APPENDIX 7

OTHER EXPERIMENTAL PROCEDURES

REMINDER: FOR EINSTEIN’S BEHAVIORAL CORE YOU SHOULD COMPLETE THIS APPENDIX AND YOU WILL BE REQUIRED TO ATTACH AN EMAIL FROM THE CORE DIRECTOR(SEE ITEM 15 IN MAIN PROTOCOL FORM)
  1. Describe any and all procedures not covered in other appendices. Check below

Non -noxious Testing / Potentially Stressful / Noxious Testing
☐ Positive reward conditioning / ☐ Morris water maze / ☐Stress induction (cold, footshock, restraint) (radiography, MRI)
☐ Open field behavior / ☐ Addiction / drug withdrawal / ☐ Negative conditioning (footshock)
☐ Imaging procedures / ☐ Tail Cuff Blood Pressure Measurement / ☐ Tail flick test
☐ Electrophysiology (ECG, EEG, EMG) / ☐ Seizure induction
☐ Metabolism Cage / ☐ Restraint device – Prolong > 30 minutes / ☐Irradiation
☐ MRI / ☐ Microdialysis
☐ Radiography
☐ MicroPET
If something other than those items listed above, please describe below.
☐ Other.
Describe:
  1. Indicate location where procedure will be performed.

Respond below.
  1. Provide frequency of test sessions.

☐Every day. / ☐Every two days.
☐Every other day. / ☐Other. Indicate frequency below.
Frequency:
  1. Provide duration of each test session.

Respond below.
  1. Indicate maximum number of test sessions on each animal.

Respond below.
  1. Total duration of other experimental procedures.

☐ Hours: / ☐ Days / ☐ Weeks
☐ Months / ☐ Other (indicate other):
  1. Describe the parameters of each procedure and expected outcome. (Test parameters include the stimulus, stimulus intensity, stimulus duration). Include the “readouts”, the physical or behavior modifying characteristics of the stimulus or material administered or withdrawn.

Respond below.
  1. Will any procedure cause more than momentary pain or discomfort?

☐No. / ☐Yes. Complete items A and B below.

8A. Describe below the procedures or methods used to minimize pain and discomfort or explain why such methods cannot be used. You must include this search for alternatives in item 21 of main protocol form.

Respond below.

8B. Define specific humane criteria for removal of an animal from a potentially painful or stressful procedure such as failure to succeed in a specified time, signs of exhaustion, inappropriate reactions, etc.

Respond below.
  1. Describe the methods for monitoring the condition of the animal during and after the procedure.

Respond below.
  1. Provide the names and phone numbers of the person(s) who will perform these procedures and are responsible for monitoring the condition of the animals.

Name / Work Phone / After hours phone, pager or beeper #
  1. Are you obtaining services from Einstein’s Behavioral Core?

☐No. / ☐Yes. See below.
If “Yes” is checked above, you MUST attach email from Core Director confirming that Core Director is aware of services being requested in this protocol. You can obtain the email from the Behavioral Core.

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