enzyme for CAPs markers or ‘indel’ is written if there is an insertion/deletion size polymorphism.

Chromosome 3:

Primers for high resolution genetic mapping:

Chromosome 3: (cont.)

Sequencing and RT-PCR of 24nt pathway genes:

Sequencing and RT-PCR of 24nt pathway genes (cont).

Primers for sequencing and genotyping of CLSY1:

Primers for sequencing and genotyping of CLSY1 (cont).

Primers for high molecular weight RNA gel blot analysis:

Primers for RT-PCR of PSuc2:PDS transcript: