LOCUS Exported File 2788 Bp Ds-DNA Circular SYN 18-APR-2013
LOCUS Exported File 2788 bp ds-DNA circular SYN 18-APR-2013
DEFINITION Entry vector for transferring a cloned attL-flanked gene to a
destination vector in the Gateway(R) system.
ACCESSION .
VERSION .
KEYWORDS pENTR223 with ORF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2788)
AUTHORS I.M.A.G.E Consortium
TITLE Direct Submission
JOURNAL Exported Thursday, Apr 18, 2013 from SnapGene 1.5.2
FEATURES Location/Qualifiers
source 1..2788
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
terminator 387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
primer_bind 537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
protein_bind 569..662
/bound_moiety="LR Clonase(TM)"
/note="attL1'"
/note="recombination site for the Gateway(R) LR reaction;
mutated version of attL1"
/note="color: #31849b"
gap 663..672
/estimated_length="10"
/note="color: #------"
misc_feature 663..672
/note="ORF insertion site"
/note="color: #a6acb3"
protein_bind 673..766
/bound_moiety="LR Clonase(TM)"
/note="attL2'"
/note="recombination site for the Gateway(R) LR reaction;
mutated version of attL2"
/note="color: #31849b"
promoter complement(784..802)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind complement(807..823)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
CDS 1254..2045
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/note="color: #ccffcc"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 2138..2726
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga
61 taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga
121 gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggca
181 cgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatacgcgtaccgc
241 tagccaggaagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgctta
301 gtttgatgcctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttc
361 acaacgttcaaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaa
421 caacagataaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctg
481 gcagttccctactctcgcgttaacgctagcatggatgttttcccagtcacgacgttgtaa
541 aacgacggccagtcttaagctcgggccccaaataatgattttattttgactgatagtgac
601 ctgttcgttgcaacaaattgatgagcaatgcttttttataatgccaactttgtacaaaaa
661 agnnnnnnnnnnctttcttgtacaaagttggcattataagaaagcattgcttatcaattt
721 gttgcaacgaacaggtcactatcagtcaaaataaaatcattatttgccatccagctgata
781 tcccctatagtgagtcgtattacatggtcatagctgtttcctggcagctctggcccgtgt
841 ctcaaaatctctgatgttacattgcacaagataaaaatatatcatcatgcctcctctaga
901 ccagccaggacagaaatgcctcgacttcgctgctacccaaggttgccgggtgacgcacac
961 cgtggaaacggatgaaggcacgaacccagtggacataagcctgttcggttcgtaagctgt
1021 aatgcaagtagcgtatgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtg
1081 gtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtcta
1141 tgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatgga
1201 gcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgaggg
1261 aagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgcc
1321 atctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctga
1381 agccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgc
1441 ggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattc
1501 tccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccag
1561 ctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcg
1621 agccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcg
1681 ttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctat
1741 ttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatg
1801 agcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcg
1861 cgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccg
1921 tcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcg
1981 cagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggca
2041 aataaccctcgagccacccatgaccaaaatcccttaacgtgagttacgcgtcgttccact
2101 gagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcg
2161 taatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatc
2221 aagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaata
2281 ctgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgccta
2341 catacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtc
2401 ttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacgg
2461 ggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctac
2521 agcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccgg
2581 taagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt
2641 atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgct
2701 cgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctgg
2761 ccttttgctggccttttgctcacatgtt
//