NO:0947/LP/ Equipment/20/ASAB

NUST (ASAB)

NATIONAL UNIVERSITY OF SCIENCES& TECHNOLOGY

TENDER NOTICE

LAB EQUIPMENT

Atta-ur-Rahman School of Applied Biosciences (ASAB), constituent school of NUST invites sealed Tenders from original manufacture / authorized dealersetc holding NTN and sales tax registration for the purchase of following Lab Equipment from renowned USA, UK, Germany and Europe based companies or equivalent:

S. No. / Item Name / Quantity Required
1. / Biolistic Gun (Gene Gun) / 01 No
2. / Lypholiser (Freeze Dryer Machine) / 01 No
3. / French Press (Cell Disruption Machine) / 01 No
4. / Incubator Shaker (Refrigerated)
(Auto restart after power failure) / 01 No
5. / Drying Incubator (2200C) / 01 No
6. / Gradient Thermal Cycler
(Auto restart after power failure. Lid temperature control feature, should accommodate tubes, strips/ plates.) / 02 No`s

Tender Documents containing the terms & conditions along with detailed specifications of items can be obtained from under mentioned address from 0930 hrs to 1600 hrs latest by 09th April 2018 at a cost of Rs. 1000/- (non-refundable) in the form of a Cash/cheque/bank draft payable to ASAB-NUST Islamabad.Bids are required on both FOR and CPT Basis.

The quotations/bids at addressed to President LPC, ASAB and should reach his office by 26th April 2018 at 1400 hrs, which would open in the presence of the representative of the firms at 1500hrs on Same day. Competent authority at NUST has reserve the right to change in quantity or cancel the tender at any stage without any liability

President LPC, ASAB

Atta-ur-Rahman School of Applied Biosciences (ASAB)

National University of Sciences and Technology

H-12 NUST Campus Islamabad

Phone: 051-90856121, Fax: 051-90856102

Email:

SPECIFICATIONS

  1. Gene Gun
  2. PDS-1000/He Hepta System,
  3. Includes PDS-1000/He System, Hepta adaptor, Helium pressure regulator, solenoid, spacer rods, micro carriers launch assembly, target shelf, 5 Micro carriers holders, tubing and instruction.
  4. Helium Gas cylinder grade 4.5 (99.995% pure). Pressurized to 2600 PSI vacuum source.
  5. Construction: Aluminum, ABS Plastic and Acrylic chassis.
  6. Dimension: 29 x 25.5 x 47.5 cm
  7. Weight: 15 Kg
  8. Max Current: < 5Amp
  9. Vacuum: < 0.4 inches Mercury/Minute Leakage
  1. Pressure: 450 - 2400 PSI
  2. High reproducible Transfection with Hepta Adaptor. Transfection Area 7-10 times more cells than standard system. Doubling the biolistic target area from 40 cm² to 75 cm².
  3. Pressure regulator for Helium cylinder (with in-line 0.45 u filter).
  4. Licenses Product, Biolistic Technology
  5. Nine interchangeable disks 450,650,900,1100,1350,1550,1800,2000 and 2200
  6. Used with Gold Micro particles available in range 0.6, 1.0 and 1.6 um.
  7. Particle Delivery Systems Training and Application guide CD-ROM.
  8. Voltage Converter PDS-1000/He System
  9. 500 Optimization Kit, includes 0.25 each of 0.6 um and 1.6 um gold micro carriers, 100 each of 9 rupture disks, 500 Micro carriers, 500 stopping screens.
  10. Vacuum Pump compatible with Gene Gun (may be locally provided)

Origin: USA, UK, Germany or Equivalent

  1. Lypholiser (Freeze Dryer Machine)
  2. Freeze Dryer, Bench top Cascade (dual refrigeration) Stoppering Tray Dryer, one large shelf and four valves, 230V, 50Hz
  3. Accessories:Vacuum Pump with filter exhaust 220V/50 Hz
  4. Tray with Slide-out Bottom for loading glass vials in shelves

Origin: USA, UK, Europe or Equivalent

  1. French Press (Cell Disruption Machine)

Press

  1. Height 34.5"(87.6 cm)
  2. Dimensions 18.25 x 18.25" (46.4 x 46.4 cm)
  3. Power Required 115 V, 60 Hz or 220 V, 50 Hz

Cell

  1. Diameter 3" (7.6 cm)
  2. Closed Height 11.38" (28.9 cm)
  3. Open Height 14.25" (36.5 cm)
  4. Sample Capacity 35 ml max
  5. Minimum Volume First Run 1.2 ml and Additional Runs 0.7 ml
  6. Maximum Working Pressure 40,000 psi
  7. Bore Size 1" (2.5 cm)

Make: USA, UK, Europeor Equivalent

  1. Incubator Shaker (Refrigerated)
  2. Large stackable incubated and refrigerated floor shaker.
  3. Speed Range: 15-500 ±1 Rpm.
  4. Temperature Range: 15°C below ambient to 80°C.
  5. With 18” x 18” (45.7 x 45.7 cm) Platform and Clamp Starter Kit (Includes : (2) 125 ml flask clamps, (4) 250 ml flask clamps, 2) 1L flask clamps & (2) 2L flask clamps)
  6. Speed Range: 15-500 ±1 Rpm.
  7. Orbit Shaking Diameter: 0.75 (1.9) cm.
  8. Temperature Range & Accuracy: 15°C below ambient to 80°C, ±0.1°C at 37°C in flask.
  9. Overall Dimensions (L x W x H) : 33 x 27 x 40.5 Inches.
  1. Maximum Load: 430 lbs : 195 kg.
  2. Power: 240V, 50/60 Hz.
    i.Displayed time counts up during continuous operation and down during timed runs.
    ii. View speed, operating time and temperature simultaneously on three individual LED displays which helps minimize errors in establishing running conditions.
    iii. Visual/audible signals alert you to temperature deviations. Heat turns off if temperature deviates ±1°C of set point.
    iv.Soft start feature eliminates splashing of vessel contents, wetting of flask closure, and helps prevent sudden starts and stops.
    v.Set point retention retains parameters during power failure and restarts unit automatically after power is restored.

Origin: USA, UK, Germanyor Equivalent

  1. Drying Incubator (2200C)
  2. Internal volume between 100-120 liters
  3. Stainless steel perforated shelves with loading capacity up to 18 kg each
  4. Loading capacity of chamber up to 80 kg
  5. Must have standard sterilization program
  6. Stainless steel water dish as accessory
  7. Insulated stainless steel door
  8. Working temperature range 5 above ambient temperature up to +2200C
  1. Gradient Thermal Cycler
  2. The system should be able to perform Gene expression, Plus/Minus assay, SNP, allelic discrimination and dissociation curve analysis etc.
  3. A 96 well (48x2) thermal cycler with temperature range of 4oC to 100oC.
  4. Reaction volume 10-40 µl.
  5. Should be compatible to standard 96 well (48x2), 8 well strips, or individual PCR tubes.
  6. All assays should run using universal thermal cycling conditions to eliminate optimization of PCR conditions.
  7. The excitation by Tungsten Halogen/Xenon source and detection by cooled CCD camera or LED based excitation.
  8. There should be enough excitation and emission filters to cover majority of dyes.
  9. The instrument should be pre-calibrated for at least seven dyes including the following during installation at the customer site: FAMTM/SYBR® Green I, VIC®/JOETM, NEDTM/TAMRA TM/ and ROX TM. The user should be able to use any of these dyes in an experiment without needing to recalibrate the instrument. Addition of new dyes should be possible without hardware change.
  1. System should be standardized for at least two homogeneous reaction chemistries including SYBR Green I and dual color TaqMan or four color hybridization probes (FRET).
  2. The instrument software must be capable of detecting and analyzing a different gene, SNP or pathogen target in every well of the plates.
  3. Dedicated licensed full version software for primer and probe design with comprehensive assay design and development guidelines for quantitative and qualitative real-time assays should be provided to enable designing of custom oligo assays.
  4. Analysis work station should be of latest configuration with a color printer
  5. Compatible online UPS (2KV)