Journal of Industrial Microbiology & Biotechnology
Enhancement of ascomycin productioninStreptomyces hygroscopicusvar. ascomyceticusby combining resin HP20 addition and metabolic profiling analysis
HaishanQia,b,c, SuminZhaoa,b,c, Hong Fud,JianpingWena,b,c,*, XiaoqiangJiaa,b,c
a Key Laboratory of system bioengineering (Tianjin University), Ministry of Education, Tianjin, 300072,People’s Republic of China
b School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
c Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), 300072, People’s Republic of China
d Tianjin Vocation Technology College of bioengineering,Tianjin 300462, People’s Republic of China
*Author for correspondence: Tel. & Fax: +86-22-27892061；
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Table S1 Annotated and classified metabolites detectedby GC-MS and LC-MS/MS in S. hygroscopicus var.ascomyceticus FS35cultures.
Method / Compound / Match percent (%) / ClassGC-MS / Hexadecanoic acid / 91 / Organic acid
α-ketoglutarate / 94
Pipecolic acid / 93
Dodecanoic acid / 90
Citrate / 89
Oxaloacetate / 91
Octadecanoic acid / 97
Oleic acid / 89
Pyruvate / 95
Hexanoic acid / 98
Hexanedioic acid / 78
Ketoadipic acid / 93
Methylmalonic acid / 92
Fumarate / 87
Tetradecanoic acid / 79
Mannonic acid / 94
Decanoic acid / 93
α-Aminoadipic acid / 86
Gluconic acid / 87
Propanoic acid / 86
Malate / 82
Benzoic acid / 87
Glyoxylic acid / 84
Lactate / 96
Malonic acid / 94
Acetate / 97
Succinate / 96
Butyrate / 79
Lysine / 95 / Amino acids
Tryptophan / 87
Phenylalanine / 96
Tyrosine / 90
Leuine / 89
Valine / 92
Asparagine / 94
Glutamate / 91
Glutamine / 92
Isoleucine / 97
Proline / 96
Cysteine / 88
Methionine / 87
Glycine / 74
Aspartate / 91
Threonine / 90
Alanine / 87
Serine / 94
GC-MS / Heptulose / 91 / Sugars
Glucose / 92
Mannose / 77
Maltose / 79
Ribose / 96
Inositol / 85
2-Deoxyribose / 89
Galactose / 95
Talose / 95
Fructose / 88
4-Ketoglucose / 91
Phenylethanolamine / 91 / Other compounds
Butanediamine / 76
Adenosine / 85
Uridine / 94
Pyrimidine / 88
Phosphoric acid / 77
Glycerol / 73
Galactitol / 84
Sorbitol / 76
LC-MS/MS / Fructose-1,6-diphosphate / / / Phosphate compounds
Glyceraldehyde-3-phosphate / /
Phosphoenolpyruvate / /
6-phosphogluconate / /
Xylulose-5-phosphate / /
Ribulose-5-phosphate / /
Erythrose-4-phosphate / /
Sedoheptulose-7-phosphate / /
Glucose-6-phosphate / /
Fructose-6-phosphate / /
3-phosphoglycerate / /
Nicotinamide adenine dinucleotide / /
Nicotinamide adenine dinucleotide phosphate phosphate;phosphate; / /
Reduced nicotinamide adenine dinucleotide / /
Adenosine-5-triphosphate / /
Adenosine-5-diphosphate / /
Dihydroxy-acetone-phosphate / /
Fig. S1Schematic diagram of the proposed metabolic pathways closely associated with ascomycin biosynthesis inS. hygroscopicus var.ascomyceticus. 3PG, 3-phospho-glycerate; 6PG, 6-phospho-gluconate; ACCOA, acetyl-CoA; AKG, α-ketoglutarate; Ala, alanine; Arg, arginine; Asp, aspartate; CHOR, chorismate;CIT, citrate; Cys, cysteine; DHCHC, (4R,5 R)-4,5-dihydroxycyclohex-1-enecarboxylic acid; E4P, erythrose-4-phosphate; F6P, fructose-6-phosphate; FUM, fumarate; G3P,glyceraldehyde-3-phosphate; G1P, glucose-1-phosphate; G6P, glucose-6-phosphate; Gln, glutamine; Glu, glutamate; Gly, glycine; His, histidine; Ile, isoleucine; LAC, lactate; Leu,leucine; Lys, lysine; OAA, oxaloacetate; PEP, phosphoenolpyruvate; Phe,phenylalanine; PHEN, prephenic acid; Pro, proline; PYR, pyruvate; R5P, ribose-5-phosphate; RL5P, ribulose-5-phosphate; S7P, sedoheptulose-7-phosphate; Ser,serine; SME, shikimate;SUCC,succinate;SUCCOA, succiny-CoA; Thr, threonine; Trp, tryptophan; Tyr, tyrosine; Val, valine; X5P, xylulose-5-phosphate.