BioseparationEngineeringGroup
Processdevelopment,particletechnology,filtration,andextraction
nTheBioseparationEngineeringGroupdealswithdifferentaspectsoftheisolationandpurificationofbiomoleculesforthepharmaceuticalorchemicalindustry.Wearefocusedonadsorption,filtration,andextractionasseparationmethodsaswellastheirintegrationintotheproductionprocess.InadditiontotheexperimentalapproachesweuseCOMSOL
andCOSMO-RSformodellingandsimulation.
In2015,wecouldfinallyclaimthatbioseparationengineeringissuccessfullyestablishedasnewresearchfieldatTUMünchen.AsoneofthehighlightsthefirstPhDthesisinourgroupwasfinishedwiththefocusonantibodypurification.Thedevelopmentofnewhighselectivestation-aryphaseswithahighbindingcapacityyieldsanincreasedseparationproductivityofantibodiescomparedtocommercial
highlevelmatrices.
ProteinA–antibodycomplex.Copyright:Frhr.vonRoman,TUM
Prof.Dr.
SonjaBerensmeier
Contact
NewStationaryPhases
Newstationaryphasesareessentialinbioseparationsciences:classicalphasesarealreadywelldevelopedandreach
theirlimits.TheBioseparationEngineeringGroupisspecializedonsynthesisandfunctionalizationofmagneticparticles
andconductivematerialsaswellastheirprocessimplementation.Makinguseofmagnetismorconductivityallowsforanadditionaldegreeoffreedomforstate-of-
the-artprocessdevelopment.
Projects
nAiF/IFGProject–Synthesis,character-izationandapplicationofnewstationaryphasesforpotential-controlledchroma-tography
Optimizationofmagneticnanoparticlesynthesisbystatisticalapproaches.Copyright:Roth,TUM
BioseparationEngineeringGroup 87
FunctionalInterfaces
96wellscreeningplate
Inseparationsciencestheinteractionofparticulatecarriersamongeachotherandtotargetmoleculesisessentialforprocessdevelopment.Selectiveinteractionsas
wellashighbindingcapacitiesoftargetmoleculestosolidphasesdeterminethe
finalpurityandyieldandthereforethe
qualityinwholeoftheseparationstep.Incontrast,uncontrolledaggregationofparticlesdecreasesprocessperformanceconcerningrobustness,reproducibility,andscalability.Alltheseaspectsaremainfocustopicsofourprojects.
Projects
nBMBFBiotechnology2020+initiative
–rationaldesignofpeptide-surfaceinteractions
nAiF-ZIM–STEP-MAG:Verfahrenund
MessgerätzurQuantifizierungdermag-netischenMobilitätvonNanomaterialenundderenVerteilung
nDevelopmentofnewstationaryphases
forantibodypurification
ProcessDevelopment
Simulationofmagneticfields
Inadditiontotheoptimizationofclassicaldownstreamprocessesnewinnovativeseparationtechniquesaswellasintegratedprocessconceptsaresubjectsunder
research.Afocusareaistheresearchonhigh-gradientmagneticseparationandmembraneassistedextractionwithfocusonviscousmediawithhighlysolidcontent.
Projects
nBMBFProject–newenzymerecyclingstrategiesinindustrialprocesses
nBMBFe:Bioinitiative–SysBioTerp–
innovativestrategiesforasustainableproductionofbioactivemolecules
nVerfügungsfondMW–preparative
purificationofgastricporcinemucines
ResearchFocus
nDownstreamprocessing
nBioprocessintegration
nHigh-gradientmagneticseparation
nNewmagneticorconductiveparticles
nOptimizationofchromatographicprocesses
nBiomolecule-surface-interaction
nExtraction
Competence
nSynthesisandcharacterizationofnano-andmicroparticles
nSurfacefunctionalization
nMagneticseparationandautomation
nFermentation
nMolecularbiology,microbiology,biochemistry
nSimulationwithCOSMO-RS,COMSOL
Multiphysics,andSuperProDesigner
88 BioseparationEngineeringGroup
Infrastructure:
nS1–Labs(workingwithgeneticallymodifiedmicroorganisms–safetylevel1)
nParallelbioreactorsystem
nHigh-gradientmagneticseparator
(HGMS)
nDiversechromatography-andfiltration
systems
nParticle-/surfaceanalytics(DLS,BET,RAMAN,TGA-MS,contactangle,tensiometry)
nHPLCsystems
Courses
nBioseparationEngineeringI+II
nBiotechnologyforEngineers
nPracticalTrainingonBioprocess
Engineering
nPracticalTrainingonParticularNano-technology
nPracticalTrainingonPreparative
Chromatography
Management
Prof.Dr.SonjaBerensmeier,Director
AdministrativeStaff
Mrs.SusanneKuchenbaur,TeamAssistant
ResearchScientistsDr.PaulaFragaGarciaDr.ConstanzeFinger
Dr.MatthiasvonRomanSilviaBlank,M.Sc.MarkusBrammen,M.Sc.KerstinDieler,M.Sc.LjubomirGrozdev,M.Sc.LarsJanoschek,M.Sc.
Dipl.-Ing.Hans-ChristianRothVeronikaSchömig,M.Sc.SebastianSchwaminger,M.Sc.
TechnicalStaff
StefanDarchinger,BTA
Peer-reviewedPublications2015
nRothH-C,PramsA,LutzM,RitscherJ,RaabM,BerensmeierS:Ahigh-gradientmagneticseparatorforhighlyviscousprocessliquorsinindustrialbiotechnology,accepted,DOI:10.1002/ceat.201500398.
nMykhaylykO,LercheD,VlaskouD,SchoemigV,
DetloffT,KrauseD,WolffM,JoasT,BerensmeierS,PlankC:Magnetophoreticvelocitydeterminedbyspace-andtime-resolved-extinctionprofiles,IEEEMagneticsLetters,6,2015.
nSchwamingerSP,FragaGarcíaP,MerckGK,
BodensteinerFA,HeisslerS,GüntherS,Berens-meierS:Natureofinteractionsofaminoacidswithbaremagnetitenanoparticles,JPhysChemC,119,
23032-23041,2015.
nFragaGarcíaP,BrammenM,WolfM,Reinlein
S,FreiherrvonRomanM,BerensmeierS:
High-gradientmagneticseparationfortechnicalscaleproteinrecoveryusinglowcostmagneticnanoparticles,SepPurifTechnol,150,29-36,2015.
nHekmatD,MaslakD,FreiherrvonRomanM,
BreitschwerdtP,StröhleC,VogtA,BerensmeierS,Weuster-BotzD:Non-chromatographicpreparativepurificationofenhancedgreenfluorescentprotein,JBiotechnol,194,84-90,2015.
nRothH-C,SchwamingerSP,SchindlerM,Wagner
FE,BerensmeierS:Influencingfactorsinthe
co-precipitationprocessofsuperparamagneticironoxidenanoparticles:amodelbasedstudy,JMagnMagnMater,377,81–89,2015.
Reviews
nPeukerU,ThomasO,HobleyT,FranzrebM,
BerensmeierS,SchäferM,HicksteinB:Biosepara-tion,Magneticparticleadsorbents,bookchapter,EncyclopediaofIndustrialBiotechnology,JohnWileySons,2010.
nHeydM,KohnertA,TanT-H,NusserM,Kirschhöfer
F,Brenner-WeissG,FranzrebM,BerensmeierS:Developmentandtrendsofbiosurfactansanalysisandpurificationusingrhamnolipidsasexample,review,Anal.Bioanal.Chem.391(5),1579-1590,
2008.
nBerensmeierS:Magneticparticlesforseparationandpurificationofnucleicacidmolecules,review,Appl.Microbiol.Biotechnol.73,495-504,2006.
BioseparationEngineeringGroup 89