Supplementary Table 1
Genes of Methylobacillus flagellatus involved in methylotrophy and respective proteins
GeneGene nameProtein/MetabolicCoordinates on
Functionmodulethe chromosome
Mfla129ppiribose 5-phosphate isomeraseRuMP-A138761-139420
Mfla250hpshexulose phosphate synthaseRuMP260347-261033
Mfla547mauFTTQ biosynthesisMAU571586-572758
Mfla548mauBMADH large subunitMAU572820-574022
Mfla549mauEessential for small subunit maturationMAU574035-574601
Mfla550mauDessential for small subunit maturationMAU574594-575211
Mfla551mauAMADH small subunitMAU575235-575795
Mfla552mauGTTQ biosynthesisMAU575824-576825
Mfla553mauLunknownMAU579829-577305
Mfla554mauMunknownMAU577315-578019
Mfla555mauNunknownMAU578025-578876
Mfla556azuazurin, electron acceptor from MADHMAU579009-579608
Mfla718fdhEformate dehydrogenase delta subunitFOX746334-746552
Mfla719fdhDformate dehydrogenase accessory proteinFOX746555-747391
Mfla720fdhAformate dehydrogenase alpha subunitFOX747394-750243
Mfla721fdhBformate dehydrogenase beta subunitFOX750425-751975
Mfla722fdhCformate dehydrogenase gamma subunitFOX751972-752442
Mfla734pqqFPQQ biosynthesisMOX765408-766820
Mfla735pqqGPQQ biosynthesisMOX766810-768150
Mfla759edd6-phosphogluconate dehydrataseRuMP-A799257-801131
Mfla760eda2-keto 3-deoxy 6-phosphogluconate aldolaseRuMP-A801290-801919
Mfla917zwfglucose 6-phosphate dehydrogenaseRuMP964768-966246
Mfla918gndA6-phosphogluconate dehydrogenase (NAD)RuMP-O966310-967221
Mfla919pgl6-phosphogluconolactonaseRuMP967370-968077
Mfla962ppiribose 5-phosphate isomeraseRuMP-A1008453-1009145
Mfla1061zwfglucose 6-phosphate dehydrogenaseRuMP1107800-1109278
Mfla1062gndA6-phosphogluconate dehydrogenase (NAD)RuMP-O1109342-1110253
Mfla1063pgl6-phosphogluconolactonaseRuMP1110402-1111109
Mfla1106ppiribose 5-phosphate isomeraseRuMP-A1151485-1152177
Mfla1325pgiglucose 6-phosphate isomeraseRuMP147927-1409522
Mfla1579orf22biosynthesis of H4MPTH4MPTOX1692014-1692724
Mfla1580orf19biosynthesis of H4MPTH4MPTOX1692721-1693296
Mfla1581orf20biosynthesis of H4MPTH4MPTOX1693293-1694738
Mfla1582afpdihydromethanopterin reductaseH4MPTOX1694783-1695331
Mfla1647orf21biosynthesis of H4MPTH4MPTOX1762242-1762871
Mfla1648pabBpara-aminobenzoate synthase component IH4MPTOX1762858-1764240
Mfla1649orf9biosynthesis of H4MPTH4MPTOX1764234-1765313
Mfla1650orf1unknownH4MPTOX1765306-1766205
Mfla1651orf17unknownH4MPTOX1766330-1767034
Mfla1652faeformaldehyde activating enzymeH4MPTOX1767122-1767658
Mfla1653hpihexulosephosphate isomeraseRuMP1768011-1768556
Mfla1654hpsgexulosephosphate synthaseRuMP1768673-1769302
Mfla1655taltransaldolaseRuMP-A1769450-1770400
Mfla1656orf7unknownH4MPTOX1770535-1771380
Mfla1657orf5biosynthesis of H4MPTH4MPTOX1771377-1772303
Mfla1658mchmethenyl H4MPT cyclohydrolaseH4MPTOX1772303-1773286
Mfla1659orfYunknownH4MPTOX1773359-1774423
Mfla1660mtdBmethylene H4MPT dehydrogenaseH4MPTOX1774481-1775380
Mfla1661mptGbeta-ribofuranosylaminobenzene 5-phosphate synthaseH4MPTOX1775425-1776357
Mfla1662fhcBformyltransferase/hydrolase complex subunit BH4MPTOX1776698-1778002
Mfla1663fhcAformyltransferase/hydrolase complex subunit AH4MPTOX1778024-1779700
Mfla1664fhcDformyltransferase/hydrolase complex subunit DH4MPTOX1779700-1770608
Mfla1665fhcCformyltransferase/hydrolase complex subunit CH4MPTOX1770605-1771414
Mfla1680pqqEPQQ biosynthesisMOX1796982-1798175
Mfla1681pqqDPQQ biosynthesisMOX1798229-1798429 Mfla1682 pqqC PQQ biosynthesis MOX 1798521-1799273
Mfla1683pqqBPQQ biosynthesisMOX1799285-1800202
pqqAPQQ biosynthesisMOX1800287-1800358
Mfla2034mxaDunknownMOX2175535-2176038
Mfla2035mxaLCa2+ insertion into MDHMOX2176035-2177063
Mfla2036mxaKCa2+ insertion into MDHMOX2177060-2177632
Mfla2037mxaCCa2+ insertion into MDHMOX2177617-2178618
Mfla2038mxaACa2+ insertion into MDHMOX2178615-2179487
Mfla2039mxaSunknownMOX2179487-2180350
Mfla2040mxaRunknownMOX2180382-2181401
Mfla2041mxaIMDH small subunitMOX2181535-2181810
Mfla2042mxaGcytichrome c, electron acceptor from MDHMOX2181894-2182388
Mfla2043mxaJunknownMOX2182415-2183257
Mfla2044mxaFMDH large subunitMOX2183348-2185147
Mfla2249tkttransketolaseRuMP-A2398308-2400299
Mfla2472rperibulosephosphate 3-epimeraseRuMP-A2651671-2652369
Mfla2543faeformaldehyde activating enzymeH4MPTOX2735722-2736258
Mfla2599gndB6-phosphogluconate dehydrogenase (NADP)RuMP-O2798798-2800369
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RuMP, ribulosemonophosphate cycle; RuMP-A, assimilatory ribulosemonophosphate cycle; RuMP-O, oxidative ribulosemonophosphate cycle; TTQ, triptophan triptophylquinone; PQQ, pyrroloquinoline quinone; MAU, methylamine utilization, including TTQ synthesis; MOX, methanol oxidation, including PQQ synthesis; FOX, formate oxidation; H4MPTOX; tetrahydromethanopterin-linked formaldehyde oxidation; MDH, methanol dehydrogenase; MADH, methylamine dehydrogenase. Highlight/no highlight features separate different gene clusters.