Kawaji et al. Suppl. Fig. 1B
(MMU)D6Wsu147e 1 -----MAAGMYLEHYLDS------IENLPFELQRNFQLMRDLDQ
(MMU)D6Wsu147e [var.] 1 -----MAAGMYLEHYLDS------IENLPFELQRNFQLMRDLDQ
(HSA)LOC51147 1 -----MAAGMYLEHYLDS------IENLPFELQRNFQLMRDLDQ
(HSA)MY036 1 -----MAAGMYLEHYLDS------IENLPFELQRNFQLMRDLDQ
(MMU)1700027H23Rik 1 ------MRELDQ
(MMU)1810018M11Rik 1 -----MATAMYLEHYLDS------IENLPCELQRNFQLMRELDQ
(DMEL)CG9293 1 -----MSSAIYLENYLDGKKILKHGKHSKTYIIHD-LQTGLESLPTELERNFKLMRKLDD
(ATHA)F14013 1 -----MSFAEEFEANLVS------LAHVLQKKYALLRDLDK
(ATHA)F20D21.22 1 ------MFVFCLSLEALINQTRQ
(SPOM)C3G9.08 1 ------MADDT-AYILSEYLQT-LDNVPNETKHIFDEISVKEV
consensus/70% Mu.u..hEt.Lsu l-sLPhELp+pFpLMR-LDQ
TxK
SxR ### #####
(MMU)D6Wsu147e 34 RTEDLKAEIDKLATEYMSSAR---SLSSEEKLALLRQIQEAYGKCKEFG---DDKVQLAM
(MMU)D6Wsu147e [var.] 34 RTEDLKAEIDKLATEYMSSAR---SLSSEEKLALLRQIQEAYGKCKEFG---DDKVQLAM
(HSA)LOC51147 34 RTEDLKAEIDKLATEYMSSAR---SLSSEEKLALLKQIQEAYGKCKEFG---DDKVQLAM
(HSA)MY036 34 RTEDLKAEIDKLATEYMSSAR---SLSSEEKLALLKQIQEAYGKCKEFG---DDKVQLAM
(MMU)1700027H23Rik 7 RTEDKKAEIDILAAEYISTVK---TLSSAQRVEHLQKIQSAYSKCKEYS---DDKVQLAM
(MMU)1810018M11Rik 34 RTEDKKAEIDILAAEYISTVK---TLSSAQRVEHLQKIQSAYSKCKEYS---DDKVQLAM
(DMEL)CG9293 55 RAQTAMKSIDSHAKDFMRKLGENGAMSEDERRERQEDIKALFGKAKEYS---DDKVQLAI
(ATHA)F14013 31 SLQENQRQNEQRCEKEIEDIRRGRAGNITPNTSLTKFSEEALDEQKHSVRIADEKVTLAM
(ATHA)F20D21.22 18 QTKYCLGLASQSSKKGNGNHYN--NGGLDEEETIEKMRKEIESSQENALSLCTEKVLLAR
(SPOM)C3G9.08 36 AVHDIWKRIQAADSQIQSYIKS--HGSLTPHPKEDALYSTIREEYQKAINIQNEKVQLAD
consensus/70% RTcDhpucIDphAs-ahSsh+. shSppp+hthhcpIppAauKsKEau DDKVQLAM
+++++++++++++MDS00105.2++++
KxxExxx Y
(MMU)D6Wsu147e 88 QTYEMVDKHIRRLDTDLARFEADLKEKQIESSD------YDS
(MMU)D6Wsu147e [var.] 88 QTYEMVDKHIRRLDTDLARFEADLKEKQIESSD------YDS
(HSA)LOC51147 88 QTYEMVDKHIRRLDTDLARFEADLKEKQIESSD------YDS
(HSA)MY036 88 QTYEMVDKHIRRLDTDLARFEADLKEKQIESSD------YDS
(MMU)1700027H23Rik 61 QTYEMVDKHIRRLDADLARFEADLKDR-MDGSD------FES
(MMU)1810018M11Rik 88 QTYEMVDKHIRRLDADLARFEADLKDR-MDGSD------FES
(DMEL)CG9293 112 QTYELVDKQIRRLDNDLARFEGEIQEKASSTRAKSEEVVAKSKTVPKYLFFHKLTPSFDS
(ATHA)F14013 91 QAYDLVDMHVQQLDQYMKKSDEVIRKEKEAAAATLELENNG------
(ATHA)F20D21.22 76 QAYDLIDSHVKRLDEDLNNFAEDLKQEGKIPPDE-PSVLPPLPIVPKA-SGA------
(SPOM)C3G9.08 94 RARLGLTRHIKRLDDRLAKAGHGFTAAELLNAPDYYASSPYGGYSPSGASSARQTPAPSR
consensus/70% QTYEhVDKHIRRLDsDLARFEuDLK-+thpuuD...... a-S
+++++MDS00105.2+++++++++++++
SxKSxK TxK SxR SxK
(MMU)D6Wsu147e 124 SSSKGKKS-----RTQKEKKAARARSK------GKNSDE
(MMU)D6Wsu147e [var.] 124 SSSKGKKS-----RTQKEKKAARARSK------GKNSDE
(HSA)LOC51147 124 SSSKGKKKG----RTQKEKKAARARSK------GKNSDE
(HSA)MY036 124 SSSKGKKS-----RTQKEKKAARGRSK------GKNSDE
(MMU)1700027H23Rik 96 TGARSLKKG----RSQKEKRSSRGRGR------RTSEE
(MMU)1810018M11Rik 123 TGARSLKKG----RSQKEKRSSRGRGR------RTSEE
(DMEL)CG9293 172 ICTEGRKKTKDSKTTGKKKKSASSDEETGRGNNQSNANSSVNSSSNAGQGSKKKKSKVNQ
(ATHA)F14013 132 ------KAGNAGEGGRGG------RKKTRLAT-
(ATHA)F20D21.22 126 ------EKR------KSFYGTPQ
(SPOM)C3G9.08 154 ------STAGRRRTS-ATTRG------AIQNGVYHSPYTAS
consensus/70% .sscuhKp. potKEK+uucucs+ ..t+sosp
*** **************
TxK SxK
(MMU)D6Wsu147e 152 EAPKAAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(MMU)D6Wsu147e [var.] 152 EAPKAAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(HSA)LOC51147 153 EAPKTAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(HSA)MY036 152 EAPKTAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(MMU)1700027H23Rik 124 D--TPKKKKHK----SGSEFTDSI--LSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(MMU)1810018M11Rik 151 D--TPKKKKHK----SGSEFTDSI--LSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGC
(DMEL)CG9293 232 EKETRKGGAQKKTVEVDDSEKESCHTAATHPSDVMDMPVDPNEPTYCLCHQVSYGEMIGC
(ATHA)F14013 152 AASTA------AASTGMTSSNMDLDLPVDPNEPTYCICNQVSFGEMVACD
(ATHA)F20D21.22 137 -PKKIDYRDRDWDRDRDFELMPPPGSNRKDLMPIEEQPIDPNEPTYCVCHQVSFGDMIAC
(SPOM)C3G9.08 182 LADSGSTRGQKVSNATATTQLETKADSTTPNEMVSEEDMEEDNEKYCFCQQGSYGQMVAC
consensus/70% -s.pstp+chKhs.tosp.hhsSss.sosHPSDVhDMPVDPNEPTYCLCHQVSYGEMIGC
+++++C+C++++++++++C
PHD Finger
T xK
(MMU)D6Wsu147e 212 DNPDCS-IEWFHFACVGLT--TKPRGKWFCPRCSQERKKK------
(MMU)D6Wsu147e [var.] 212 DNPDCS-IEWFHFASCGADNQPRGKWX-F-PRCSQERRKIVPGSITDFCIPLTWASGQSG
(HSA)LOC51147 213 DNPDCS-IEWFHFACVGLT--TKPRGKWFCPRCSQERKKK------
(HSA)MY036 212 DNPDCS-IEWFHFACVGLT--TKPRGKWFCHAAPRTEEEIDKGLGFQHSFFHIP------
(MMU)1700027H23Rik 176 DNPDCP-IEWFHFACVDLT--TKPKGKWFCPRCVQEKRKKK------
(MMU)1810018M11Rik 203 DNPDCP-IEWFHFACVDLT--TKPKGKWFCPRCVQEKRKKK------
(DMEL)CG9293 292 DNPDCP-IEWFHFACVGLT--TKPKGKWFCPKCTQDRKKK------
(ATHA)F14013 196 NNACK-IEWFHFGCVGLK--EQPKGKWYCPECATVKKSRKGR------
(ATHA)F20D21.22 196 DNENCQGGEWFHYTCVGLTPETRFKGKWYCPTCRLLPQSH------
(SPOM)C3G9.08 242 DNANCE-REWFHMECVGLK--APPEGTWYCEACRDQKLVDAK------
consensus/70% DNPDCs IEWFHFACVGLT T+P+GKWFCPpCspc+c++..
++++C+++++++++C++++++++++++++C++C++
PHD Finger
(MMU)D6Wsu147e ------
(MMU)D6Wsu147e [var.] 269 SLCWGRGPREMWTVRYRHPSPSSPLGAEAASKXPVGRVLQPVTVCALLPPLPEGSNLTHC
(HSA)LOC51147 ------
(HSA)MY036 ------
(MMU)1700027H23Rik ------
(MMU)1810018M11Rik ------
(DMEL)CG9293 ------
(ATHA)F14013 ------
(ATHA)F20D21.22 ------
(SPOM)C3G9.08 ------
consensus/70%
(MMU)D6Wsu147e ------
(MMU)D6Wsu147e [var.] 329 PFASLVRLVLYFRGRVLFIPLAL
(HSA)LOC51147 ------
(HSA)MY036 ------
(MMU)1700027H23Rik ------
(MMU)1810018M11Rik ------
(DMEL)CG9293 ------
(ATHA)F14013 ------
(ATHA)F20D21.22 ------
(SPOM)C3G9.08 ------
consensus/70%
Edited ClustalW and MView post-processed alignment of representative ING1-homolog subfamily members. The positions of motif MDS00105.2 and the PHD finger are indicated by “+”. The ING1 homolog submotif positions are designated by “#”. Predicted protein tyrosine kinase ([RK]-x2-3[DE]-x2-3-Y) and protein kinase C phosphorylation ([ST]-x-[RK]) sites are indicated. The positions of PSORT predicted bipartite nuclear localization signals are shown by asterisk (except (SPOM)C3G9.09, (ATHA) F14013 and (ATHA)F20D21.22.) Classical nuclear localization signals (not shown) are present in all sequences except in (SPOM)C3G9.09, (ATHA) F14013 and (ATHA)F20D21.22. The SWISS-PROT/TrEMBL accessions of the sequences are: (MMU)D6Wsu147e, Q9D7F9; (HSA)LOC51147, Q9UNL4; (HSA)MY036, Q9H3J0; (DMEL)CG9293, Q9VJY8; (ATHA)F14013, Q9LIQ6; (ATHA)F20D21.22, Q9SLJ4; and (SPOM)C3G9.08, O42871. (MMU)D6Wsu147e [variant] is the putative translation of DDBJ entry AK002821 (FANTOM clone ID 0610039A16). The species abbreviations are: HSA, Homo sapiens; MMU, Mus musculus; DMEL, Drosophila melanogaster; ATHA, Arabidopsis thaliana; SPOM, Schizosaccharomyces pombe.