Electronic Supplementary Material

ESM Table 1. Polymorphism characteristics of microsatellite loci used to type blue tits. Table shows number of alleles (K), annealing temperature (T), expected heterozygosity (HE), and observed heterozygosity (HO) at each locus (based on 210 individuals).

Locus / K / T (ºC) / HE / HO / Primer origin
Pca2 / 15 / 61 / 0.762 / 0.682 / Dawson et al. 2000
Pca3 / 23 / 55 / 0.885 / 0.868 / Dawson et al. 2000
Pca4 / 13 / 60 / 0.748 / 0.726 / Dawson et al. 2000
Pca9 / 14 / 66 / 0.809 / 0.776 / Dawson et al. 2000
Pca7 / 18 / 60 / 0.906 / 0.894 / Dawson et al. 2000
Pca8 / 51 / 53 / 0.953 / 0.927 / Dawson et al. 2000
Pocc1 / 12 / 56 / 0.845 / 0.787 / Bensch et al. 1997
Pocc6 / 22 / 58 / 0.871 / 0.819 / Bensch et al. 1997
Ase18 / 18 / 60 / 0.871 / 0.812 / Richardson et al. 2000
Pdoμ5 / 25 / 46 / 0.853 / 0.797 / Griffith et al. 1999
Pat-MP2-43 / 10 / 59 / 0.511 / 0.517 / Otter et al. 1998
Mcyμ4 / 17 / 50 / 0.833 / 0.859 / Double et al. 1997
PK11 / 12 / 52 / 0.822 / 0.841 / GenBank Acc. no.: AF041465
PK12 / 21 / 62 / 0.858 / 0.822 / GenBank Acc. no.: AF041466

ESM Table 2. Mean and range for individual measurements and breeding parameters for each study subpopulation.

Parameter / Cabañeros / Quintos de Mora
Individual measurements / Males / Females / Males / Females
Tarsus length / 16.3
(14.7-18.2) / 15.8
(14.6-17.0) / 16.2
(14.8-18.0) / 15.8
(14.7-17.6)
Weight / 9.9
(8.9-11.0) / 9.9
(8.8-10.8) / 9.7
(8.5-11.7) / 9.6
(8.0-10.9)
Crown brightness / 380.6
(225.6-724.1) / 348.5
(152.2-773.2) / - / -
Crown chroma / 0.42
(0.29-0.57) / 0.39
(0.32-0.50) / - / -
Feeding rates / 219.2
(39-360) / 145.8
(39-387) / 108.2
(36-270) / 151.1
(28-345)
Homozygosity by loci / 0.23
(0-0.52) / 0.20
(0-0.47) / 0.18
(0-0.53) / 0.17
(0-0.42)
Breeding parameters
Clutch size / 7.6 (4-12) / 7.8 (3-12)
Spotting distribution / 3.6 (1.7-4.7) / 3.6 (2.0-4.7)
Number of fledglings / 6.0 (1-12) / 6.4 (1-11)

References

Bensch S., Price, T. & Kohn, J. 1997. Isolation and characterization of microsatellite loci in a Phylloscopus warbler. Mol. Ecol. 6, 91-92.

Dawson, D.A., Hanotte, O., Greig, C., Stewart, I. R. K. & Burke, T. 2000. Polymorphic microsatellites in the blue tit Parus caeruleus and their cross-species utility in 20 songbird families. Mol. Ecol. 9, 1941-1944.

Double, M. C., Dawson, D., Burke, T. & Cockburn, A. 1997. Finding the fathers in the least faithful bird: A microsatellite-based genotyping system for the superb fairy-wren Malurus cyaneus. Mol. Ecol. 6, 691-693.

Griffith, S. C., Stewart, I. R. K., Dawson, D. A., Owens, I. P. F. & Burke, T. 1999. Contrasting levels of extra-pair paternity in mainland and island populations of the house sparrow (Passer domesticus): is there an 'island effect'? Biol. J. Linn. Soc. 68, 303-316.

Otter, K., Ratcliffe, L., Michaud, D. & Boag, P. T. 1998. Do female black-capped chickadees prefer high-ranking males as extra-pair partners? Behav. Ecol. Sociobiol. 43, 25-36.

Richardson, D. S., Jury, F. L., Dawson, D. A., Salgueiro, P., Komdeur, J. & Burke, T. 2000. Fifty Seychelles warbler (Acrocephalus sechellensis) microsatellite loci polymorphic in Sylviidae species and their cross-species amplification in other passerine birds. Mol. Ecol. 9, 2226-2231.