S1. Summary of the 16S Rrna Bacterial Sequences Identified in Tunisian Hot Springs

S1. Summary of the 16S rRNA bacterial sequences identified in Tunisian hot springs

Sample / Phylogenic affiliation / Type sequence / Closest published relative (Blast) / Sequence similarity (%)
1 / Alphaproteobacteria / P2RS50 (5) / Oceanibaculum indicum strain P24 (EU656113 ) from deep sea environment / 98
Alphaproteobacteria / P2RS58 (5) / Clone 6C233071 (EU805086 ) from seawater, Thalassospira xiamenensis strain M-5 (AY189753) / 99
Gammaproteobacteria / P2RS79 (3) / Marinobacterium litorale strain IMC1877 (DQ917760) / 94
Alphaproteobacteria / P2RS60 (1) / Clone JP57 (AY007683) from surface water of costal North sea / 97
Alpha proteobacteria / P2RS76 (5) / Parvibaculum lavamentivorans (NR_029105) / 99
Alphaproteobacteria / P2RS75 (15) / Uncultured Parvibaculum clone Rc47 (EU375050) from shoreline environments / 99
2A / Synergistetes / P3RS67 (4) / Uncultured Synergistes sp. clone D004011B05 (EU721758 ) from Alaskan mesothermic petroleum reservoir / 98
Alphaproteobacteria / P3RS53 (3) / Rhizobiales bacterium RDX 10 (EU907874 ) from contaminated soil / 99
Alphaproteobacteria / P3RS77 (2) / Clone RDX 10 (EU907874 ) from contaminated soil, Pleomorphomonas oryzae strain (AB159685) 93% / 93
Alphaproteobacteria / P3RS54 (1) / Clone RDX 10 (EU907874 ) from contaminated soil, / 98
Gammaproteobacteria / P3RS75 (2) / Pseudomonas. sp CF14-10 (FJ170038) from sediments of South China sea / 99
Deltaproteobacteria / P3RS80 (18) / Desulfopila aestuarii (AB110542) from an estuarine sediment in Japan / 96
Deltaproteobacteria / P3RS57 (4) / Clone CM16 (AM910068) rhizosphere soil from rice field / 90
2B / Gammaproteobacteria / P9B2BRS44 (1) / Vibrio navarrensis (X74715) / 99
Deltaproteobacteria / P9B2BRS47 (10) / Desulfopila aestuarii (AB110542) from an estuarine sediment in Japan / 97
Deltaproteobacteria / P9B2BRS9 (1) / Clone 661244 (DQ404791) from contaminated sediment / 86
Deltaproteobacteria / P9B2BRS10 (1) / Uncultured marine sulfate-reducing bacterium clone BznS334 (EU047541 ) / 88
P9B2BRS46 (3) / Clone 3uPen_H04 (EU628131) from water column from fish pens / 97
Firmicutes / P9B2BRS2 (1) / Clone MAT-CR-M2-C02(EU245492) hypersaline microbial mat, Clostridiisalibacter paucivorans (EF026082) 91% / 91
Firmicutes / P9B2BRS12 (1) / Clone QEDV3BE06 (CU919076 ) from mesophilic anaerobic digester / 97
Firmicutes / P9B2BRS14 (2) / Halobacillus sp. SR5-3 (AB185150) from fish sauce in Thailand / 96
Alphaproteobacteria / P9B2BRS16 (1) / Rhizobium sp. TCK (AJ294417) from Indian soils / 93
3 / Nitrospirae / P10B3RS8 (2) / Clone pIR3BB12 (AY354168)from Rainbow hydrothermal vent sediments / 90
Deltaproteobacteria / P10B3RS3 (2) / Uncultured clone Dan_Bac69 (FN356276) oil production water, Desulfoglaeba alkanexedens (DQ303457) 92% / 92
Firmicutes / P10B3RS15 (1) / Anoxybacillus kamchatkensis (AF510985) from Kamchatka hot spring / 98
4 / Gammaproteobacteria / P5b4RS13 (3) / Clone SN166 (EU735702) from pristine soil in Jidong oilfield / 91
Gammaproteobacteria / P5b4RS20 (20) / Pseudomonas stutzeri (AJ006107) / 98
Gammaproteobacteria / P5b4RS43 (1) / Clone RH.204i-50-16 (DQ458432) from high salinity industrial wastewater / 98
5 / Firmicutes / P2RS46 (1) / Uncultured Desulfosporosinus sp. clone X9Ba86 (AY607216) from anoxic rice field soil / 86
Firmicutes / P2RS40 (1) / Desulfosporosinus meridiei strain S10 (NR_024933) from a shallow aquifer contaminated with gasoline / 98
Firmicutes / P2RS8 (3) / Desulfosporosinus meridiei strain S10 (NR_024933) from a shallow aquifer contaminated with gasoline / 93
Firmicutes / P2RS32 (2) / Desulfosporosinus auripigmenti strain OREX-4 (NR_025551) from Surface sediments from UpperMysticLake / 97
Firmicutes / P2RS33 (4) / Clone D47 (AM713394) from coal-mining lake sediment / 97
Firmicutes / P2RS12 (4) / Bacillus sp. GB02-14B (DQ078995) from hydrothermal sediments and plumes / 97
Firmicutes / P2RS3 (8) / Clone BSV51(AJ229203) from anoxic rice puddy soil / 98
Firmicutes / P2RS25 (4) / Uncultured bacterium clone D10 from nitrobenzene-contaminated river sediments / 96
P2RS19 (1) / Caldicellulosiruptorkristjanssonii(I77R1B) (NR_025279) from biomat sample of an Icelandic hot spring / 96
Firmicutes
Gammaproteobacteria / P2RS23 (3) / Uncultured bacterium clone HH_aai33d01 (EU775655)from Hedgehog feces / 93
Firmicutes / P14b5RS45 (4) / Clostridium from anoxic bulk soil of rice paddy microcosms strain XB90 (AJ229234) / 98
Firmicutes / P14b5RS4 (2) / Clone BSV51(AJ229203) from anoxic rice paddy soil / 95
Firmicutes / P14b5RS11 (1) / Clone D41S30C32 (FM956920) from rice field soil / 95
Firmicutes / P14b5RS48 (1) / Bacillus sp. GB02-21B/C (DQ078998) from hydrothermal sediments and plumes / 99
Firmicutes / P14b5RS26 (2) / Sedimentibacter.sp. BRS2 (AY221992) chloroethene-contaminated aquifer / 99
Deinococcus-Thermus / P14b5RS44 (1) / Thermus thermophilus (X07998 ) / 93
Deinococcus-Thermus / P14b5RS8 (3) / Thermus sp. RH-0406 (AY731827) from hot springs in China / 100
Firmicutes / P2RS41 (1) / Bacillus smithii strain R-7170 (AY373319) from raw milk / 90
candidate division OP9 / P14b5RS47 (2) / Candidate division OP9 clone OPB47 (AF027082) from yellowstone hot spring / 94
Firmicutes / P14b5RS21 (3) / Clone D14R15C52 (FM956751) from rice field soil / 97
Firmicutes / P2RS11 (1) / Bacillus sp. PL-7 (AF326371) from marine sediments / 99
6 / P3RS4 (4) / Clone: ODP1230B3.31 (AB177196) from methane hydrate bearing subseafloor sediment / 78
Firmicutes / P3RS28 (1) / Clone D47 (AM713394) from coal-mining lake sediment / 96
Firmicutes / P3RS31 (1) / Clone Gly030-8A (AY524569) from acidic subsurface sediment contaminated with uranium (VI) / 88
Firmicutes / P3RS23 (8) / Thermolithobacter carboxydivorans strain R1 (DQ095862) from sediment and water of Calcite Spring YNP / 85
Firmicutes / P3RS8 (2) / Clone TfC20H44 (EU362204 ) from tidal flat sediment / 85
Nitrospirae / P3RS26 (1) / Clone D14 (DQ424917) from fuel cell enriched with artificial wastewater, Thermodesulfovibrio islandicus / 92
Thermotogae / P3RS16 (5) / Uncultured Thermotogales bacterium clone B17_otu14 (DQ097679) from oil field / 97
Green nonsulfur bacteria / P3RS44 (1) / Yellowstone hot spring clone OpB11 (AF027032) / 98
7 / Aquificales / P5b7RS69 (8) / Hydrogenobacter subterraneus (NR_024729 ) from subsurface geothermal water / 99
9 / Firmicutes / P7b9RS61 (3) / Uncultured bacterium clone AKIW790 (DQ129269) from urban aerosol / 94
Firmicutes / P7b9RS67 (20) / Clone D6-41(AB331494) from aerated pig manure slurry / 93
P7b9RS81 (3) / Clone D14R15C24 (FM956737) from rice field soil / 91
Firmicutes / P7b9RS64 (10) / Clone AKIW1134 (DQ129330) from urban aerosol / 96
Firmicutes / P7b9RS63 (1) / Bacillus niacini (NR_024695) from soil / 97
P7b9RS90 (1) / Clone D14R15C24 (FM956737) from rice field soil / 86
Deinococcus-Thermus / P7b9RS95 (1) / Thermus sp. SPS-217 (AJ871174) from hot spring water / 90
10 / Alpha proteobacteria / P7b10RS8 (6) / Clone AKYG1729 (AY921978) from farm soil adjacent to a silage storage bunker / 94
Bacteroidetes / P7b10RS1 (3) / Clone SSE_L1_H05 (EU635948) from hot spring, Rhodothermus marinus (94%) / 96
11 / Gammaproteobacteria / P1B1G34 (4) / Thiofaba tepidiphila (AB304258 ) from hot spring / 95
Betaproteobacteria / P1B1G30 (2) / Clone 390 (EF188435) from AltamiraCave / 91
Gammaproteobacteria / P1B1G21 (1) / Uncultured Stenotrophomonas sp. clone GI6-1-A05 (FJ193196) from clean room surface / 94
Bacteroidetes / P1B1G18 (2) / Uncultured Bacteroidetes bacterium clone Dover58 (AY499777) from marine sediment / 90
Deinococcus-Thermus / P1B1G15 (1) / Meiothermus timidus strain RQ-10 (AJ871169) from hot spring water / 96
12 / Alphaproteobacteria / P16RS43 (1) / Brevundimonas sp. V52 (DQ778038) from dune grassland soil / 92
Firmicutes / P16RS82 (1) / Paenibacillus borealis strain KK19 (NR_025299) / 97
Gammaproteobacteria / P16RS1 (7) / Pseudomonas sp. CF14-10 (FJ170038) from the the sediments of the South China sea / 99
Gammaproteobacteria / P16RS26 (1) / Uncultured Pseudomonas sp. clone 3-A (EU305582) from wastewater plant / 99
Gammaproteobacteria / P16RS70 (2) / Pseudomonas sp. CF14-10 (FJ170038) from the the sediments of the South China sea / 99

S2.Summary of the 16S rRNA archaeal gene sequences identified in Tunisian hot springs

Sample / Phylogenic affiliation / Type sequence / Clones number / Closest published relatives / similarity (%)
1 / Crenarchaeota / P4E1RS1 / 15 / Clone ERB-A-A2 (EU490282) from hot mineral soils / 99
Crenarchaeota / P4E1RS6 / 16 / Clone Elev_16S_arch_1892 (EF022539) from soil affected by elevated atmospheric CO2 / 97
Crenarchaeota / P4E1RS32 / 12 / Clone Elev_16S_988 (EF023096 ) soil affected by elevated atmospheric CO2 / 99
2A / Euryarchaea, Halobacteria / P1A2RS32 / 1 / Clone WN-FSA-155 (DQ432495) from alkaline, hypersaline lake, Egypt / 93
Crenarchaeota / P1A2RS46 / 1 / Clone AT_R003 (AF419648 ) from hydrothermal sediments, GuaymasBasin / 90
Crenarchaeota / P1A2RS38 / 5 / Clone HTA-G6 (AF418938) from freshwater reservoir / 100
Euryarchaea, Halobacteria / P1A2RS16 / 8 / haloarchaeon clone TX4CA_36 5 (EF690591) from alkaline-saline soil (Mexico) / 93
Euryarchaea,Halobacteria / P1A2RS1 / 10 / Clone CP-A-165 (DQ521200) from Cold Saline Perennial Springs of the Canadian High Arctic / 92
Euryarchaea,Halobacteria / P1A2RS28 / 7 / Clone Au-Fav1-Arch31 (EU377076) from surface mucus of corals , Halococcus sp. HPA-87(EF150811) 95% / 96
2B / Euryarchaea,Halobacteria / P9a2BRS94 / 1 / Uncultured haloarchaeon clone TX4CA_56 from from alkaline-saline soil (Mexico), Halalkalicoccus jeotgali (EF077632) / 92
Euryarchaea,Halobacteria / P9a2BRS60 / 1 / Uncultured Halobacteriales archaeon ZAR23 (AY341288) from low-salt, sulfide- and sulfur-rich spring / 91
Crenarchaeota / P9a2BRS78 / 1 / Clone HMMVCen-19 (AJ579326) from Haakon Mosby mud volcano / 94
3 / Crenarchaeota / P1a3RS91 / 41 / Clone AK8 (AY555814) from Bor Khlueng hot spring in Thailand / 95
4 / Euryarchaea,Methanosarcinales / P6a4RS35 / 10 / Clone TP123 (EF198052) from phenol-degrading methanogenic consortia, Methanosaeta thermophila PT (AB071701) / 97
Crenarchaeota / P6a4RS38 / 7 / Clone AK8 from Bor Khlueng hot spring in Thailand / 98
Crenarchaeota (Group (MCG))* / P6a4RS7 / 4 / Uncultured crenarchaeote clone: MDS-r-E06 ( AB353218) from mesophilic digested sludge / 98
Crenarchaeota / P6a4RS26 / 2 / Clone HL1env.09 (EU239997) from HeartLake 1 hot spring sediment / 89
Crenarchaeota / P6a4RS13 / 3 / Clone: YS18As55 (AB329818) from iron-silica-rich microbial mats,deep-sea hydrothermal field / 91
Crenarchaeota / P6a4RS47 / 2 / Uncultured Crenarchaete clone OPPDO23 (AY861957) from Yellowstone geothermal systeme (Opsidian Pool) / 92
Euryarchaeota, Methanomicrobiales / P6a4RS43 / 1 / Clone 1B7 (AY426477 ) from anaerobic bioreactor , Methanospirillum hungatei (M60880) / 88
5 / Crenarchaeota (MCG) / P10a5RS55 / 4 / Uncultured crenarchaeote clone: pIta-HW-17 (AB301869) from submarine hydrothermal system, Japan / 88
Crenarchaeota / P10a5RS49 / 5 / Clone SSW_L4_G06 (EU635916) from Great Basinhot springs / 94
Euryarchaeaota / P10a5RS68 / 4 / Archaeon enrichment culture from petroleum reservoirclone PW45.1(EU573153) /Archaeoglobus fulgidus (VC-16) (Y00275 ) / 91
Crenarchaeota / P10a5RS63 / 3 / Uncultured crenarchaeote clone Y5x (DQ417463 ) from see floor / 85
Crenarchaeota (MCG) / P10a5RS61 / 6 / Uncultured crenarchaeote,clone: HAuD-LA42 (AB113634) from subsurface geohermal water / 95
Crenarchaeota (MCG) / P10a5RS87 / 1 / Uncultured crenarchaeote,clone: HAuD-LA30 (AB113632) from subsurface geohermal water / 90
Crenarchaeota / P14A5RS49 / 1 / Uncultured crenarchaeote clone OPPD030 (AY861963 ) from Yellowstone geothermal systeme (Opsidian Pool) / 96
Crenarchaeota / P14A5RS55 / 2 / Uncultured crenarchaeote clone OPPD030 (AY861963 ) from Yellowstone geothermal systeme (Opsidian Pool) / 97
Crenarchaeota (MCG) / P14A5RS61 / 4 / Uncultured crenarchaeote clone: pIta-HW-17 (AB301869) from submarine hydrothermal system, Japan / 88
Crenarchaeota (MCG) / P14A5RS80 / 3 / Uncultured crenarchaeote clone: pIta-HW-1 (AB301857 ) from submarine hydrothermal system, Japan / 95
Crenarchaeota / P14A5RS65 / 4 / Uncultured crenarchaeote clone OPPD023 (AY861957 ) from Yellowstone geothermal systeme (Opsidian Pool) / 95
Crenarchaeota / P14A5RS64 / 7 / Uncultured crenarchaeote clone HL1env.09 (EU239997) from HeartLake 1 hot spring sediment / 97
Crenarchaeota, Thermoproteales / P14A5RS89 / 2 / Clone Hverd033N (DQ441512) from Icelandic hot springs, Thermoproteus neutrophilus (AB009618) / 94
Crenarchaeota / P14A5RS63 / 1 / Uncultured crenarchaeote clone OPPD002 (AY861940 ) from Yellowstone geothermal systeme (opsidian Pool) / 96
Crenarchaeota / P14A5RS59 / 2 / Uncultured archaeon clone SSE_L4_D02 (EU635907) from Great Basinhot springs / 95
6 / Crenarchaeota (SCG) ** / P4E6RS53 / 44 / Uncultured crenarchaeote clone clone: HAuD-LA33 (AB113633) from subsurface geothermal water / 99
Crenarchaeota / P4E6RS91 / 1 / Clone Elev_16S_arch_1892 (EF022539) from soil affected by elevated atmospheric CO2 / 98
7 / Crenarchaeota / P8a7RS64 / 38 / Uncultured archaeon clone AK8 (AY555814) from Bor Khlueng hot Spring in Thailand / 95
8 / Euryarchaea,Halobacteria / P8a8RS39 / 1 / Uncultured Halobacteria archaeon clone Y11A (EF106639 ) from hypersaline evaporites / 98
Euryarchaea,Halobacteria / P8a8RS5 / 2 / Haloferax larsenii strain ZJ203 (DQ458847 ) from solar saltern / 94
Euryarchaea,Halobacteria / P8a8RS30 / 5 / Clone ss007 (AJ969930) from saline soil / 96
Crenarchaeota / P8a8RS17 / 15 / Uncultured archaeon clone AK8 (AY555814) from Bor Khlueng hot spring in Thailand / 95
Euryarchaea,Halobacteria / P8a8RS47 / 1 / Haloarchaeon clone 9AH (AY292397) from estuarine sediments / 98
Crenarchaeota / P8a8RS35 / 2 / Uncultured archaeon clone CPA42 (DQ521187) from sediment of cold hypersaline sulfidic spring,Arctic / 94
Euryarchaea,Halobacteria / P8a8RS19 / 3 / haloarchaeon 8AHG (AY292395) from estuarine sediments / 94
Euryarchaea,Halobacteria / P8a8RS43 / 1 / Haloarchaeon CSW4.03.5 (EF127644) from water from a crystallizer pond, Australia , Natronomonas pharaonis (D87971)92% / 93
Euryarchaea,Halobacteria / P8a8RS38 / 1 / uncultured archaeon clone ss_032a (AJ969845) from saline soil / 94
Euryarchaea,Halobacteria / P8a8RS37 / 1 / Clone ss007 (AJ969930) from saline soil, Halorhabdus tiamatea strain SARL4B (EF127229 ) 94% / 95
Euryarchaea,Halobacteria / P8a8RS40 / 1 / Uncultured haloarchaeon clone TX4CA_56 (EF690611) from alkaline-saline soil (Mexico) / 92
9 / Crenarchaeota / P2A9RS11 / 5 / Clone Elev_16S_arch_1892 (EF022539) from soil affected by elevated atmospheric CO2 / 97
Euryarchaeaota;Halobacteria / P2A9RS20 / 2 / Uncultured Halobacteriales archaeon ZAR23 (AY341288) from low-salt, sulfide- and sulfur-rich spring / 94
10 / Euryarchaeaota / P6a10RS93 / 27 / Uncultured archaeon clone SAGMA-J (AB050214) from deep South African gold mines / 95
11 / Crenarchaeota / P3AG24 / 2 / Clone ArcC-s_cB07 (EU307056) from soil samples / 88
12 / Crenarchaeota / P1A3H2 / 10 / Clone ArcC-s_cB07 (EU307056) from soil samples / 91
Crenarchaeota / P1A3H12 / 3 / Uncultured archaeon AT_R003 from hydrothermal sediments, GuaymasBasin / 91