Standard Operating Procedures
Laboratory Specific
Chemical: Trinitrobenzene
Please fill out the form completely. Print a copy and insert into your
Laboratory Safety Manual and Chemical Hygiene Plan.
Refer to instructions for assistance.
______
Department:______Date when SOP was written:______
Date when SOP was approved by the lab supervisor: ______
Principal Investigator:______
Internal Laboratory Safety Coordinator/Lab Manager:______
Laboratory Phone:______Office Phone:______
Emergency Contact:______
(Name and Phone Number)
Location(s) covered by this SOP:______
(Building/Room Number)
______
Type of SOP: Process Hazardous Chemical Hazardous Class
Purpose
Trinitrobenzene is used primarily as a high explosive for commercial mining and military use. Some other uses include a narrow-range pH indicator, an agent to vulcanize natural rubber, and a mediating agent to mediate the synthesis of other explosive compounds. Trinitrobenzene is a nitrated benzene-derivative that is classified as a high explosive, being moderately explosive in liquid form and extremely explosive in its dry powder form. It has a clear to light yellow sludgy appearance. It will detonate under strong shock. High temperatures, whether by sudden heating of any quantity, or by the accumulation of heat when large quantities are burning, will also cause detonation.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 99-35-4
Class: Potentially Explosive
Molecular formula: C6H3N3O6
Melting Point: 123.2 °C, 396 K
Boiling Point: 315 °C, 588 K
Appearance: solid
Potential Hazards/Toxicity
Oral LD50:
LD50 Oral - rat - 275 mg/kg
Remarks: Behavioral:Somnolence (general depressed activity). Behavioral:Rigidity (includes catalepsy). Lungs, Thorax, or Respiration:Dyspnea.
Personal Protective Equipment (PPE)
Respiratory protection:
Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N100 (US) or type P3 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU).
Hand protection:
Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands.
Eye protection:
Face shield and safety glasses Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU).
Skin and body protection:
Complete suit protecting against chemicals, Flame retardant antistatic protective clothing, The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace.
Hygiene measures:
Avoid contact with skin, eyes and clothing. Wash hands before breaks and immediately after handling the product.
Engineering Controls
· All operations should be carried out in a certified chemical fume hood, glovebox, or a ducted Biosafety cabinet to keep airborne level below recommended exposure limits.
· Laboratory rooms must be at negative pressure with respect to the corridors and external environment. The laboratory/room door must be kept closed at all times.
First Aid Procedures
Inhalation: If inhaled, remove to fresh air. CALL A PHYSICIAN IMMEDIATELY.
Ingestion: Aspiration hazard. If swallowed, DO NOT INDUCE VOMITING. Never give anything by mouth to an unconscious person. Get medical attention immediately. If vomiting occurs, keep head below hips to prevent aspiration into lungs.
Skin Contact: In case of contact, immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Call a physician immediately.
Eye Contact: Immediately flush eyes with plenty of water for at least 15 minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.
Special Handling and Storage Requirements
Precautions for safe handling
· Avoid contact with skin and eyes. Avoid formation of dust and aerosols.
· Provide appropriate exhaust ventilation at places where dust is formed. Avoid shock and friction. Keep away from sources of ignition - No smoking.
Conditions for safe storage
· Protect against physical damage. Store in a cool, dry well-ventilated location, away from any area where the fire hazard may be acute. Outside or detached storage is preferred.
· Separate from incompatibles. Do not store near other chemicals (especially other oxidizers), near sources of heating, or places where rapid temperature changes may occur.
Spill and Accident Procedure
Chemical Spill Dial 911 and x59797
Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.
Medical Emergency Dial 911 or x52111
Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.
Decontamination/Waste Disposal Procedure
1. Whatever cannot be saved for recovery or recycling should be handled as hazardous waste.
2. All solid trinitrobenzene contaminated waste shall be disposed of into clear double bag waste specifically designated for trinitrobenzene waste. Examples of solid trinitrobenzene waste material include gloves, pipette tips, and paper towels.
3. Once the waste container is full, dispose of according to the UCLA EH&S hazardous waste guidelines.
Material Safety Data Sheet (MSDS) Location
(State the location of MSDS)
Hardcopy or electronic copy must be available.
Online MSDS can be accessed at http://msds.ehs.ucla.edu.
Protocol/Procedure
(Add specific description of procedure.)
Note: Any deviation from this SOP requires written approval from PI.
Documentation of Training (signature of all users is required)
ü Prior to conducting any work with trinitrobenzene, designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.
ü The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of trinitrobenzene MSDS provided by the manufacturer.
ü The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last two years.
I have read and understand the content of this SOP:
Name Signature Date
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