LOCUS Ago2-S387A-Flag- 8004 bp DNA
FEATURES Location/Qualifiers
CDS complement(7008..7868)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 3604..3828
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(3598..3615)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(7869..7967)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 3874..4302
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
CDS 4712..5506
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(6193..6863)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 4307..4676
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 5680..5810
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
CDS 929..3553
/vntifkey="4"
/label=Ago2-S387A-Flag
misc_feature 935..958
/vntifkey="21"
/label=Flag
BASE COUNT 1881 a 2164 c 2089 g 1870 t
ORIGIN
1 gacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatg
61 ccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcg
121 cgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgc
181 ttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacatt
241 gattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatata
301 tggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacc
361 cccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttcc
421 attgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgt
481 atcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcatt
541 atgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtca
601 tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttg
661 actcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcacc
721 aaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcg
781 gtaggcgtgtacggtgggaggtctatataagcagagctctctggctaactagagaaccca
841 ctgcttactggcttatcgaaattaatacgactcactatagggagacccaagctggctagc
901 gtttaaacttaagcttggtaccgagctcaccatggactacaaagacgatgacgacaaggg
961 atccgaattcggtgttctctctgccattcccgcacttgcacctcctgcgccgccgccccc
1021 catccaaggatatgccttcaagcctccacctagacccgactttgggacctccgggagaac
1081 aatcaaattacaggccaatttcttcgaaatggacatccccaaaattgacatctatcatta
1141 tgaattggatatcaagccagagaagtgcccgaggagagttaacagggaaatcgtggaaca
1201 catggtccagcactttaaaacacagatctttggggatcggaagcccgtgtttgacggcag
1261 gaagaatctatacacagccatgccccttccgattgggagggacaaggtggagctggaggt
1321 cacgctgccaggagaaggcaaggatcgcatcttcaaggtgtccatcaagtgggtgtcctg
1381 cgtgagcttgcaggcgttacacgatgcactttcagggcggctgcccagcgtcccttttga
1441 gacgatccaggccctggacgtggtcatgaggcacttgccatccatgaggtacacccccgt
1501 gggccgctccttcttcaccgcgtccgaaggctgctctaaccctcttggcgggggccgaga
1561 agtgtggtttggcttccatcagtccgtccggccttctctctggaaaatgatgctgaatat
1621 tgatgtgtcagcaacagcgttttacaaggcacagccagtaatcgagtttgtttgtgaagt
1681 tttggattttaaaagtattgaagaacaacaaaaacctctgacagattcccaaagggtaaa
1741 gtttaccaaagaaattaaaggtctaaaggtggagataacgcactgtgggcagatgaagag
1801 gaagtaccgtgtctgcaatgtgacccggcggcccgccagtcaccaaacattcccgctgca
1861 gcaggagagcgggcagacggtggagtgcacggtggcccagtatttcaaggacaggcacaa
1921 gttggttctgcgctacccccacctcccatgtttacaagtcggacaggagcagaaacacac
1981 ctaccttcccctggaggtctgtaacattgtggcaggacaaagatgtattaaaaaattaac
2041 ggacaatcagacctcaaccatgatcagagcgactgctaggtcggcgcccgatcggcaaga
2101 agagattagcaaattgatgcgaagtgcagccttcaacacagatccatacgtccgtgaatt
2161 tggaatcatggtcaaagatgagatgacagacgtgactgggcgggtgctgcagccgccctc
2221 catcctctacgggggcaggaataaagctattgcgacccctgtccagggcgtctgggacat
2281 gcggaacaagcagttccacacgggcatcgagatcaaggtgtgggccattgcgtgcttcgc
2341 cccccagcgccagtgcacggaagtccatctgaagtccttcacagagcagctcagaaagat
2401 ctcgagagacgccggcatgcccatccagggccagccgtgcttctgcaaatacgcgcaggg
2461 ggcggacagcgtggagcccatgttccggcacctgaagaacacgtatgcgggcctgcagct
2521 ggtggtggtcatcctgcccggcaagacgcccgtgtacgccgaggtcaagcgcgtgggaga
2581 cacggtgctggggatggccacgcagtgcgtgcagatgaagaacgtgcagaggaccacgcc
2641 acagaccctgtccaacctctgcctgaagatcaacgtcaagctgggaggcgtgaacaacat
2701 cctgctgccccagggcaggccgccggtgttccagcagcccgtcatctttctgggagcaga
2761 cgtcactcacccccccgccggggatgggaagaagccctccattgccgccgtggtgggcag
2821 catggacgcccaccccaatcgctactgcgccaccgtgcgcgtgcagcagcaccggcagga
2881 gatcatacaagacctggccgccatggtccgcgagctcctcatccagttctacaagtccac
2941 gcgcttcaagcccacccgcatcatcttctaccgcgacggtgtctctgaaggccagttcca
3001 gcaggttctccaccacgagttgctggccatccgtgaggcctgtatcaagctagaaaaaga
3061 ctaccagcccgggatcaccttcatcgtggtgcagaagaggcaccacacccggctcttctg
3121 cactgacaagaacgagcgggttgggaaaagtggaaacattccagcaggcacgactgtgga
3181 cacgaaaatcacccaccccaccgagttcgacttctacctgtgtagtcacgctggcatcca
3241 ggggacaagcaggccttcgcactatcacgtcctctgggacgacaatcgtttctcctctga
3301 tgagctgcagatcctaacctaccagctgtgtcacacctacgtgcgctgcacacgctccgt
3361 gtccatcccagcgccagcatactacgctcacctggtggccttccgggccaggtaccacct
3421 ggtggataaggaacatgacagtgctgaaggaagccatacctctgggcagagtaacgggcg
3481 agaccaccaagcactggccaaaggcggtccaggttcaccaaagacactctgcgcaccaat
3541 gtactttgcttgagcggccgctcgagtctagagggcccgtttaaacccgctgatcagcct
3601 cgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttga
3661 ccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcatt
3721 gtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggagg
3781 attgggaagacaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcgg
3841 aaagaaccagctggggctctagggggtatccccacgcgccctgtagcggcgcattaagcg
3901 cggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccg
3961 ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctc
4021 taaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaa
4081 aacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgcc
4141 ctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacac
4201 tcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctatt
4261 ggttaaaaaatgagctgatttaacaaaaatttaacgcgaattaattctgtggaatgtgtg
4321 tcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgca
4381 tctcaattagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtat
4441 gcaaagcatgcatctcaattagtcagcaaccatagtcccgcccctaactccgcccatccc
4501 gcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttat
4561 ttatgcagaggccgaggccgcctctgcctctgagctattccagaagtagtgaggaggctt
4621 ttttggaggcctaggcttttgcaaaaagctcccgggagcttgtatatccattttcggatc
4681 tgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcagg
4741 ttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcgg
4801 ctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaa
4861 gaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggct
4921 ggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggga
4981 ctggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgc
5041 cgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctac
5101 ctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagc
5161 cggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaact
5221 gttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcga
5281 tgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtgg
5341 ccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctga
5401 agagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccga
5461 ttcgcagcgcatcgccttctatcgccttcttgacgagttcttctgagcgggactctgggg
5521 ttcgaaatgaccgaccaagcgacgcccaacctgccatcacgagatttcgattccaccgcc
5581 gccttctatgaaaggttgggcttcggaatcgttttccgggacgccggctggatgatcctc
5641 cagcgcggggatctcatgctggagttcttcgcccaccccaacttgtttattgcagcttat
5701 aatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactg
5761 cattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctgtataccgtcg
5821 acctctagctagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttat
5881 ccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcc
5941 taatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcggga
6001 aacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgt
6061 attgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcgg
6121 cgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataac
6181 gcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcg
6241 ttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctca
6301 agtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagc
6361 tccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctc
6421 ccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtag
6481 gtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgcc
6541 ttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggca
6601 gcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttg
6661 aagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctg
6721 aagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgct
6781 ggtagcggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaa
6841 gatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaaggg
6901 attttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatga
6961 agttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgctta
7021 atcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactc
7081 cccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatg
7141 ataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccgga
7201 agggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgt
7261 tgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccatt
7321 gctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcc
7381 caacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttc
7441 ggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggca
7501 gcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgag
7561 tactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcg
7621 tcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaa
7681 cgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaa
7741 cccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtga
7801 gcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttga
7861 atactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatg
7921 agcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
7981 ccccgaaaagtgccacctgacgtc
//