Supplementary Material
Kinetochore function from the bottom up
Stephen M. Hinshaw and Stephen C. Harrison
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and Howard Hughes Medical Institute, 250 Longwood Avenue, Boston, MA 02115, USA.
Table S1 – Ctf19 complex/CCAN proteins
COMPLEX / HUMAN / BUDDING YEAST / FISSION YEAST / HUMAN KNOCKOUT/DOWN / BUDDING YEAST DELETION / FISSION YEAST DELETION / REFERENCESNucleosome / CENP-A / Cse4 / cnp1 / Lethal / Lethal / Lethal / [1-3]
CENP-C / Mif2 / cnp3 / Lethal / Lethal / Lethal/very slow growth, suppressed by Fta1 overexpression / [4-6]
CENP-N/Chl4 / CENP-N / Chl4 / mis15 / Lethal / Chromosomal instability / Lethal / [7-9]
CENP-L / Iml3 / fta1 / Lethal / Chromosomal instability / Lethal / [6, 10]
CENP-I/Ctf3 / CENP-I / Ctf3 / mis6 / Lethal / Chromosomal instability / Lethal / [11, 12]
CENP-H / Mcm16 / fta3 / Lethal / Chromosomal instability / Lethal / [7, 13]
CENP-K / Mcm22 / sim4 / Lethal / Chromosomal instability / Lethal after 4-6 divisions / [10, 14, 15]
CENP-M / Lethal / [10]
COMA / CENP-O / Mcm21 / mal2 / Slow-growing / Chromosomal instability / Lethal after 0-5 divisions / [10, 14, 15]
CENP-P / Ctf19 / fta2 / Slow-growing / Chromosomal instability / Lethal / [10, 16, 17]
CENP-Q / Okp1 / fta7 / Slow-growing / Lethal / Lethal / [7, 18, 19]
CENP-U / Ame1 / mis17 / Slow-growing / Lethal / Lethal / [7, 20]
CENP-R / Slow-growing / [18]
CENP-T/Cnn1 / CENP-T / Cnn1 / cnp20 / Lethal / Chromosomal instability / Lethal / [6, 18]
CENP-W / Wip1 / wip1 / Lethal / Chromosomal instability / Lethal/very slow growth / [18, 21]
Nkp1/2 / Nkp1 / fta4 / Lethal / [7]
Nkp2 / cnl2 / Viable / [22]
Table S2 – Phosphorylation at the kinetochore
KINASE / SUBSTRATE / ORGANISM / FUNCTION / REFERENCEPLK1/Cdc5 / Mis18BP1 / Hs / Positive regulation of Mis18-HJURP targeting for new CENP-A deposition in G1 / [23]
PLK1/Cdc5 / CENP-U/Ame1 / Hs / Positive regulation of CENP-Q/Okp1 phosphorylation through recruitment of PLK1 / [24]
PLK1/Cdc5 / CENP-Q/Okp1 / Hs / Negative regulation of CENP-Q kinetochore localization late in the cell cycle / [25]
PLK1/Cdc5 / Lrs4 / Sc / Positive regulation of monopolin localization. Possibly indirect. Depends on Spo13. / [26, 27]
PLK1/Cdc5 / SA2/Scc3 / Hs / Positive regulation of prophase removal of cohesin from chromosomes / [28]
PLK1/Cdc5 / Rad21/Scc1 / Hs, Sc / Positive regulation of Scc1 cleavage by separase / [28, 29]
PLK1/Cdc5 / BubR1 / Hs, Xl / Required for checkpoint arrest, generation of 3F3/2 epitope / [30-32]
PLK1/Cdc5 / PICH / Hs / Positive regulation of Mad2 localization / [33]
PLK1/Cdc5 / Mam1 / Sc / Substrate during meiosis I. Possibly indirect. / [34]
PLK1/Cdc5 / Survivin / Hs / Activation of Aurora B activity at centromeres / [35]
PLK1/Cdc5 / CLASP-2 / Hs / Required for proper chromosome alignment / [36]
Cdk1 / Mis18BP1 / Hs / Negative regulation of CENP-A assembly in G2/M / [37, 38]
Cdk1 / HJURP / Hs / Negative regulation of CENP-A assembly in G2/M / [38, 39]
Cdk1 / CENP-T/Cnn1 / Hs, Sc / Positive regulation of Ndc80 recruitment; negative regulation of Mis12/MIND binding / [40-44]
Cdk1 / CENP-A/Cse4 / Hs / Negative regulation of CENP-A-HJURP interaction, prevents CENP-A deposition in G2/M / [45]
Cdk1 / Dsn1 / Sc / Unknown, antagonized by Cdc14 prior to anaphase / [46]
Cdk1 / Aurora B/Ipl1 / Sc / Negative regulation of Bim1 interaction / [47]
Cdk1 / INCENP/Sli15 / Hs, Sc / Negative regulation of CPC spindle localization / [48, 49]
Cdk1 / Survivin/Bir1 / Sp / Positive regulation of centromere localization through Sgo1 binding / [50]
Cdk1 / Borealin / Hs / Positive regulation of centromere localization through Sgo1 binding / [50]
Cdk1 / RepoMan / Hs / Negative regulation PP1-RepoMan localization before anaphase / [51]
Cdk1 / BubR1 / Xl / Positive regulation of checkpoint arrest and Mad2 recruitment, prerequisite for PLK1 activity against BubR1 / [52]
Cdk1 / PICH / Hs / Positive regulation of PLK1-PICH interaction / [33]
Cdk1 / CLASP-2 / Hs / Positive regulation of PLK1-CLASP-2 interaction, involved in PLK1 targeting to kinetochores. / [36]
Cdk1 / Astrin / Hs / Positive regulation of Astrin kinetochore localization / [53]
Cdk1 / Ska3 / Hs / Positive regulation of Ska3-Ndc80 interaction / [54]
Mps1 / CENP-T/Cnn1 / Sc / Positive regulation of Ndc80 recruitment; negative regulation of Mis12/MIND binding / [40, 44, 55]
Mps1 / KNL1/Spc105 / Sc, Hs / Positive regulation of SAC protein recruitment to KNL1/Spc105. / [56-58]
Mps1 / Borealin / Hs / Positive regulation of chromosome alignment through Aurora B / [59]
Mps1 / Bub1 / Sc / Positive regulation of Mad1 kinetochore recruitment and SAC activity / [60]
Mps1 / Ska3 / Hs / Positive regulation of Ska complex dissociation from the microtubule plus end / [61]
Aurora B/Ipl1 / Dsn1 / Hs, Sc / Positive regulation of kinetochore assembly through CENP-C/Mif2 interaction / [62]
Aurora B/Ipl1 / KNL1/Spc105 / Hs, Sc, Ce / Negative regulation of KMN-MT interaction / [63]
Aurora B/Ipl1 / CENP-C/Mif2 / Sc / In vitro phosphorylation, function unknown / [64]
Aurora B/Ipl1 / CENP-A/Cse4 / Hs / Positive regulation of kinetochore assembly / [65-67]
Aurora B/Ipl1 / INCENP/Sli15 / Sc, Ce / Negative regulation of CPC spindle localization. / [68, 69]
Aurora B/Ipl1 / Survivin/Bir1 / Hs / Positive regulation of Survivin localization / [70]
Aurora B/Ipl1 / Ndc80c (Ndc80, Hec1) / Hs, Sc, Ce / Negative regulation of MT binding / [71-73]
Aurora B/Ipl1 / DASH (Dam1, Spc34, Ask1) / Sc / Negative regulation of KT-MT interaction / [73]
Aurora B/Ipl1 / Ska1/3 / Hs / Negative regulation of kinetochore interactions / [74]
Aurora B/Ipl1 / Cep3 / Sc / In vitro phosphorylation, function unknown / [64]
Aurora B/Ipl1 / EB1/Bim1 / Sc / Positive regulation of midzone disassembly at anaphase / [47]
Aurora B/Ipl1 / Mad3 / Sc / Positive regulation of SAC signaling / [75]
Aurora B/Ipl1 / histone H3 / Hs, Sc, Ce, Xl / Anaphase chromatin compaction in yeast. / Reviewed in [76]
Aurora B/Ipl1 / PLK1 / Dm, Hs / Activation of PLK1 at centromeres / [77]
Aurora B/Ipl1 / zwint-1 / Hs / Positive regulation of dynein recruitment to kinetochores / [78]
Aurora B/Ipl1 / CENP-E / Hs / Regulation of CENP-E activity. Required for normal chromosome congression. / [79]
Cdc7 / Ctf19 / Sc / Positive regulation of Scc2 recruitment in G1 / [80, 81]
Cdc7 / Unknown / Sc / Positive regulation of replication origin activation at centromeres / [80]
Other kinases (see also[82]):
Aurora A
SKAP
Bub1
Casein kinase/Hrr25
Haspin
1
Supplemental References
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