Supplementary Material

for

A genome-wide association study of severe teenage acne in European Americans

Supplementary Methods

GWAS Discovery set:

1) Breast cancer cases inthe Cancer Genetic Markers of Susceptibility project (CGEMS): Eligible cases in this study consisted of women with pathologically confirmed incident breast cancer from the sub-cohort who gave a blood specimen. Cases with a diagnosis after blood collection up to June 1, 2000 with no previously diagnosed cancer except for non-melanoma skin cancer were included (Hunter et al. 2007).

2) Kidney stone case-control study:The biennial questionnaires have asked whether a participant had been diagnosed with a kidney since 1991 in NHS II. We mailed a supplementary questionnaire to those reporting a first diagnosis of nephrolithiasis to obtain additional information, including symptoms and stone type. Since 2002, we have been requesting permission to obtain medical records related to the diagnosis of nephrolithiasis from newly diagnosed cases (Curhan and Taylor 2008). Review of medical records has confirmed the diagnosis of stone disease in over 90% of the cases.Details regarding the confirmation of kidney stone disease were published previously (Taylor et al. 2005).

GWAS Replication set:

1) Renal function study: Detailed population description of the participants from renal function study was published previously (Forman et al. 2008). The study population is a sub-cohort of the NHSII. With the originalintent of examining the association between analgesic intakeand decline in kidney function, the populations were first limitedto those who provided blood samples in 1997, then restricted to those who returned supplementaryquestionnaires about analgesic use, and finally to those indicatinga willingness to provide additional future blood samples.Women with prevalent hypertension or those reporting use of antihypertensive medications at the time of urine collection (between the years 1996 and 1998) were excluded, as were women with diabetes.

REFERENCES

Curhan GC, Taylor EN (2008) 24-h uric acid excretion and the risk of kidney stones. Kidney Int 73: 489-96

Forman JP, Fisher ND, Schopick EL, Curhan GC (2008) Higher levels of albuminuria within the normal range predict incident hypertension. J Am Soc Nephrol 19: 1983-8

Hunter DJ, Kraft P, Jacobs KB, Cox DG, Yeager M, Hankinson SE, Wacholder S, Wang Z, Welch R, Hutchinson A, Wang J, Yu K, Chatterjee N, Orr N, Willett WC, Colditz GA, Ziegler RG, Berg CD, Buys SS, McCarty CA, Feigelson HS, Calle EE, Thun MJ, Hayes RB, Tucker M, Gerhard DS, Fraumeni JF, Jr., Hoover RN, Thomas G, Chanock SJ (2007) A genome-wide association study identifies alleles in FGFR2 associated with risk of sporadic postmenopausal breast cancer. Nat Genet 39: 870-4

Taylor EN, Stampfer MJ, Curhan GC (2005) Obesity, weight gain, and the risk of kidney stones. JAMA 293: 455-62

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