Regulation and Function of Mecp2 Ser421 Phosphorylation in U50488-Induced Conditioned

Supplementary Material

Regulation and function of MeCP2 Ser421 phosphorylation in U50488-induced conditioned place aversion in mice

Anthony S. Zannas1,2,3, Jun H. Kim1 and Anne E. West1

1 Dept. of Neurobiology, Duke University Medical Center, Durham, NC USA 27710.

2 Dept. of Psychiatry and Behavioral Sciences, Duke University Medical Center, Durham, NC USA 27710.

3 Current address: Department of Translational Research in Psychiatry, Max Planck Institute of Psychiatry, Munich, Germany 80804.

Correspondence: Anthony S. Zannas () or Anne E. West ()

Table 1.Chamber times for C57BL/6 mice exposed to saline or 5mg/mg U50488

Chamber time in sec (mean±SEM) / Preference score (mean±SEM)
Center / "Drug"-Paired / Opposite
Saline, n=6 / Acclimation / 510±24 / 731±20 / 559±27 / 0.57 ± 0.02
Challenge / 449±40 / 693±43 / 656±64 / 0.52 ± 0.04
U50488, n=7 / Acclimation / 526±21 / 692±16 / 581±33 / 0.54±0.02
Challenge / 509±18 / 537±35 / 751±37 / 0.42 ± 0.03

Table 2.Chamber times for MeCP2 wild-type (WT) and Ser421 Ala knockin(KI) mice exposed to saline

Chamber time in sec (mean ± SEM) / Preference score (mean ± SEM)
Center / "Drug"-Paired / Opposite
WT, n=7 / Acclimation / 435±84 / 724±51 / 639±40 / 0.53 ± 0.01
Challenge / 448±46 / 645±79 / 706±37 / 0.47 ± 0.04
KI, n=6 / Acclimation / 479±52 / 634±45 / 686±52 / 0.47 ± 0.03
Challenge / 464±52 / 708±39 / 626±51 / 0.53 ± 0.03

Table 3.Chamber times for MeCP2 wild-type (WT)and Ser421Ala knockin(KI) mice exposed to 5mg/kg U50488

Chamber time in sec (mean ± SEM) / Preference score (mean ± SEM)
Center / "Drug"-Paired / Opposite
WT, n=13 / Acclimation / 430±25 / 724±28 / 645±23 / 0.53 ± 0.02
Challenge / 439±30 / 616±44 / 744±40 / 0.45± 0.03
KI, n=16 / Acclimation / 491±33 / 675±28 / 633±29 / 0.52± 0.02
Challenge / 471±20 / 623±39 / 704±32 / 0.47± 0.03