Supplemental Table 1. Primer sequences for genes associated with lignin and secondary thickening.

Gene / Primer Sequence / Predicted function
MYB26/MS35 / 35_181L:GAGGGCTTTGGTCACCTGAAGA
35_1060R: GTCCACAAGAGATTGGCGACGA
NST1 / NST1F: TCATCCGACCGAGGAAGAGC
NST1R: GAAGCTCCTCCGACGGGACT / NAC domain TF associated with secondary thickening (Mitsuda et al., 2005)
NST2 / NST2F: TCACCCAACCGAGGAAGAGC
NST2R: CATGATCGCCACACGAGGAG / NAC domain TF associated with secondary thickening (Mitsuda et al., 2005)
XCIP / XCIPF: GAGGCTTCAGGAAGAGACTTCCAG
XCIPR: CACTTGGTCTTGGTAGGATATG / Cys endopeptidase, papain-type (associated autolysis tracheary elements)
(Funk et al., 2002)
IRX4 (CCR) / IRX4F: CGTTATCTCCTAGCCGAGAGTGCTC
IRX4R: TGCCATTTTCCACGGATTCTTGCGATGC / cinnamoyl-CoA reductase in lignin biosynthesis
(Jones et al., 2001)
IRX1 / IRX1F: GGTCTCCCATCTGCAACACTTG
IRX1R: GGTGGCGTATCTTCCATGTGC / Part of cellulose synthase complex specific for secondary cell wall thickening (Taylor et al., 1999; Jones et al., 2001)
IRX5 / IRX5F: GCTCAGTGTACCTCGCCAT
IRX5R: TTGGACGCCATTGCTGCTTA / Part of cellulose synthase complex specific for secondary cell wall thickening (Taylor et al., 1999; Jones et al., 2001)
IRX3 / IRX3-228F: TCCGGCGTGTAGACCTTGCTA
IRX3-R: GGGAACCACTTGGGGAACTGA / Part of cellulose synthase complex specific for secondary cell wall thickening (Taylor et al., 1999; Jones et al., 2001)
IRX8 / IRX8F: ACTATCGACGGCGATCCCTCT
IRX8R: GCCGCTGCGTTTATCGAGTG / Glycosyl transferase possible role in pectin synthesis
IRX13 / IRX13F: AGGTCCAACGAACATAACCGC
IRX13R: CAAACCCGAATCCAGTCCTCTC / Arabinogalactan
(Persson et al., 2005)
MYB32 / MYB32F: CCTAGATCCGCCGGTCTTCA
MYB32R: TCTCGTCCCCGAAATTTGCT / (Preston et al., 2004)
MYB61 / MYB61F: AGACTGCAGTTCTTCTTCTCTTTTACTGTT
MYB61R:
GTCGGATCCTCATTGTTTCAGTTTCTTCT / (Newman et al., 2004)
ANS / ANSF: ACCAAACCGCTATGTGAAGC
ANSR: ATTTAATGGTGACCCAGCCA / (Preston et al., 2004)
C4H / C4HF: TTCACCGGATCTAACCAAGG
C4HR: CGTTGATTTCTCCCTTCTGC / (Preston et al., 2004)
DFR / DFRF: GTCGGATCCAGTTTCATCGT
DFRR: CTTCATTGTTTGGGTTGCCT / (Preston et al., 2004)
COMT / COMTF: TCAGCGTATCCAAGAGAGCA
COMTR: GCGTGATACCATCTCCAATG / (Preston et al., 2004)
PAL2 / PAL2F: GAGGCAGCGTTAAGGTTGAG
PAL2R: TTCTCGGTTAGCGATTCACC / (Preston et al., 2004)
IRX12 / LaccaseF GGTGGATGGGTCGTCATGAGATTC
LaccaseR CGTGGCGTGATGTTGATATGTCGCCC / Putative laccase involved in lignin biosynthesis
(Brown et al., 2005)
At3g62160 / 3G62160F: AACCGCTTGTCCAAATGCAGGT
3g62160R: CGATTTCTCCGACCGCGGTA / Transferase family protein (Mitsuda et al., 2005).
AtHB-8 / AtHB8F: TGTGTTGCTCACTCAAGGCCTTA
AtHB8R: TGTCGAAGATCTTGTCGAGAGTGA / Procambial cell marker, Homeobox-Leu Zipper transcription factor (Mitsuda et al., 2005).

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