Supplemental Table 1. Oligonucleotides Used for Cloning and Qrt-PCR

Supplemental Table 1. Oligonucleotides used for cloning and qRT-PCR

Gene / 5'- 3' primer sequences / accession number
Arbp / NM_131580
Arp_for / CATCCAGCAGGTGTATGATAACGG
Arp_rev / CTCACACCCTCCAGGAACCTC
β-Actin / NM_181601
Beta-Actin_for / CGAGCTGTCTTCCCATCCA
Beta-Actin_rev / TCACCAACGTAGCTGTCTTTCTG
Cygb1 / NM_152952
Cygb1_for / GTGCTGGTCAGGTTTTTCACAA
Cygb1_rev / TGTGTTGAGAGCGTTCAAGAC
Cygb2 / NM_001024224
Cygb2_for / ACATGACCCAGAGAAAGTATCATCTG
Cygb2_rev / CTCTCCAAACTCCTCTGCAAG
EF1α / NM_131263
EF1alpha_for / CTGGAGGCCAGCTCAAACAT
EF1alpha_rev / ATCAAGAAGAGTAGTACCGCTAGCATTAC
GbX / NM_001012261
GbX_for / GAAGTTCTCAACCGTCTGTTCAA
GbX_rev / GCTTCCCGCCAGATCACCCT
Hbα (embr.) / NM_182940
embr_HbA_for / GCCAAYTTCAAGATYCTGTCMCAC
embr_HbA_rev / GTACTTCTCAGASAKRGCCAG
Hbβ (embr.) / NM_182940
embr_HbB_for / CTSCAYGTRGATCCYGACAACT
embr_HbB_rev / TGMTACTGTCTKYMCAGAGCRGA
Hbα / NM_131257
HbAa_for / CTGATACGGACAAGGCTGTTGT
HbAa_rev / AGACGGTCAGCATTCTGGCGA
Hbβ / NM_001013027
DreHbBa_for / ATGGTTGAGTGGACAGATGC
DreHbBa_rev / TACACGATCAGACATCTGGATA
HbαX / NM_001082834
HbAx_for / TAGCAGGTTCCATGCCTACC
HbAx_rev / TCATCGGAACTTCTCGGCTA
His2h3c1 / XM_686806
HistonH23c1_for / GGTCAGGTTCCATGCCTACC
HistonH23c1_rev / TCATCGGAACTTCTCGGCTA
Ldha / NM_131246
LDHA_for / ATTGGAATGTCTGTAGCTGACCTGT
LDHA_rev / TACACCATGCATTCCCTTGACCAAAG
Mb / NM_200586
Mb_for / GAAGTTCTCAACCGTCTGTTCAA
Mb_rev / GCTTCCCGCCAGATCACCCT
Ngb / NM_131853
Ngb_for / AAGGTGATGTTAGTGATTGATGCAG
Ngb_rev / GGACTCTCCAACCAAAGCGAA
Ppia / NM_199957
CP_for / TCACACTGAAACACGGAGGCA
CP_rev / GCTTACCGTCCAGCCAGTTG

Supplemental Table 2. Sequences used for phylogenetic inference of Hb. Protein database accession numbers are given where avialable.

Abbreviation / Species / accession number
AanHbα / Anguilla anguilla / P80945
AanHbα2 / Anguilla anguilla / P80726
AmiHbα1 / Anarhichas minor / P83270
AmiHbα2 / Anarhichas minor / P83271
CauHbα / Carassius auratus / P02018
CauHbα1 / Carassius auratus / AF528157
CauHbα2 / Carassius auratus / unpublished
CcaHbα1 / Cyprinus carpio / M25643
CcaHbα2 / Cyprinus carpio / AF528156
CcaHbα3 / Cyprinus carpio / AB004739
CcaHbα4 / Cyprinus carpio / AB004735
CcaHbα5 / Cyprinus carpio / AB004738
CcaHbα6 / Cyprinus carpio / AB004736
CcaHbα7 / Cyprinus carpio / AB004734
CcaHbα8 / Cyprinus carpio / AB004737
CclHbα / Catostomus clarkii / P02017
CcoHbα / Conger conger / P83479|
CidHbα / Ctenopharyngodon idella / AF528197
CkuHbα / Chelidonichthys kumu / P80270
CmaHbα / Cygnodraco mawsoni / P23016
DreHbαa / Danio rerio / NM_131257
DreHbαe / Danio rerio / NM_182940
DreHbαx / Danio rerio / AL915033
DreHbαz / Danio rerio / BC042321
EelHbα / Electrophorus electricus / P14520
EluHbαx / Esox lucius / BT079579
FheHbαx / Fundulus heteroclitus / EV459084
GacHbα1 / Gymnodraco acuticeps / P29623
GdoHbα / Gadus morhua / X98195
GmoHbα3 / Gadus morhua / ACV69832
HcoHbα / Hippocampus comes / AY437396
HliHbα / Hoplosternum litorale / P82315
HpoHbα / Heterodontus portisjacksoni / P02021
HsaHbα / Homo sapiens / NM_000558
LxaHbα / Leiostomus xanthurus / P56250
ManHbα / Misgurnus anguillicaudatus / AF528198
MmuHbα / Mus musculus / NM_008218
NcoHbα / Notothenia coriiceps / P16308
NcoHbα1 / Notothenia coriiceps / U09186
NcoHbα11 / Notothenia coriiceps / P10777
NtaHbα / Notothenia angustata / P29624
OlaHbαa / Oryzias latipes / AB080119
OlaHbαe / Oryzias latipes / AB026052
OlaHbαe2 / Oryzias latipes / AB080117
OlaHbαx / Oryzias latipes / BAC20303
OmyHbα1 / Oncorhynchus mykiss / P02019
OmyHbα4 / Oncorhynchus mykiss / P14527
OmyHbαe / Oncorhynchus mykiss / AB015449
OmyHbαI / Oncorhynchus mykiss / D88113
OmyHbαIV / Oncorhynchus mykiss / D88114
OmyHbαx / Oncorhynchus mykiss / BX081300
PboHbα / Pagothenia borchgrevinki / P82990
PdaHbα / Paramisgurnus dabryanus / AF528199
PflHbαx / Platichthys flesus / EC379099
RnoHbα / Rattus norvegicus / NM_013096
RruHbαx / Rutilus rutilus / EG539205
SacHbα / Squalus acanthias / P07408
SchHbαx / Siniperca chuatsi / GR476179
SquHbαa / Seriola quinqueradiata / AB034639
SquHbαb / Seriola quinqueradiata / AB034640
SsaHbα / Salmo salar / X14431
TbeHbα / Trematomus bernachii / P80043
TneHbα1 / Trematomus newnesi / P45719
TneHbα2 / Trematomus newnesi / P45718
TruHbα1 / Takifugu rubripes / CA330538
TruHbα2 / Takifugu rubripes / AY016024
TruHbα3 / Takifugu rubripes / AY170464
TruHbα4 / Takifugu rubripes / AA061494
TthHbα1 / Thynnus thynnus / AB093569
TthHbα2 / Thynnus thynnus / AB093570

Supplemental figures

Supplemental Fig. 1. mRNA copy numbers of Hbα (white columns) and Hbβ (black columns) during zebrafish development. Bar diagram shows qRT-PCR results of different developmental stages from unfertilized eggs (0 hpf) to sexually mature adult zebrafish at 54dpf. The y-axis has a logarithmic scale. Bars represent standard errors of the means.* indicates missing data.

Supplemental Fig. 2. mRNA copy numbers of embryonic Hbα (white columns), HbαX (grey columns) and embryonic Hbβ (black columns) during zebrafish development. Bar diagram shows qRT-PCR results of different developmental stages from unfertilized eggs (0 hpf) to sexually mature adult zebrafish at 54dpf. The y-axis has a logarithmic scale. Bars represent standard errors of the means.* indicates missing data.

Supplemental Fig. 3. mRNA copy numbers of embryonic Cygb 1 (white columns), Cygb 2 (grey columns) and GbX (black columns) during zebrafish development. Bar diagram shows qRT-PCR results of different developmental stages from unfertilized eggs (0 hpf) to sexually mature adult zebrafish at 54dpf. The y-axis has a logarithmic scale. Bars represent standard errors of the means.* indicates missing data.

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