Derek A. Wainwright, Ph.D.

Professional Summary

Education:

2009-Pres.Post-Doctoral Fellow (Department of Surgery: The Brain Tumor Center), University of Chicago, Chicago, IL – Concentration(s):Neuro-Oncology /Immunotherapy(Mentor: Dr. Maciej S. Lesniak, M.D.)

2004-2009Doctorate of Philosophy (Cell Biology, Neurobiology and Anatomy), Loyola University Chicago, Chicago, IL – Concentration:Cell Biology / Neuroscience/Immunology(Mentor: Dr. Kathryn J. Jones, Ph.D.)

2002-2004Master of Science (Cell Biology, Neurobiology and Anatomy), Loyola University Chicago, Chicago, IL – Concentration:Orthopedics

(Mentor: Dr. Frederick H. Wezeman, Ph.D.)

1998-2002Bachelor of Science (Biology), Bradley University, Peoria, IL

[Mentor: Dr. Samuel Fan, Ph.D. (1954-2010)]

1998-2001SICU Unit secretary/Nursing Assistant/EKG Technician, TrinityMedicalCenter, Rock Island, IL

Professional Profile

Post-Doctoral Fellow, University of Chicago, The Brain Tumor Center 2009-Present

  • Mentored neurosurgeons, neurosurgery residents and medical students
  • Published research manuscripts on neuro-oncology and immunotherapy
  • Vice President, University of Chicago Postdoctoral Association
  • Member of professional scientific societies; elected to serve on committees
  • Received independent support from the National Institutes of Health (NINDS)
  • Bred and created novel transgenic mouse strains
  • Multi-project management and co-ordination with a publication schedule
  • Delivered international award-winning seminars
  • Established and managed collaborations
  • Oversaw an operating budget
  • Performed complex small animal surgeries and dissections

Doctorate of Philosophy, LoyolaUniversityChicago, Department of 2004-2009

Cell Biology, Neurobiology and Anatomy

  • Teaching Assistant (Medical Histology) – Directed by Dr. John Clancy Jr.
  • Teaching Assistant (Advanced Neurobiology) – Directed by Dr. Keith N. Fargo
  • Wrote and published manuscripts
  • Outlined, produced and presented lectures locally and nationally to scientific audiences
  • Managed research projects related to neurodegenerative disease/injury
  • Performed complex small animal surgeries and dissections
  • Coordinated complex transgenic mouse collaborations with other major medical

centers

  • Received independent support from the Les Turner ALS Foundation
  • Independently wrote and submitted federally required animal use protocols
  • Wrote and published a dissertation

Master of Science, Loyola University Chicago, Department of 2002-2004

Cell Biology, Neurobiology and Anatomy

  • Departmental graduate representative
  • Medical center graduate representative to the all-school graduate council
  • Managed research projects related to osteoporosis
  • Performed complex small animal surgeries and dissections
  • Wrote and published a thesis

Bachelor of Science, Bradley University, Department of Biology 1998-2002

  • First chair in the Symphonic Band
  • Performer in the Peoria Symphony Orchestra
  • Speech team (ranked 1st in the nation) award winner
  • Research focused on Drosophila fruit fly development

SICU Unit secretary/Nursing Assistant/EKG Technician, 1998-2001 Trinity Medical Center

  • Receptionist/secretary for the MICU and SICU
  • Maintained accurate EKG records of patients
  • Routinely gave 12-lead EKGs
  • Bathed patients
  • Assisted physicians during in-room procedures
  • Processed and entered physician orders
  • Took BPs via stethoscope and processed blood gases
  • Maintained in-unit pharmacy stores
  • Transported patients throughout the hospital

Leadership:

2002-2004Departmental representative to the Graduate Student Council

2002-2004Graduate Student Council representative to the Medical Student Council

2010-Pres.Member, American Association of Anatomists – Membership Committee

2010Session Chair, Stress and Immunity: 3rd Annual Brain, Behavior and Immunity Conference, Chicago, IL, USA

2011Invited Panelist, Loyola University Chicago Career Development Series Speaker, “To Do, or Not To Do, THE POST-DOC!”, Chicago, IL, USA

2011-Pres.Member, American Association of Anatomists – Public Affairs Committee

2011Invited Speaker, Riverdale High School Commencement Address, Port Byron, IL, USA

2011Invited Panelist, American Association of Anatomists – Strategic Thinking for Undergraduate Recruitment Task Force, Washington D.C., USA

2011-Pres.Chair, The University of Chicago Post-Doctoral Association – Public Affairs Committee

2011Session Chair, Nutrition, Exercise and Stress: 4th Annual Brain, Behavior and Immunity Conference, Chicago, IL, USA

2012-Pres.Member, National Post-Doctoral Association – Advocacy Committee

2012Invited Panelist, University of Chicago Post-Doctoral Association Career Development Series: Grant Writing Panel, “Tips for a successful fellowship application”

2012-Pres.Vice-President, University of Chicago Biological Sciences Division Postdoctoral Association.

2012Session Chair, Brain Tumor Immunotherapy: 18th Annual Neuro-Tumor Club Dinner Meeting at AACR, Chicago, IL, USA

2012-Pres.Co-Organizer, 6th Annual Brain, Behavior and Immunity Conference, Chicago, IL, USA [The 2013 meeting]

2012Invited Panelist, Forward Focus Workshop: Strategic Planning for the NIH Common Fund, Sponsored by the National Institutes of Health, Elk Grove, IL, USA

Honors

2008(Runner-Up) American Association of Anatomists – National Langman Graduate Student Platform Presentation, Experimental Biology, San Diego, CA, USA

2008(Travel Award) American Association of Anatomists, Experimental Biology, San Diego, CA, USA

2010(1st Place) The Department of Surgery 17th Annual Charles B. Huggins Research Symposium Poster Presentation Competition, The University of Chicago, Chicago, IL, USA

2011(1st Place) Abstract Book Cover Art: Inaugural Post-Doctoral Association Symposium, The University of Chicago, Chicago, IL, USA

2011(1st Place) The Department of Surgery 18th Annual Charles B. Huggins Research Symposium Poster Presentation Competition, The University of Chicago, Chicago, IL, USA

2012(Travel Award) American Association of Anatomists, Experimental Biology, Washington D.C., USA

2012(Runner-Up) American Association of Anatomists – National Postdoctoral Fellow Platform Presentation, Experimental Biology, San Diego, CA, USA

Professional Memberships:

American Association of Anatomists (AAA); Congressional Liaison Committee; National

Association for Biomedical Research; American Association for the Advancement of

Science (AAAS); Society for Neuroscience (SFN); The Psychoneuroimmunology Research

Society (PNIRS); The National Postdoctoral Association (NPA); Society for Neuro-Oncology

(SNO).

Lab Skill:

Western blot, in vitro cell culture, laser microdissection, CD4+ T cell differentiation (e.g.

Th1/Th2), IACUC protocol writing, enzyme assays, cell death assays, in vivo neuron survival

analyses, ELISA, FACS, FACS analysis software, mouse/rat surgeries and gross dissection

including removal of brains, cervical lymph nodes, spleen, and thymus, transgenic mouse model

breeding and work (e.g. FoxP3-GFP, Mut3, Rag2-/-, TLR2-/-, CCR3-/-, IDO-/-), RNA/DNA/protein

extraction and quantification, RT-PCR, immunohistochemistry, immunocytochemistry, co-

immunofluorescence (up to 6-color confocal microscopy), light and fluorescent microscopy,

cryosectioning, protein purification, animal reconstitutions adoptive transfers, immunotherapeutic

antibody administration, microarray analysis, analysis of neuron survival (in vivo).

Peer-Reviewed Scientific Manuscripts

  1. Wainwright DA, Xin J, Sanders VM, Jones KJ. 2008 Differential actions of pituitary adenylyl cyclase activating polypeptide and interferon gamma on Th2- and Th1-associated chemokine expression in cultured murine microglia. Journal of Neurodegeneration and Regeneration. 1(1):31-38. PMID: 20448827
  2. Xin J, Wainwright DA, Serpe CJ, Sanders VM, Jones KJ. 2008 Phenotype of CD4+ T cell subsets that develop following facial nerve axotomy. Brain Behavior and Immunity. 22(4):528-37. PMID: 18024079
  3. Wainwright DA, Xin J, Mesnard NA, Sanders VM, Jones KJ. 2009 Th2- and Th1-associated chemokine expression facial motor nucleus after facial nerve transection in a presymptomatic murine model of mSOD1. Journal of Neurodegeneration and Regeneration. 2(1):39-44. PMID: 20436785
  4. Wainwright DA, Xin J, Sanders VM, Jones KJ. 2009 Effects of facial nerve axotomy on Th2- and Th1-associated chemokine expression in the facial motor nucleus of wild-type and pre-symptomatic mSOD1 mice. Journal of Neuroimmunology. 216(1-2):66-75.PMID: 19818514
  5. Wainwright DA, Xin J, Mesnard NA, Beahrs TR, Sanders VM, Jones KJ. 2009 Exacerbation of facial motoneuron loss after facial nerve axotomy in CCR3-deficient mice. ASN Neuro. e00024. PMID: 19922414
  6. Wainwright DA, Xin J, Sanders VM, Jones KJ. 2010 Toll-like receptor 2 and facial motoneuron survival after facial nerve axotomy. Neuroscience Letters. 471(1):10-14. PMID: 20056129
  7. Klarquist J, Denman CJ, Hernandez C, Wainwright DA, Strickland FM, Overbeck A, Mehrotra S, Nishimura MI, Le Poole C. 2010 Reduced skin homing by functional Treg in vitiligo. Pigment Cell Melanoma Res. 23:276-286. PMID: 20175879
  8. Ahmed AU, Rolle CE, Tyler MA, Han Y, Sengupta S, Wainwright DA, Balyasnikova IV, Ulasov IV, Lesniak MS. 2010 Bone marrow mesenchymal stem cells loaded with an oncolytic adenovirus suppress the anti-adenoviral immune response in the cotton rat model. Molecular Therapy. 18(10): 1846-56. PMID: 20588259
  9. Sengupta S, Nandi S, Wainwright DA, Hindi ES, Han Y, Lesniak MS. 2010. shRNA-mediated fibronectin knockdown delays tumor growth in a mouse glioma model. Neoplasia. 12(10):837-47. PMID: 20927322
  10. Xin J, Wainwright DA, Serpe CJ, Sanders VM, Jones KJ. 2010 IL-10 within the CNS is necessary for CD4+ T cells to mediate neuroprotection. Brain, Behavior and Immunity. 25(5): 820-9. PMID: 20723599
  11. Wainwright DA, Sengupta S, Han Y, Lesniak MS. 2010 The presence of IL-17A and T helper 17 cells in experimental mouse brain tumors and human glioma. PLoS One. 23(10): e15390. PMID:21060663
  12. Wainwright DA, Balyasnikova IV, Han Y, Lesniak MS. 2011. BST2 in experimental mouse brain tumors and human glioma. Experimental and Molecular Pathology. 91(1): 440-6. PMID: 21565182
  13. Thaci B, Ulasov IV, Wainwright DA, Lesniak MS. 2011. The Challenge for Gene Therapy: The Innate Immune Response to Adenoviruses. Oncotarget. 2(3):113-21. PMID:21399236
  14. Wainwright DA, Sengupta S, Han Y, Lesniak MS. 2011. Thymus-derived rather than tumor induced regulatory T cells predominate in brain tumors. Neuro-Oncology. 13(12):1308-23. PMID: 21908444
  15. Xin J, Wainwright DA, Mesnard NA, Beahrs TR, Serpe CJ, Alexander T, Sanders VM, Jones KJ. 2011. CD4+ T cell-mediated neuroprotection is independent of T cell-derived BDNF in a mouse facial nerve axotomy model. Brain, Behavior and Immunity. In press. PMID: 22426430
  16. Wainwright DA, Nigam PN, Thaci B, Dey M, Lesniak MS. 2011. Recent developments on immunotherapy for brain cancer. Expert Opinion on Emerging Drugs. 17(2):181-202. PMID: 22533851
  17. Balyasnikova IV, Wainwright DA, Solomaha E, Lee G, Han Y, Thaci B, Lesniak MS. 2012. Development, characterization and implications for a novel antibody that targets the tumor specific antigen, IL-13R2, with high affinity. Journal of Biological Chemistry. Under Review.
  18. Wainwright DA, Balyasnikova IV, Chang AL, Ahmed AU, Moon K, Auffinger B, Han Y, Lesniak MS. 2012. IDO expression in brain tumors increases the recruitment of regulatory T cells and negatively impacts survival.Clinical Cancer Research. Under Review.

Oral Abstracts (selected from > 15)

1.Investigation of chemokines responsible for CD4+ T cell-mediated neuroprotection in a mSOD1 mouse model and facial nerve axotomy. 40th American Society for Neurochemistry, Charleston, SC, USA. 2009

2.The role of regulatory T cells in malignant glioma. 2nd AnnualBrain, Behavior and Immunity Conference, Chicago, IL, USA. 2009.

3.Malignant glioma recruits multiple types of immune cells to the brain with non-classical molecular phenotypes: relevance to treating neuroinflammation in CNS tumors. 3rd Annual Brain, Behavior and Immunity Conference, Chicago, IL, USA, 2010.

4.Regulating tumor immunity in the brain: IDO takes center stage. 4th Annual Brain, Behavior and Immunity Conference, Chicago, IL, USA, 2011.

5.Tumor-Derived IDO Affects the Lifespan in a mouse model of glioblastoma. Experimental Biology, San Diego, CA, USA, 2012.

6.Investigating the role of IDO in an experimental mouse brain tumor model.18th Annual Neuro-Tumor Club Dinner Meeting (Sponsored by the Society for Neuro-Oncology) during the American Association for Cancer Research Annual Meeting, Chicago, IL, USA, 2012.

7.IDO expression suppresses anti-tumor immunity through a T cell-dependent mechanism. 17th Annual Society for Neuro-Oncology Scientific Meeting. Washington D.C., USA, 2012.

Poster Abstracts (selected from > 20)

  1. Wainwright DA*,Han Y, Lesniak MS. The role of indoleamine 2,3 dioxygenase in a mouse model of malignant glioma. Society for Neuroscience. Washington D.C., USA. 2011. (*Poster Presenter)
  2. Vasilopoulos T, Kristjansdottir K, Bergerson R, Wainwright DA*. From Paper to Practice: Using Survey Results to Improve the Postdoctoral Experience at the University of Chicago. National Postdoctoral Association Annual Meeting. San Francisco, CA, USA. 2012. (*Poster Presenter)
  3. Wainwright DA*, Balyasnikova IV, Ahmed A, et al. Upregulation of indoleamine 2,3 dioxygenase in brain tumors is associated with decreased survival through a T cell-dependent mechanism. 19th Annual Psychoneuroimmunology Research Meeting. San Diego, CA, USA, 2012. (*Poster Presenter)

Research Support:

Ongoing Research Support

F32NS073366-01A1 Wainwright (PI)2011 – 2014$152,766

NIH/NINDS

Title: The Role of IDO and Th17 in an experimental mouse model of glioma.

Role: PI

Clinical Research Loan Repayment Program 2012 – 2013 PENDING

NIH/NCI

Title: The Role of IDO and Th17 cells in Human GBM and Mouse Models That Recapitulate

Brain Tumors.

Role: PI

Completed Research Support

Young Investigator Award Wainwright (Co-PI)2006 – 2009 $150,000

Les Turner ALS Foundation

Title: The Mechanism of Immune Cell Recruitment to the Central Nervous System Following

Peripheral Nerve Axotomy Using a Murine Model of ALS.

Role: Co-PI

Home Address: 8140 West 26th Street, North Riverside, IL 60546;

Phone: (309)236-8525; Email:

Work Address: 5801 South Ellis Avenue, Chicago, IL 60637;

Mobile: (309)236-8525; Email:

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