Pathogenic Bacteriology, BIO144
Fall 2008
Lecture: T/Th 12:00-12:50 p.m.
Laboratory: MWF 10:00-11:50 a.m & MWF 1:00-2:50 p.m.
Dr. Lindgren, Professor, Dept. Biological Sciences
Office: Humboldt 211E, Phone: 278-6152
Email:
Office hours: M 3:00-4:20
T 3:00-4:20
Text: Salyers, A. and D. Whitt. Bacterial Pathogenesis: A Molecular Approach, 2nd Edition
Laboratory Manual: Lindgren Pathogenic Bacteriology: A clinical and investigative approach
Prerequisites: General Microbiology (BIO139)
Course Description: In both the lecture and the laboratory portions of this upper division microbiology course, we will explore how it is that bacteria cause disease from both the host and the pathogen's perspective. Specific pathogens will be discussed at both the cellular and the molecular level as model systems for bacterial-host interactions with detailed discussions on bacterial factors that promote disease. Current issues in this field of study will also be discussed, including antibiotic resistance and vaccine development.
Below is a list of topics in the order that they will be covered in lecture. Many of the topics listed will require more than one lecture period and are noted as continued (cont’d). If we are ahead of schedule, or it seems particularly pertinent, other topics may be covered.
Date Topic Chapter
Sept. 2 Non-specific immunity of host 4 & 5
4 Non-specific and specific immunity of host 6
(no mini-quiz today)
9 Germ Theory & Disease Terminology 2, pg. 19-24
11 Virulence factors - colonization 8
16 Virulence factors - toxins 9
18 Toxins (cont’d)
23 Regulation of virulence genes hand-out
25 Regulation (cont’d)/Vaccines 7
Sept. 30 Vaccines (cont’d) 7
Oct. 2 Antibiotics: Mechanisms of Action & Bacterial Resistance 10 & 11
7 Lecture EXAM #1
9 Antibiotics: Mechanisms of Action & Bacterial Resistance (CONT’D)
14 & 16 Anaerobic bacteria (2 lectures) (pg 142-5; chap24)
21 Diphtheria pg. 138-142
23 Anthrax 22
28 Streptococci: Streptococcus pneumoniae 18
30 Streptococci: Streptococcus pyogenes – 15
from necrotizing faciitis to Strep throat
Nov. 4 Skin infections (powerpoint) (hand-out)
6 Staphylococci – Skin infections and 14
Toxic Shock Syndrome
11th Veteran’s Day (Since 1938) School closed…Study for your exam.
13 Lecture EXAM #2
18 Cholera 25
20 & 25 Salmonella pathogenesis 26
27 no class - Thanksgiving: Salmonella, Campylobacter or S. aureus
Which will it be at your house???
Dec. 2 Whooping cough 17
4 & 9 Mycobacterium tuberculosis 19
11 Chlamydia 31 & part of 21
Helicobacter and ulcers (time permiting) 23
Dec. 23 FINAL EXAM Tues. Dec. 18, 12:45 a.m. - 2:45 p.m.
Mini-Quizzes
Once a week, for 14 weeks of the course (every week except the first week), there will be a Blue Book Mini-Quiz during the first 3 minutes of Lecture. The quiz will be on the material that was covered during the previous lecture session (unless indicated otherwise, ahead of time, by the instructor). Answers to the mini-quiz will be given immediately after the blue books are turned in. If you do not attend class, are late to class, or leave early on the day of a mini-quiz, you will receive a “0” for that quiz. There are no make-ups for these quizzes. Students will be allowed to drop their two lowest mini-quiz scores. Thus, at the end of the semester, 12 of the 14 quizzes will be averaged and will account for 6% of your final course grade. These quizzes can strongly affect your final course grade.
The mini-quiz grades are as follows:
3 = Correct
2 = Close, but not quite
1 = Nope, better luck next time
0 = absent for the mini-quiz
Each student will need two blue books, one for the “odd” numbered week, one for the “even” numbered week. The instructor and the student will swap these books each week. It is advisable that the student keep track of their book as these questions (or one like it) may be seen again on an exam.
Required materials:
Four 882-E scantron sheets for the lecture and laboratory exams and final exam.
Two Blue Books for lecture “mini-quizzes”
Bacterial Pathogenesis: A Molecular Approach 2nd ed. by A. Salyer's
Items required for lab: Lab coat, $15.00 lab fee, "Sharpie" permanent marker, and
Pathogenic Bacteriology: A clinical and investigational approach by S. Lindgren
Grading: It is important to read the assigned material prior to the lecture or laboratory on that subject. Students will be graded on their knowledge of pathogenic bacteriology based on twelve mini-quizzes, two lecture exams, a semi-cumulative final, and a small group oral presentation on a pathogen of your design. In order to evaluate your understanding and expertise of the applied aspects of this field, students will also be required to give a small group presentation of results from laboratory Exercise #8, and take two laboratory exams. Approximately 40% of the total grade will be based on the laboratory portion of the course.
2 Laboratory exams @ 18 % = 36%
Laboratory presentation = 2%
Design a pathogen = 2%
2 Lecture exams @ 18% = 36%
12 Lecture mini-quizzes @ 0.5% = 6% (14, drop the lowest 2)
Final exam = 18%
Final Course Grade (out of 100)
90 and above = A
80 and above = B Plus and Minus grades will be assigned within these
70 and above = C categories
60 and above = D
General Course Policies:
NO MAKE-UP EXAMS WILL BE GIVEN
Attendance: Laboratory attendance is mandatory. You must contact the instructor at
278-6152 if a major problem arises and you must miss laboratory or a scheduled exam.
Cheating: Cheating on exams or plagiarism on papers will not be tolerated. If the instructor obtains evidence of cheating, the incident will be reported to the chair of Biological Sciences and to the Dean of Students, and the student will be assigned an “F” grade for the course. If there is evidence of plagiarism, the student will receive an “F” grade for the assignment.
Late Items: The grade on items turned in will be lowered one letter grade for each day after the due date that they are received.
Add/Drop Policy: Students will be added with the following priority:
Undergraduate graduating senior majors in Biology that require the course for graduation.
Graduate students or second bachelor degree students that require the course
Undergraduate graduating senior majors in Biology.
Undergraduate students in the following order: Senior, Junior, Sophomore, Freshman.
Students may drop the course on their own through CASPER through Fri. Sept. 12. After this date the student can drop the course but must do so through a petition that the instructor and chair of the department must sign. The last day to drop without a serious and compelling reason is October 10th, the end of the sixth week of instruction (withdrawing from the course after this date requires a petition signed by the department chair and the Dean). If a student has failed to attend the first two lecture and laboratory sessions, they will be administratively dropped.
Graded items for this course will be held for only ONE SEMESTER following the course. After this time, they will be recycled.