Comparative hepatotoxicity of 6:2 fluorotelomer carboxylic acid and 6:2 fluorotelomer sulfonic acid, two fluorinated alternatives, on adult male mice

Nan Sheng†, Xiujuan Zhou†, Fei Zheng₴, Yitao Pan†, Xuejiang Guo₤ and Jiayin Dai†a

†Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, P.R. China.

₴Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi, 030801, P.R. China;

₤State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, P.R. China

Supporting Information Available

Table S1 Primer sequences used for real-time PCR amplification

Table S2 Twelve significantly changed genes in the livers of the 6:2 FTCA and 6:2 FTSA exposure groups

Fig. S1 Body weights before and after treatment. Differences were evaluated using One-way ANOVA

Fig. S2 Possible genes related to apoptosis and necrosis influenced by 6:2 FTSA exposure


Table S1 Primer sequences used for real-time PCR amplification

Gene name / Accession No. / Description / Sequence (5’-3’) / Length (bp)
18S / NR_003278.3 / Forward / GCCGTTCTTAGTTGGTGGA / 159
Reverse / GACCTGTTATTGCTCAATCTCG
CD36 / NM_001159558.1 / Forward / CACTGGCTACATCTTTGG / 101
Reverse / CACCACCATTTCTTCTCC
PPARα / NM_001113418.1 / Forward / AGAGGTCCGATTCTTCCA / 310
Reverse / ATCCAGTTCTAAGGCATTGA
Acox1 / NM_015729.2 / Forward / TAACTTCCTCACTCGAAGCCA / 283
Reverse / AGTTCCATGACCCATCTCTGTC
FABP1 / NM_017399.4 / Forward / ATGAACTTCTCCGGCAACTACC / 118
Reverse / CTGACACCCCCTTGATGTCC
Cyp4a10 / NM_010011.3 / Forward / CCCAAGTGCCTTTCCTA / 149
Reverse / GCAAACCATACCCAAATCC
Cpt1a / NM_013495.2 / Forward / TGGCATCATCACTGGTGTGTT / 133
Reverse / GTCTAGGGTCCGATTGATCTTTG
PPARγ / NM_001127330.1 / Forward / GGAAGACCACTCGCATTCCTT / 121
Reverse / GTAATCAGCAACCATTGGGTCA
Srebf-1 / NM_011480.3 / Forward / CAAGGCCATCGACTACATCCG / 154
Reverse / GCCCTCCATAGACACATCTGT
Srebf-2 / NM_033218.1 / Forward / GTGGGAGAGTTCCCTGATTTG / 112
Reverse / CTCCACCATTGTTGCCTCTG
HNF4α / NM_008261.2 / Forward / AAGGTGCCAACCTCAATTCATC / 177
Reverse / CACATTGTCGGCTAAACCTGC
CAR / NM_009803.5 / Forward / GTCCCATCTGTCCGTTTG / 117
Reverse / TGTCTTTCCTCATGCCAAC
HMGCR / NM_008255.2 / Forward / GATTCTGGCAGTCAGTGGGAA / 214
Reverse / GTTGTAGCCGCCTATGCTCC
Gene / up/down / Associated gene name / Description
ENSMUSG00000025936 / down / Gm4956 / Predicted gene 4956
ENSMUSG00000072324 / down / Gm8420 / Predicted gene 8420
ENSMUSG00000015224 / down / Cyp2j9 / Cytochrome P450, family 2, subfamily j, polypeptide 9
ENSMUSG00000059751 / down / Rps3a3 / Ribosomal protein S3A3
ENSMUSG00000042638 / up / Gucy2c / Guanylate cyclase 2c
ENSMUSG00000063838 / up / Cdc42ep5 / CDC42 effector protein (Rho GTPase binding) 5
ENSMUSG00000031765 / up / Mt1 / Metallothionein 1
ENSMUSG00000027014 / up / Cwc22 / CWC22 spliceosome-associated protein homolog (S. cerevisiae)
ENSMUSG00000044534 / up / Ackr2 / Atypical chemokine receptor 2
ENSMUSG00000026822 / up / Lcn2 / Lipocalin 2
ENSMUSG00000048388 / up / Fam171b / Family with sequence similarity 171, member B
ENSMUSG00000030483 / up / Cyp2b10 / Cytochrome P450, family 2, subfamily b, polypeptide 10

Table S2 Twelve significantly changed genes in the livers of the 6:2 FTCA and 6:2 FTSA exposure groups

Fig. S1 Body weights before and after treatment. Differences were evaluated using two-tailed t-tests

Fig. S2 Possible genes related to apoptosis and necrosis influenced by 6:2 FTSA exposure