Responses of bacterial and fungal communities to an elevation gradient

in a subtropical montane forest of China

Han Meng, Ke Li, Ming Nie, Jia-Rong Wan, Zhe-Xue Quan, Chang-Ming Fang,

Jia-Kuan Chen, Ji-Dong Gu and Bo Li

M Nie, JR Wan, JK Chen, B Li

Centre for Watershed Ecology, Institute of Life Science and Key Laboratory of Poyang Lake Environment and Resource Utilization, Nanchang University, Nanchang 330031, P.R. China

H Meng, K Li, M Nie, ZX Quan, CM Fang, JK Chen, B Li

School of Life Sciences, Fudan University, Shanghai 200433, P.R. China

JD Gu

School of Biological Sciences, The University of Hong Kong, Pofulam Road, Hong Kong SAR, P.R. China

Table S1 ∫-libshuffanalysis for bacteria from different elevations of forest soils

Comparison / dCXYScore
HE-LE / 0.000206
LE-HE / 0.001747
HE-ME / 0.000441
ME-HE / 0.000486
LE-ME / 0.000902
ME-LE / 0.000231

LE: low elevation; ME: medium elevation; HE: high elevation.

Table S2 Comparison of soil bacterial diversity from high elevation through pyrosequencing and clone library methods

Phylum / Class / Order / Family / Genus / High Elevation
Pyrosequencing / % / Clone library / %
Acidobacteria / Acidobacteria / Acidobacteriales / Acidobacteriaceae / Acidobacterium / 56 / 4.09 / 0 / 0
Candidatus_Koribacter / 66 / 4.82 / 10 / 12.5
Unclassified / 52 / 3.8 / 0 / 0
Candidatus_Solibacter / 13 / 0.95 / 0 / 0
Edaphobacte / 21 / 1.53 / 1 / 1.3
Unclassified / Unclassified / 88 / 6.43 / 22 / 27.5
Actinobacteria / Actinobacteria / Actinomycetales / Catenulisporineae / Actinospica / 1 / 0.07 / 0 / 0
Corynebacterineae / Mycobacterium / 20 / 1.46 / 0 / 0
Unclassified / 2 / 0.15 / 0 / 0
Corynebacterium / 2 / 0.15 / 0 / 0
Gordonia / 1 / 0.07 / 0 / 0
Micromonosporineae / Unclassified / 1 / 0.07 / 0 / 0
Propionibacterineae / Propionibacterium / 3 / 0.22 / 0 / 0
Streptomycineae / Kitasatospora / 1 / 0.07 / 0 / 0
Frankineae / Acidothermus / 51 / 3.73 / 0 / 0
Unclassified / 3 / 0.22 / 0 / 0
Solirubrobacterales / Conexibacteraceae / Conexibacter / 10 / 0.73 / 1 / 1.3
Solirubrobacteraceae / Solirubrobacter / 18 / 1.31 / 0 / 0
Acidimicrobiales / Acidimicrobineae / Unclassified / 83 / 6.06 / 0 / 0
Unclassified / 23 / 1.68 / 1 / 1.3
Bacteroidetes / Sphingobacteria / Sphingobacteriales / Chitinophagaceae / Segetibacter / 2 / 0.15 / 0 / 0
Unclassified / 14 / 1.02 / 0 / 0
Chitinophaga / 2 / 0.15 / 0 / 0
Sphingobacteriaceae / Mucilaginibacter / 3 / 0.22 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Unclassified / 3 / 0.22 / 0 / 0
Unclassified / 2 / 0.15 / 0 / 0
Candidate_division_OP10 / Unclassified / 1 / 0.07 / 1 / 1.3
Candidate_division_TM6 / Unclassified / 1 / 0.07 / 0 / 0
Candidate_division_TM7 / Unclassified / 8 / 0.58 / 0 / 0
Chloroflexi / Unclassified / 33 / 2.41 / 0 / 0
Firmicutes / Bacilli / Bacillales / Paenibacillaceae / Paenibacillus / 1 / 0.07 / 0 / 0
Unclassified / 2 / 0.15 / 0 / 0
Bacillaceae / Bacillus / 1 / 0.07 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Lactobacillales / Carnobacteriaceae / Dolosigranulum / 3 / 0.22 / 0 / 0
Streptococcaceae / Streptococcus / 1 / 0.07 / 0 / 0
Clostridia / Clostridiales / Unclassified / 1 / 0.07 / 0 / 0
Veillonellaceae / Unclassified / 1 / 0.07 / 0 / 0
Gemmatimonadetes / Gemmatimonadetes / Gemmatimonadales / Gemmatimonadaceae / Unclassified / 13 / 0.95 / 0 / 0
Planctomycetes / Planctomycetacia / Planctomycetales / Planctomycetaceae / Planctomyces / 7 / 0.51 / 0 / 0
Schlesneria / 1 / 0.07 / 0 / 0
Singulisphaera / 7 / 0.51 / 1 / 1.3
Unclassified / 22 / 1.61 / 5 / 6.3
Proteobacteria / Alphaproteobacteria / Rhizobiales / Beijerinckiaceae / Methylocella / 2 / 0.15 / 1 / 1.3
Chelatococcus / 3 / 0.22 / 0 / 0
Beijerinckia / 2 / 0.15 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Bradyrhizobiaceae / Bradyrhizobium / 18 / 1.31 / 0 / 0
Nitrobacter / 1 / 0.07 / 0 / 0
Rhodoblastus / 1 / 0.07 / 1 / 1.3
Blastobacter / 1 / 0.07 / 0 / 0
Afipia / 25 / 1.83 / 0 / 0
Unclassified / 2 / 0.15 / 0 / 0
Hyphomicrobiaceae / Pedomicrobium / 1 / 0.07 / 0 / 0
Hyphomicrobium / 1 / 0.07 / 0 / 0
Devosia / 1 / 0.07 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Methylocystaceae / Unclassified / 9 / 0.66 / 0 / 0
Xanthobacteraceae / Unclassified / 51 / 3.73 / 6 / 7.5
Unclassified / 47 / 3.43 / 0 / 0
Rhodospirillales / Acetobacteriaceae / Acidocella / 1 / 0.07 / 0 / 0
Acidisphaera / 1 / 0.07 / 0 / 0
Unclassified / 22 / 1.61 / 0 / 0
Unclassified / 134 / 9.79 / 4 / 5
Unclassified / 19 / 1.39 / 10 / 12.5
Betaproteobacteria / Burkholderiales / Burkholderiaceae / Burkholderia / 12 / 0.88 / 1 / 1.3
Alcaligenaceae / Unclassified / 1 / 0.07 / 0 / 0
Rhodocyclales / Rhodocyclaceae / Sterolibacterium / 0 / 0 / 1 / 1.3
Unclassified / 4 / 0.29 / 1 / 1.3
Nitrosomonadales / Nitrosomonadaceae / Unclassified / 1 / 0.07 / 0 / 0
Deltaproteobacteria / Myxococcales / Cystobacterineae / Unclassified / 3 / 0.22 / 0 / 0
Kofleriaceae / Haliangium / 0 / 0 / 1 / 1.3
Unclassified / 11 / 0.8 / 0 / 0
Unclassified / 26 / 1.9 / 3 / 3.8
Gammaproteobacteria / Legionellales / Coxiellaceae / Aquicella / 5 / 0.37 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Legionellaceae / Legionella / 2 / 0.15 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Chromatiales / Granulosicoccaceae / Granulosicoccus / 0 / 0 / 1 / 1.3
Xanthomonadales / Xanthomonadaceae / Dyella / 3 / 0.22 / 0 / 0
Rhodanobacter / 1 / 0.07 / 0 / 0
Dokdonella / 1 / 0.07 / 0 / 0
Unclassified / 4 / 0.29 / 0 / 0
Sinobacteraceae / Steroidobacter / 0 / 0 / 1 / 1.3
Unclassified / 41 / 2.99 / 0 / 0
Unclassified / 25 / 1.83 / 0 / 0
Unclassified / 15 / 1.1 / 0 / 0
Gemmatimonadetes / Gemmatimonadetes / Gemmatimonadales / Gemmatimonadaceae / Gemmatimonas / 7 / 0.51 / 0 / 0
Unclassified / 6 / 0.44 / 0 / 0
Verrucomicrobia / Opitutae / Opitutales / Opitutaceae / Opitutus / 6 / 0.44 / 0 / 0
Unclassified / 1 / 0.07 / 0 / 0
Spartobacteria / Candidatus_Xiphinematobacter / 11 / 0.8 / 2 / 2.5
Chthoniobacter / 3 / 0.22 / 0 / 0
Unclassified / 30 / 2.19 / 0 / 0
Unclassified / 23 / 1.68 / 2 / 2.5
Cyanobacteria / Unclassified / 14 / 1.02 / 0 / 0
Unclassified / 121 / 8.84 / 3 / 3.8
Total / 1369 / 100 / 80 / 100