Product Insert Clostridium Screen K9760
Consists of 2 tubes, each tube yields 2 biochemical reactions. These 4 reactions can presumptively identify the major pathogens - Clostridium difficile and Clostridium perfringens from suitable cultures.
Tube 1 contains a TABLET and yields ONPG (beta galactosidase) and PYR (pyrridonyl aminopeptidase) results.
Tube 2 contains a DISC and yields PO4 (alkaline phosphotase) and PRO (proline aminopeptidase results.
TABLET ONPG & PYR
Add 5 drops of distilled water and inoculate heavily with a loop-full of organism from a fresh culture. Incubate aerobically uncovered for 2 hours at 370C. The appearance of a bright yellow colour is a positive ONPG result, a negative result is no change or a very pale yellow.
After recording the ONPG result add 1-2 drops of PEP reagent (spot indole). A colour change to pink or red or dark orange is a positive result for PYR. A negative result is yellow or pale orange.
DISC PO4 & PRO
Add 3 drops of distilled water and inoculate heavily with a loop-full of organism from a fresh culture. Incubate aerobically uncovered for 2 hours at 370C. The appearance of a bright yellow colour is a positive PO4 result, a negative result is no change or a very pale yellow.
After recording the PO4 result add 1-2 drops of PEP reagent (spot indole). A colour change to pink or red or dark orange is a positive result for PRO. A negative result is yellow or pale orange.
Results
ONPG / PYR (+PEP) / PO4 / PRO(+PEP)Positive / BRIGHT YELLOW / PINK/RED / BRIGHT YELLOW / PINK/RED
Negative / CLEAR/PALE YELLOW / YELLOW / CLEAR/PALE YELLOW / YELLOW
C. perfringens / positive / positive / positive / negative
C. difficile / negative / variable / negative / positive
C. glycolicum / positive
C. sordelli / negative / negative / negative / positive
C. bifermentans / negative / negative / negative / positive
Key Scientific